Coupling of an Au@AgPt nanozyme array with an micrococcal nuclease-specific responsiveness strategy for colorimetric/SERS sensing of Staphylococcus aureus in patients with sepsis by Xueqin Huang, Yingqi Yang, Hanlin Zhou, Liping Hu, Annan Yang, Hua Jin, Biying Zheng, Jiang Pi, Jun Xu, Pinghua Sun, Huai-Hong Cai, Xujing Liang, Bin Pan, Junxia Zheng, Haibo Zhou

“…In the presence of S. aureus, the secreted MNase preferentially cut the nucleobase AT-rich regions of DNA sequences on magnetic beads (MBs) to release alkaline phosphatase (ALP), which subsequently mediated the oxTMB reduction for inducing the colorimetric/SERS signal fade away. …”
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Interactions of miR-34b/c and TP53 Polymorphisms on the Risk of Intracranial Aneurysm by Lijuan Li, Xiutian Sima, Peng Bai, Lushun Zhang, Hong Sun, Weibo Liang, Jianxing Liu, Lin Zhang, Linbo Gao

“…., miR-34b/c rs4938723 and TP53 Arg72-Pro) were genotyped by polymerase chain reaction-restriction fragment length polymorphism assay and DNA sequencing. We found that the CC genotype of miR-34b/c rs4938723 was significantly associated with a decreased risk of IA compared with the TT genotype. …”
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Catfishes from the North-Western Part of Lake Tanganyika: Contribution to a Reference Library of DNA Barcodes by Archimède Mushagalusa Mulega, Arthur F. Boom, Imane Rahmouni, Hocein Bazairi, Abdelaziz Benhoussa, Maarten P. M. Vanhove, Maarten Van Steenberge

“…We identified 11 species using morphology, compared DNA sequences with those available on GenBank, and employed Automatic Barcode Gap Discovery (ABGD) and phylogenetic approaches. …”
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Diversity of bacterial symbionts associated with the tropical plant bug Monalonion velezangeli (Hemiptera: Miridae) revealed by high-throughput 16S-rRNA sequencing by Navarro-Escalante, Lucio, Benavides, Pablo, Acevedo, Flor E.

“…Based on PCR screening and DNA sequencing of the wsp gene, Wolbachia infection was confirmed in almost 80% of samples, and represented by two different isolates or strains within the supergroup B. …”
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Urbanization enhances consumer protist-driven ARGs dissemination in riverine ecosystems by Bob Adyari, Lanping Zhang, Ana Maravić, Jiaxin Chen, Laiyi Li, Mahmoud Gad, Chang-Ping Yu, Anyi Hu

“…We utilized metagenomic and amplicon (16S and 18S rDNA) sequencing to investigate the relationships among microeukaryotic algae, consumer protists, bacterial communities, and the resistome. …”
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Telomere length varies with sex, hatching rank and year of birth in the Little Owl, Athene noctua by Criscuolo, François, Fache, Inès, Scaar, Bertrand, Zahn, Sandrine, Bleu, Josefa

“…Telomeres are non-coding DNA sequences located at the end of linear chromosomes, protecting genome integrity. …”
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Taxonomic assessment of the Oxytropis species from South-East of Kazakhstan by S. Almerekova, S. Abugalieva, N. Mukhitdinov

“…Plant materials of O. almaatensis were collected from 2 populations in two neighboring Gorges in Trans Ili Alatau Mountains, O. glabra plant material was obtained from Herbarium of the Department of Biodiversity and Bioresources, al-Farabi Kazakh National University. Based on DNA sequences of ITS the phylogenetic and network relationships were investigated by using Neighbor Joining and Median Joining methods, respectively. …”
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Analysis of TGFBI Gene Mutations in Three Chinese Families with Corneal Dystrophy by Feng Zhao, Yuan Liu, Tao Guan

“…All 17 exons of the TGFBI gene, and the exon-intron junctions were examined by polymerase chain reaction (PCR) and direct DNA sequencing to identify and analyse gene mutations. …”
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Identification of Houge type of X-linked syndromic mental retardation caused by CNKSR2 truncated variants by Si-Hua Chang, Jie-Yuan Jin, Yi-Qiao Hu, Run-Yan Wang, Rong Xiang, Xia Wang

“…To verify the impact of these variants, we used real-time PCR and minigenes consisting of exon 14, intron 14, and exon 15 from both the wild-type and the c.1658-3_1676del DNA sequences. Results In this study, we reported two Chinese boys with MRXSHG and described some rare MRXSHG phenotypes, such as delayed bone age, slightly widened right fissure, and an underdeveloped right temporal lobe, characterized by reduced growth and volume compared to typical development. …”
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Temporal Study of Environmental DNA and Acoustic Data Reveals Coexistence of Sympatric Bat Species in a North American Ecosystem by Vrinda M. Suresh, Trevor Hébert, Kirsten Isabel Verster, Elizabeth A. Hadly

“…We used two different methods to assess niche partitioning among insectivorous bats at a site in the San Francisco Bay Area, California: (1) eDNA sequencing of bat feces that were collected weekly from a bat roost, and (2) nightly acoustic recordings of ultrasonic bat calls from recorders at multiple sites. …”
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Liberties of the genome: insertions of mitochondrial DNA fragments into nuclear genome by M. V. Golubenko, V. P. Puzyrev

“…The constant flow of functionally inactive DNA sequences from mitochondria into the nucleus and its significance could be studied in view of the modern concepts of evolutionary theory suggesting non-adaptive complexity and the key role of stochastic processes in the formation of genomic structure.…”
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Mutant Prevention Concentration of Ciprofloxacin against Klebsiella pneumoniae Clinical Isolates: An Ideal Prognosticator in Treating Multidrug-Resistant Strains by B. Aditi Priyadarshini, Krishnan Mahalakshmi, Venkatesan Naveen Kumar

“…The detection of qnr genes for 24 isolates and DNA sequencing for six isolates were performed. Results. …”
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The biodegradation of low-density polyethylene by Bacillus species by Felicia E. Uwakwe, Tobias I.N. Ezejiofor, Ernest A.A. Anyalogbu, Toochukwu E. Ogbulie

“…Four Bacillus species were isolated from different dumpsites in Owerri Metropolis, and identified using 16S rDNA sequencing, as B.pumilus, B.siamensis, B.coagulans and B. subtilis. …”
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Distribution of the arctic variant of the CPT1A gene in indigenous populations of Siberia by B. A. Malyarchuk, M. V. Derenko, G. A. Denisova, A. N. Litvinov

“…Five polymorphic loci relevant to the haplotypic structure of CPT1A gene (rs2278908, rs2278907, rs2924699, rs7112615 and rs2229738) were revealed by high-throughput DNA sequencing in addition to locus rs80356779 studied here. …”
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Influence of breed and environment on leukocyte telomere length in cattle by N. S. Yudin, A. V. Igoshin, G. A. Romashov, A. A. Martynov, D. M. Larkin

“…Telomeres are elements found at the ends of chromosomes, consisting of repetitive DNA sequences, several thousand base pairs in length, coupled with nucleoprotein complexes. …”
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Recent updates of centromere proteins in hepatocellular carcinoma: a review by Zhongyuan Yang, Wenjiao Chen, Yunhui Liu, Yuxin Niu

“…Centromeres are considered special deoxyribonucleic acid (DNA) sequences with highly repetitive sequences that are physically connected to the spindle during cell division, ensuring equal division of genetic material between daughter cells. …”
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Characterization of Integrons and Antimicrobial Resistance in Escherichia coli Sequence Type 131 Isolates by Jiangqing Huang, Fangjun Lan, Yanfang Lu, Bin Li

“…The presence and characterization of class 1, 2, and 3 integrons, as well as promotor of gene cassettes and other antimicrobial resistance genes, were detected by PCR and DNA sequencing. Transfer of integrons was carried out using a broth culture mating method. …”
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Characterization of Pathogenic Bacteria Isolated from Sudanese Banknotes and Determination of Their Resistance Profile by Noha Ahmed Abd Alfadil, Malik Suliman Mohamed, Manal M. Ali, El Amin Ibrahim El Nima

“…Genotypic identification was carried out using PCR and 16S rDNA sequencing. Antibiotic resistance genes of some isolates were detected using PCR technique. …”
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The Sequence of Prostaglandin-Endoperoxide Synthase 2 Gene in Women with Cervicitis by Maha A. Ahlial, Nazar Sh. Mohammed, Maysara S. Khalaf

“…The conventional PCR was used for gene detection and DNA sequencing. The mean± SD of Ca125, CA15.3 and CRP showed a highly significant differences between patient (30.33±1.09), (22.82±1.07), (88.06±5.88) respectively and the healthy control (19.40±0.68), (18.34±0.77), (6.73±0.12) respectively-value >0.001. …”
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Identification and genetic analysis of the recent Avian pox viruses from infected pigeons and chickens by Amira M. Helal, Osama Mahana, Ali Zanaty, Karim Selim, Mohamed. E. Taha, Mostafa M. Saleh, Mohamed I. Hassan, Mona S. Abdallah

“…Results The polymerase chain reaction (PCR) assay was able to detect APV in all the tested samples; 12 positive samples (6 chicken flocks and 6 pigeon flocks) were selected for DNA sequencing. The sequences were submitted to GenBank with accession numbers OR027032 to OR027043. …”
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