Fas Ligand DNA Enhances a Vaccination Effect by Coadministered DNA Encoding a Tumor Antigen through Augmenting Production of Antibody against the Tumor Antigen by Boya Zhong, Guangyu Ma, Ayako Sato, Osamu Shimozato, Hongdan Liu, Quanhai Li, Masato Shingyoji, Yuji Tada, Koichiro Tatsumi, Hideaki Shimada, Kenzo Hiroshima, Masatoshi Tagawa

“…Interaction of Fas and Fas ligand (FasL) plays an important role in the regulation of immune responses by inducing apoptosis of activated cells; however, a possible role of FasL in DNA vaccination has not been well understood. We examined whether administration of DNA encoding FasL gene enhanced antitumor effects in mice that were vaccinated with DNA expressing a putative tumor antigen gene, β-galactosidase (β-gal). …”
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Potential transmission test of GP25 vaccine in normal flora bacteria of common carp culture media by Sri Nuryati, , Alimuddin, Ayu Dhita Juliadiningtyas

“…One way to prevent the spread of KHV is  by applying DNA vaccine. This research was conducted to test potential transmission of DNA vaccine encoding glycoprotein 25 (vaccine GP25) toward normal flora bacteria of media cultivation of common carp. …”
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Kloning gen virulen Streptococcus agalactiae sebagai bahan dasar vaksin rekombinan by , Sutanti, Retno Damayanti Soejoedono, Irvan Faizal

“…The mga gene  controlled by MBA promoter has constructed successfully as a candidate of DNA vaccine to against S. agalactiae infection in Nile tilapia.   …”
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Developing an Effective Therapeutic HPV Vaccine to Eradicate Large Tumors by Genetically Fusing Xcl1 and Incorporating IL-9 as Molecular Adjuvants by Zhongjie Sun, Zhongyan Wu, Xuncheng Su

“…Methods: We genetically fused the chemokine XCL1 with the E6 and E7 proteins of HPV16 to target cDC1 and enhance the vaccine-induced cytotoxic T cell response, ultimately developing a DNA vaccine. Additionally, we screened various interleukins and identified IL-9 as an effective molecular adjuvant for our DNA vaccine. …”
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Immunogenicity of HIV-1 <i>Env</i> mRNA and <i>Env-Gag</i> VLP mRNA Vaccines in Mice by Qi Ma, Jing Yang, Xiaoguang Zhang, Hongxia Li, Yanzhe Hao, Xia Feng

“…BALB/c mice were immunized with env mRNA, env–gag VLP mRNA, env plasmid DNA vaccine, or lipid nanoparticle (LNP) controls. HIV Env-specific binding and neutralizing antibodies in mouse sera were assessed via enzyme-linked immunosorbent assay (ELISA) and pseudovirus-based neutralization assays, respectively. …”
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Intradermal DNA Electroporation Induces Cellular and Humoral Immune Response and Confers Protection against HER2/neu Tumor by Alessia Lamolinara, Lorenzo Stramucci, Albana Hysi, Manuela Iezzi, Cristina Marchini, Marianna Mariotti, Augusto Amici, Claudia Curcio

“…Skin represents an attractive target for DNA vaccine delivery because of its natural richness in APCs, whose targeting may potentiate the effect of vaccination. …”
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mRNA technology as one of the promising platforms for the SARS-CoV-2 vaccine development by A. A. Ilyichev, L. A. Orlova, S. V. Sharabrin, L. I. Karpenko

“…Almost all known approaches to vaccine design were applied for this purpose, including inactivated viruses, mRNA and DNA-vaccines, vaccines based on various viral vectors, synthetically generated peptides and recombinant proteins produced in cells of insects and mammals. …”
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Applying the Concept of Peptide Uniqueness to Anti-Polio Vaccination by Darja Kanduc, Candida Fasano, Giovanni Capone, Antonella Pesce Delfino, Michele Calabrò, Lorenzo Polimeno

“…As an additional advantage, using a peptide-based vaccine instead of current anti-polio DNA vaccines would eliminate the rare post-polio poliomyelitis cases and other disabling symptoms that may appear following vaccination.…”
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Modern vaccines and coronavirus infections by Yu. A. Belikova, Yu. V. Samsonov, E. V. Abakushina

“…This review is on recent advances in the development of new generation vaccines, as well as those developed using earlier time-tested technologies: live attenuated vaccines, inactivated vaccines, recombinant vaccines, subunit vaccines, virus-like particle-based vaccines, synthetic peptide vaccines, DNA vaccines and mRNA vaccines. However, many infections are still not preventable with the currently available vaccines and they represent a major cause of mortality worldwide — for example, severe acute respiratory syndrome coronavirus, middle east respiratory syndrome coronavirus and severe acute respiratory syndrome coronavirus 2 that causes coronavirus disease 2019 — COVID-19. …”
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IMMUNOPROPHYLAXIS OF CANINE PARVOVIRAL ENTERITIS by A. V. Shatalov, S. P. Dannikov, A. N. Kononov, V. S. Skripkin

“…The use of immunomodulators as adjuvants in vaccine composition is proved to be effective to maintain the high titre of antibodies against canine parvovirus type 2 in the post-vaccination period, and the modern DNA-vaccine is a reasonable alternative to conventional vaccination. …”
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Safety, Immunogenicity, and Efficacy of Cytomegalovirus Vaccines: A Systematic Review of Randomized Controlled Trials by Manuela Chiavarini, Anita Genga, Giorgia Maria Ricciotti, Marcello Mario D’Errico, Pamela Barbadoro

“…<b>Results</b>: Several vaccine candidates have shown encouraging results in terms of safety and specific immune responses, notably adjuvanted gB vaccines and DNA vaccines targeting gB and pp65. The results were divided into RCTs on healthy individuals and those on transplant recipients, because the CMV-specific immune response to a vaccine is complex and varies depending not only on the type of vaccine, but also on the immunological status of the individual. …”
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Fusion to Flaviviral Leader Peptide Targets HIV-1 Reverse Transcriptase for Secretion and Reduces Its Enzymatic Activity and Ability to Induce Oxidative Stress but Has No Major Eff... by Anastasia Latanova, Stefan Petkov, Yulia Kuzmenko, Athina Kilpeläinen, Alexander Ivanov, Olga Smirnova, Olga Krotova, Sergey Korolev, Jorma Hinkula, Vadim Karpov, Maria Isaguliants, Elizaveta Starodubova

“…Positive outcome was the abrogation of polymerase activity increasing safety of RT-based DNA vaccines. Identification of the molecular determinants of low cellular immunogenicity of RT requires further studies.…”
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Lipid nanoparticles deliver DNA-encoded biologics and induce potent protective immunity by Dafei Chai, Junhao Wang, Jing Ming Lim, Xiaohui Xie, Xinfang Yu, Dan Zhao, Perry Ayn Mayson Maza, Yifei Wang, Dana Cyril-Remirez, Ken H. Young, Yong Li

“…The LNP formulation used in Moderna’s Spikevax mRNA vaccine (LNP-M) demonstrated a stable nanoparticle structure, high expression efficiency, and low toxicity. Notably, a DNA vaccine encoding the spike protein, delivered via LNP-M, induced stronger antigen-specific antibody and T cell immune responses compared to electroporation. …”
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