Recombinase-based amplification coupled with lateral flow chromatography for the specific and sensitive detection and identification of Leishmania major in cutaneous leishmaniasis... by Insaf Bel Hadj Ali, Yusr Saadi-Ben Aoun, Imen Khammeri, Hejer Souguir, Emna Harigua-Souiai, Hamed Chouaieb, Ahmed S. Chakroun, Meryem Lemrani, Aicha Kallel, Kalthoum Kallel, Nabil Haddad, Oussaima El Dbouni, Rhea N. Coler, Rhea N. Coler, Rhea N. Coler, Steven G. Reed, Akila Fathallah-Mili, Akila Fathallah-Mili, Ikram Guizani

“…Here, we aimed for a simple and rapid molecular diagnostic test to detect and identify L. major, a predominant CL causal species, which could be prone to become a control tool at the point of care, in endemic areas, using isothermal recombinase DNA amplification (recombinase polymerase amplification, RPA, or recombinase aided amplification, RAA) coupled to detection by the lateral flow (LF) chromatography on a PCRD cassette.MethodsTo develop an L. major species-specific RPA-LF assay, computational analysis of 70 Leishmania DNA targets, identified through bibliography and database searches, selected five targets. We designed and tested 7 primer pairs/probe sets to specifically amplify L. major DNAs. …”
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Optimal dietary vitamin E level facilitates ovarian development of female largemouth bass (Micropterus salmoides) broodstock by Qiuyu Gao, Qiao Feng, Dong Zhou, Zhili Ding, Changle Qi, Youqin Kong, Qiyou Xu

“…The current study was designed to explore the effects of dietary vitamin E on the ovarian development and health of female largemouth bass (Micropterus salmoides). …”
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Large‐Vessel Occlusion Stroke Knowledge and Training for Stroke Severity Assessment Among Emergency Medical Services Personnel in the United States by Kaiz S. Asif, Robin Novakovic, Thanh N. Nguyen, Santiago Ortega‐Gutierrez, Youngran Kim, Amanda Jagolino‐Cole, Sushanth Aroor, Alicia Castonguay, Prateek Kumar, Ashutosh P. Jhadav, Dileep Yavagal, May Nour, Gabor Toth, Maxim Mokin, Sunil A. Sheth, Mouhammad A. Jumaa, Alhamza Al‐bayati, Mohamed Teleb, Ossama Y. Mansour, Shashvat M. Desai, Joshua Hartman, David S. Liebeskind, Ameer E. Hassan, Brijesh P. Mehta

“…It included 12 multiple‐choice and free‐response questions designed to test participants’ clinical knowledge of LVO, knowledge of stroke center certification levels, prior LVO educational experiences, preferences for educational content delivery, and perspectives on prehospital hurdles to stroke care. …”
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Adaptive spatial-channel feature fusion and self-calibrated convolution for early maize seedlings counting in UAV images by Zhenyuan Sun, Zhenyuan Sun, Zhenyuan Sun, Zhi Yang, Zhi Yang, Yimin Ding, Yimin Ding, Boyan Sun, Boyan Sun, Saiju Li, Saiju Li, Zhen Guo, Zhen Guo, Lei Zhu, Lei Zhu

“…To address these challenges, we developed RC-Dino, a deep learning methodology based on DINO, specifically designed to enhance the precision of seedling counts from UAV-acquired images. …”
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Increasing the meaningful involvement of women in HIV cure-related research: a qualitative interview study in the United States by Karine Dubé, Elizabeth Barr, Morgan Philbin, Amaya Perez-Brumer, Brian Minalga, Beth Peterson, Dawn Averitt, Bridgette Picou, Krista Martel, Cecilia Chung, María Mejía, Martha Cameron, Gail Graham, Lynda Dee, Dázon Dixon Diallo, Ebony Gordon, Anastasia Korolkova, Typhanye Dyer, Judith D. Auerbach, Eileen Scully, Krista L. Dong, Sara Gianella

“…They also expressed a desired for women-centered and holistic clinical trial designs that account for intersectionality. Conclusions Our empirical inquiry extends recent calls to action to increase diversity of people involved in HIV cure research. …”
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Immunomodulatory and anti-inflammatory potential of botanicals bioactive product (PHYTO AX'CELL™) for an improvement of the well-being of laying hens at the peak of production by Alessandro Guerrini, Roberta Salaroli, Augusta Zannoni, Giancarlo Avallone, Francesca Leone, Valentina Serra, Giulia Quaglia, Francesca del Zozzo, Thibaut Chabrillat, Claire Carlu, Caterina Lupini, Doriana Eurosia Angela Tedesco

“…This study was designed to assess the effectiveness of an authorized commercial standardized mixture of Artepillin-C, methyl-salicylates, flavonoids and curcuminoids (PHYTO AX'CELL™) provided intermittently during 8 weeks (wk) of trial to laying hens raised in enriched cages during the peak of the production, in the reduction of inflammation status, improving the immune response, and egg quality. …”
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Risk factors for the delayed discharge from anesthesia intensive care unit: a single-center retrospective study by Yue Qian, Jing Hao, Wei Zhu, Yan Yang, Zhuan-yun Zhang, Lu-yang Zhou, Xiao-ping Gu, Zheng-liang Ma, Yu-e Sun

“…Abstract Background A single-center retrospective study was designed to investigate the risk factors associated with delayed discharge from the Anesthesia Intensive Care Unit (AICU). …”
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Image-based analysis of quantitative morphological characteristics of wild potato tubers using the desktop application SeedСounter by K. A. Ivanova, E. G. Komyshev, M. A. Genaev, A. A. Egorova, K. A. Koloshina, N. A. Chalaya, D. A. Afonnikov, A. V. Kochetov, E. V. Rogozina, S. V. Gerasimova

“…There are no available methods specifically designed for the quantitative analysis of wild potato tuber morphology. …”
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Morphological Comparisons of Adult Worker Bees Developed in Chinese and Italian Honey Bee Combs by Shunhua Yang, Hui Li, Pingqing Wu, Dan Yue, Yulong Guo, Wenzheng Zhao, Kun Dong

“…Workers emerging from the control and treatment combs were designated as control and treatment workers, respectively. …”
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The Complete Inpatient Record Using Comprehensive Electronic Data (CIRCE) project: A team‐based approach to clinically validated, research‐ready electronic health record data by Andrea L. C. Schneider, Jennifer C. Ginestra, Meeta Prasad Kerlin, Michael G. S. Shashaty, Todd A. Miano, Daniel S. Herman, Oscar J. L. Mitchell, Rachel Bennett, Alexander T. Moffett, John Chandler, Atul Kalanuria, Zahra Faraji, Nicholas S. Bishop, Benjamin Schmid, Angela T. Chen, Kathryn H. Bowles, Thomas Joseph, Rachel Kohn, Rachel R. Kelz, George L. Anesi, Monisha Kumar, Ari B. Friedman, Emily Vail, Nuala J. Meyer, Blanca E. Himes, Gary E. Weissman

“…Conclusions The CIRCE Project represents a novel cooperative research model to capture clinically validated EHR data from a large diverse academic health system in the greater Philadelphia region and is designed to facilitate collaboration and data sharing to support learning health system activities. …”
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Deciphering molecular and cellular ex vivo responses to bispecific antibodies PD1-TIM3 and PD1-LAG3 in human tumors by Alfred Zippelius, Petra Herzig, Pratiksha Gulati, Christian Klein, Marta Trüb, Kirsten D Mertz, Robert Rosenberg, Viola Heinzelmann-Schwarz, Mark Wiese, Didier Lardinois, Pablo Umana, Marina Natoli, Klas Hatje, Fabian Junker, Zhiwen Jiang, Iakov I Davydov, Markus Germann, Daniel Marbach, Adrian Zwick, Patrick Weber, Stefan Seeber, Lothar Tietze, Laura Codarri-Deak, Henry Kao

“…Background Next-generation cancer immunotherapies are designed to broaden the therapeutic repertoire by targeting new immune checkpoints including lymphocyte-activation gene 3 (LAG-3) and T cell immunoglobulin and mucin-domain containing-3 (TIM-3). …”
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Pharmacodynamic characteristics and influencing factors of tapentadol for chronic pain relief under dose titration by Liang Xin, Haoxiang Zhu, Haoxiang Zhu, Suping Niu, Xie Han, Hongxian Pang, Jiangfan Li, Ye Hu, Xuhong Wang, Lujin Li, Lujin Li, Yi Fang

“…This model is intended to provide a pharmacodynamic reference for designing rational tapentadol dose titration schemes in clinical research.MethodsRandomized controlled trials assessing the efficacy of tapentadol in the management of chronic pain were retrieved from public databases (PubMed and EMBASE). …”
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Dietary Alpha-Lipoic Acid Alleviated Hepatic Glycogen Deposition and Improved Inflammation Response of Largemouth Bass (<i>Micropterus salmoides</i>) Fed on High Dietary Carbohydra... by Zishuo Fang, Xianwei Pan, Ye Gong, Nihe Zhang, Shiwen Chen, Ning Liu, Naisong Chen, Songlin Li

“…Three iso-nitrogenous and iso-lipidic diets supplementing with 0 g/kg, 0.5 g/kg and 1 g/kg LA (LA0, LA500, LA1000) were designed to feed juvenile largemouth bass on a satiation diet twice daily for eight weeks with each diet feeding to triplicate groups. …”
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Prevalence and risk factors for long COVID among cancer patients: a systematic review and meta-analysis by Hongkun Xu, Hongkun Xu, Tingting Lu, Yajie Liu, Jingqi Yang, Simeng Ren, Baojin Han, Honghao Lai, Long Ge, Jie Liu

“…Further randomized controlled trials with rigorous methodological designs and large sample sizes are needed for future validation.Systematic review registrationhttps://www.crd.york.ac.uk/PROSPERO/, identifier CRD42023456665.…”
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A digital intervention to reduce home-office workers’ sedentary behaviour: protocol for the evaluation of the Click2Move programme, a cluster randomised controlled trial by Judit Bort-Roig, Iris Parés-Salomón, Bette Loef, Cristina Vaqué-Crusellas, Alan Coffey, Arnau Gustems-Morral, Anna M. Señé-Mir, Izabela Luznik, Maja Pajek, Kieran P. Dowd, Anna Puig-Ribera, Karin I. Proper

“…Interventions to reduce sitting time, specifically designed for both the office and home-office environments, are required to address this growing public health issue. …”
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BeatProfiler: Multimodal In Vitro Analysis of Cardiac Function Enables Machine Learning Classification of Diseases and Drugs by Youngbin Kim, Kunlun Wang, Roberta I. Lock, Trevor R. Nash, Sharon Fleischer, Bryan Z. Wang, Barry M. Fine, Gordana Vunjak-Novakovic

“…We developed BeatProfiler, a suite of cardiac analysis tools designed to quantify contractile function, calcium handling, and force generation for multiple in vitro cardiac models and apply downstream machine learning methods for deep phenotyping and classification. …”
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Expression and delivery of HA1-M2e antigen using an innovative attenuated Salmonella–mediated delivery system confers promising protection against H9N2 avian influenza challenge by Ram Prasad Aganja, In-Shik Kim, Hyun-Jin Tae, John Hwa Lee

“…Bioinformatics tools predicted that the HA1 and M2e sequences, which were designed as consensus sequences of South Korean strains (2000–2021), would have high antigenicity and favorable structures. …”
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Status of Digital Health Technology Adoption in 5 Vietnamese Hospitals: Cross-Sectional Assessment by Duc Minh Tran, Nguyen Thanh Dung, Chau Minh Duc, Huynh Ngoc Hon, Le Minh Khoi, Nguyen Phuc Hau, Duong Thi Thu Huyen, Huynh Thi Le Thu, Tran Van Duc, Lam Minh Yen, C Louise Thwaites, Chris Paton

“…In hospital settings, DHTs need to be designed and implemented, considering the local context, to achieve usability and sustainability. …”
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Stabilization and Solidification of Produced Tailings in the Lead Extraction Process Using Brine Leaching Method by Ravanbakhsh Shirdam, Atefeh Nezami

“…In this context, to achieve the necessary strength in the tailings, 16 mixtures containing tailings with varying additions of cement (0-10%), silica fume (0-1.5%), and sand (0-10%) were designed using DX7 software. Cubic samples were prepared with a water to powder ratio of 0.4 (w/p=0.4). …”
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The hydraulic performance and clogging characteristics of a subsurface drip irrigation system operating for five years in the North China plain by Li Zaiyu, Mo Yan, Gao Hao, Gong Shihong, Zhang Yanqun, Li Guangyong, Wu Feng

“…Root intrusions were mostly concentrated at the outlets, with a small portion entering the flow channels, accounting for 4.3–20.1 % of the total flow channel length. Owing to the well-designed pressure regulation and air exhaust system used in this SDI research, along with higher soil moisture in the crop root zone, the negative linear relationship between Dra and mc was most significant (p < 0.01). …”
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