Evaluation of a Commercial Multiplex Real-Time PCR with Melting Curve Analysis for the Detection of <i>Mycobacterium tuberculosis</i> Complex and Five Nontuberculous Mycobacterial... by Keun Ju Kim, Yunhee Chang, Seung Gyu Yun, Myung-Hyun Nam, Yunjung Cho

“…These findings were confirmed via DNA sequencing. The assay had 100% sensitivity and specificity for <i>M. intracellulare</i>, <i>M. abscessus</i>, <i>M. massilense</i>, NTM, and MTBC, whereas it had 100% specificity and sensitivity of 90.9% and 75.0% for <i>M. avium</i> and <i>M. kansasii</i>, respectively. …”
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The co-occurrence patterns and assembly mechanisms of microeukaryotic communities in geothermal ecosystems of the Qinghai-Tibet Plateau by Bingjie Yan, Bingjie Yan, Xiaodong Li, Xiaodong Li, Nanqian Qiao, Nanqian Qiao, Zhen Da, Zhen Da, Jiajie Xu, Jiajie Xu, Chuanqi Jiang, Sang Ba, Sang Ba

“…In this study, we used high-throughput 18S rDNA sequencing in combination with environmental factor analysis to investigate the co-occurrence patterns, assembly mechanisms, and responses to environmental changes of microeukaryotic communities in sediment and water samples from 36 geothermal springs across different temperature gradients in southern Tibet. …”
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Comparative analysis of EGFR mutations in circulating tumor DNA and primary tumor tissues from lung cancer patients using BEAMing PCR by Duhita Mirikar, Nandini Banerjee, Kumar Prabhash, Rajiv Kumar Kaushal, Vanita Naronha, C. S. Pramesh, George Karimundackal, Amit Joshi, Swapnil Rane, Ranjan Basak

“…The advantage of BEAMing is its ability to identify mutated DNA sequences in cancer cells in the background of normal cell DNA contamination. …”
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Molecular identification of pfcytb, pfdhfr, and pvmrp1 mutations in imported malaria cases in Chile by Alan Oyarce, Lizbeth Huaccha, Esteban M. Cordero, Bárbara Parra, Jorge Fernández, Mario E. Soto-Marchant, Natalia T. Santis-Alay, María Isabel Jercic

“…The parasites target genes were amplified by polymerase chain reaction (PCR) out of total DNA extracted from patient blood samples and the amplicons submitted to DNA sequencing. All the genes analyzed had at least one mutation. …”
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Ophiostomatoid fungi associated with mites phoretic on bark beetles in Qinghai, China by Runlei Chang, Tuan A. Duong, Stephen J. Taerum, Michael J. Wingfield, XuDong Zhou, Z. Wilhelm de Beer

“…Mites were identified based on morphological characteristics, and fungi based on DNA sequences of four gene regions. In total, 173 mite individuals were collected belonging to 18 species in 11 genera. …”
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Gut microbiota and quantitative traits divergence at different altitude of long-tailed dwarf hamsters, Cricetulus longicaudatus by Yue Ren, Mengfan Tao, Xiaoliang Wang, Xinsheng Pu, Guangtong Guo, Kuiyou Chen, Bingyu Zhao, Yu Hou, Xin'gen Yang, Yumei Xu

“…We examined the diversity, abundance, and community structure of the gut microbiota of long-tailed dwarf hamsters from eight regions in Shanxi province during summer using 16S rDNA sequencing technology and analyzed the relationships between these microbiota and environmental variables as well as morphological indicators. …”
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Negative heterosis for meiotic recombination rate in spermatocytes of the domestic chicken Gallus gallus by L. P. Malinovskaya, K. V. Tishakova, T. I. Bikchurina, A. Yu. Slobodchikova, N. Yu. Torgunakov, A. A. Torgasheva, Y. A. Tsepilov, N. A. Volkova, P. M. Borodin

“…The decrease in recombination rate in F1 is probably determined by difficulties in homology matching between the DNA sequences of genetically divergent breeds. The suppression of recombination in the hybrids may impede gene flow between parapatric populations and therefore accelerate their genetic divergence.…”
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Vaginal Microbiota Diversity of Patients with Embryonic Miscarriage by Using 16S rDNA High-Throughput Sequencing by Linfen Xu, Lingna Huang, Chengying Lian, Huili Xue, Yanfang Lu, Xiujuan Chen, Yong Xia

“…To further dissect the microbiota of the vagina in the two groups, 16S rDNA sequencing was performed. Bioinformatics analysis showed that 1096 and 998 overlapped operational taxonomic units were identified from the embryonic miscarriage and control groups, respectively. …”
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Valorization of vermicompost with bacterial fermented chicken feather hydrolysate for the yield improvement of tomato plant: A novel organic combination by Ishita Biswas, Debasis Mitra, Ansuman Senapati, Debanjan Mitra, Sourav Chattaraj, Murshed Ali, Goutam Basak, Periyasamy Panneerselvam, Pradeep K. Das Mohapatra

“…Method Feather degrading bacteria were isolated and characterized using 16s-rDNA sequencing, and assessed for biochemical reactions, growth-promoting attributes and keratinase activity. …”
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CaGe: A Web-Based Cancer Gene Annotation System for Cancer Genomics by Young-Kyu Park, Tae-Wook Kang, Su-Jin Baek, Kwon-Il Kim, Seon-Young Kim, Doheon Lee, Yong Sung Kim

“…High-throughput genomic technologies (HGTs), including next-generation DNA sequencing (NGS), microarray, and serial analysis of gene expression (SAGE), have become effective experimental tools for cancer genomics to identify cancer-associated somatic genomic alterations and genes. …”
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Development and characterization of orthotopic patient-derived xenograft models of peritoneal metastatic mucinous appendiceal adenocarcinoma by I. Ito, V.K. Pattalachinti, A.M.G. Yousef, S. Chowdhury, M.M. Fanaeian, E. Haque, B.B. Gunes, M. Yousef, E.R. Salle, M.A. Zeineddine, S. Ji, R. Li, W. Wang, B.A. Helmink, M.W. Taggart, M.G. White, K.F. Fournier, N.W. Fowlkes, J.P. Shen

“…Comparison of histological, immunohistochemical, and molecular analyses including both RNA and DNA sequencing revealed that the PDX models recreate many of the features of metastatic AAs, but also displayed several differences between paired PDX and human tumors, highlighting the intratumoral heterogeneity of AAs within each patient. …”
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Allelic differences in the key genes of betalain biosynthesis in table beet accessions with contrasting root color from the VIR collection by A. S. Mikhailova, D. V. Sokolova, N. A. Shvachko, V. S. Popov, E. K. Khlestkina

“…Allelic differences were screened in the key genes of the betalain biosynthesis pathway among table beet accessions with various root color from the VIR collection using the Sanger DNA sequencing method.Results and discussion. For the first time we identified a nonsense mutation in the CYP76AD1 gene in cv. …”
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Gas chromatography-mass spectrometry in the taxonomy of Miscanthus by N. M. Slynko, N. V. Burmakina, O. M. Potseluyev, S. Yu. Kapustyanchik, G. Yu. Galitsin, T. N. Goryachkovskaya, L. V. Kuybida, S. V. Shekhovtsov, S. E. Peltek, V. K. Shumny

“…The results of chemotaxonomic analysis mostly matched those of DNA sequencing of a fragment of the plastid genome, which, moreover, allowed us to identify the species nature of the maternal plants used to obtain these hybrids. …”
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Study on Management of Blood Transfusion Therapy in Patients with Hereditary Spherocytosis by Shiyue Ma, Lingjian Tang, Chaoli Wu, Hui Tang, Xue Pu, Jinhong Niu

“…The fluorescence of EMA-labeled red blood cell (RBC) was decreased. DNA sequencing showed that His54Pro, Leu1858Val, and 6531-12C>T compound heterozygous mutations were present in the SPTA1 gene of patient I-1, Arg344Gln and c.609+86G>A heterozygous mutations were present in the SLC4A1 gene of patient II-1, and Leu2032Pro homozygous mutation was present in the SPTB gene of patient III-1. …”
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Toward an Efficient Differentiation of Two <i>Diaporthe</i> Strains Through Mass Spectrometry for Fungal Biotyping by Kathleen Hernández-Torres, Daniel Torres-Mendoza, Gesabel Navarro-Velasco, Luis Cubilla-Rios

“…Traditionally, the identification of fungi at the species level has been carried out with molecular tools such as DNA sequencing, but it still represents a huge challenge today due to the heterogeneity of the fungal kingdom, making the task of identification a complex and difficult process. …”
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New insights into the stipitate hydnoid fungi Sarcodon, Hydnellum, and the formerly informally defined Neosarcodon, with emphasis on the edible species marketed in Southwest China by Di Wang, Hui Feng, Jie Zhou, Tian-Hai Liu, Zhi-Yuan Zhang, Ying-Yin Xu, Jie Tang, Wei-Hong Peng, Xiao-Lan He

“…In the present study, specimens collected in the field and purchased from the markets in Southwest China were analyzed based on morphological characters and DNA sequences. Phylogeny of the traditional Sarcodon s. lat. and Hydnellum s. lat. was reconstructed from the combined internal transcribed spacer (ITS), nuclear large ribosomal subunit (nLSU) and RNA polymerase II second largest subunit (RPB2) dataset based on expanded samples to reevaluate the taxonomic placements of the two genera. …”
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Characterizations of cytokines and viral genomes in serum of patients with Dabie bandavirus infection by Zefeng Dong, Man Yuan, Yueping Xing, Hongkai Zhang, Qiang Shen

“…The genomes of Dabie bandavirus were amplified using a designated primer pool. The DNA sequencing libraries were prepared using a ligation method. …”
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Identification of <i>Tinomiscium petiolare</i> from Vietnam using the DNA barcode by B. B. Thinh, R. V. Doudkin, L. D. Chac, H. V. Chinh, Q. V. Hoi, N. T. Lien

“…In recent years, DNA barcoding has been known to be a fast and sensitive method for identifying species at any stage of development, using short DNA sequences. In this study we have performed the identification of T. petiolare specimens in Vietnam based on the sequence analysis of 4 DNA barcode loci: ITS, matK, rbcL and rpoC.Materials and methods. …”
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Analysis of Bacterial Diversity in Different Heavy Oil Wells of a Reservoir in South Oman with Alkaline pH by Biji Shibulal, Saif N. Al-Bahry, Yahya M. Al-Wahaibi, Abdulkadir E. Elshafie, Ali S. Al-Bemani, Sanket J. Joshi

“…It can be concluded that the microbial community was different in all the 10 soil samples with Bacillus and Paenibacillus sp. as predominating genus. The 16S rDNA sequencing of cultivable spore-forming bacteria identified the hydrocarbon utilizing bacteria as Bacillus and Paenibacillus sp. and the nucleotide sequences were submitted to NCBI GenBank under accession numbers KP119097–KP119115. …”
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Transitioning from wet lab to artificial intelligence: a systematic review of AI predictors in CRISPR by Ahtisham Fazeel Abbasi, Muhammad Nabeel Asim, Andreas Dengel

“…Abstract The revolutionary CRISPR-Cas9 system leverages a programmable guide RNA (gRNA) and Cas9 proteins to precisely cleave problematic regions within DNA sequences. This groundbreaking technology holds immense potential for the development of targeted therapies for a wide range of diseases, including cancers, genetic disorders, and hereditary diseases. …”
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