Overcoming barriers for investigating nickel-pincer nucleotide cofactor-related enzymes by Jorge L. Nevarez, Aiko Turmo, Santhosh Gatreddi, Swati Gupta, Jian Hu, Robert P. Hausinger

“…In addition to applying the Duet expression system for synthesis of active, NPN cofactor-containing enzymes in E. coli, we demonstrate that circular dichroism spectroscopy provides a broadly applicable means of assaying these enzymes. …”
Get full text

HSBM-Produced Zinc Oxide Nanoparticles: Physical Properties and Evaluation of Their Antimicrobial Activity against Human Pathogens by Imen Massoudi, Ridha Hamdi, Ibtisam Ababutain, Ethar Alhussain, Aya Kharma

“…Among the six Gram-negative tested bacteria, S. sonnei showed the largest inhibition zone of about 11.3 ± 0.6 mm with ZNPs measuring 148 nm in size (milled for 2 h), followed by E. coli ATCC 25922. Accordingly, S. aureus was the most susceptible Gram-positive bacteria, with inhibition zone size gradually increasing from 11.8 ± 0.3 mm to 13.5 ± 0.5 mm with decreasing nanoparticle size from 767 to 148 nm, while S. aureus ATCC 25923 was resistant to both milled and unmilled samples. …”
Get full text

Cross-contamination Risks in Dry Produce Packinghouses: Efficacy of Alcohol-based Sanitizers to Reduce Salmonella and Potential Surrogates on Relevant Surface Materials by Alexandra Nerney, Stuart Reitz, Jovana Kovacevic, Joy Waite-Cusic

“…Lower alcohol (<30%) products were ineffective (<2-log reduction). E. coli and Salmonella were comparable in sensitivity to sanitizers, but E. faecium was more sensitive (p < 0.05). …”
Get full text

Assessment of Microbiological Contamination of Branded and Street Vended Ice-Cream: A Comparative Study in Tangail Municipality, Bangladesh by Afroza Khatun, Md Younus Mia, Masuma Masuma, Kamal Kanta Das

“…In case of specific pathogen most of the samples contaminated with <em>E. coli</em> and <em>Staphylococcus aureus</em> were found to be present in branded samples up to 10⁴ cfu/ml and 10⁶ cfu/ml on street samples. …”
Get full text

Etiology, Risk Factors, and Antimicrobial Resistance in Recurrent Pyogenic Liver Abscesses: A Six-Year Analysis by Cui J, Liu Y, Fu H, Cui M, Li J, Bai Z, Li J

“…Multivariate analysis identified diabetes mellitus and cerebral infarction as significant risk factors for recurrent pyogenic liver abscess.Conclusion: In comparison with patients without recurrence, those who experienced recurrent pyogenic liver abscess following initial diagnosis exhibited a higher prevalence of E. coli as a pathogenic bacterium. In addition, a history of diabetes mellitus and cerebral infarction are high-risk factors for with pyogenic liver abscess recurrence for the first time.Keywords: pyogenic liver abscess, relapse, Klebsiella pneumoniae, Escherichia coli, diabetes mellitus…”
Get full text

Isolation, Characterization, and Quantification of Bacteria from African Sausages Sold in Nairobi County, Kenya by W. H. Karoki, D. N. Karanja, L. C. Bebora, L. W. Njagi

“…Five genera of bacteria, namely, Staphylococcus spp. at 50.4%, Bacillus spp. at 19.5%, Streptococcus spp. 9.8%, Proteus spp. 2.4%, and E. coli spp. at 1.6%, were isolated from 80 African sausage samples. …”
Get full text

Synthesis, Characterization, Cyclic Voltammetry, and Biological Studies of Co(II), Ni(II), and Cu(II) Complexes of a Tridentate Schiff Base, 1-((E)-(2-Mercaptophenylimino) Methyl)... by Maurice Kuate, Mariam Asseng Conde, Evans Ngandung Mainsah, Awawou G. Paboudam, Francis Merlin M. Tchieno, Kevin I. Y. Ketchemen, Ignas Tonle Kenfack, Peter T. Ndifon

“…In vitro antibacterial and antifungal activity against five bacterial strains (Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Enterococcus faecalis, and Proteus mirabilis) and five fungal strains (Candida albicans, Candida glabrata, Candida tropicalis, Candida krusei, and Candida parapsilosis) showed no antifungal activity but moderate antibacterial activity on E. coli, S. aureus, P. aeruginosa, and P. mirabilis bacterial strains. …”
Get full text

Antibiofilm and Anti-Quorum Sensing Potential of Safely-Synthesized Hydrated Zirconium Oxide-Coated Alginate Beads against Some Pathogenic Bacteria by Alfred Ngenge Tamfu, Yasemin Ispirli Dogaç, Ozgur Ceylan, Andreea Botezatu Dediu, Selahattin Bozkurt, Rodica Mihaela Dinica

“…Biofilm inhibition percentages were relatively high against S. aureus, E. coli, and C. albicans. It is observed that the increase in the alginate amount from 1.5% to 2.0% improves the antimicrobial, anti-QS, and antibiofilm effects. …”
Get full text

Peces de los ríos Aguas Blancas y Chorrerita, Coclé, Panamá y su relación con características ambientales by Bryan E. Jones A., Marcela E. Pinzón P., Martha de Von Chong, Italo Goti, Juan Antonio Gómez H.

“…Se presentaron condiciones desfavorables en términos de calidad microbiológica del agua en ambos ríos, con E. coli promedio de 1 935 ± 2 221 UFC/100 mL y Coliformes totales de 4 804 ± 5 165 UFC/100 mL, con diferencias estadísticas en este grupo. …”
Get full text

Chemical Constituents and Antimicrobial and Antioxidant Activities of Essential Oil from Dried Seeds of Xylopia aethiopica by Samba Fama Ndoye, Yoro Tine, Insa Seck, Lalla Aicha Ba, Seydou Ka, Ismaila Ciss, Abda Ba, Seynabou Sokhna, Moussa Ndao, Rokhaya Sylla Gueye, Nango Gaye, Abdoulaye Diop, Jean Costa, Julien Paolini, Matar Seck

“…The essential oil exhibited excellent activity against S. aureus, E. faecalis, and C. albicans and moderate activity against E. coli. Among all strains tested, C. albicans showed the best sensitivity with a MIC of 50 mg/mL. …”
Get full text

Toxic effects of polystyrene nanoplastics on MDA-MB-231 breast cancer and HFF-2 normal fibroblast cells: viability, cell death, cell cycle and antioxidant enzyme activity by Hanie Sadeghinia, Parichehr Hanachi, Reihaneh Ramezani, Samaneh karbalaei

“…PS-NH2 showed no antibacterial activity against E. coli or Staphylococcus aureus at sizes of 90, 200, and 300 nm. …”
Get full text

Quality control and microbial analysis of liquid herbal drug formulations marketed in three major cities of South Eastern Nigeria by Michael Onyeukwu, Emmanuel Uronnachi, Chidalu Ikeotuonye, Chibuzor Ijezie, Tochukwu Okeke, Somto Ewuzie, Ifeanyi Nzekwe

“…Approximately 53% of the samples demonstrated no antimicrobial activity against common microorganisms (Candida albicans, E. coli, and S. aureus), contradicting their labeled claims.Conclusion: The findings highlight a lack of regulation and standardization in the production of liquid herbal products, posing substantial health risks to consumers.…”
Get full text

Antibiotic evaluation of the nanocomposites IONs-MWCNTs-Pc and IONs-GO-Pc encapsulated in the biocompatible hydrogel poly(VCL-co-PEGDA) based on photodynamic effect by Coralia Cuadrado, Alex Mantilla, Luis Hidalgo, Diego Punina

“…Results: The synthesized nanocomposites, when irradiated with red light at 630 nm, showed significant antimicrobial activity, resulting in a marked reduction in the viability of S. aureus ATCC 27543, S. aureus ATCC 33591, and E. coli ATCC 971182. Photodynamic studies demonstrated that the IONs-GO-Pc nanocomposite was more efficient in generating singlet oxygen compared to IONs-MWCNTs-Pc, which correlated with its superior antimicrobial performance. …”
Get full text

Detection of Methicillin-Resistant Staphylococcus aureus in Clinical and Subclinical Mastitis in Ruminants and Studying the Effect of Novel Green Synthetized Nanoparticles as One o... by Abeer Mostafa Abdalhamed, Gamil Sayed Gamil Zeedan, Amany Ahmed Arafa, Eman Shafeek Ibrahim, Doaa Sedky, Amani Abdel nabey Hafez

“…Out of 150 mastitic milk samples, the frequency of S. aureus was 38 (25.3%), that of E. coli was 45 (30%), that of Klebsiella spp. Was 7 (4.7%), and that of Streptococcus spp. …”
Get full text

CBP22, a Novel Bacteriocin Isolated from Clostridium butyricum ZJU-F1, Protects against LPS-Induced Intestinal Injury through Maintaining the Tight Junction Complex by Tenghao Wang, Jie Fu, Xiao Xiao, Zeqing Lu, Fengqin Wang, Mingliang Jin, Yizhen Wang, Xin Zong

“…CBP22 showed activity against E. col K88, E. coli ATCC25922, and S. aureus ATCC26923. The effects and potential mechanisms of bacteriocin CBP22 on the innate immune response were investigated with a lipopolysaccharide- (LPS-) induced mouse model. …”
Get full text

Phenotypic and molecular characterization of potential pathogens from raw fish, meat and milk samples sold and consumed in Calabar Metropolis, Cross River State, Nigeria by Joy Chinweokwu Ugwu, Glory Philemon Bebia, Edet Uwem, Ajaba Mathias Okang, Emmanuel O. Ibeneme, Andrew Chijioke Ugwu, Augustine Agorye Unimke, Francisca O. Nwaokorie, James Epoke

“…Results: Cultural technique identified a total of 42 isolates and these were: Morganella sp (n=6), Providencia sp (n=7), Klebsiella sp (n=10), E. coli (n=11) and Salmonella sp (n=8). All the isolates showed multi-drug resistance (MDR) to gentamicin, levofloxacin, clindamycin, ciprofloxacin, cefoxicin, piperacillin-azobactam, amikacin, and amoxicillin-clavulanic acid antibiotics. …”
Get full text

Recombinant short TNF-BD protein from smallpox virus is pharmacologically active in an experimental septic shock model by I. P. Gileva, S. N. Yakubitskiy, I. V. Kolosova, S. N. Shchelkunov

“…Using the method of immobilized metal affinity chromatography, recombinant TNF-BD protein corresponding to the TNF-biding domain of VARV-CrmB protein was purified from E. coli cells. The therapeutic potential of TNF-BD was studied using an experimental model of LPS-induced septic shock. …”
Get full text

Chemical, Physical, and Technological Characteristics of Palm Olein and Canola Oil Blends by Ayman Younes Allam, Zakir Showkat Khan, Mohmad Sayeed Bhat, Bindu Naik, Sajad Ahmad Wani, Sarvesh Rustagi, Tahmeed Aijaz, Mohamed Farouk Elsadek, Tse-Wei Chen

“…In addition, biologically active substances (BASs), which could be formed during the frying process, were tested by measuring the inhibition zone of E. coli growth. PO retarded BAS formation. The blended palm or canola oils resulted in better stability and increased organoleptic characteristics and hence can be suitable as economical and healthy alternatives to pure palm or canola oil.…”
Get full text

Evaluation of the quality characteristics of fermented sausages and sausage-like products sold in kars by Çiğdem Sezer, Aksem Aksoy, Özgür Çelebi, Turgay Deprem, Metin Öğün, Nebahat Bilge Oral, Leyla Vatansever, Abamüslüm Güven

“…Neither Clostridium perfringens nor E. coli O157:H7 were identified in any of the samples. …”
Get full text

Effect of Allium cepa L. on Lipopolysaccharide-Stimulated Osteoclast Precursor Cell Viability, Count, and Morphology Using 4′,6-Diamidino-2-phenylindole-Staining by Tatiane Oliveira, Camila A. Figueiredo, Carlos Brito, Alexander Stavroullakis, Anuradha Prakki, Eudes Da Silva Velozo, Getulio Nogueira-Filho

“…Osteoclast precursor cells (RAW 264.7) were stimulated by Pg LPS (1 μg/mL) and E. coli LPS (1 μg/mL) in the presence or absence of different concentrations of AcE (10–1000 μg/mL) for 5 days at 37°C/5% CO2. …”
Get full text