Exploring the Pharmacokinetics and Gut Microbiota Modulation of Hesperidin and Nobiletin from Mandarin Orange Peel in Experimental Dogs: A Pilot Study by Jun Nakahigashi, Makoto Kurikami, Satomi Iwai, Sadahiko Iwamoto, Shou Kobayashi, Eiji Kobayashi

“…Analysis included microbiota profiling via 16S rDNA sequencing and observational assessment of cognitive indicators in a pilot study involving senior dogs with Alzheimer’s disease. …”
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Impact of meropenem exposure on fluoroquinolone and carbapenem resistance in Pseudomonas aeruginosa infection in inpatients in a Japanese university hospital: Insights into oprD mu... by Tadanori Yamochi, Kazuhisa Ugajin, Rintaro On, Sho Inoue, Hiromi Ikeda, Toshiko Yamochi, Masafumi Takimoto, Issei Tokimatsu

“…Quantitative PCR was performed to analyze oprD, mexB, mexC, mexE, and mexY expression. DNA sequencing was performed to identify mutations. Results: Eight of nine strains were metallo-β-lactamase (MBL) negative, and one strain was MBL positive. …”
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Enterotype Bacteroides Is Associated with a High Risk in Patients with Diabetes: A Pilot Study by Jiajia Wang, Wenjuan Li, Chuan Wang, Lingshu Wang, Tianyi He, Huiqing Hu, Jia Song, Chen Cui, Jingting Qiao, Li Qing, Lili Li, Nan Zang, Kewei Wang, Chuanlong Wu, Lin Qi, Aixia Ma, Huizhen Zheng, Xinguo Hou, Fuqiang Liu, Li Chen

“…Fecal samples of each subject were also collected and were processed for 16S rDNA sequencing. Multiple logistic regression analysis was used to determine the associations of enterotypes with type 2 diabetes. …”
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Antimicrobial Susceptibility Testing of Metronidazole and Clindamycin against Gardnerella vaginalis in Planktonic and Biofilm Formation by Ting Li, Zhan Zhang, Fengjuan Wang, Yuanhui He, Xiaonan Zong, Huihui Bai, Zhaohui Liu

“…Sequence data of clinical G. vaginalis isolates were confirmed by comparing 16S rDNA sequences. Subsequently, clinical isolates were evaluated for antimicrobial susceptibilities in vitro to metronidazole and clindamycin at planktonic and biofilm levels. …”
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A meta-analysis of MHC diversity in Pan troglodytes verus by Christelle Vangenot, Alicia Sanchez‑Mazas

“…We performed a meta-analysis of the Major Histocompatibility Complex (MHC) diversity for locus A, B, C, DQB1, DPB1 and DRB1 in Chimpanzees by selecting cohorts of animals for which MHC genotypes were available in publications and by extracting DNA sequences available on the IPD-MHC database. The MHC genes play a key role in the immune responses and are located on the short arm of chromosome 6. …”
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Mutational Analysis of KCNQ1 Gene in Type 2 Diabetes Mellitus: A Case-control Study by Ankush Ganjewar, Ishwar B Bagoji, Sharan Badiger, Rudragouda B Bulagouda, Gurushnatappa Kadakol

“…Amplification of DNA products were analysed using Sanger-based DNA sequencing. Results: Sequencing analysis revealed g.6956 C>T and c.C1956T mutations in seven patients. …”
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Collection of microorganisms of ICG SB RAS as a genetic resource for biotechnology by A. V. Bryanskaya, Y. E. Uvarova, A. S. Rozanov, N. M. Slynko, V. N. Shlyakhtun, K. V. Starostin, E. A. Demidov, E. V. Lazareva, O. P. Taran, S. E. Peltek

“…The creation of personal mass spectra of protein profiles of the studied strains of the collection was carried out. The resulting DNA sequences of the strains are deposited in the GenBank. …”
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Enabling regulatory policy globally will promote realization of the potential of animal biotechnology by Eric Hallerman, Justin Bredlau, Luiz Sergio A. Camargo, Maria Lucia Zaidan Dagli, Margaret Karembu, Daniel Kovich, Anne Ndanu Muia, Mariana L. Murrone, Pedro Jesus Rocha-Salavarrieta, Rhodora Romero-Aldemita, Mark Tizard, Mark Walton, Diane Wray-Cahen

“…The advent of genome editing techniques has revolutionized the scientific approach to introducing changes into DNA sequences and how biotechnology can be used to enhance agricultural breeding. …”
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Genome stability of the vaccine strain VAC∆6 by R. A. Maksyutov, S. N. Yakubitskiy, I. V. Kolosova, T. V. Tregubchak, A. N. Shvalov, E. V. Gavrilova, S. N. Shchelkunov

“…PCR and sequencing analysis of six DNA fragments corresponding to the regions of disrupted genes in VACΔ6 showed that all viral DNA sequences remained unchanged after 15 passages in 4647 cells.…”
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Telomere Length Is Associated With Adverse Atrial Remodeling in Patients With Atrial Fibrillation by Kabita Pradhan, Balram Neupane, Paul Niehues, Martin Kirschner, Fabian Beier, Chao‐Chung Kuo, Erika Anneliese Hilbold, Christian Bär, Oana‐Maria Thoma, Maximilian Waldner, Margherita Vieri, Tim H. Brümmendorf, Vithurithra Tharmapalan, Wolfgang Wagner, Michael Kleines, Mahdi Emrani, Matthias Daniel Zink, Andreas Napp, Nikolaus Marx, Michael Gramlich

“…Telomeres, which are short, repeated DNA sequences at the end of chromosomes, are known to act as a biological aging marker. …”
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Clinical utility and predictive value of cerebrospinal fluid cell-free DNA profiling in non-small cell lung cancer patients with leptomeningeal metastasis by Sheng-Kai Liang, Wei-Yu Liao, Jin-Yuan Shih, Chia-Lin Hsu, Ching-Yao Yang, Shang-Gin Wu, Yen-Ting Lin, Yueh-Feng Wen, Lun-Che Chen, Yen-Fu Chen, Ya-Fang Chen, Yen-Heng Lin, Chong-Jen Yu

“…Among 25 enrolled patients, 22 (88.0 %) had EGFR mutations, while three (12.0 %) had EML4-ALK fusion, KIF5B-RET fusion, and ERBB2 A775_G776insSVMA. CSF cfDNA sequencing of 27 samples (from 25 patients) all confirmed their original driver mutations. …”
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Assessing microbial biodiversity in oil palm seedlings using oil palm waste compost mixed media by Siti Suliza Salamat, Mohd Zulkhairi Mohd Yusoff, Mohd Ali Hassan Hassan, Mohd Huzairi Mohd Zainuddin, Yoshihito Shirai, Ahmad Husni Mohd Hanif, Mohd Shahkhirat Norizan, Fatini Mat Arisah, Mohammed Abdillah Ahmad Farid, Toshinari Maeda, Mohd Noor Mat Isa, Mohd Faizal Abu Bakar

“…Method: The study compared microbial biodiversity in soil media with compost amendments by employing DNA sequencing to assess microbial communities. The analysis focused on bacterial species richness and the prevalence of key phyla, including Proteobacteria, Actinobacteria, Acidobacteria, and others. …”
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Oral-Delivery Lactococcus lactis expressing cherry fusion lactoferrin peptides against infection of avian pathogenic Escherichia coli in chickens by Zhuoran Li, Xueying Wang, Dianzhong Zheng, Fuzhen Han, Yue Li, Han Zhou, Jiaxuan Li, Wen Cui, Yanping Jiang, Xiaona Wang, Weichun Xie, Lijie Tang

“…Additionally, 16S rDNA sequencing data revealed that LL-EFLmC was able to restore the intestinal flora that had been disrupted by APEC-O78. …”
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Dietary supplementation with Epimedium contributes to the improvement of hormone levels, gut microbiota, and serum metabolite composition in the Chinese forest musk deer (Moschus b... by Shan Xie, Shan Xie, Qinlin Yang, Zaixiang Ying, Zaixiang Ying, Mingcheng Cai, Wenqiao Fan, Hanyu Gao, Hanyu Gao, Xiaolan Feng, Yongjiang Wu

“…The results indicated that the inclusion of Epimedium in the diet increased dry matter intake (DMI) and improved the ratio of feed to gain (F/G), with an increase in fecal testosterone levels (p < 0.05). 16S rDNA sequencing analysis revealed that Epimedium enhanced the richness and diversity of the gut microbiota in the Chinese forest musk deer, increasing the relative abundance of beneficial bacteria such as Firmicutes, while reducing the relative abundance of the potentially pathogenic Proteobacteria (p < 0.05). …”
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Clinical and genetic drivers of oligo-metastatic disease in colon cancer by Alessandro Ottaiano, Mariachiara Santorsola, Roberto Sirica, Annabella Di Mauro, Antonella Di Carlo, Monica Ianniello, Francesco Sabbatino, Rosa Castiello, Francesca Del Peschio, Marco Cascella, Francesco Perri, Maurizio Capuozzo, Nicola Martucci, Edoardo Mercadante, Valentina Borzillo, Rossella Di Franco, Francesco Izzo, Vincenza Granata, Carmine Picone, Antonella Petrillo, Massimiliano Berretta, Salvatore Stilo, Luca Tarotto, Anna Chiara Carratù, Gerardo Ferrara, Madhura Tathode, Alessia Maria Cossu, Marco Bocchetti, Michele Caraglia, Guglielmo Nasti, Giovanni Savarese

“…OMD was defined as ≤3 lesions per organ, each with a total diameter <70 mm and none exceeding 25 mm. Tumor DNA sequencing by NGS utilized the TruSight Oncology 500 kit. …”
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Susceptibility to Ventricular Arrhythmias Resulting from Mutations in FKBP1B, PXDNL, and SCN9A Evaluated in hiPSC Cardiomyocytes by Hector Barajas-Martinez, Maya Smith, Dan Hu, Robert J. Goodrow, Colleen Puleo, Can Hasdemir, Charles Antzelevitch, Ryan Pfeiffer, Jacqueline A. Treat, Jonathan M. Cordeiro

“…Genetic analysis using next-generation DNA sequencing revealed heterozygous mutations in SCN9A, PXDNL, and FKBP1B in the proband. …”
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Fatty Acid Content and Profile in <i>Ulva lactuca</i> in Response to Exposure to Variable Growth Conditions in Indoor Photobioreactors by Nabeel Gnayem, Razan Unis, Rima Gnaim, Alexander Chemodanov, Álvaro Israel, Jallal Gnaim, Alexander Golberg

“…The taxonomic identity of <i>U. lactuca</i> was confirmed through DNA sequencing using 3 markers (rbcL, ITS, and tufa). …”
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Identification and characterization of Serratia nematophila and Acinetobacter guillouiae from putrid-skin disease lesions in farmed Chinese spiny frog (Quasipaa spinosa) by Ling Guo, Xiyu Jin, Dan Yang, Li Wei, Jie Chen, Zhihua Lin, Li Ma

“…Two pathogenic strains, GL-XJ7 and GL-XJ11, were isolated from disease lesions and identified as Serratia nematophila and Acinetobacter guillouiae, respectively, through morphological, physiological, biochemical, and molecular analyses including 16S rDNA sequencing and phylogenetic tree construction. Experimental infections revealed median lethal concentrations (LD50) over 72 h of 3.98×107 CFU/mL for S. nematophila strain GL-XJ7 and 3.16 × 106 CFU/mL for A. guillouiae strain GL-XJ11. …”
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EPIGENETICS TODAY AND TOMORROW by B. F. Vanyushin

“…DNA methylation in plants and animals carried out by site-specific enzymes cytosine DNA-methyltransferases produces 5-methylcytosine (m5C) residues in such DNA sequences as CG, СNG and СNN. Adenine DNA methylation also occurs in plants. …”
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New bitongling regulates gut microbiota to predict angiogenesis in rheumatoid arthritis via the gut-joint axis: a deep neural network approach by Yin Guan, Xiaoqian Zhao, Yun Lu, Yue Zhang, Yan Lu, Yue Wang

“…The study employed 16S ribosomal DNA (16S rDNA) sequencing to analyze gut microbiota composition, machine learning techniques to identify characteristic microbial taxa, and transcriptomic analysis (GSVA) to assess the impact on the VEGF signaling pathway. …”
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