Novel primers improve species delimitation in Cercospora by Mounes Bakhshi, Mahdi Arzanlou, Asadollah Babai-ahari, Johannes Z. Groenewald, Pedro W. Crous

“…Recent studies have revealed multi-gene DNA sequence data to be highly informative for species identification in Cercospora, especially when used in a concatenated alignment. …”
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Human-genome single nucleotide polymorphisms affecting transcription factor binding and their role in pathogenesis by E. V. Antontseva, A. O. Degtyareva, E. E. Korbolina, I. S. Damarov, T. I. Merkulova

“…It should be noted that the presence of an association does not allow one to identify a functional, truly disease-associated DNA sequence variant among multiple marker SNPs that are detected due to linkage disequilibrium. …”
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Epigenetic regulation in coronary artery disease: from mechanisms to emerging therapies by Rui Gao, Rui Gao, Rui Gao, Meilin Liu, Meilin Liu, Meilin Liu, Haoyi Yang, Haoyi Yang, Haoyi Yang, Yuhan Shen, Yuhan Shen, Yuhan Shen, Ni Xia, Ni Xia, Ni Xia

“…Epigenetics is a field investigating heritable alterations in gene expression without DNA sequence changes, such as DNA methylation, histone modifications, and non-coding RNAs. …”
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Molecular characterization, chemical profile and biological properties of essential oils from Chamaemelum nobile (L.) flowers of Morocco: in vitro and in silico studies by El-Mehdi El-Assri, El-Mehdi El-Assri, Youssef El-Assri, Rajae El Brahimi, Mohamed El fadili, Asmae Baghouz, Hatem A. Abuelizz, Sara Er-Rahmani, Amal Lahkimi, Abdelhak Bouia

“…Phylogenetic analysis of the DNA sequence revealed a 99.22% similarity with Chamaemelum nobile (L.).ConclusionThese results demonstrate the significant potential of Moroccan CN-EO in phytomedicine. …”
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Identification and expression analysis of the heat shock proteins Hsp70, Hsp90, and Hsp90b in Litopenaeus vannamei under low-temperature stress by Min Peng, Hui Wang, Shuiping Wen, Zheng Liang, Zheng Huang, Bin Zhang, Tianchong Chen, Qingyun Liu, Qiangyong Li, Yuan Meng, Yuliu Huang, Chunling Yang, Digang Zeng, Manyuan Li, Weilin Zhu, Yongzhen Zhao

“…Additionally, the survival rate of L. vannamei significantly decreased following hsp90 knockdown. DNA sequence alignment identified 12 single nucleotide polymorphisms (SNPs) in the hsp70 gene and 17 SNPs in the hsp90 gene. …”
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A Comparative Study on Phenotypic versus ITS-Based Molecular Identification of Dermatophytes Isolated in Dakar, Senegal by Khadim Diongue, Ludivine Bréchard, Mamadou Alpha Diallo, Mame Cheikh Seck, Mouhamadou Ndiaye, Aida Sadikh Badiane, Stéphane Ranque, Daouda Ndiaye

“…For comparison, ITS-based PCR and DNA sequencing were applied for identification of the isolated dermatophytes. …”
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Genomic Analysis Methods of Microorganisms by Canan Kebabçıoğlu, Osman Erganiş

“…MLVA determines the genetic relationships of bacterial strains and biovar-level differences and assesses the copy number of repeated DNA sequences. Sequencing provides genetic data by identifying DNA sequences; Sanger sequencing is the basis for next-generation approaches. …”
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Three new species of Mesobiotus (Eutardigrada: Macrobiotidae) from Sweden with an updated phylogeny of the genus by Sarah Atherton, Jens Hulterström, Roberto Guidetti, K. Ingemar Jönsson

“…All three species are distinguished morphologically and through differences in DNA sequences as supported by PTP and mPTP analyses. …”
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Clinical Diagnosis of X-Linked Spondyloepiphyseal Dysplasia Tarda and a Novel Missense Mutation in the Sedlin Gene (SEDL) by Lei Kong, Dongxu Wang, Shanshan Li, Chengsheng Zhang, Xiuyun Jiang, Qingbo Guan, Zhenlin Zhang, Fei Jing, Jin Xu

“…We extracted genomic DNA from five members of a four-generation Chinese SEDT kindred with three affected males and then analyzed the genetic mutation by PCR and DNA sequencing. Results. DNA sequencing showed that the genetic missense mutation occurred one bp upstream of exon 6 in the SEDL gene in two families, and a heterozygous mutation was found in a female carrier. …”
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From Genetics to Genomics of Epilepsy by Silvio Garofalo, Marisa Cornacchione, Alfonso Di Costanzo

“…The introduction of DNA microarrays and DNA sequencing technologies in medical genetics and diagnostics has been a challenge that has significantly transformed medical practice and patient management. …”
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Proposals for consideration at IMC12 to modify provisions related solely to fungi in Chapter F of the International Code of Nomenclature for algae, fungi, and plants by Tom W. May, David L. Hawksworth

“…The topics addressed relate to: fungi whose morph-names have the same epithet; the listing of synonyms under entries for protected names in the Code Appendices; the processes of protection and rejection; the use of DNA sequences as nomenclatural types; the use of genomes as nomenclatural types; and the designation of fungi known only from DNA sequences. …”
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How to describe a new fungal species by K.A. Seifert, A.Y. Rossman

“…The formal requirements and best practices for the publication of descriptions of new fungal species are discussed. Expectations for DNA sequences and cultures are considered. A model manuscript offers one possible approach to writing such a paper.…”
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Structural Determinants of Photoreactivity of Triplex Forming Oligonucleotides Conjugated to Psoralens by Rajagopal Krishnan, Dennis H. Oh

“…Triplex-forming oligonucleotides (TFOs) with both DNA and 2′-O-methyl RNA backbones can direct psoralen photoadducts to specific DNA sequences. However, the functional consequences of these differing structures on psoralen photoreactivity are unknown. …”
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Prevalence and Molecular Characterization of Cysticercus tenuicollis Isolated from Some Intermediate Host in Kurdistan-Iraq by Abdullah Ahmed Hama, Rostam Hama Zorab, Fatima Mohammed Ali, Awaz M. Salih, Amer Abdullah Hassan

“…The selected cysts were preserved in 70% Ethanol for DNA extraction and molecular study,  The mt-CO1 gene was amplified with a conventional polymerase chain reaction (PCR), the PCR product purified and DNA sequencing for reverse and forward strands was determined by a genetic analyzer, the obtained sequences aligned with the DNA sequences of T. hydatigena in Iran, Turkey and Palestine, which deposited in GenBank under the following accession number (JQ710588), (JN827307) and ( KM032284) respectively. …”
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BAC-browser: the tool for synthetic biology by Tatiana A. Semashko, Gleb Y. Fisunov, Georgiy Y. Shevelev, Vadim M. Govorun

“…Its main tasks, such as the design of synthetic sequences and the assembly of DNA sequences from synthetic oligonucleotides, require specialized software. …”
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Specific PCR Primers Based on Cytochrome-b Gene for Molecular Detection of Javan Mongoose (Herpestes javanicus) by Sena Adi Subrata, Subeno Subeno, Atus Syahbudin

“…The design starts with selecting specific marker from 51 DNA sequences of cytochrome-b. The DNA sequences were of 19 Javan carnivore collected from GenBank (NCBI). …”
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Isolation of Lodderomyces elongisporus from the Catheter Tip of a Fungemia Patient in the Middle East by Suhail Ahmad, Zia U. Khan, Molly Johny, Najat M. Ashour, Wehad H. Al-Tourah, Leena Joseph, Rachel Chandy

“…The identity of the isolate was confirmed by phenotypic characteristics and ribosomal DNA sequencing.…”
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Molecular Identification of Fungal Communities in a Soil Cultivated with Vegetables and Soil Suppressiveness to Rhizoctonia solani by Silvana Pompéia Val-Moraes, Eliamar Aparecida Nascimbem Pedrinho, Eliana Gertrudes Macedo Lemos, Lucia Maria Carareto-Alves

“…This metagenomic DNA was used as a template to amplify fungal 18S rDNA sequences, and libraries were constructed in Escherichia coli by cloning PCR products. …”
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The Correlation between Gut Microbiota and Serum Metabolomic in Elderly Patients with Chronic Heart Failure by Zhenhua Wang, Zhaoling Cai, Markus W. Ferrari, Yilong Liu, Chengyi Li, Tianzhang Zhang, Guorong Lyu

“…The expression of inflammatory factors in blood was detected by ELISA. 16S rDNA sequencing was used to analyze the changes in microorganisms in the samples. …”
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Diversity across Seasons of Culturable Pseudomonas from a Desiccation Lagoon in Cuatro Cienegas, Mexico by Alejandra Rodríguez-Verdugo, Valeria Souza, Luis E. Eguiarte, Ana E. Escalante

“…A total of 70 Pseudomonas isolates across seasons were obtained, genotyped by fingerprinting (BOX-PCR), and taxonomically characterized by 16S rDNA sequencing. We found 35 unique genotypes, and two numerically dominant lineages (16S rDNA sequences) that made up 64% of the sample: P. cuatrocienegasensis and P. otitidis. …”
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