Molecular characterization, chemical profile and biological properties of essential oils from Chamaemelum nobile (L.) flowers of Morocco: in vitro and in silico studies by El-Mehdi El-Assri, El-Mehdi El-Assri, Youssef El-Assri, Rajae El Brahimi, Mohamed El fadili, Asmae Baghouz, Hatem A. Abuelizz, Sara Er-Rahmani, Amal Lahkimi, Abdelhak Bouia

“…Phylogenetic analysis of the DNA sequence revealed a 99.22% similarity with Chamaemelum nobile (L.).ConclusionThese results demonstrate the significant potential of Moroccan CN-EO in phytomedicine. …”
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Epigenetic modifications and emerging therapeutic targets in cardiovascular aging and diseases by Yurou Qiu, Qing Xu, Peichen Xie, Chenshuang He, Qiuchan Li, Xin Yao, Yang Mao, Xiaoqian Wu, Tiejun Zhang

“…Epigenetics, which modulates gene expression without DNA sequence changes, is shedding light on these mechanisms and their heritable effects. …”
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Structure and Expression of Two Sex-Related dmrt Genes in Oxyeleotris lineolata by Jiajia FAN, Dongmei MA, Huaping ZHU, Minghui LIN, Zaixuan ZHONG, Yuanyuan TIAN

“…In this study, the Oxldmrt1 and Oxldmrt3 cDNA sequence was obtained from gonad transcriptome of O. lineolata, identified using PCR. …”
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Identification and expression analysis of the heat shock proteins Hsp70, Hsp90, and Hsp90b in Litopenaeus vannamei under low-temperature stress by Min Peng, Hui Wang, Shuiping Wen, Zheng Liang, Zheng Huang, Bin Zhang, Tianchong Chen, Qingyun Liu, Qiangyong Li, Yuan Meng, Yuliu Huang, Chunling Yang, Digang Zeng, Manyuan Li, Weilin Zhu, Yongzhen Zhao

“…Additionally, the survival rate of L. vannamei significantly decreased following hsp90 knockdown. DNA sequence alignment identified 12 single nucleotide polymorphisms (SNPs) in the hsp70 gene and 17 SNPs in the hsp90 gene. …”
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Hepatitis B Virus-KMT2B Integration Drives Hepatic Oncogenic Processes in a Human Gene-edited Induced Pluripotent Stem Cells-derived ModelSummary by Jun Tsuchiya, Masato Miyoshi, Sei Kakinuma, Fukiko Kawai-Kitahata, Akihide Kamiya, Taro Shimizu, Ayako Sato, Keiya Watakabe, Tomohiro Mochida, Kento Inada, Rion Kamimae, Shun Kaneko, Miyako Murakawa, Sayuri Nitta, Mina Nakagawa, Mamoru Watanabe, Yasuhiro Asahina, Ryuichi Okamoto

“…Methods: Based on the genetic information on HBV-KMT2B integration in HCC, we determined its complete DNA sequence and transcript variants. To exclude the effect of other oncogenic mutations, we reproduced HBV integration in healthy donor iPSCs with an intact genome and analyzed its effects using iPSC-derived hepatic progenitor cells (HPCs) and hepatocytes (iPS-Heps). …”
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A Comparative Study on Phenotypic versus ITS-Based Molecular Identification of Dermatophytes Isolated in Dakar, Senegal by Khadim Diongue, Ludivine Bréchard, Mamadou Alpha Diallo, Mame Cheikh Seck, Mouhamadou Ndiaye, Aida Sadikh Badiane, Stéphane Ranque, Daouda Ndiaye

“…For comparison, ITS-based PCR and DNA sequencing were applied for identification of the isolated dermatophytes. …”
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Genomic Analysis Methods of Microorganisms by Canan Kebabçıoğlu, Osman Erganiş

“…MLVA determines the genetic relationships of bacterial strains and biovar-level differences and assesses the copy number of repeated DNA sequences. Sequencing provides genetic data by identifying DNA sequences; Sanger sequencing is the basis for next-generation approaches. …”
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Three new species of Mesobiotus (Eutardigrada: Macrobiotidae) from Sweden with an updated phylogeny of the genus by Sarah Atherton, Jens Hulterström, Roberto Guidetti, K. Ingemar Jönsson

“…All three species are distinguished morphologically and through differences in DNA sequences as supported by PTP and mPTP analyses. …”
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Clinical Diagnosis of X-Linked Spondyloepiphyseal Dysplasia Tarda and a Novel Missense Mutation in the Sedlin Gene (SEDL) by Lei Kong, Dongxu Wang, Shanshan Li, Chengsheng Zhang, Xiuyun Jiang, Qingbo Guan, Zhenlin Zhang, Fei Jing, Jin Xu

“…We extracted genomic DNA from five members of a four-generation Chinese SEDT kindred with three affected males and then analyzed the genetic mutation by PCR and DNA sequencing. Results. DNA sequencing showed that the genetic missense mutation occurred one bp upstream of exon 6 in the SEDL gene in two families, and a heterozygous mutation was found in a female carrier. …”
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From Genetics to Genomics of Epilepsy by Silvio Garofalo, Marisa Cornacchione, Alfonso Di Costanzo

“…The introduction of DNA microarrays and DNA sequencing technologies in medical genetics and diagnostics has been a challenge that has significantly transformed medical practice and patient management. …”
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How to describe a new fungal species by K.A. Seifert, A.Y. Rossman

“…The formal requirements and best practices for the publication of descriptions of new fungal species are discussed. Expectations for DNA sequences and cultures are considered. A model manuscript offers one possible approach to writing such a paper.…”
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Proposals for consideration at IMC12 to modify provisions related solely to fungi in Chapter F of the International Code of Nomenclature for algae, fungi, and plants by Tom W. May, David L. Hawksworth

“…The topics addressed relate to: fungi whose morph-names have the same epithet; the listing of synonyms under entries for protected names in the Code Appendices; the processes of protection and rejection; the use of DNA sequences as nomenclatural types; the use of genomes as nomenclatural types; and the designation of fungi known only from DNA sequences. …”
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Structural Determinants of Photoreactivity of Triplex Forming Oligonucleotides Conjugated to Psoralens by Rajagopal Krishnan, Dennis H. Oh

“…Triplex-forming oligonucleotides (TFOs) with both DNA and 2′-O-methyl RNA backbones can direct psoralen photoadducts to specific DNA sequences. However, the functional consequences of these differing structures on psoralen photoreactivity are unknown. …”
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Prevalence and Molecular Characterization of Cysticercus tenuicollis Isolated from Some Intermediate Host in Kurdistan-Iraq by Abdullah Ahmed Hama, Rostam Hama Zorab, Fatima Mohammed Ali, Awaz M. Salih, Amer Abdullah Hassan

“…The selected cysts were preserved in 70% Ethanol for DNA extraction and molecular study,  The mt-CO1 gene was amplified with a conventional polymerase chain reaction (PCR), the PCR product purified and DNA sequencing for reverse and forward strands was determined by a genetic analyzer, the obtained sequences aligned with the DNA sequences of T. hydatigena in Iran, Turkey and Palestine, which deposited in GenBank under the following accession number (JQ710588), (JN827307) and ( KM032284) respectively. …”
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Isolation of Lodderomyces elongisporus from the Catheter Tip of a Fungemia Patient in the Middle East by Suhail Ahmad, Zia U. Khan, Molly Johny, Najat M. Ashour, Wehad H. Al-Tourah, Leena Joseph, Rachel Chandy

“…The identity of the isolate was confirmed by phenotypic characteristics and ribosomal DNA sequencing.…”
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BAC-browser: the tool for synthetic biology by Tatiana A. Semashko, Gleb Y. Fisunov, Georgiy Y. Shevelev, Vadim M. Govorun

“…Its main tasks, such as the design of synthetic sequences and the assembly of DNA sequences from synthetic oligonucleotides, require specialized software. …”
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Specific PCR Primers Based on Cytochrome-b Gene for Molecular Detection of Javan Mongoose (Herpestes javanicus) by Sena Adi Subrata, Subeno Subeno, Atus Syahbudin

“…The design starts with selecting specific marker from 51 DNA sequences of cytochrome-b. The DNA sequences were of 19 Javan carnivore collected from GenBank (NCBI). …”
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Next-Generation Sequencing Analysis on Image-Guided Biopsy Samples in Early-Stage Lung Cancer: Feasibility Study and Comparison With Surgical Samples by Louis Gros, MD, Rowena Yip, PhD, MPH, Arel Golombeck, MD, David F. Yankelevitz, MD, Claudia I. Henschke, PhD, MD

“…We aimed to compare NGS success rates across biopsy techniques for these tumors, assess DNA sequencing quality, and verify reliability against surgical resection results. …”
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Molecular Identification of Fungal Communities in a Soil Cultivated with Vegetables and Soil Suppressiveness to Rhizoctonia solani by Silvana Pompéia Val-Moraes, Eliamar Aparecida Nascimbem Pedrinho, Eliana Gertrudes Macedo Lemos, Lucia Maria Carareto-Alves

“…This metagenomic DNA was used as a template to amplify fungal 18S rDNA sequences, and libraries were constructed in Escherichia coli by cloning PCR products. …”
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The Correlation between Gut Microbiota and Serum Metabolomic in Elderly Patients with Chronic Heart Failure by Zhenhua Wang, Zhaoling Cai, Markus W. Ferrari, Yilong Liu, Chengyi Li, Tianzhang Zhang, Guorong Lyu

“…The expression of inflammatory factors in blood was detected by ELISA. 16S rDNA sequencing was used to analyze the changes in microorganisms in the samples. …”
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