Protocols for translocation processes of flexible polymers through a pore using LAMMPS by Vrinda Garg, Rejoy Mathew, Arindam Chatterjee, Surya K. Ghosh

“…This protocol has potential applications in developing mathematical models for DNA sequencing, controlled drug delivery, and cellular transport processes.For complete details on the use and execution of this protocol, please refer to Garg et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
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The evolution of personalized medicine: literature review by I. S. Dolgopolov, M. Yu. Rykov

“…This belief was to somehow confirmed by the use of the latest technologies, such as DNA sequencing, proteomics, imaging protocols and the use of wireless devices for health monitoring, which revealed inter-individual differences in gene expression and penetrance levels. …”
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A Novel Method for Functional Annotation Prediction Based on Combination of Classification Methods by Jaehee Jung, Heung Ki Lee, Gangman Yi

“…NGS is a popular research method since high-throughput technologies such as DNA sequencing and microarrays have created large sets of genes. …”
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Investigation and Identification of Fungal Diseases of <i>Aloe barbadensis</i> in China by Guohui Zhang, Qingjia Wan, Xiangyang Li, Jie Deng

“…The pathogenic fungi of wild and cultivated species of <i>A. barbadensis</i> were isolated by a tissue separation method, and DNA sequencing was carried out by using the sequence analysis of the ribosomal rDNA-ITS region, and the pathogenic fungi were classified and identified by finally combining morphological observations. …”
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De Novo Mutation of m.3243A>G together with m.16093T>C Associated with Atypical Clinical Features in a Pedigree with MIDD Syndrome by Zhixin Jiang, Yinan Zhang, Jingbin Yan, Fengwen Li, Xinqian Geng, Huijuan Lu, Xiaoer Wei, Yanmei Feng, Congrong Wang, Weiping Jia

“…Clinical evaluations including endocrinological, audiological, and magnetic resonance imaging (MRI) examinations, mitochondrial function evaluation in peripheral blood mononuclear cells (PBMCs), and whole mitochondrial DNA (mtDNA) sequencing were performed among the spontaneous mutant pedigrees. …”
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Practical Tips for Construction of Custom Peptide Libraries and Affinity Selection by Using Commercially Available Phage Display Cloning Systems by Keisuke Fukunaga, Masumi Taki

“…In both systems, peptides containing odd number(s) of Cys should be designed with caution. Also, DNA sequencing of a constructed library before biopanning is highly recommended for finding unexpected bias.…”
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New Hosts of Simplicimonas similis and Trichomitus batrachorum Identified by 18S Ribosomal RNA Gene Sequences by Kris Genelyn B. Dimasuay, Orlie John Y. Lavilla, Windell L. Rivera

“…Genomic DNA was extracted using Chelex method and the 18S rRNA gene was successfully amplified through novel sets of primers and undergone DNA sequencing. Aligned isolate sequences together with retrieved 18S rRNA gene sequences of known trichomonads were utilized to generate phylogenetic trees using maximum likelihood and neighbor-joining analyses. …”
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Is the Goat a New Host for the G3 Indian Buffalo Strain of Echinococcus granulosus? by Pietro Calderini, Simona Gabrielli, Gabriella Cancrini

“…Specific PCR followed by DNA sequencing of the mitochondrial cox1 gene revealed for all the isolates 99.5% identity to the reference strain G3 genotype and 99.3% and 99.1% to G2 and G1, respectively. …”
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Rare Suprasellar Chordoid Meningioma with INI1 Gene Mutation by Imad Fadl-Elmula, Rania Giha, Sawsan AH Deaf, Lamyaa Ahmed El Hassan, Ahmed M. Hassan, Mohamed AR Arbab

“…Genomic DNA was extracted and mutation analysis for INI1 gene using primer of exon 4, 5, 7, and 9 showed mutation involving exon 9. DNA sequencing showedheterozygosity C­­­­­­­­­­­­­­­­­­-T mutation in exon 9 of INI1 gene leading to change of amino acid serine to phenylalanine at (codon 63). …”
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Detection of new single nucleotide polymorphisms in plasma cells carcinoma (myeloma) by polymerase chain reaction–single strand conformation polymorphism and sequencing technical m... by Sarah K. Obayes, Sabah H. Enayah, Shaimaa A. Al-Oubaidy, Rana A. Ghaleb

“…Genotyping of miRNA was accomplished using the polymorphism techniques of polymerase chain reaction and single strand conformation polymorphism (SSCP). DNA sequencing was also applied to categorize all the ranges of SSCP models detected using only gel imaging. …”
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Fatigue and Exercise Intolerance as Initial Manifestations of a Nonsyndromic Mitochondrial Disorder Due to the Variant m.3243A>G by Josef Finsterer, Sinda Zarrouk

“…Workup for suspected MID revealed ragged-red fibers and NADH-deficient muscle fibers, and biochemical investigations revealed a mild complex-I defect. mtDNA sequencing revealed the variant m.3243A>G with a heteroplasmy rate of 70% in the muscle. …”
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Morphological and Molecular studies of Acanthobra marmid Heckel, 1843 (Piscies, Cypriniformes, Leuciscidae) from the Middle of Iraq by Hind Dyia Hadi, Abed Hassan Baraaj, Atheer Hussein Ali

“…The barcoding result (DNA sequencing) in fishes found in the same family  (Leuciscidae) showed that it fit with A. marmid. …”
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Single‐egg comet assay: A protocol to quantify DNA damage in natural bioarchives by Rejin Salimraj, Alessio Perotti, Marcin W. Wojewodzic, Dagmar Frisch

“…We optimised the CA protocol for individual dormant propagules (single‐egg comet assay or SE‐CA) to inform downstream analyses such as DNA sequencing of the DNA quality contained in natural genetic archives of past populations. …”
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Environmental gradients shape genetic variation in the desert moss, Syntrichia caninervis Mitt. (Pottiaceae) by Ugbad A. Farah, Kirsten M. Fisher

“…Here, we sampled S. caninervis from sites along two elevation gradients and used restriction site associated DNA sequencing to compare the relative impacts of environmental factors and geospatial distances on genetic differentiation in S. caninervis populations. …”
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Isolation of Lodderomyces elongisporus from the Catheter Tip of a Fungemia Patient in the Middle East by Suhail Ahmad, Zia U. Khan, Molly Johny, Najat M. Ashour, Wehad H. Al-Tourah, Leena Joseph, Rachel Chandy

“…The identity of the isolate was confirmed by phenotypic characteristics and ribosomal DNA sequencing.…”
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Gene Mutation and Its Association with Clinicopathological Features in Young Patients with Non-Small-Cell Lung Cancer by Wencui Kong, Zongyang Yu, Wenwu Wang, Jingrong Yang, Jingfang Wang, Zhongquan Zhao

“…Clinicopathologic information of 102 young NSCLC patients was collected. Direct ctDNA sequencing of a portion of these patients was performed. …”
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Nocardia thailandica Brain Abscess in an Immunocompromised Patient by Muhammad Effendi, Samad Tirmizi, Dayna McManus, Anita J. Huttner, David R. Peaper, Jeffrey E. Topal

“…Upon identification by 16S rDNA sequencing, the organism causing infection was identified to be N. thailandica. …”
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Progression from Sustained BK Viruria to Sustained BK Viremia with Immunosuppression Reduction Is Not Associated with Changes in the Noncoding Control Region of the BK Virus Genome by Imran A. Memon, Bijal A. Parikh, Monique Gaudreault-Keener, Rebecca Skelton, Gregory A. Storch, Daniel C. Brennan

“…The antimetabolite was withdrawn for BK viremia and reduction of the calcineurin inhibitor for sustained BK viremia. DNA sequencing from the 11 patients with sustained viremia revealed 8 insertions, 16 transversions, 3 deletions, and 17 transitions. …”
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Fitness landscapes of human microsatellites. by Ryan J Haasl, Bret A Payseur

“…Advances in DNA sequencing technology and computation now enable genome-wide scans for natural selection to be conducted on unprecedented scales. …”
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p53 Mutations and DNA Ploidy in Colorectal Adenocarcinomas by Paola Campomenosi, Paola Assereto, Massimo Bogliolo, Gilberto Fronza, Angelo Abbondandolo, Antonella Capasso, Patrizia F. Bellomo, Roberto Monaco, Anna Rapallo, Andrea Sciutto, Roberto Orecchia, Elio Geido, Walter Giaretti

“…Analysis of p53 mutations spectrum of the sorted nuclei was done by Denaturing Gradient Gel Electrophoresis (DGGE) and DNA sequencing. Overall, we studied 20 adenocarcinomas, the corresponding control mucosa, and 7 lymph node metastases.…”
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