The regulatory effects of (p)ppGpp and indole on cAMP synthesis in <i>Escherichia coli</i> cells by N. M. Kashevarova, E. A. Khaova, A. G. Tkachenko

“…The mutual influence of network signaling molecules represented by cells’ natural metabolites, including indole and second messengers (p)ppGpp and cAMP, is hitherto not well understood, being the aim of this study. E. coli parent strain BW25141 ((p)ppGpp+) and deletion knockout BW25141ΔrelAΔspoT which is unable to synthesize (p)ppGpp ((p)ppGpp0) were cultivated in M9 medium supplemented with different glucose concentrations (5.6 and 22.2 mM) in the presence of tryptophan as a substrate for indole synthesis and in its absence. …”
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Molecular Identification and Antimicrobial Potential of Streptomyces Species from Nepalese Soil by Karan Khadayat, Dawa Dindu Sherpa, Krishna Prakash Malla, Sunil Shrestha, Nabin Rana, Bishnu P. Marasini, Santosh Khanal, Binod Rayamajhee, Bibek Raj Bhattarai, Niranjan Parajuli

“…The Streptomyces species-derived ethyl acetate extracts from Nepalese soil demonstrate potential activity against ESBL-producing E. coli. Thus, they are potential candidates for antibiotics manufacturing in the future.…”
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Water remediation and antibacterial characteristics of CdO, graphene oxide and reduced graphene oxide blended CdO nanoparticles – A comparative study by N. Manjula, A.R. Balu, N. Arunkumar, S. Chitra Devi, M. Sriramraj, V. Rajamani, A. Vinith

“…Bacterial growth inhibition quality of CO increased with GO/rGO blending and they show better resistance against B. subtilis (gram ​+ ​ve) than E. coli (gram –ve) bacteria. Compared to GCO, rCO exhibited better photocatalytic and antibacterial properties signifying its potential for water remediation and antibacterial applications.…”
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The Generation and Characterization of Recombinant Protein and Antibodies of Clostridium perfringens Beta2 Toxin by Jin Zeng, Fuyang Song, Yi Yang, Chenjie Ma, Guangcun Deng, Yong Li, Yujiong Wang, Xiaoming Liu

“…A His-tagged rCPB2 with integrity and cytotoxicity of native CPB2 was purified from E. coli expressing system, which exhibited a moderate cytotoxicity on NCM460 human intestinal epithelial cells. …”
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TẠO DÒNG VÀ BIỂU HIỆN GEN nahC MÃ HÓA 1,2-dihydroxynaphthalene dioxygenase CỦA VI KHUẨN Paenibacillus naphthalenovorans 4B1 by Le Thi Ha Thanh, Thị Ái Thoa Nguyễn, Thị Xuân Nguyễn, Vũ Ngọc Thi Trần

“…Kết quả điện di SDS-PAGE cho thấy gen nahC đã được biểu hiện thành công trong E. coli BL21 ở điều kiện cảm ứng 0,5 mM IPTG. Enzyme tái tổ hợp thu được có trọng lượng phân tử xấp xỉ 35 kDa. …”
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Total Phenolic, Flavonoid, Tomatine, and Tomatidine Contents and Antioxidant and Antimicrobial Activities of Extracts of Tomato Plant by Norma Patricia Silva-Beltrán, Saul Ruiz-Cruz, Luis Alberto Cira-Chávez, María Isabel Estrada-Alvarado, José de Jesús Ornelas-Paz, Marco Antonio López-Mata, Carmen Lizette Del-Toro-Sánchez, J. Fernando Ayala-Zavala, Enrique Márquez-Ríos

“…The Pitenza variety extracts inhibited the growth of pathogens such as E. coli O157:H7, Salmonella Typhimurium, Staphylococcus aureus, and Listeria ivanovii, yielding inhibition halos of 8.0 to 12.9 mm in diameter and MIC values of 12.5 to 3.125 mg/mL. …”
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Biochemical properties and substrate specificity of GOB-38 in Elizabethkingia anophelis by Ren Liu, Yang Liu, Jiehui Qiu, Qun Ren, Chunping Wei, Dejin Pan, Jianglong Shi, Peng Liu, DanDan Wei, Tianxin Xiang, Na Cheng

“…Our findings indicate that the enzyme GOB-38 displays a wide range of substrates, including broad-spectrum penicillins, 1–4 generation cephalosporins, and carbapenems, potentially contributing to in vitro drug resistance in E. coli through a cloning mechanism. It is important to highlight that GOB-38 exhibits a distinct active site composition compared to GOB-1/18, featuring hydrophilic amino acids Thr51 and Glu141 at both ends of its active center instead of hydrophobic alanine, potentially indicating a preference for imipenem. …”
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BTS1-knockout Saccharomyces cerevisiae with broad-spectrum antimicrobial activity through lactic acid accumulation by Liu Cong, Yuan Zhou, Yu Zhang, Shanshan Mao, Chaoqun Chen, Liying Wang, Xiao Li, Zuo Zhang, Zuobin Zhu, Ying Li

“…Moreover, infected larvae treated with BTS1-KO S. cerevisiae in Galleria mellonella-E. coli (in vivo) and G. mellonella-S. aureus (in vivo) infection models showed significantly prolonged survival times. …”
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Carbapenem resistance in gram-negative pathogens in an Iranian hospital: high prevalence of OXA-type carbapenemase genes by Setareh Mamishi, Reihaneh Hosseinpour Sadeghi, Sadaf Sajedi Moghaddam, Babak Pourakbari, Shiva Poormohammadi, Maryam Sotoudeh Anvari, Shima Mahmoudi

“…Notable resistance patterns, especially in K. pneumoniae and E. coli, underline the urgent need for proactive interventions, including appropriate antibiotic prescription practices and strengthening of antibiotic stewardship programs.…”
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Pharmacokinetics of Immediate and Sustained Release Cephalexin Administered by Different Routes to Llamas (Lama glama) by Verónica Kreil, Luis Ambros, Ana Paula Prados, Lisa Tarragona, Agustina Monfrinotti, Guillermo Bramuglia, Marcela Rebuelto

“…Cephalexin MIC90 values against staphylococci and E. coli were 1.0 and 8.0 μg/mL, respectively. Our results show that the immediate release formulation (10 mg/kg) would be effective for treating staphylococcal infections administered every 8 h (IM) or 12 h (SC), whereas the sustained release formulation (8 mg/kg) would require the IM or SC administration every 12 or 24 h, respectively.…”
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Synthesis, characterization, In silico, and In vitro studies of CuS–ZnO nanocomposite using mushroom extract by S. Anbazhagi, A. Murugesan, M. Paramanantham, K. Chinnaiah, Karthik Kannan, Nadezhda Palko, K. Gurushankar

“…The CuS–ZnO nanocomposite significantly exhibited antimicrobial activity against the pathogens of S. aureus (21 mm-zone of inhibition) and E.coli (25 mm-zone of inhibition). The cytotoxicity analysis shows a CuS–ZnO Nanoparticles have more cytotoxic effect in U87 cell lines. …”
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Immunogenicity of recombinant fragment of orthopoxvirus p35 protein in mice by Ya. A. Khlusevich, A. L. Matveev, E. P. Goncharova, I. K. Baykov, N. V. Tikunova

“…In this work, recombinant protein р35Δ12 produced in E. coli cells XL1-blue and purified by chromatography was used for two-time immunization of mice. …”
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Evaluation of the Synergistic Antimicrobial Activity of Essential Oils and Cecropin A Natural Peptide on Gram-Negative Bacteria by Filippo Fratini, Chiara Pecorini, Ilaria Resci, Emma Copelotti, Francesca Paola Nocera, Basma Najar, Simone Mancini

“…The FIC Indices (FICI) revealed that the most effective synergies were observed with the combinations <i>Sm</i>EO/<i>Cz</i>EO and <i>Sm</i>EO/<i>Cb</i>EO against <i>E. coli</i>, while against <i>S. enterica</i> Typhimurium the best combinations were <i>Cb</i>EO/<i>Cz</i>EO and <i>Sm</i>EO/<i>Cz</i>EO. …”
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Expression Analysis of Heavy-Chain-Only Antibodies in Cloudy Catshark and Japanese Bullhead Shark by Reo Uemura, Susumu Tanimura, Nao Yamaguchi, Ryuichi Kuroiwa, Gabriel Takashi Andrés Tsutsumi, Toshiaki Fujikawa, Kiyoshi Soyano, Kohsuke Takeda, Yoshimasa Tanaka

“…Consequently, we expressed the recombinant protein of the C4 domain from the cloudy catshark in <i>E. coli</i>, immunized rats, and produced antibodies. …”
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Characterization of Patients with Central Nervous System Infections by Benigno Figueiras Ramos, Ángel Julio Romero Cabrera, Raúl López Fernández, Sandra Borroto Lecuona, Raúl Nieto Cabrera

“…Los factores predisponentes fueron neumonía, sinusitis y alcoholismo; el S. pneumoniae resultó el germen aislado con más frecuencia; la E. Coli mostró mayor letalidad. <strong><br />Conclusiones: </strong>haber presentado degradación de la conciencia (OR=41,735), lesiones purpúricas (OR=6,641) o padecer de una meningoencefalitis bacteriana (OR=22,958), se asociaron de forma independiente con el riesgo de morir.…”
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Photodynamic and photothermal bacteria targeting nanosystems for synergistically combating bacteria and biofilms by Xiao Wang, Wenxuan Shi, Yu Jin, Zhuoyuan Li, Tanjun Deng, Tingshu Su, Ao Zheng, Lingyan Cao

“…Upon exposure to red-light (RL) irradiation, Ce6@ZIF-8-PDA/UBI showed excellent antibacterial effect on S. aureus, E. coli, F. nucleatum, and P. gingivalis and exceptional light-driven antibiofilm activity to P. gingivalis biofilm, which was a result of the efficient bacterial localization mediated by PDA/UBI, as well as the PDT/PTT facilitated by Ce6/PDA interactions. …”
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Characterization of a chitinase from Trichinella spiralis and its immunomodulatory effects on allergic airway inflammation in mice by Jia Xu, Ye Yao, Qisheng Zhuang, Zixuan Li, Min Zhang, Shouan Wang, Hongxin Hu, Jianbin Ye

“…Results Recombinant Ts-chit (rTs-chit) was successfully expressed in E. coli BL21, showing strong structural similarity to murine acidic mammalian chitinase (AMCase). …”
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Exploring green synthesis of parietin-mediated silver nanoparticles using γ-ray induced Xanthoria parietina foliose extracts for antibacterial potential by Sikander Ali, Alisha Moazzam, Saima Shahzad Mirza, Majid Mahmood, Sibtain Ahmed, Muhammad Usman Ahmad, Gildardo Rivera, Akhtar Rasool Asif, Muhammad Arshad, Mirza Imran Shahzad, Kaynat William, Sundas Sharif

“…The free parietin extract and synthesized nanoparticles (Xa-AgNPs) were evaluated for antimicrobial activity against selected bacterial isolates; S. aureus, B. subtilis (gram-positive), E. coli, Pseudomonas sp. (gram-negative) by agar well diffusion method. …”
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Bio- synthesis zinc oxide nanoparticle: Azadirachta indica and Phyllanthus acidus mediated green approach for enhanced biological efficacy by G. Joesna, Abdullah Alodhayb, P. Sasikumar, T. Lalitha Sree, R. Zema Ferin, D. Sankar, A. Prabakaran, M. Prasath, Muthumareeswaran Muthuramamoorthy, M. Gulam Mohamed, M. Vimalan

“…The nanoparticles of ZnO showed prominent antibacterial and antifungal activity against E. coli, S aureus, K pneumoniae, S.typhi, Candida albicans and Aspergillus species respectively. …”
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Exploring phytochemical, antioxidant, and antimicrobial properties of Plumeria pudica Jacq. leaves by Kavan Shukla, Kunal N. Odedra, B. A. Jadeja

“…With that the antimicrobial properties of the leaves were also put to test against four bacterial strains namely, Kocuria rhizophila, Pseudomonas aeruginosa, Klebsiella pneumonia, and E. coli. The results of the phytochemical evaluation indicated that both IPA and hydroalcoholic extracts exhibited a superior phytochemical composition, emphasizing the higher extractive potential of IPA compared to the non-polar petroleum ether extract. …”
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