Genome stability of the vaccine strain VAC∆6 by R. A. Maksyutov, S. N. Yakubitskiy, I. V. Kolosova, T. V. Tregubchak, A. N. Shvalov, E. V. Gavrilova, S. N. Shchelkunov

“…PCR and sequencing analysis of six DNA fragments corresponding to the regions of disrupted genes in VACΔ6 showed that all viral DNA sequences remained unchanged after 15 passages in 4647 cells.…”
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Development and progression association of various ophthalmic pathologies with the gut microbiome state (literature review) by G.N. Rezbaeva, A.E. Babushkin, O.I. Orenburkina, I.A. Gimranova

“…A huge study breakthrough of the gut microbiota species composition in a healthy person, as well as in persons with various pathologies, was obtained after a technique development for DNA sequencing. Modern researches focus on studying the effect of changes in the gut microbiome on human health, including his eyes. …”
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Integrating microbial profiling and machine learning for inference of drowning sites: a forensic investigation in the Northwest River by Qin Su, Xiaofeng Zhang, Xiaohui Chen, Zhonghao Yu, Weibin Wu, Qingqing Xiang, Chengliang Yang, Jian Zhao, Ling Chen, Quyi Xu, Chao Liu

“…We utilized 16S rDNA sequencing to analyze microbial diversity in water samples and lung tissues, revealing distinct microbial signatures associated with drowning sites. …”
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Dietary supplementation with Epimedium contributes to the improvement of hormone levels, gut microbiota, and serum metabolite composition in the Chinese forest musk deer (Moschus b... by Shan Xie, Shan Xie, Qinlin Yang, Zaixiang Ying, Zaixiang Ying, Mingcheng Cai, Wenqiao Fan, Hanyu Gao, Hanyu Gao, Xiaolan Feng, Yongjiang Wu

“…The results indicated that the inclusion of Epimedium in the diet increased dry matter intake (DMI) and improved the ratio of feed to gain (F/G), with an increase in fecal testosterone levels (p < 0.05). 16S rDNA sequencing analysis revealed that Epimedium enhanced the richness and diversity of the gut microbiota in the Chinese forest musk deer, increasing the relative abundance of beneficial bacteria such as Firmicutes, while reducing the relative abundance of the potentially pathogenic Proteobacteria (p < 0.05). …”
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Clinical and genetic drivers of oligo-metastatic disease in colon cancer by Alessandro Ottaiano, Mariachiara Santorsola, Roberto Sirica, Annabella Di Mauro, Antonella Di Carlo, Monica Ianniello, Francesco Sabbatino, Rosa Castiello, Francesca Del Peschio, Marco Cascella, Francesco Perri, Maurizio Capuozzo, Nicola Martucci, Edoardo Mercadante, Valentina Borzillo, Rossella Di Franco, Francesco Izzo, Vincenza Granata, Carmine Picone, Antonella Petrillo, Massimiliano Berretta, Salvatore Stilo, Luca Tarotto, Anna Chiara Carratù, Gerardo Ferrara, Madhura Tathode, Alessia Maria Cossu, Marco Bocchetti, Michele Caraglia, Guglielmo Nasti, Giovanni Savarese

“…OMD was defined as ≤3 lesions per organ, each with a total diameter <70 mm and none exceeding 25 mm. Tumor DNA sequencing by NGS utilized the TruSight Oncology 500 kit. …”
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The impact of an integrated intervention program combining drug therapy with water, sanitation, and hygiene (WASH) education on reinfection with intestinal parasitic infections amo... by Woottichai Nachaiwieng, Sangob Sanit, Nattharinee Kongta, Jassada Saingamsook, Suwit Duangmano, Sakorn Pornprasert, Pradya Somboon, Jintana Yanola

“…Detection of benzimidazole resistance linked to the beta-tubulin gene mutation in soil-transmitted helminths (STH) was performed using polymerase chain reaction (PCR) amplification and DNA sequencing. Mass drug administration (MDA) with albendazole was applied to both groups. …”
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Susceptibility to Ventricular Arrhythmias Resulting from Mutations in FKBP1B, PXDNL, and SCN9A Evaluated in hiPSC Cardiomyocytes by Hector Barajas-Martinez, Maya Smith, Dan Hu, Robert J. Goodrow, Colleen Puleo, Can Hasdemir, Charles Antzelevitch, Ryan Pfeiffer, Jacqueline A. Treat, Jonathan M. Cordeiro

“…Genetic analysis using next-generation DNA sequencing revealed heterozygous mutations in SCN9A, PXDNL, and FKBP1B in the proband. …”
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Fatty Acid Content and Profile in <i>Ulva lactuca</i> in Response to Exposure to Variable Growth Conditions in Indoor Photobioreactors by Nabeel Gnayem, Razan Unis, Rima Gnaim, Alexander Chemodanov, Álvaro Israel, Jallal Gnaim, Alexander Golberg

“…The taxonomic identity of <i>U. lactuca</i> was confirmed through DNA sequencing using 3 markers (rbcL, ITS, and tufa). …”
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Identification and characterization of Serratia nematophila and Acinetobacter guillouiae from putrid-skin disease lesions in farmed Chinese spiny frog (Quasipaa spinosa) by Ling Guo, Xiyu Jin, Dan Yang, Li Wei, Jie Chen, Zhihua Lin, Li Ma

“…Two pathogenic strains, GL-XJ7 and GL-XJ11, were isolated from disease lesions and identified as Serratia nematophila and Acinetobacter guillouiae, respectively, through morphological, physiological, biochemical, and molecular analyses including 16S rDNA sequencing and phylogenetic tree construction. Experimental infections revealed median lethal concentrations (LD50) over 72 h of 3.98×107 CFU/mL for S. nematophila strain GL-XJ7 and 3.16 × 106 CFU/mL for A. guillouiae strain GL-XJ11. …”
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EPIGENETICS TODAY AND TOMORROW by B. F. Vanyushin

“…DNA methylation in plants and animals carried out by site-specific enzymes cytosine DNA-methyltransferases produces 5-methylcytosine (m5C) residues in such DNA sequences as CG, СNG and СNN. Adenine DNA methylation also occurs in plants. …”
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Optimized methods for the targeted surveillance of extended-spectrum beta-lactamase-producing Escherichia coli in human stool by Sarah Gallichan, Sally Forrest, Esther Picton-Barlow, Claudia McKeown, Maria Moore, Eva Heinz, Nicholas A. Feasey, Joseph M. Lewis, Fabrice E. Graf

“…We found that enriching stool in a nutrient broth for 4 h, then plating the bacterial suspension on antibiotic-selective MacConkey agar, and finally extracting DNA from the bacteria using a specific DNA purification kit resulted in improved recovery of ESBL E. coli and high-quality DNA. Sequencing multiple isolates from stool allowed us to distinguish unambiguously and at high resolution between different variants of ESBL E. coli present in stool.…”
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New bitongling regulates gut microbiota to predict angiogenesis in rheumatoid arthritis via the gut-joint axis: a deep neural network approach by Yin Guan, Xiaoqian Zhao, Yun Lu, Yue Zhang, Yan Lu, Yue Wang

“…The study employed 16S ribosomal DNA (16S rDNA) sequencing to analyze gut microbiota composition, machine learning techniques to identify characteristic microbial taxa, and transcriptomic analysis (GSVA) to assess the impact on the VEGF signaling pathway. …”
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Assessment of upper respiratory and gut bacterial microbiomes during COVID-19 infection in adults: potential aerodigestive transmission by Hafez Al-Momani, Andrew Nelson, Hadeel Al Balawi, Dua’a Al Balawi, Iman Aolymat, Ashraf I. Khasawneh, Hala Tabl, Ayman Alsheikh, AbdelRahman M. Zueter, Jeffrey Pearson, Christopher Ward

“…ELISA Pepsin tests and Reflux Symptom Index scores (RSI) were conducted for EOR assessment. DNA sequencing of the V4 region of the 16 S rRNA gene was performed for microbiome analysis. …”
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Comprehensive genomic and transcriptomic profiling of pulmonary nodules in synchronous multiple primary lung cancer by Xingsheng Liu, Kun Qian, Lei Su, Xiaoru Tian, Xin Zhao, Tengteng Wang, Li Han, Zhenzhen Li, Peilong Zhang, Ruotian Wang, Baodong Liu, Yuanbo Li, Xiaogang Tan, Yi Zhang

“…Methods We performed targeted DNA sequencing on tumor specimens and conducted bulk RNA transcriptome analysis on 53 multiple nodules originating from 26 lung cancer patients. …”
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Joint fluid multi-omics improves diagnostic confidence during evaluation of children with presumed septic arthritis by Yolanda Peña-López, Naureen G. Tareen, Bo Zhang, Indu Raman, Carlos A. Arana, Chengsong Zhu, Yang Liu, Pratibha Selvakumar, Nicolai S. C. van Oers, Simrat Morris, Lora V. Hooper, Lawson A. B. Copley, Prithvi Raj

“…The combination of 16S DNA sequencing (p = 0.006), cytokine assays (p = 0.009) and autoantibody profiling (p = 0.02) were significantly distinct between those children with and without infections. …”
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Mutations in 23S rRNA gene associated with clarithromycin resistance in Helicobacter pylori clinical isolates from Saint-Petersburg by Starkova D.A., Gladyshev N.S., Polev D.E., Egorova S.A., Svarval A.V.

“…The PCR products (1402 bp) of the 23S rRNA gene were sequenced by Sanger approach. The DNA sequences were compared to the H. pylori 26695 reference using Unipro UGENE v.38.1. …”
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<i>Bacillus cabrialesii</i> subsp. <i>cabrialesii</i> Strain TE5: A Promising Biological Control Bacterium Against the Causal Agent of Spot Blotch in Wheat by Ixchel Campos-Avelar, Michelle Fabiela García Jaime, Pamela Helué Morales Sandoval, Fannie Isela Parra-Cota, Sergio de los Santos Villalobos

“…Genomic annotation using Rapid Annotation Using Subsystems Technology (RAST) and Rapid Prokaryotic Genome Annotation (Prokka) indicated the presence of 4615 coding DNA sequences (CDSs) distributed across 330 subsystems, which included gene families associated with biocontrol, stress response, and iron competition. …”
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Clinical and molecular analysis of JCPyV and BKPyV infection and associated risk of urothelial carcinoma development in the upper tract by Chun-Nun Chao, Chi-Feng Hung, Wei‑Hong Lai, Chun-Liang Tung, Wan-Yun Yeh, Kai-Wu Yang, Meilin Wang, Ya-Yan Lai, Pei-Lain Chen, Cheng-Huang Shen

“…Nested polymerase chain reaction and DNA sequencing were used to detect viral DNA and genotypes. …”
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Genome skimming provides evidence to accept two new genera (Apiaceae) separated from the Peucedanum s.l. by Bo-Ni Song, Chang-Kun Liu, Chang-Kun Liu, Jiao-Jiao Deng, Wei-Yan Tan, Song-Dong Zhou, Xing-Jin He

“…Therefore, we conducted a comprehensive taxonomic revision of taxa within the polyphyletic Peucedanum s.l. genus in this study.MethodsWe used two molecular datasets (103 plastomes and 43 nrDNA sequences) generated by genome skimming to reconstructed a reliable phylogenetic framework with high support and resolution. …”
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Exploring the Role of C-C Motif Chemokine Ligand-2 Single Nucleotide Polymorphism in Pulmonary Tuberculosis: A Genetic Association Study from North India by Sanjay K. Biswas, Mayank Mittal, Ekata Sinha, Vandana Singh, Nidhi Arela, Bharat Bajaj, Pramod K. Tiwari, Vishwa M. Katoch, Keshar K. Mohanty

“…The genotyping was carried out in 373 participants with pulmonary TB (PTB) and 248 healthy controls (HCs) for CCL2-2518 A>G and -362 G>C polymorphisms by PCR-RFLP and by melting curve analysis using fluorescence-labeled hybridization fluorescent resonance energy transfer (FRET) probes, respectively, followed by DNA sequencing in a few representative samples. Genotype and allele frequencies were compared by the chi-squared test and crude and Mantel-Haenszel (M-H) odds ratio (OR). …”
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