Moscatilin inhibits vascular calcification by activating IL13RA2-dependent inhibition of STAT3 and attenuating the WNT3/β-catenin signalling pathway by Tingting Zhang, Mengmeng Zhu, Jialing Ma, Zhenghong Liu, Zhidan Zhang, Meijie Chen, Yaping Zhao, Huaxin Li, Shengnan Wang, Xiaoning Wei, Wenwen Zhang, Xiaoxiao Yang, Peter J. Little, Danielle Kamato, Hao Hu, Yajun Duan, Baotong Zhang, Jianbo Xiao, Suowen Xu, Yuanli Chen

“…Objectives: This study was designed to test the therapeutic effects and mechanism of Moscatilin (also known as dendrophenol) from Dendrobium huoshanense (an eminent traditional Chinese medicine) in suppressing vascular calcification in vitro, ex vivo and in vivo. …”
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Analytical and clinical validation of a novel MeltPlus TB-NTM/RIF platform for simultaneous detection of Mycobacterium tuberculosis complex, Non-Tuberculous Mycobacteria and rifamp... by Zhuo Wang, Yuanwu Zou, Zihan Wei, Guanghong Bai, Xiaolin Wang, Shaoyi Qu, Jie Shi, Yaping Jiang, Cuijiao Gu

“…BackgroundRapid and accurate diagnosis of tuberculosis, particularly rifampin (RIF)-resistant tuberculosis (RR-TB) and Non-Tuberculous Mycobacteria (NTM), is essential for implementing appropriate proper therapy to benefit patients and improve TB/NTM patient management.MethodsIn this study, we developed a novel MeltPlus MTB-NTM/RIF platform, designed to simultaneously detect Mycobacterium tuberculosis complex (MTBC), NTM and RIF resistance. …”
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Minimally important change on the Columbia Impairment Scale and Strengths and Difficulties Questionnaire in youths seeking mental healthcare by Peter Szatmari, Claire de Oliveira, Myla E Moretti, Kristin Cleverley, Lisa D Hawke, Amy H Cheung, Meaghen Quinlan-Davidson, Clement Ma, Karolin R Krause, Alina Lee, Di Shan, Katherine Tombeau Cost, Jo L Henderson

“…The minimally important change (MIC) indicator designates change as meaningful to patients, yet is rarely reported in youth mental health trials.Objective This study aimed to establish MIC thresholds for two patient-reported outcome measures (PROMs), the Columbia Impairment Scale (CIS) and the Strengths and Difficulties Questionnaire (SDQ), using different estimation methods.Methods A sample of 247 youths (14–17 years) completed the CIS and SDQ at baseline and at 6 months in a youth mental health and substance use trial. …”
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Intestinal Microbiome Changes in Fecal Microbiota Transplant (FMT) vs. FMT Enriched with Lactobacillus in the Treatment of Recurrent Clostridioides difficile Infection by Elvira Garza-González, Soraya Mendoza-Olazarán, Rayo Morfin-Otero, Andrea Ramírez-Fontes, Patricia Rodríguez-Zulueta, Samantha Flores-Treviño, Paola Bocanegra-Ibarias, Héctor Maldonado-Garza, Adrián Camacho-Ortiz

“…FMT in addition with Lactobacillus (FMT-L) for treatment of recurrent Clostridioides difficile infection (R-CDI). Methods. We designed a double-blinded randomized comparative two-arm pilot multicenter study to assess the efficacy and impact in the intestinal microbiome of standard capsules of FMT vs. …”
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The implementation and side effect management of immune checkpoint inhibitors in gynecologic oncology: a JAGO/NOGGO survey by Maximilian Riedel, Helene Herrmann, Thomas Bartl, Anna-Maria Rossner, Anna Tatzber, Chiara Flethe, Dario Zocholl, Barbara Schmalfeldt, Jalid Sehouli, Klaus Pietzner

“…The aim of this survey was to provide a real-world overview of the experiences and the current state of irAE management of ICIs in Germany, Switzerland, and Austria. Methods We designed a questionnaire consisting of 34 items focused on physicans’ clinical experiences with ICIs and their management of irAEs. …”
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Creation of body color mutants by CRISPR/Cas9 gene editing in largemouth bass, Micropterus salmoides by Lina Sun, Tian Gao, Zhiqiang Li, Xiaolong Yang, Zuliang Qin, Mingxiao Ye, Ying Li, Feilong Wang, Deshou Wang

“…The target site was designed on the first exon of tyrb and the second exon of csf1ra, respectively. …”
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Beyond variance: simple random distributions are not a good proxy for intraspecific variability in systems with environmental structure by Girard-Tercieux, Camille, Vieilledent, Ghislain, Clark, Adam, Clark, James S., Courbaud, Benoit, Fortunel, Claire, Kunstler, Georges, Pélissier, Raphaël, Rüger, Nadja, Maréchaux, Isabelle

“…To test the impact of this strong assumption on the outcome of community dynamics models, we designed a simulation experiment where we varied the level of knowledge of the environment in virtual communities, resulting in different relative importance of explained vs unexplained spatial individual variation in performance. …”
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Effectiveness of a web-based virtual journal club to promote medical education (Web-Ed): protocol of a multicentre pragmatic randomised trial by Mohamed Abdelrahman, Neil Cowan, Meera Ramcharn, Bassel H Al Wattar, William Parry-Smith, Sarah McRobbie, Michael Rimmer, Nagla Elfaki, Cheryl Dunlop, Damien Coleburt, Olivia Raglan, Jhia Jiat Teh, Maria Fisher, Nilaani Murugesu, Yazid Jibrel, Matthew Wood

“…Where relevant, we will adjust for predetermined characteristics (age, grade of training and session duration) using multivariate regression analyses.Ethics and dissemination Web-Ed was designed by doctors in training to address their learning needs and evaluate the preferred mode of learning. …”
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Higher education students' perceptions of ChatGPT: A global study of early reactions. by Dejan Ravšelj, Damijana Keržič, Nina Tomaževič, Lan Umek, Nejc Brezovar, Noorminshah A Iahad, Ali Abdulla Abdulla, Anait Akopyan, Magdalena Waleska Aldana Segura, Jehan AlHumaid, Mohamed Farouk Allam, Maria Alló, Raphael Papa Kweku Andoh, Octavian Andronic, Yarhands Dissou Arthur, Fatih Aydın, Amira Badran, Roxana Balbontín-Alvarado, Helmi Ben Saad, Andrea Bencsik, Isaac Benning, Adrian Besimi, Denilson da Silva Bezerra, Chiara Buizza, Roberto Burro, Anthony Bwalya, Cristina Cachero, Patricia Castillo-Briceno, Harold Castro, Ching Sing Chai, Constadina Charalambous, Thomas K F Chiu, Otilia Clipa, Ruggero Colombari, Luis José H Corral Escobedo, Elísio Costa, Radu George Crețulescu, Marta Crispino, Nicola Cucari, Fergus Dalton, Meva Demir Kaya, Ivo Dumić-Čule, Diena Dwidienawati, Ryan Ebardo, Daniel Lawer Egbenya, MoezAlIslam Ezzat Faris, Miroslav Fečko, Paulo Ferrinho, Adrian Florea, Chun Yuen Fong, Zoë Francis, Alberto Ghilardi, Belinka González-Fernández, Daniela Hau, Md Shamim Hossain, Theo Hug, Fany Inasius, Maryam Jaffar Ismail, Hatidža Jahić, Morrison Omokiniovo Jessa, Marika Kapanadze, Sujita Kumar Kar, Elham Talib Kateeb, Feridun Kaya, Hanaa Ouda Khadri, Masao Kikuchi, Vitaliy Mykolayovych Kobets, Katerina Metodieva Kostova, Evita Krasmane, Jesus Lau, Wai Him Crystal Law, Florin Lazăr, Lejla Lazović-Pita, Vivian Wing Yan Lee, Jingtai Li, Diego Vinicio López-Aguilar, Adrian Luca, Ruth Garcia Luciano, Juan D Machin-Mastromatteo, Marwa Madi, Alexandre Lourenço Manguele, Rubén Francisco Manrique, Thumah Mapulanga, Frederic Marimon, Galia Ilieva Marinova, Marta Mas-Machuca, Oliva Mejía-Rodríguez, Maria Meletiou-Mavrotheris, Silvia Mariela Méndez-Prado, José Manuel Meza-Cano, Evija Mirķe, Alpana Mishra, Ondrej Mital, Cristina Mollica, Daniel Ionel Morariu, Natalia Mospan, Angel Mukuka, Silvana Guadalupe Navarro Jiménez, Irena Nikaj, Maria Mihaylova Nisheva, Efi Nisiforou, Joseph Njiku, Singhanat Nomnian, Lulzime Nuredini-Mehmedi, Ernest Nyamekye, Alka Obadić, Abdelmohsen Hamed Okela, Dorit Olenik-Shemesh, Izabela Ostoj, Kevin Javier Peralta-Rizzo, Almir Peštek, Amila Pilav-Velić, Dilma Rosanda Miranda Pires, Eyal Rabin, Daniela Raccanello, Agustine Ramie, Md Mamun Ur Rashid, Robert A P Reuter, Valentina Reyes, Ana Sofia Rodrigues, Paul Rodway, Silvia Ručinská, Shorena Sadzaglishvili, Ashraf Atta M S Salem, Gordana Savić, Astrid Schepman, Samia Mokhtar Shahpo, Abdelmajid Snouber, Emma Soler, Bengi Sonyel, Eliza Stefanova, Anna Stone, Artur Strzelecki, Tetsuji Tanaka, Carolina Tapia Cortes, Andrea Teira-Fachado, Henri Tilga, Jelena Titko, Maryna Tolmach, Dedi Turmudi, Laura Varela-Candamio, Ioanna Vekiri, Giada Vicentini, Erisher Woyo, Özlem Yorulmaz, Said A S Yunus, Ana-Maria Zamfir, Munyaradzi Zhou, Aleksander Aristovnik

“…Higher education institutions' managers and teachers may benefit from these findings while formulating the curricula and instructions/regulations for ChatGPT use, as well as when designing the teaching methods and assessment tools. …”
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The impact of multiple exposures and movement on the fear response of poultry by Alexandra Jackson, Marcela Quino, Anusha Gautam, Melissa Gilpin, Katie Still, Denise Landers, Bethany Baker-Cook

“…This impact of repeated exposure is important to recognize when designing experiments that utilize fear tests.…”
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Molecular cloning and expression dynamics of Atrogin-1 in muscle tissue of Russian sturgeon (Acipenser gueldenstaedtii) under high salt stress by Yong-Juan Mo, Yi-Li Gao, Shi-Jjian Xu, Bin Wang, Jingping Wu, Peng Yu, Ming Li, Xiangtao Kang, Zhi-Qiang Han

“…Utilizing the sequence of Russian sturgeon atrogin-1, we designed novel primers and a specific anti-peptide antibody for our study, confirming that atrogin-1 is selectively expressed in skeletal muscle and heart tissues. …”
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Development of a recombinant infectious bronchitis virus vaccine expressing infectious laryngotracheitis virus multiple epitopes by Guanming Shao, Jun Fu, Yun Pan, Shiying Gong, Chaoyi Song, Sheng Chen, Keyu Feng, Xinheng Zhang, Qingmei Xie

“…Infectious bronchitis virus (IBV) is considered a potential vector for vaccine development, but the issue of poor stability in recombinant IBV expressing foreign genes has not yet been resolved. In this study, we designed a multi-epitope cassette (gD-T/B) containing multiple T and B cell epitopes of ILTV gD protein. …”
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The effectiveness and risks of Treating people with Idiopathic Pulmonary fibrosis with the Addition of Lansoprazole (TIPAL): study protocol for a randomised placebo-controlled mult... by Toby M Maher, A John Simpson, Anthony Cahn, David Thickett, Luke Vale, Allan B Clark, Nazia Chaudhuri, Ian Forrest, Lisa G Spencer, Christopher Ward, Stephen Jones, Ganesh Raghu, Andrew M Wilson, Helen Parfrey, Megan Jones, Matthew Hammond, Jaclyn Ann Smith, Shajahan Wahed

“…High-resolution CT scoring will be undertaken in a subgroup. The trial is designed to determine whether treating people with IPF with lansoprazole will reduce the reduction in FVC over a year. …”
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Patient participation in free cataract surgery: a cross-sectional study of the low-income elderly in urban China by Bo Qu, Hui Chen, Xuhua Tan, Xiaohang Wu, Haotian Lin, Zhenzhen Liu, Lixia Luo, Bo Zhang, Erping Long, Duoru Lin, Weirong Chen, Yizhi Liu, Haofeng Jiang, Jingjing Chen, Zhuoling Lin, Xiaoyan Li, Jinzhu Tang, Yingfen Lin

“…Objectives To explore the characteristics of the low-income elderly who underwent free cataract surgery and to determine the degree of patient satisfaction with the free cataract surgery programme in urban China.Methods A free cataract surgery management workflow was designed as a poverty relief project in Guangzhou. …”
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60-month follow-up of Long Limb vs. Standard Limb Roux-en-Y gastric bypass for type 2 diabetes and obesity: the LONG LIMB RCT by Saleem Ansari, Anna Kamocka, Tina Mazaheri, Ibiyemi Ilesanmi, Lara Jimenez-Pacheco, Kleopatra Alexiadou, Joanna Tan, Harvinder Chahal, Krishna Moorthy, Sanjay Purkayastha, Anne Margot Umpleby, Stephen Robert Bloom, Francesco Rubino, Alexander Dimitri Miras, Ahmed Rashid Ahmed, Tricia Tan

“…Limitations Although the surgical procedures were designed according to United Kingdom clinical practice at the time of study inception, there is substantial variation in practice internationally. …”
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Effects of nanocurcumin supplementation on metabolic syndrome: A systematic review and meta-analysis of randomized controlled trials by Gofarana Wilar, Cecep Suhandi, Kohji Fukunaga, Masanori Shigeno, Ichiro Kawahata, Rizky Abdulah, Takuya Sasaki

“…However, the variability in study participant ages, treatment durations, and sample sizes suggests the need for further well-designed RCTs to establish optimal usage guidelines.…”
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Lipopolymers as the Basis of Non-Viral Delivery of Therapeutic siRNA Nanoparticles in a Leukemia (MOLM-13) Model by Panadda Yotsomnuk, Amarnath Praphakar Rajendran, Daniel Nisakar Meenakshi Sundaram, Luis Carlos Morales, Cezary Kucharski, Mohammad Nasrullah, Wanwisa Skolpap, Xiaoyan Jiang, Spencer B. Gibson, Joseph Brandwein, Hasan Uludağ

“…These results demonstrate the potential of designed lipopolymers in implementing RNAi (via delivery of siRNA) for inhibition of leukemia growth and provide evidence for the feasibility of targeting different oncogenes using siRNA-mediated therapy.…”
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Bayesian joint-regression analysis of unbalanced series of on-farm trials by Turbet Delof, Michel, Rivière, Pierre, Dawson, Julie C, Gauffreteau, Arnaud, Goldringer, Isabelle, van Frank, Gaëlle, David, Olivier

“…This challenge is not unique to PPB, as many breeding programs use sparse testing or incomplete block designs to evaluate more genotypes, however in PPB genotypes are not always assigned randomly to environments. …”
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Relationship between bacteria and nitrogen dynamics in wastewater treatment oxidation ponds by S. Saneha, T. Pattamapitoon, S. Bualert, O. Phewnil, W. Wararam, N. Semvimol, K. Chunkao, C. Tudsanaton, M. Srichomphu, U. Nachaiboon, O. Wongsrikaew, P. Wichittrakarn, C. Chanthasoon

“…Oxidation ponds act as a natural treatment system for wastewater and are designed to promote the growth and activity of certain bacterial species that remove contaminants from the water. …”
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Substantia nigra alterations in mice modeling Parkinson’s disease by I. N. Rozhkova, S. V. Okotrub, E. Yu. Brusentsev, T. A. Rakhmanova, D. A. Lebedeva, V. S. Kozeneva, N. A. Shavshaeva, N. V. Khotskin, S. Ya. Amstislavsky

“…To study the mechanisms of this pathology, genetic models in mice have been designed. Transgenic mice of the B6.Cg-Tg(Prnp-SNCA*A53T)23Mkle/J strain (referred to as B6.Cg-Tg further in the text) possess the A53T mutation in the human alpha-synuclein SNCA gene. …”
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