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341
Urbanization enhances consumer protist-driven ARGs dissemination in riverine ecosystems
Published 2025-01-01“…We utilized metagenomic and amplicon (16S and 18S rDNA) sequencing to investigate the relationships among microeukaryotic algae, consumer protists, bacterial communities, and the resistome. …”
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342
Telomere length varies with sex, hatching rank and year of birth in the Little Owl, Athene noctua
Published 2023-11-01“…Telomeres are non-coding DNA sequences located at the end of linear chromosomes, protecting genome integrity. …”
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343
Taxonomic assessment of the Oxytropis species from South-East of Kazakhstan
Published 2018-04-01“…Plant materials of O. almaatensis were collected from 2 populations in two neighboring Gorges in Trans Ili Alatau Mountains, O. glabra plant material was obtained from Herbarium of the Department of Biodiversity and Bioresources, al-Farabi Kazakh National University. Based on DNA sequences of ITS the phylogenetic and network relationships were investigated by using Neighbor Joining and Median Joining methods, respectively. …”
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344
Analysis of TGFBI Gene Mutations in Three Chinese Families with Corneal Dystrophy
Published 2019-01-01“…All 17 exons of the TGFBI gene, and the exon-intron junctions were examined by polymerase chain reaction (PCR) and direct DNA sequencing to identify and analyse gene mutations. …”
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345
Identification of Houge type of X-linked syndromic mental retardation caused by CNKSR2 truncated variants
Published 2025-02-01“…To verify the impact of these variants, we used real-time PCR and minigenes consisting of exon 14, intron 14, and exon 15 from both the wild-type and the c.1658-3_1676del DNA sequences. Results In this study, we reported two Chinese boys with MRXSHG and described some rare MRXSHG phenotypes, such as delayed bone age, slightly widened right fissure, and an underdeveloped right temporal lobe, characterized by reduced growth and volume compared to typical development. …”
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346
Temporal Study of Environmental DNA and Acoustic Data Reveals Coexistence of Sympatric Bat Species in a North American Ecosystem
Published 2024-11-01“…We used two different methods to assess niche partitioning among insectivorous bats at a site in the San Francisco Bay Area, California: (1) eDNA sequencing of bat feces that were collected weekly from a bat roost, and (2) nightly acoustic recordings of ultrasonic bat calls from recorders at multiple sites. …”
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347
Traces of archaic mitochondrial lineages persist in Austronesian-speaking Formosan populations.
Published 2005-08-01“…Interestingly, from complete mtDNA sequencing information, most B4a lineages were associated with three coding region substitutions, defining a new subclade, B4a1a, that endorses the origin of Polynesian migration from Taiwan. …”
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348
Liberties of the genome: insertions of mitochondrial DNA fragments into nuclear genome
Published 2024-09-01“…The constant flow of functionally inactive DNA sequences from mitochondria into the nucleus and its significance could be studied in view of the modern concepts of evolutionary theory suggesting non-adaptive complexity and the key role of stochastic processes in the formation of genomic structure.…”
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349
Cloning and expression analysis of the glucocorticoid receptors and the effects of cortisol in sex differentiation in protogynous hermaphroditic swamp eel, Monopterus albus
Published 2025-01-01“…We cloned and characterized the full-length cDNA sequences of glucocorticoid receptor genes, gr1 and gr2. …”
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350
Mutant Prevention Concentration of Ciprofloxacin against Klebsiella pneumoniae Clinical Isolates: An Ideal Prognosticator in Treating Multidrug-Resistant Strains
Published 2019-01-01“…The detection of qnr genes for 24 isolates and DNA sequencing for six isolates were performed. Results. …”
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351
The biodegradation of low-density polyethylene by Bacillus species
Published 2025-02-01“…Four Bacillus species were isolated from different dumpsites in Owerri Metropolis, and identified using 16S rDNA sequencing, as B.pumilus, B.siamensis, B.coagulans and B. subtilis. …”
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352
Distribution of the arctic variant of the CPT1A gene in indigenous populations of Siberia
Published 2016-12-01“…Five polymorphic loci relevant to the haplotypic structure of CPT1A gene (rs2278908, rs2278907, rs2924699, rs7112615 and rs2229738) were revealed by high-throughput DNA sequencing in addition to locus rs80356779 studied here. …”
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353
Influence of breed and environment on leukocyte telomere length in cattle
Published 2024-04-01“…Telomeres are elements found at the ends of chromosomes, consisting of repetitive DNA sequences, several thousand base pairs in length, coupled with nucleoprotein complexes. …”
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354
Recent updates of centromere proteins in hepatocellular carcinoma: a review
Published 2025-02-01“…Centromeres are considered special deoxyribonucleic acid (DNA) sequences with highly repetitive sequences that are physically connected to the spindle during cell division, ensuring equal division of genetic material between daughter cells. …”
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355
Characterization of Integrons and Antimicrobial Resistance in Escherichia coli Sequence Type 131 Isolates
Published 2020-01-01“…The presence and characterization of class 1, 2, and 3 integrons, as well as promotor of gene cassettes and other antimicrobial resistance genes, were detected by PCR and DNA sequencing. Transfer of integrons was carried out using a broth culture mating method. …”
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356
Characterization of Pathogenic Bacteria Isolated from Sudanese Banknotes and Determination of Their Resistance Profile
Published 2018-01-01“…Genotypic identification was carried out using PCR and 16S rDNA sequencing. Antibiotic resistance genes of some isolates were detected using PCR technique. …”
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357
The Sequence of Prostaglandin-Endoperoxide Synthase 2 Gene in Women with Cervicitis
Published 2022-11-01“…The conventional PCR was used for gene detection and DNA sequencing. The mean± SD of Ca125, CA15.3 and CRP showed a highly significant differences between patient (30.33±1.09), (22.82±1.07), (88.06±5.88) respectively and the healthy control (19.40±0.68), (18.34±0.77), (6.73±0.12) respectively-value >0.001. …”
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358
SequenceCraft: machine learning-based resource for exploratory analysis of RNA-cleaving deoxyribozymes
Published 2025-01-01“…Abstract Background Deoxyribozymes or DNAzymes represent artificial short DNA sequences bearing many catalytic properties. In particular, DNAzymes able to cleave RNA sequences have a huge potential in gene therapy and sequence-specific analytic detection of disease markers. …”
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359
Identification and genetic analysis of the recent Avian pox viruses from infected pigeons and chickens
Published 2025-01-01“…Results The polymerase chain reaction (PCR) assay was able to detect APV in all the tested samples; 12 positive samples (6 chicken flocks and 6 pigeon flocks) were selected for DNA sequencing. The sequences were submitted to GenBank with accession numbers OR027032 to OR027043. …”
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360
Cell-free fat extract improves embryo development and clinical outcomes in older women with previous in-vitro fertilization failure
Published 2025-01-01“…In addition, we performed immunofluorescence staining, multiplex immunoassay, whole-genome amplification and DNA sequencing, time-lapse embryo monitoring, and in vitro experiments. …”
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