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MsDpo4—a DinB Homolog from Mycobacterium smegmatis—Is an Error-Prone DNA Polymerase That Can Promote G:T and T:G Mismatches
Published 2012-01-01“…Error-prone DNA synthesis in prokaryotes imparts plasticity to the genome to allow for evolution in unfavorable environmental conditions, and this phenomenon is termed adaptive mutagenesis. …”
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22
Real-time analysis of initiation regulation systems during the progression of the reconstituted chromosomal replication cycle
Published 2025-02-01“…The oriC sequestration system involves SeqA binding to hemimethylated oriC—generated during nascent DNA synthesis to prevent reinitiation. SeqA inhibited RCR, but the addition of Dam methylase relieved this inhibition. …”
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23
Ultraviolet B irradiation-induced keratinocyte senescence and impaired development of 3D epidermal reconstruct
Published 2021-06-01“…Senescence induced by high dose UVB resulted in morphological changes, enhanced β-gal activity, elevated cellular ROS levels and reduced DNA synthesis. qPCR revealed differential expression of the genes regulated senescence. p16INK4a expression was significantly increased in NHK exposed to UVB whereas enhanced IFI27 expression was observed only in cultural senescence. …”
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24
Synthetic genomics in crop breeding: Evidence, opportunities and challenges
Published 2025-02-01“…Recent strides in DNA synthesis methodologies, exemplified by innovative techniques like SCRaMbLE, have empowered the assembly and engineering of viral and microbial genomes. …”
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25
ErbB4 as a Potential Molecular Target in the Treatment of Esophageal Squamous Cell Cancers
Published 2014-01-01“…Additionally, miRNA-140-5p had marked effect on the DNA synthesis and caspase 3/7 activity in comparison to control cells. …”
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26
Rev1, Rev3, or Rev7 siRNA Abolishes Ultraviolet Light-Induced Translesion Replication in HeLa Cells: A Comprehensive Study Using Alkaline Sucrose Density Gradient Sedimentation
Published 2010-01-01“…The replicative polymerase then resumes DNA synthesis. This process, termed translesion replication (TLS) or replicative bypass, may involve at least five different polymerases in mammals, although the participating polymerases and their roles have not been entirely characterized. …”
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27
Antihuman Immunodeficiency Virus Type 1 (HIV-1) Activity of Rare Earth Metal Complexes of 4-Hydroxycoumarins in Cell Culture
Published 2006-01-01“…Detection of endogenous reverse transcriptase (RT) activity and RT processivity by PCR indicative for proviral DNA synthesis demonstrated that anti-HIV activity has not been linked to early stages of viral replication. …”
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28
Antihuman Immunodeficiency Virus Type 1 (HIV-1) Activity of Rare Earth Metal Complexes of 4-Hydroxycoumarins in Cell Culture
Published 2006-01-01“…Detection of endogenous reverse transcriptase (RT) activity and RT processivity by PCR indicative for proviral DNA synthesis demonstrated that anti-HIV activity has not been linked to early stages of viral replication. …”
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29
Biofunctionalized patterned platform as microarray biochip to supervise delivery and expression of pDNA nanolipoplexes in stem cells via mechanotransduction
Published 2025-01-01“…Gene transfection efficiency was enhanced with increasing cell nanomechanics, which was also confirmed by the evaluation of cell internalization capacity of nanoparticles and DNA synthesis ability. This work will provide a new strategy to study functional biomaterials, microarray chips and internal mechanism of gene transfection in patterned stem cells on biochips. …”
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Single cell transcriptomics in blood of patients with chronic obstructive pulmonary disease
Published 2025-01-01“…The 10X Genomics Chromium Instrument and cDNA synthesis kit were utilized to generate a barcoded cDNA library for single cell RNA-sequencing. …”
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31
Pirfenidone Inhibits Hypoxic Pulmonary Hypertension through the NADPH/ROS/p38 Pathway in Adventitial Fibroblasts in the Pulmonary Artery
Published 2020-01-01“…Proliferation of HPAAFs was quantified by colorimetric 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) assays to assess cellular metabolic activity, cell counts, and ethynyldeoxyuridine (EdU) assays to detect DNA synthesis. Migration of HPAAFs was assessed by a wound healing assay. …”
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32
Association of HLA-B27 Gene and Rheumatoid Arthritis: Analysis of Potential Role as a Predictive Biomarker
Published 2024-10-01“…Samples of human blood from RA patients and healthy individuals were collected, and RNA extraction, cDNA synthesis and qPCR were carried out to detect relative expression of HLA-B27. …”
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Reassessing Nutritional Supplementation Program: The Impact of Maternal Vitamin B12 Levels on Feto-Maternal Outcomes
Published 2023-12-01“…Vit B12 is an important cofactor for enzymatic reactions related to DNA synthesis, folate, and cell metabolism. While folate deficiency has been acknowledged in not only the prevention of neural tube defects and other adverse pregnancy outcomes, the effects of low vit B12 status have not been well defined. …”
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34
Indoxyl Sulfate Induces Mesangial Cell Proliferation via the Induction of COX-2
Published 2016-01-01“…Following IS treatment, MCs exhibited increased total cell number, DNA synthesis rate, and number of cells in S and G2 phases paralleled with the upregulation of cyclin A2 and cyclin D1. …”
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35
Seed priming and abiotic stress tolerance in carrot: Unraveling the mechanisms of improved germination.
Published 2025-01-01“…Additionally, we investigated the impact of inhibitors (actinomycin D-inhibitor of transcription, cycloheximide-inhibitor of translation, hydroxyurea-inhibitor of DNA synthesis, cytochalasin-inhibitor of actin polymerization) on seed germination under stress conditions. …”
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36
A ribodepletion and tagging protocol to multiplex samples for RNA-seq based virus detection: application to the cassava virome
Published 2025-02-01“…Results Here, we develop a high-throughput sequencing protocol, named Ribo-M-Seq, that combines plant rRNA ribodepletion, cDNA synthesis, tagging with a 96 multiplexing scheme and Illumina sequencing. …”
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Synergic Interaction of Rifaximin and Mutaflor (Escherichia coli Nissle 1917) in the Treatment of Acetic Acid-Induced Colitis in Rats
Published 2016-01-01“…This effect was associated with significant reversion of the acetic acid-evoked decrease in mucosal blood flow and DNA synthesis. Moreover, administration of rifaximin significantly reduced concentration of proinflammatory TNF-α and activity of myeloperoxidase in colonic mucosa. …”
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Massively parallel homogeneous amplification of chip-scale DNA for DNA information storage (MPHAC-DIS)
Published 2025-01-01“…Abstract Chip scale DNA synthesis offers a high-throughput and cost-effective method for large-scale DNA-based information storage. …”
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Chronic Atrophic Gastritis Presenting as Hemolytic Anemia due to Severe Vitamin B12 Deficiency
Published 2021-01-01“…Vitamin B12 is an essential nutrient which plays an important role in neurological function, hematopoiesis, and DNA synthesis. Low levels usually stem from either poor intake or a malabsorptive process. …”
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An Update on African Trypanocide Pharmaceutics and Resistance.
Published 2023“…In AAT, quinapyramine sulfate interferes with DNA synthesis and suppression of cytoplasmic ribosomal activity in the mitochondria. …”
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