Creation of a recombinant Komagataella phaffii strain, a producer of proteinase K from Tritirachium album by A. B. Beklemishev, M. B. Pykhtina, Ya. M. Kulikov, T. N. Goryachkovskaya, D. V. Bochkov, S. V. Sergeeva, A. R. Vasileva, V. P. Romanov, D. S. Novikova, S. E. Peltek

“…The gene encoding natural proteinase K (PK-w) was designed and optimized for expression in K. phaffii K51. …”
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Community-Based Assessment of Knowledge, Attitudes, and Practices Towards COVID-19: an Epidemiological Survey in Kurdistan Region, Iraq by Zhino Rawf Ali, Bayan Omar Sharif, Aram Salih Mohammed Amin Kamali, Varin Tofiq Abbas, Azad Karim Ahmed, Shaho Osman Mahmood, Chiman Jamal ahmed, Sarwat Ahmed Abdulqader

“…A self- constructed questionnaire was used for data collection and it was designed according guidelines, literatures, and articles concerning COVID-19; which consisted of 40 variables. …”
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Feasibility study of pilot scale vegetable waste composting project for Kundasang community’s waste management program by Noorafizah Murshid, Abu Zahrim Yaser, Junidah Lamaming, Mariani Rajin, Sariah Saalah, Mariana Taliban

“…The benefit-to-cost ratio was used to assess the impact of the techno-economic analysis on the designed and piloted KCCS. Results: In the pilot scale composting condition, temperature reached its highest at 59 ± 7 °C (day 5) and for five consecutive days (day 2 until day 6) in the thermophilic phase. …”
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Bis(7)-harmine derivatives as potential multi-target anti-Alzheimer agents by Hongtao Du, Hongtao Du, Hongtao Du, Fang Ma, Fang Ma, Yuanyuan Cao, Miaoyan Bai, Xinyi Gao, Ziyi Yang, Yang Xu, Yan Yan, Yan Yan

“…In this study, a series of bis(7)-harmine derivatives were designed and synthesized as multifunctional drugs for the treatment of AD.MethodsThe derivatives were synthesized by chemical methods and their structure was confirmed by nuclear magnetic resonance (NMR). …”
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A multigene phylogeny toward a new phylogenetic classification of Leotiomycetes by Peter R. Johnston, Luis Quijada, Christopher A. Smith, Hans-Otto Baral, Tsuyoshi Hosoya, Christiane Baschien, Kadri Pärtel, Wen-Ying Zhuang, Danny Haelewaters, Duckchul Park, Steffen Carl, Francesc López-Giráldez, Zheng Wang, Jeffrey P. Townsend

“…Such studies will ideally include designation of epitypes for the type species of those genera for which DNA is not able to be extracted from the original type specimen, and consideration of morphological characters whenever genetically defined clades are recognized as formal taxa within a classification.…”
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Wildlife trade at the interface between deeply-rooted animal-based traditional medicine and unregulated harvesting of wild animals in West Africa by Stanislas Zanvo, Samson Dognimon, Chabi A. M. S. Djagoun, Jiroux Akpatchémè, Akomian Fortuné Azihou, Bruno Djossa, Bruno Djossa, Etotépé A. Sogbohossou, Brice Sinsin

“…A circular layout was designed to delineate the geographic extent of wildlife trade in terms of supplying sources. …”
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Ground-based Characterization of (98943) Torifune 2001 CC21, the Target of the Hayabusa2# Space Mission by Marcel M. Popescu, Eri Tatsumi, Javier Licandro, Miguel R. Alarcon, Javier Rodríguez Rodríguez, Miquel Serra-Ricart, Julia de León, Joaquín Fernandez Martin, David Morate, Gabriel N. Simion, Bogdan Alexandru Dumitru, Daniel Nicolae Berteşteanu, George Pantelimon Prodan, Masatoshi Hirabayashi

“…The near-Earth asteroid (98943) Torifune, previously designated 2001 CC _21 , is the flyby target of the Hayabusa2 extended mission, nicknamed Hayabusa2 # (Small Hazardous Asteroid Reconnaissance Probe (SHARP)). …”
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Intermediate risk prostate tumors contain lethal subtypes by William L. Harryman, James P. Hinton, Rafael Sainz, Jaime M. C. Gard, John M. Ryniawec, John M. Ryniawec, Gregory C. Rogers, Gregory C. Rogers, Noel A. Warfel, Noel A. Warfel, Beatrice S. Knudsen, Raymond B. Nagle, Juan J. Chipollini, Benjamin R. Lee, Belinda L. Sun, Anne E. Cress, Anne E. Cress

“…The presence of these PCa subtypes in biopsy or radical prostatectomy (RP) tissue can exclude patients from active surveillance and from designation as intermediate risk disease, further underscoring the need for increased molecular understanding of these subtypes for diagnostic purposes. …”
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Beta-alanine supplementation in patients with COPD receiving non-linear periodised exercise training or neuromuscular electrical stimulation: protocol of two randomised, double-bli... by Martijn A Spruit, Emiel F M Wouters, Eline bij de Vaate, Peter Klijn, Frits M E Franssen, Wim Derave, Chris Burtin, Florence N Schleich, Roy Meys, Anouk A F Stoffels, Jana de Brandt, Hieronymus W H van Hees, Maurice J H Sillen, Bram van den Borst, Jacqueline M Otker, Jos Donkers, Maurice Hayot, Pascal Pomiès, Inge Everaert

“…The primary objective of the current studies is to assess the beneficial effects of BA supplementation in enhancing exercise tolerance on top of two types of exercise training (non-linear periodised exercise (NLPE) training or neuromuscular electrical stimulation (NMES)) in patients with COPD.Methods and analysis Two randomised, double-blind, placebo-controlled trials have been designed. Patients will routinely receive either NLPE (BASE-TRAIN trial) or NMES (BASE-ELECTRIC trial) as part of standard exercise-based care during their 8-to-10 week pulmonary rehabilitation (PR) programme. …”
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In Vitro Effect of Estradiol and Progesterone on Ovine Amniotic Epithelial Cells by Annunziata Mauro, Hashimita Sanyal, Angelo Canciello, Paolo Berardinelli, Valentina Russo, Nicola Bernabò, Luca Valbonetti, Barbara Barboni

“…With this aim, the present study has been designed to clarify the impact of estradiol (E2), alone or in combination with P4 (12.5 μM and 25 μM), during oAEC amplification. …”
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Use of approved cefazolin based intramammary dry goat treatment and its effects on the milk microbiota by Delower Hossain, Filippo Biscarini, Valentina Monistero, Paola Cremonesi, Bianca Castiglioni, Anoar Jamai Masroure, Alessandro Marinoni, Sara Fusar Poli, Matteo Mezzetti, Maria Filippa Addis, Valerio Bronzo, Erminio Trevisi, Paolo Moroni

“…Therefore, this study was designed to evaluate the effect of an approved cefazolin-based intramammary treatment on the milk microbiota of Alpine dairy goats during the dry and early lactation periods. …”
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TAK-242 improves sepsis-associated acute kidney injury in rats by inhibiting the TLR4/NF-κB signaling pathway by Yan-mei Xia, Yu-qian Guan, Ji-fang Liang, Wei-dong Wu

“…Objective This study was designed to observe the effect of toll-like receptor 4 (TLR4)/nuclear factor kappa-B (NF-κB) pathway activity on sepsis-associated acute kidney injury (SA-AKI), thereby providing new considerations for the prevention and treatment of SA-AKI.Methods The rats were divided into Sham, cecal ligation and puncture (CLP), CLP + vehicle, and CLP + TAK-242 groups. …”
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Cranioplasty infection in porous hydroxyapatite: Potential antibacterial properties by Ismail Zaed, Corrado Iaccarino, Francesca Faedo, Laura Grillini, Elisabetta Galassi, Alessandro Dotti, Angelo Nataloni, Francesca Carolina Mannella, Andrea Cardia

“…Method: The test method has been designed to evaluate the potential antimicrobial activity of specimens under dynamic contact conditions to overcome difficulties in ensuring contact of inoculum to the specimen surface. …”
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Factors associated with risk analysis for asymptomatic left ventricular diastolic dysfunction in nondialysis patients with chronic kidney disease by Yajuan Gao, Shengnan Chen, Jiani Fu, Cui Wang, Yali Tang, Yongbai Luo, Xiaozhen Zhuo, Xueying Chen, Yan Shen

“…Therefore, exploring and intervention of the factors associated with risk for LVDD is of great importance in reducing the morbidity and mortality of cardiovascular disease (CVD) complications in CKD patients. We designed this retrospective cross-sectional study to collect clinical and echocardiographic data from 339 nondialysis CKD patients without obvious symptoms of HF to analyze the proportion of asymptomatic left ventricular diastolic dysfunction (ALVDD) and its related factors associated with risk by multivariate logistic regression analysis. …”
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Workload-capacity imbalances and their impact on self-management complexity in patients with multimorbidity: a multicenter cross-sectional study by Binyu Zhao, Yujia Fu, Jingjie Wu, Erxu Xue, Chuyang Lai, Dandan Chen, Qiwei Wu, Jianing Yu, Qiaoyu Wu, Zhihong Ye, Jing Shao

“…In the network analysis, socioeconomic factors exhibited the central nodes (p < 0.05).Conclusions Personalized interventions designed to increase capacity and reduce workload are essential for improving self-management in multimorbid patients. …”
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Evaluation of the implementation in primary care of genetic testing for the screening of MODY2 (iMOgene): protocol for an implementation pilot study by Marie-Ève Poitras, Luigi Bouchard, Samuel Legeay, Vanessa Tremblay-Vaillancourt, Amélie Fournier, Josianne Leblanc, Philippe Caron, Catherine Savard, Nathalie Bettez, Karine Tremblay

“…Thus, to evaluate the implementation in primary care of genetic testing for the screening of MODY2 (iMOgene study), an implementation pilot study has been designed supported by the RE-AIM (Reach, Effectiveness, Adoption, Implementation, Maintenance) framework.Method and analysis Two primary care clinics will be involved in the region of Saguenay-Lac-Saint-Jean (Québec, Canada). …”
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Genomic interrogation of invasive group A Streptococcus (iGAS) epidemiology and COVID-19 impacts in Victoria, Australia: a 6-year retrospective studyResearch in context by Jacqueline M. Morris, Jake A. Lacey, Kerrie Stevens, Lamali Sadeesh Kumar, Mathilda Wilmot, Janet Strachan, Marion Easton, Daneeta Hennessy, Tony M. Korman, Andrew J. Daley, Katherine B. Gibney, Adam W.J. Jenney, Steven Y.C. Tong, Benjamin P. Howden, Norelle L. Sherry

“…Phylogenetic analysis identified a previously undescribed emm1 sub-lineage, designated M1Aus, detected in Australia (Victoria and Queensland), Belgium and the United Kingdom. …”
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Decoding the anomalies: a genome-based analysis of Bacillus cereus group strains closely related to Bacillus anthracis by Thuto Gomolemo Magome, Marius Surleac, Marius Surleac, Ayesha Hassim, Cornelius Carlos Bezuidenhout, Henriette van Heerden, Kgaugelo Edward Lekota

“…We recommend isolates with ANI ≥99% to standard reference B. anthracis be designated as typical B. anthracis in GenBank to maintain taxonomic clarity and precision.…”
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MASFNet: Multi-level attention and spatial sampling fusion network for pine wilt disease trees detection by Dong Ren, Meng Li, Ziyu Hong, Li Liu, Jingfeng Huang, Hang Sun, Shun Ren, Pan Sao, Wenbin Wang, Jingcheng Zhang

“…To mitigate these challenges, we propose a multi-level attention and spatial sampling fusion network (MASFNet) to reduce false detection problems. Specifically, we designed a Multi-level Attention (MLAttention) module aimed at capturing features through an expanded receptive field to help the model distinguish between the background and the objects of interest. …”
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Protocol for a process evaluation: face-to-face physiotherapy compared with a supported home exercise programme for the management of musculoskeletal conditions: the REFORM trial by David Wong, Catherine Sherrington, Manuela L Ferreira, Christopher G Maher, Hueiming Liu, Joshua R Zadro, Lisa A Harvey, Hannah G Withers, Joanne V Glinsky, Jackie Chu, Matthew D Jennings, Alison J Hayes, Ian J Starkey, Blake A Palmer, Lukas Szymanek, Jackson J Cruwys, Kitty Duong, Anne Barnett, Matthew J Tindall, Barbara R Lucas, Tara E Lambert, Deborah A Taylor

“…Introduction The REFORM (REhabilitation FOR Musculoskeletal conditions) trial is a non-inferiority randomised controlled trial (n=210) designed to determine whether a supported home exercise programme is as good or better than a course of face-to-face physiotherapy for the management of some musculoskeletal conditions. …”
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