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141
Analysis of Bacterial Diversity in Different Heavy Oil Wells of a Reservoir in South Oman with Alkaline pH
Published 2018-01-01“…It can be concluded that the microbial community was different in all the 10 soil samples with Bacillus and Paenibacillus sp. as predominating genus. The 16S rDNA sequencing of cultivable spore-forming bacteria identified the hydrocarbon utilizing bacteria as Bacillus and Paenibacillus sp. and the nucleotide sequences were submitted to NCBI GenBank under accession numbers KP119097–KP119115. …”
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142
Novel PITX2 Mutations including a Mutation Causing an Unusual Ophthalmic Phenotype of Axenfeld-Rieger Syndrome
Published 2019-01-01“…All coding exons of PITX2 and FOXC1 were amplified by polymerase chain reaction (PCR) from genomic DNA and subjected to direct DNA sequencing. Analysis of mutations in control subjects was performed by heteroduplex single-strand conformation polymorphism (SSCP) analysis. …”
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143
Genetic Diversity and Structure of a Critically Endangered Ornamental Species, <i>Rhododendron farinosum</i>, with Extremely Small Populations
Published 2025-01-01“…To unravel the reasons behind the endangerment of this species and provide guidance for the rational conservation of this species, this study obtained a large number of SNP loci by using double-digest restriction-site-associated DNA sequencing (ddRAD-seq) to evaluate the genetic diversity and genetic structure of <i>R. farinosum</i>, as well as to infer the population history of this species. …”
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144
Prevalence and Characterization of Extended-Spectrum Beta-Lactamase-Producing Enterobacteriaceae Isolated in Canadian Hospitals: Results from CANWARD 2007
Published 2009-01-01“…Polymerase chain reaction and DNA sequencing were used to detect blaSHV, blaTEM, blaCTX-M and blaOXA-like genes. …”
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145
Prevalence of Multidrug Resistance and Extended-Spectrum β-Lactamase Carriage of Clinical Uropathogenic Escherichia coli Isolates in Riyadh, Saudi Arabia
Published 2018-01-01“…Detection of different ESBL variants was performed using DNA sequencing. Of 100 E. coli isolates, 67 were associated with multidrug resistance (MDR) phenotype. …”
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146
Fecal microbiota transplantation alleviates radiation enteritis by modulating gut microbiota and metabolite profiles
Published 2025-02-01“…Gut microbiota alterations and fecal metabolism were analyzed via 16S rDNA sequencing and targeted metabolomics. The results demonstrated that FMT, particularly in the M-FMT group, effectively alleviated RE by reducing DAI scores, histological damage, and inflammatory markers while enhancing enzyme activity, superoxide dismutase (SOD) levels, and intestinal absorption. …”
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147
Vaginal Microbiota Diversity of Patients with Embryonic Miscarriage by Using 16S rDNA High-Throughput Sequencing
Published 2020-01-01“…To further dissect the microbiota of the vagina in the two groups, 16S rDNA sequencing was performed. Bioinformatics analysis showed that 1096 and 998 overlapped operational taxonomic units were identified from the embryonic miscarriage and control groups, respectively. …”
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148
Genetic Variation of the DGAT1 gene in Dual-Purpose Dairy Cows and its Influence on Economically Important Breeding Traits
Published 2019-09-01“…MethodsDNA was extracted from hair samples, and the genotypes of the DGAT1 gene were determined using a combination of conventional PCR, restriction fragment length polymorphism (RFLP), gel electrophoresis, DNA sequencing and novel real-time PCR assays. ResultsThe three genotypes within the herd were: KK (n = 5), AA (n = 10), KA (n = 8). …”
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149
Molecular subtype of ovarian clear cell carcinoma: an analysis of 80 Chinese patients using the TCGA molecular classification of endometrial cancer
Published 2025-01-01“…Methods We performed DNA sequencing on 80 OCCC patients via a panel that contains 520 cancer-related genes. …”
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150
CaGe: A Web-Based Cancer Gene Annotation System for Cancer Genomics
Published 2012-03-01“…High-throughput genomic technologies (HGTs), including next-generation DNA sequencing (NGS), microarray, and serial analysis of gene expression (SAGE), have become effective experimental tools for cancer genomics to identify cancer-associated somatic genomic alterations and genes. …”
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151
Transplantation of fecal microbiota from low to high residual feed intake chickens: Impacts on RFI, microbial community and metabolites profiles
Published 2025-01-01“…Microbiota composition and functional profiles in LRFI and HFMT chickens formed a distinct cluster compared to HRFI chickens. Using 16S rDNA sequencing and RandomForest analysis, Slackia, Peptococcus, Blautia, and Dorea were identified as key microbial markers associated with feed efficiency. …”
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152
Endosperm culture-based allotriploid hybrid production from an interspecific cross of Haemanthus spp.: new insights into polyploidization and hybridization
Published 2025-02-01“…In addition, chloroplast DNA sequencing revealed maternal inheritance in the endosperm-derived plantlets, consistent with embryo-derived plantlets. …”
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153
Historical biogeography and systematics of yellow-bellied toads (Bombina variegata), with the description of a new subspecies from the Balkans
Published 2025-01-01“…In this study, we combine cytochrome b DNA barcoding data (1238 individuals from 355 localities), mitogenome phylogenetics (17.2 kb), gene-based nuclear phylogenetics (3.7 kb from four gene fragments) and multilocus phylogenomics (4759 loci / ~554 kb obtained by double digest Restriction Associated DNA sequencing; ddRAD-seq) to re-assess the diversification of B. variegata, and revisit its nomenclatural history to assign scientific names to phylogeographic lineages. …”
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154
Identification and genetic analysis of the recent Avian pox viruses from infected pigeons and chickens
Published 2025-01-01“…Results The polymerase chain reaction (PCR) assay was able to detect APV in all the tested samples; 12 positive samples (6 chicken flocks and 6 pigeon flocks) were selected for DNA sequencing. The sequences were submitted to GenBank with accession numbers OR027032 to OR027043. …”
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155
Cloning, expression, and purification of fusion antigens MPT83 and ESAT6 from the local strain of Mycobacterium tuberculosis in Escherichia coli as a seed vaccine candidate against...
Published 2025-03-01“…Following transformation into E. coli JM109, the plasmid was extracted, PCR amplified, and DNA sequencing. The existence of the appropriate recombinant Rv2873 + Rv3875 fusion genes was confirmed through the observation of a band of 948 base pairs in the colony PCR product containing fusion antigens. …”
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156
Dysbiosis of Gastric Mucosal Fungal Microbiota in the Gastric Cancer Microenvironment
Published 2022-01-01“…Gastric fungal microbiome profiling was performed with internal transcribed spacer (ITS) rDNA sequencing in primary tumor and corresponding paired normal mucosal tissues from 61 GC patients. …”
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157
Gut microbiota and quantitative traits divergence at different altitude of long-tailed dwarf hamsters, Cricetulus longicaudatus
Published 2025-01-01“…We examined the diversity, abundance, and community structure of the gut microbiota of long-tailed dwarf hamsters from eight regions in Shanxi province during summer using 16S rDNA sequencing technology and analyzed the relationships between these microbiota and environmental variables as well as morphological indicators. …”
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158
Screening of Indigenous Oxalate Degrading Lactic Acid Bacteria from Human Faeces and South Indian Fermented Foods: Assessment of Probiotic Potential
Published 2014-01-01“…These were identified as Lactobacillus fermentum and Lactobacillus salivarius using 16S rDNA sequencing. Three strains, Lactobacillus fermentum TY5, Lactobacillus fermentum AB1, and Lactobacillus salivarius AB11, exhibited good adhesion to HT-29 cells and strong antimicrobial activity. …”
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159
Pulmonary Bacterial Communities in Surgically Resected Noncystic Fibrosis Bronchiectasis Lungs Are Similar to Those in Cystic Fibrosis
Published 2012-01-01“…Bacterial 16S rRNA gene libraries were constructed from lung tissue of 10 non-CF bronchiectasis and 21 CF patients, followed by DNA sequencing of isolates from each library. Community characteristics were analyzed and compared between the two groups. …”
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160
Prevalence and Molecular Characterization of Cysticercus tenuicollis Isolated from Some Intermediate Host in Kurdistan-Iraq
Published 2018-09-01“…The selected cysts were preserved in 70% Ethanol for DNA extraction and molecular study, The mt-CO1 gene was amplified with a conventional polymerase chain reaction (PCR), the PCR product purified and DNA sequencing for reverse and forward strands was determined by a genetic analyzer, the obtained sequences aligned with the DNA sequences of T. hydatigena in Iran, Turkey and Palestine, which deposited in GenBank under the following accession number (JQ710588), (JN827307) and ( KM032284) respectively. …”
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