Diaporthaceae associated with root and crown rot of maize by S.C. Lamprecht, P.W. Crous, J.Z. Groenewald, Y.T. Tewoldemedhin, W.F.O. Marasas

“…Furthermore, to clarify the higher-level phylogeny of these fungal genera, isolates were subjected to DNA sequencing of the nuclear ribosomal DNA (ITS & LSU). …”
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The Diversity of Airborne Bacteria Over the Tibet Plateau Decreased by Taklimakan Dust by Fanli Xue, Zhongwei Huang, Xuefei Huo, Qingqing Dong, Zhengpeng Li, Qianqing Gu

“…In the summer of 2019, we simultaneously collected air samples from the Tibetan Plateau (Ali) and the Taklimakan Desert (Minfeng and Tazhong) and analyzed them using high‐throughput DNA sequencing techniques. We found that the diversity of airborne bacteria decreased by 42.2% during dust events. …”
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Vitamin D Receptor Gene Polymorphisms Influence T1D Susceptibility among Pakistanis by Maryam Mukhtar, Andleeb Batool, Abdul Wajid, Iram Qayyum

“…Genotypes were identified by DNA sequencing and PCR-RFLP. Results. The allelic and genotypic frequencies of FokΙ and ApaI were significantly associated with T1D (p<0.001) development. …”
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DNA alterations in ovarian adult granulosa cell tumours: A scoping review protocol. by Sven Karstensen, Karsten Kaiser, Caroline Moos, Tim Svenstrup Poulsen, Kirsten Jochumsen, Claus Høgdall, Finn Lauszus, Estrid Høgdall

“…<h4>Background</h4>Identifying and describing molecular alterations in tumors has become common with the development of high-throughput sequencing. However, DNA sequencing in rare tumors, such as ovarian adult granulosa cell tumor (aGCT), often lacks statistical power due to the limited number of cases in each study. …”
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Whole exome analysis of primary immunodeficiency by E. S. Rahmani, Н. Azarpara, M. Karimipoor, Н. Rahimi

“…However, thanks to advancing in the DNA sequencing method and availability of sophisticated sequencers molecular characterization of genetic disorders have been revolutionized. …”
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Development of a high-throughput screening system for identification of novel reagents regulating DNA damage in human dermal fibroblasts by Bae Seunghee, An In-Sook, An Sungkwan

“…In the second step, we validated certain effective reagents identified in the first step by analyzing the cell cycle, evaluating cell death, and performing HPRT-DNA sequencing in DREAM-F cells treated with these reagents and UVB. …”
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Singleton-based species names and fungal rarity: Does the number really matter? by Jonathan Cazabonne, Allison K. Walker, Jonathan Lesven, Danny Haelewaters

“…This number is expected to be refined, especially with increasing knowledge about microfungi in undersampled habitats and increasing amounts of data derived from environmental DNA sequencing. A significant proportion of newly generated sequences fail to match with already named species, and thus represent what has been referred to as fungal “dark taxa”. …”
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Apolipoprotein C-II Deposition Amyloidosis: A Potential Misdiagnosis as Light Chain Amyloidosis by Sadichhya Lohani, Emily Schuiteman, Lohit Garg, Dhiraj Yadav, Sami Zarouk

“…Further evaluation of biopsy specimens with laser microdissection and mass spectrometry and DNA sequencing of exons should be done routinely in patients with amyloidoses for definitive diagnosis. …”
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Chrysosporium queenslandicum: a potent keratinophilic fungus for keratinous waste degradation by Tarun Kumar Kumawat, Anima Sharma, Seema Bhadauria

“…This chicken feather degrading fungal strain previously isolated and identified by morphological and 18 s rDNA sequencing in laboratory, was used in the present study. …”
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Leishmania major Infection in Synanthropic Rodents: Evidence for the Urbanization of Zoonotic Cutaneous Leishmaniasis (ZCL) in Southern Iran by Saeed Shahabi, Kourosh Azizi, Qasem Asgari, Bahador Sarkari

“…Leishmania DNA was detected in 18 (43.9%) rodents, including 11 R. rattus, 6 M. musculus, and one M. persicus, based on DNA sequencing of the ITS2 gene and PCR of the kDNA. Phylogenetic reconstruction revealed that the parasite infecting the rodents was L. major. …”
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Interleukin-16 Polymorphism Is Associated with an Increased Risk of Ischemic Stroke by Xiao-li Liu, Jian-zong Du, Yu-miao Zhou, Qin-fen Shu, Ya-guo Li

“…A total of 198 patients with ischemic stroke and 236 controls were genotyped using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and DNA sequencing method. We found that the rs11556218TG genotype and G allele of IL-16 were associated with significantly increased risks of ischemic stroke (TG versus TT, adjusted OR = 1.88; 95% CI, 1.15–3.07; G versus T, adjusted OR = 1.54; 95% CI, 1.05–2.27, resp.). …”
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Isolation and identification of fungi found in contaminated fermented milk and antifungal activity of vanillin by Xu Pei, Mekonen Tekliye, Mingsheng Dong

“…Fungal isolates obtained were characterized by DNA sequencing of the internal transcribed spacer (ITS) region. …”
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THE BIOTECHNOLOGICAL POTENTIAL OF THE NEW CROP, Miscanthus cv . SORANOVSKII by N. M. Slynko, T. N. Goryachkovskaya, S. V. Shekhovtsov, S. V. Bannikova, N. V. Burmakina, K. V. Starostin, A. S. Rozanov, N. N. Nechiporenko, S. G. Veprev, V. K. Shumny, N. A. Kolchanov, S. E. Peltek

“…On the base of phenotyping and DNA sequencing, the new crop was identified as Miscanthus sacchariflorus. …”
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Reanalysis of sequences of alleged Javan tiger highlights the difficulties in studying big cats and the need for high-throughput sequencing by Anubhab Khan, Yulianto Yulianto, Sabhrina Gita Aninta, Wirdateti Wirdateti

“…However, there are several difficulties in using genetic tools for big cat conservation that could be resolved by modern methods of DNA sequencing. Recent reports of the sighting of a putative Javan tiger Panthera tigris sondaica in West Java, Indonesia, highlight some of the difficulties of studying the genetics of big cats. …”
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Modern approaches to artificial gene synthesis: aspects of oligonucleotide synthesis, enzymatic assembly, sequence verification and error correction by G. Y. Shevelev, D. V. Pyshnyi

“…Thus, significant reduction in cost of DNA sequencing has provided an easy access to large amounts of data on the genetic sequences of various organisms, and decreased the price of the DNA sequence synthesis, which, analogous to Moore’s law, resulted in an opportunity to create a lot of potential genes without the time – consuming and labor – intensive traditional methods of molecular biology. …”
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Comparative analysis of microbial contamination in diesel fuels using MALDI-TOF MS by Agnieszka Ludwiczak, Tomasz Zieliński, Ewelina Sibińska, Grażyna Czeszewska-Rosiak, Michał Złoch, Joanna Rudnicka, Andrzej Tretyn, Paweł Pomastowski

“…The Zybio system identified 48% of all microorganisms at the species level, whereas the Bruker system identified only 33%. The 16 S rDNA sequencing confirmed proteomic identifications by the MALDI systems with high accuracy. …”
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Molecular, Biochemical, and Clinical Characterization of Thirteen Patients with Glycogen Storage Disease 1a in Malaysia by Siti Aishah Abdul Wahab, Yusnita Yakob, Mohd Khairul Nizam Mohd Khalid, Noraishah Ali, Huey Yin Leong, Lock Hock Ngu

“…The G6PC mutation analysis was performed by PCR-DNA sequencing. Results. Patients were presented with hepatomegaly (92%), hypoglycaemia (38%), poor weight gain (23%), and short stature (15%). …”
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<i>TFL1</i>-like genes in <i>Vigna unguiculata</i> (L.) Walp. with different growth habit types by E. A. Krylova, E. K. Khlestkina

“…Using the Sanger DNA sequencing method, the primary structure of TFL1-like genes was studied in six cowpea accessions with different growth habit types and architectonics.Results. …”
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COEX-Seq: Convert a Variety of Measurements of Gene Expression in RNA-Seq by Sang Cheol Kim, Donghyeon Yu, Seong Beom Cho

“…Next generation sequencing (NGS), a high-throughput DNA sequencing technology, is widely used for molecular biological studies. …”
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Genetic Characterization of Escherichia coli O157 Isolated From Human Stool Specimens in Wassit Province of Iraq by Sabah M. Jwied, Mohammed Sh. Jebur

“…While 9 cases were positive for stx genes and have rfb gene. DNA sequences that depend on the sequence of the vtx2 gene have shown a highly homologous sequencing identity with NCBI-Blast Escherichia coli strain( O157:H7) isolates from humans and animals. …”
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