Immune and genomic biomarkers of immunotherapy response in cancer of unknown primary by Madhu Singh, Linda Mileshkin, Penelope Schofield, Bo Gao, Gary Richardson, Andrew Pattison, Rodney J Hicks, Niko Thio, Mark Warren, Colin Wood, Stephen B Fox, Anna DeFazio, Andrew Fellowes, Christopher Steer, David Bowtell, Nicholas Wilcken, Tharani Sivakumaran, Atara Posner, Dariush Etemadmoghadam, Krista Fisher, Samantha Webb, Christos S Karapetis, Ian M Collins, Narayan Karanth, Owen W J Prall, Richard William Tothill

“…Of GEP profiled cases, 33% (59/175) had a high IR gene-expression score. Of the DNA sequenced cases, 16% (32/203) had high TMB (>10 mutations/Mb), including two with mismatch repair deficiency. …”
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Traditional pediatric massage enhanced the skeletal muscle mass in OVA-exposed adolescent rats via regulating SCFAs-FFAR2-IGF-1/AKT pathway by Lin Lin, Siyuan Li, Que Liu, Xingxing Zhang, Ying Xiong, Shaoyun Zhao, Liyue Cao, Jiaxuan Gong, Yaping Liu, Rong Wu

“…GM and SCFAs production were detected by 16S rDNA sequencing and GC-MS, respectively. The protein and gene expressions of free fatty acid receptor 2 in SM were detected by using immunohistochemical staining and qRT-PCR, respectively. qRT-PCR was used to detect other relative gene expressions that were closely related with SM mass. …”
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Plasmodium falciparum transcription factor AP2-06B is mutated at high frequency in Southeast Asia but does not associate with drug resistance by Qiyang Shi, Changhong Wang, Wenluan Yang, Xiaoqin Ma, Jianxia Tang, Jiayao Zhang, Guoding Zhu, Yinlong Wang, Yaobao Liu, Xiaoqin He

“…One mutation, K3124R, was located in a PfAP2-06B AP2 domain.MethodsTo investigate transcriptional regulation by PfAP2-06B, ChIP-seq assays were performed on 3D7/PfAP2-06B-GFP schizonts using antibodies against GFP. The DNA sequences of the artemisinin-resistant CWX and the quinoline-resistant strains PfDd2 and Pf7G8 were analyzed for the genetic diversity of AP2-06B, compared with the Pf3D7 strain as a reference sequence. …”
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Characteristics and spatiotemporal changes in phenotypes and genotypes of extended-spectrum β-lactamases in Escherichia coli isolated from bloodstream infections in China from 2014... by Sayyed Salman, Hao Xu, Yunbo Chen, Jinru Ji, Zhiying Liu, Yonghong Xiao

“…The genetic type was determined using polymerase chain reaction (PCR) followed by DNA sequencing. Results Between 2014 and 2021, the prevalence of ESBL-positive E. coli steadily decreased from 61.2 to 49.6%. …”
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Basic Molecular Biology, Metabolic and Immunological Mechanisms of Fecal Microbiota Transplantation by O. V. Goloshchapov, A. V. Beynarovich, A. B. Chukhlovin, R. V. Klementeva, A. A. Shcherbakov, E. A. Bakin, I. K. Golubovskaya, N. D. Ventslovayte, O. S. Yudintseva, M. V. Gorchakova, V. E. Karev, M. A. Kucher, M. A. Suvorova, E. V. Svetkina, G. G. Rodionov, V. V. Gostev, S. V. Sidorenko, I. S. Moiseev, A. D. Kulagin

“…To monitor the basic effects of FMT, we performed targeted DNA sequencing of 16S rRNA gene (V3–V4) using MiSeq platform as well as multiplex real-time PCR, MS/gas chromatography technique, immunophenotyping of blood lymphocytes, histological and immunohistochemical techniques.Clinical case. …”
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Disproportionate Distribution of HBV Genotypes A and D and the Recombinant Genotype D/E in the High and Low HBV Endemic Regions of Uganda: A Wake-Up Call for Regional Specific HBV... by Hussein Mukasa Kafeero, Dorothy Ndagire, Ponsiano Ocama, Charles Drago Kato, Eddie Wampande, Henry Kajumbula, David Kateete, Abdul Walusansa, Ali Kudamba, Kigozi Edgar, Fred Ashaba Katabazi, Maria Magdalene Namaganda, Jamilu E. Ssenku, Hakim Sendagire

“…Purification of nested PCR products was performed using Purelink PCR product purification kit (Life Technologies, USA). Automated DNA sequencing was performed using BigDye Terminator v3.1 Cycle Sequencing Kit on 3130 Genetic Analyzer (Applied Biosystems, USA). …”
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Phase II trial of nab-paclitaxel plus ramucirumab in combination with nivolumab for unresectable advanced or recurrent gastric cancer after progression on first-line treatment incl... by Hidekazu Hirano, Naoki Takahashi, Yusuke Amanuma, Nobumi Suzuki, Daisuke Takahari, Takeshi Kawakami, Chie Kudo-Saito, Kengo Nagashima, Narikazu Boku, Ken Kato, Hirokazu Shoji

“…., flow cytometry, DNA sequencing [DNAseq]/RNA sequencing [RNAseq]). Recruitment started in November 2022. …”
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Relationship between mucin gene polymorphisms and different types of gallbladder stones by Gongqing Ren, Yongmao Fan, Ruizi Zhong, Gang Zou, Xiaojun Huang, Yue Zhang

“…We amplified SNP sites by polymerase chain reaction (PCR) using specific primer sets followed by DNA sequencing. Results The frequencies of MUC2 rs2856111 C/T genotype (OR = 3.81, 95%CI: 1.06–13.68) was higher than the control group. …”
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Therapeutic Potential of <i>Cajanus cajan</i> (L.) <i>Millsp</i>. Leaf Extract in Modulating Gut Microbiota and Immune Response for the Treatment of Inflammatory Bowel Disease by Mingzhang Lin, Linghua Piao, Zhendong Zhao, Li Liao, Dayong Wang, Haiwen Zhang, Xiande Liu

“…Subsequently, flow cytometry was employed to analyze the populations of various immune cells within the spleen, thereby assessing the impact of the CCLE on the systemic immune homeostasis of IBD mice. Finally, 16S rDNA sequencing was conducted to examine the composition and relative abundance of gut microbiota across different experimental groups. …”
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Characterization of CO1 and 18S rDNA genes from Indonesian native ornamental shrimp Caridina boehmei cultured in Jakarta by Ekky Ilham Romadhona, Novi Megawati, Handang Widantara, Sutanti Sutanti, Arif Rahman Ardiansyah, Kiki Mariya Dewi, Annisa Fitri Larassagita, Aditia Firman, Moh Risky, Rahmat Rahmat, Ayub Ayub

“…The CO1 sequencing produced fragments consisting of 25.84% adenine, 20.95% cytosine, 19.11% guanine, and 34.10% thymine. The 18S rDNA sequencing produced fragments consisting of 24.47% adenine, 26.05% cytosine, 28.16% guanine, and 21.32% thymine. …”
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Integrating 16S rDNA and metabolomics to uncover the therapeutic mechanism of electroacupuncture in type 2 diabetic rats by Zhang Yue, Wang Xiang, Deng Duping, Gong Yuanyuan, Chen Xuanyi, Li Juan, Li Juan, Hong Xiaojuan

“…Fecal microbial communities were analyzed by 16S rDNA sequencing, and short-chain fatty acids (SCFAs) were measured using gas chromatography–mass spectrometry (GC–MS). …”
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Decreasing of heterogeneity in black tiger prawn (Penaeus monodon) and white shrimp (Litopenaeus vannamei) broodstocks and their fry produced in hatchery by Bambang Widyo Prastowo, Rahayu Rahardianti, Evi Maftuti Nur

“…Amplification of black tiger shrimp mtDNA used primers designed based on 16S rDNA sequences, and the products were restricted by Nde II enzyme.  …”
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