Application of 2D polymerase chain reaction for single-tube detection of high-risk human papillomaviruses by Weifeng Wang, Min Jiang, Ying Liu, Xuan Wu, Yan Chen, Xiaoyun Zhang, Weiwei Liu

“…This research endeavored to develop a high-throughput 2D PCR method for closed-tube genotyping of 11 HR-HPVs.MethodsBase-quenched probes were specifically designed for FAM, VIC, and CY5 channels. The 2D PCR system underwent optimization, with its detection performance assessed in terms of specificity and sensitivity. …”
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Detection and characterization of bovine hepacivirus in cattle and sheep from Hulunbuir, northeastern China by Jingge Ma, Jingge Ma, Zhiwei Wei, Liang Li, Wei Wang, Ziyan Liu, Ziyan Liu, Ning Liu, Feng Wei, Xiangyu Zheng, Zedong Wang

“…BovHepV-positive samples were confirmed using semi-nested PCR with primers designed based on the obtained viral sequences. Comprehensive bioinformatics analyses were employed to assess sequence similarity, phylogenetic evolution, and recombination of the obtained viral strains.ResultsA total of 988 serum samples from sheep (520) and cattle (468) were collected from 12 administrative districts in Hulunbuir from June to August, 2022. …”
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Genetics of Stay-Green Trait and Its Association with Leaf Spot Tolerance and Pod Yield in Groundnut by Rukiya Danful, Yussif Baba Kassim, Doris Kanvenaa Puozaa, Richard Oteng-Frimpong, Masawudu Abdul Rasheed, Alexander Wireko-Kena, Richard Akromah

“…However, this relationship has not been explored in groundnut. This study was designed to determine the genetic control of the stay-green trait and its relationship with leaf spot disease severity in groundnut. …”
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Electroacupuncture and Tongbian decoction ameliorate CUMS-induced depression and constipation in mice via TPH2/5-HT pathway of the gut-brain axis by Yi Chen, Peihua Shen, Qi Li, Shun Seng Ong, Yunzhi Qian, Hai Lu, Ming Li, Tianshu Xu

“…Current treatments, such as antidepressants and prokinetics, are often associated with side effects and high recurrence rates, highlighting the need for effective treatments targeting both depression and constipation. This study was designed to assess the therapeutic efficacy of electroacupuncture (EA) in conjunction with Tongbian decoction (TB) for the management of both depression and constipation, while also investigating the underlying mechanisms through the lens of the gut-brain axis. …”
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Comprehensive dialectical behavior therapy for adolescents in a juvenile correctional treatment center: a pilot evaluation by Johanna B. Folk, Phillip Yang, Anne Thomas, Jayme Lyon, Jaisal Patel, Clara Yoon, Barbara Robles-Ramamurthy, Barbara Robles-Ramamurthy, Barbara Robles-Ramamurthy

“…As part of a facility-designed program evaluation, youth completed a battery of empirically validated assessments of mental health and emotion regulation prior to and following completion of the program.ResultsResults show that comprehensive DBT-A is feasible to implement in a juvenile correctional treatment center and overall, youth improved from pre- to post-treatment in mental health symptoms and emotion regulation, with small to medium effect sizes.ConclusionThese findings build upon a growing literature showing dialectical behavior therapy is a promising intervention for treating emotion dysregulation and mental health conditions and can be successfully implemented in juvenile forensic settings.…”
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A Lightweight Network for Ship Detection in SAR Images Based on Edge Feature Aware and Fusion by Yuming Li, Jin Liu, Xingye Li, Xiliang Zhang, Zhongdai Wu, Bing Han

“…Furthermore, a lightweight feature fusion network, Filter-Pruned Bi-directional Feature Pyramid Network (FP-BiFPN), is carefully designed, which can not only suppresses background information, but also accentuates ship targets. …”
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Development of a duplex real-time recombinase aided amplification assay for the simultaneous and rapid detection of PCV3 and PCV4 by Renjie Sun, Hanze Liu, Siqi Sun, Yating Wang, Ying Shan, Xiaoliang Li, Weihuan Fang, Yongle Yang, Ronghui Xie, Lingyan Zhao

“…The assay was completed within 20 min at 39℃ with the designed optimal primers and probes. Results The established assay was more convenient and simpler operation compared with conventional molecular biological assays. …”
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Genome analysis of Bifidobacterium adolescentis and investigation of its effects on inflammation and intestinal barrier function by Bo Li, Bo Li, Haoyu Wang, Haoyu Wang, Haoyu Wang, Mengmeng Wang, Mengmeng Wang, Hewei Liang, Hewei Liang, Hewei Liang, Tongyuan Hu, Tongyuan Hu, Tongyuan Hu, Jinlong Yang, Jinlong Yang, Jinlong Yang, Shangyong Li, Xinbi You, Xinbi You, Xinbi You, Binbin Xia, Binbin Xia, Binbin Xia, Yue Yuan, Yue Yuan, Yuanqiang Zou, Yuanqiang Zou, Yinglei Miao, Yinglei Miao, Yang Sun, Yang Sun, Yang Sun

“…Based on the bacterial collection constructed in our previous studies, we founded that Bifidobacterium adolescentis AF91-08b2A has the potential beneficial function. We designed cohort studies, genomic studies and animal experiments to further explore the probiotic function of Bifidobacterium adolescentis AF91-08b2A and its therapeutic effect on IBD. …”
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In vivo production of engineered ACE2 decoy protects lungs from SARS-CoV-2 infection by Yuta Suzuki, Takayuki Miyazaki, Yoko Ida, Tatsuya Suzuki, Yumi Itoh, Shuto Nakao, Keita Kondo, Kenji Kubara, Keisuke Nishioka, Hiroki Muto, Ryuji Watari, Toshifumi Hirayama, Dai Kakiuchi, Shinya Sato, Satoshi Inoue, Yoshifumi Uemoto, Yohei Mukai, Atsushi Hoshino, Toru Okamoto, Junji Matsui

“…Thus, next-generation therapeutics that are resistant to mutations and can be rapidly supplied to individuals in an emergency are required. Here, we designed an mRNA encoding an engineered angiotensin-converting enzyme 2 (ACE2) decoy, 3N39v4, composed of high-affinity ACE2 and a human immunoglobulin G Fc domain. …”
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In-vivo high-resolution χ-separation at 7T by Jiye Kim, Minjun Kim, Sooyeon Ji, Kyeongseon Min, Hwihun Jeong, Hyeong-Geol Shin, Chungseok Oh, Robert J. Fox, Ken E. Sakaie, Mark J. Lowe, Se-Hong Oh, Sina Straub, Seong-Gi Kim, Jongho Lee

“…To address these challenges, we developed a novel deep neural network, R2PRIMEnet7T, designed to convert a 7T R2* map into a 3T R2′ map. …”
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CCD Standard Curve Fitting for Microarray Detection Base on Multi-Layer Perceptron by Zhenhua Gan, Dongyu He, Peishu Wu, Baoping Xiong, Nianyin Zeng, Fumin Zou, Feng Guo, Qin Bao, Fengyan Zhao

“…Due to the characteristics of semiconductors, the CCD sensor in the scanner we designed suffers from saturation, the non-linear relationship between photoelectric response and the light intensity collected by CCD, which poses a challenge for fitting the standard curve of microarray scanner. …”
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Secretory mitophagy: an extracellular vesicle-mediated adaptive mechanism for cancer cell survival under oxidative stress by Purva V. Gade, Angela Victoria Rojas Rivera, Layla Hasanzadah, Sofie Strompf, Thomas Raymond Philipson, Matthew Gadziala, Atharva Tyagi, Arnav Bandam, Rithvik Gabbireddy, Fatah Kashanchi, Amanda Haymond, Lance A. Liotta, Marissa A. Howard

“…Ultimately, our findings may inform new prevention strategies targeting pre-malignant lesions and therapeutic approaches designed to sensitize tumor cells to oxidative stress-inducing therapies.…”
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The dipeptide carnosine alleviates acute pancreatitis in an experimental model by Heresh Rezaei, Saeed Heidari, Mohammad Mehdi Ommati, Babak Taheri, Forouzan Khodaei, Mohammad Ali Dehghani, Ayeh Rowhanirad, Azadeh Veisi-Goshtasb, Zahra Honarpishefard, Negar Azarpira, Hossein Niknahad, Reza Heidari

“…The current study was designed to evaluate the impact of carnosine in an experimental model of AP. …”
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Aberrant methylation of placental development genes in chorionic villi of spontaneous abortions with trisomy 16 by О. Yu. Vasilyeva, E. N. Tolmacheva, A. E. Dmitriev, Ya. A. Darkova, E. A. Sazhenova, T. V. Nikitina, I. N. Lebedev, S. A. Vasilyev

“…To obtain sequencing libraries, targeted amplification of individual gene regions using designed oligonucleot ide primers for bisulfite-converted DNA was used. …”
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Protective Role of Genistein in Acute Liver Damage Induced by Carbon Tetrachloride by Nalan Kuzu, Kerem Metin, Adile Ferda Dagli, Fatih Akdemir, Cemal Orhan, Mehmet Yalniz, Ibrahim Hanifi Ozercan, Kazim Sahin, Ibrahim Halil Bahcecioglu

“…The first group was designated as the control group (group 1). The second group was injected with intraperitoneal CCl4 for 3 days (group 2). …”
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Successful Use of Negative Pressure Wound Therapy for Abdominal Wall Necrosis Caused by a Perforated Ascending Colon Using the ABThera System by Kouki Imaoka, Takuya Yano, Yasuhiro Choda, Ko Oshita, Yuma Tani, Tetsushi Kubota, Michihiro Ishida, Daisuke Satoh, Masanori Yoshimitsu, Kanyu Nakano, Masao Harano, Hiroyoshi Matsukawa, Hitoshi Idani, Shigehiro Shiozaki, Masazumi Okajima

“…A new device, the ABThera™ Open Abdomen Negative Pressure Therapy System (KCI USA, San Antonio, TX, USA), was specifically designed to achieve a temporary abdominal closure (TAC) in the management of an OA. …”
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Performance of the AmpliSeq NGS panel in thyroid nodules with indeterminate cytology by Wiame Potonnier, Erell Guillerm, Claude Bigorgne, Cécile Ghander, Malanie Roy, Florence Coulet, François Ansart, Fabrice Menegaux, Laurence Leenhardt, Isabelle Brocheriou, Gabrielle Deniziaut, Camille Buffet

“…The final histological diagnosis was used as the designated gold standard. Results: Histologically, 86 nodules were benign and 35 nodules were malignant. …”
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<inline-formula><tex-math notation="LaTeX">$\mathrm{D}^{3}$</tex-math></inline-formula>T: Deep Denoising Dictionary Tensor for Hyperspectral Anomaly Detection by Qiangqiang Shen, Zonglin Liu, Hanzhang Wang, Yanhui Xu, Yongyong Chen, Yongsheng Liang

“…In particular, the proposed <inline-formula><tex-math notation="LaTeX">$\mathrm{D}^{3}$</tex-math></inline-formula>T first designs a tensor recovery module for dividing the low-rank background and sparse anomalies, where the background is optimized as a denoising dictionary tensor by the joint low-rankness and deep denoising. …”
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A lipidated peptide derived from the C-terminal tail of the vasopressin 2 receptor shows promise as a new β-arrestin inhibitor by Rebecca L. Brouillette, Christine E. Mona, Michael Desgagné, Malihe Hassanzedeh, Émile Breault, Frédérique Lussier, Karine Belleville, Jean-Michel Longpré, Michel Grandbois, Pierre-Luc Boudreault, Élie Besserer-Offroy, Philippe Sarret

“…To address this issue, we designed and synthesised a novel lipidated phosphomimetic peptide inhibitor targeting β-arrestins, called ARIP, which was developed based on the C-terminal tail (A343-S371) of the vasopressin V2 receptor. …”
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Exploring the need for fertility education: A cross-sectional study of healthcare professionals and students’ knowledge of fertility and fertility preservation by Ana GALHARDO, Catarina SALVADO, Soraia MONIZ, Marina Cunha, Ilda MASSANO-CARDOSO

“…These results highlight the relevance of designing training and information opportunities to enhance knowledge on the topics of fertility awareness and fertility preservation.…”
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