Vitamin D Receptor Gene Polymorphisms Influence T1D Susceptibility among Pakistanis by Maryam Mukhtar, Andleeb Batool, Abdul Wajid, Iram Qayyum

“…Genotypes were identified by DNA sequencing and PCR-RFLP. Results. The allelic and genotypic frequencies of FokΙ and ApaI were significantly associated with T1D (p<0.001) development. …”
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DNA alterations in ovarian adult granulosa cell tumours: A scoping review protocol. by Sven Karstensen, Karsten Kaiser, Caroline Moos, Tim Svenstrup Poulsen, Kirsten Jochumsen, Claus Høgdall, Finn Lauszus, Estrid Høgdall

“…<h4>Background</h4>Identifying and describing molecular alterations in tumors has become common with the development of high-throughput sequencing. However, DNA sequencing in rare tumors, such as ovarian adult granulosa cell tumor (aGCT), often lacks statistical power due to the limited number of cases in each study. …”
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Whole exome analysis of primary immunodeficiency by E. S. Rahmani, Н. Azarpara, M. Karimipoor, Н. Rahimi

“…However, thanks to advancing in the DNA sequencing method and availability of sophisticated sequencers molecular characterization of genetic disorders have been revolutionized. …”
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Development of a high-throughput screening system for identification of novel reagents regulating DNA damage in human dermal fibroblasts by Bae Seunghee, An In-Sook, An Sungkwan

“…In the second step, we validated certain effective reagents identified in the first step by analyzing the cell cycle, evaluating cell death, and performing HPRT-DNA sequencing in DREAM-F cells treated with these reagents and UVB. …”
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Singleton-based species names and fungal rarity: Does the number really matter? by Jonathan Cazabonne, Allison K. Walker, Jonathan Lesven, Danny Haelewaters

“…This number is expected to be refined, especially with increasing knowledge about microfungi in undersampled habitats and increasing amounts of data derived from environmental DNA sequencing. A significant proportion of newly generated sequences fail to match with already named species, and thus represent what has been referred to as fungal “dark taxa”. …”
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From DNA to sustainability: the genomics approach in livestock production by Juan Carlos Gutiérrez-Añez

“…In this sense, genomic selection uses information from many genetic markers or single nucleotide polymorphisms (SNPs) in conjunction with measurements (phenotypes) of essential traits in livestock to improve selection accuracy, reduce the generation intervals, and increase genetic gains since the observation can be read and estimated with a DNA sequencing before the population express the character even before the animal is born. …”
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A novel Arabidopsis phyllosphere resident Protomyces species and a re-examination of genus Protomyces based on genome sequence data by Kai Wang, Timo Sipilä, Kirk Overmyer

“…Nuclear gene phylogenetic marker analysis suggests actin1 gene DNA sequences could be used with nuclear ribosomal DNA internal transcribed spacer sequences for rapid identification of Protomyces species. …”
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Chrysosporium queenslandicum: a potent keratinophilic fungus for keratinous waste degradation by Tarun Kumar Kumawat, Anima Sharma, Seema Bhadauria

“…This chicken feather degrading fungal strain previously isolated and identified by morphological and 18 s rDNA sequencing in laboratory, was used in the present study. …”
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Leishmania major Infection in Synanthropic Rodents: Evidence for the Urbanization of Zoonotic Cutaneous Leishmaniasis (ZCL) in Southern Iran by Saeed Shahabi, Kourosh Azizi, Qasem Asgari, Bahador Sarkari

“…Leishmania DNA was detected in 18 (43.9%) rodents, including 11 R. rattus, 6 M. musculus, and one M. persicus, based on DNA sequencing of the ITS2 gene and PCR of the kDNA. Phylogenetic reconstruction revealed that the parasite infecting the rodents was L. major. …”
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Analysis of engineered T7 bacteriophages containing genetic sequences encoding antimicrobial peptides by Tobias Ludwig, Daniela Volke, Andor Krizsan

“…The integration of DNA sequences derived from CRAMP and melittin into the phage genome resulted in the created T7Select-(M)CRAMP and T7Select-(M)melittin phages, which showed a lysis behavior like the phage without insert and partially inhibited the growth of potentially phage-resistant E. coli strains after the phage-mediated lysis.…”
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Two Novel 30K Proteins Overexpressed in Baculovirus System and Their Antiapoptotic Effect in Insect and Mammalian Cells by Wei Yu, Qing Li, Yuhua Yao, Yanping Quan, Yaozhou Zhang

“…Additionally, the cDNA sequences of both Slp and Lsp-t were obtained from the fat bodies of silkworm larvae by reverse transcription polymerase chain reaction. …”
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Comparative analysis of microbial contamination in diesel fuels using MALDI-TOF MS by Agnieszka Ludwiczak, Tomasz Zieliński, Ewelina Sibińska, Grażyna Czeszewska-Rosiak, Michał Złoch, Joanna Rudnicka, Andrzej Tretyn, Paweł Pomastowski

“…The Zybio system identified 48% of all microorganisms at the species level, whereas the Bruker system identified only 33%. The 16 S rDNA sequencing confirmed proteomic identifications by the MALDI systems with high accuracy. …”
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COEX-Seq: Convert a Variety of Measurements of Gene Expression in RNA-Seq by Sang Cheol Kim, Donghyeon Yu, Seong Beom Cho

“…Next generation sequencing (NGS), a high-throughput DNA sequencing technology, is widely used for molecular biological studies. …”
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Electrochemical detection of human papillomavirus DNA based on an ultrasensitive electrochemical sensing platform using N-graphene paper by Jia Wang, Juelan Ye, Gang Li

“…This work addresses this critical need by developing an ultrasensitive electrochemical biosensing platform based on nitrogen-doped graphene paper electrodes for quantifying high-risk HPV16 DNA sequences. The N-graphene nanohybrid, fabricated via simple solvothermal treatment of biomass-derived graphene oxide, demonstrated significantly enhanced electrical and electrocatalytic properties compared to undoped graphene. …”
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A sedimentary ancient DNA perspective on human and carnivore persistence through the Late Pleistocene in El Mirón Cave, Spain by Pere Gelabert, Victoria Oberreiter, Lawrence Guy Straus, Manuel Ramón González Morales, Susanna Sawyer, Ana B. Marín-Arroyo, Jeanne Marie Geiling, Florian Exler, Florian Brueck, Stefan Franz, Fernanda Tenorio Cano, Sophie Szedlacsek, Evelyn Zelger, Michelle Hämmerle, Brina Zagorc, Alejandro Llanos-Lizcano, Olivia Cheronet, José-Miguel Tejero, Thomas Rattei, Stephan M. Kraemer, Ron Pinhasi

“…Finally, we recover three human mtDNA sequences from the Solutrean levels. These sequences, along with published data, suggest mtDNA haplogroup continuity in Iberia throughout the Solutrean/Last Glacial Maximum period.…”
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Synthesis, DNA-Binding, Anticancer Evaluation, and Molecular Docking Studies of Bishomoleptic and Trisheteroleptic Ru-Diimine Complexes Bearing 2-(2-Pyridyl)-quinoxaline by Sofia Balou, Athanasios Zarkadoulas, Maria Koukouvitaki, Luciano Marchiò, Eleni K. Efthimiadou, Christiana A. Mitsopoulou

“…The experimental data were confirmed by molecular docking studies, employing two different DNA sequences. Both complexes, 1 and 2, bind with DNA via a minor groove mode of binding. …”
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Infections Caused by Actinomyces neuii: A Case Series and Review of an Unusual Bacterium by Nathan Zelyas, Susan Gee, Barb Nilsson, Tracy Bennett, Robert Rennie

“…Isolates’ identities were confirmed by matrix-assisted laser desorption ionization time-of-flight mass spectrometry microbial identification system (MALDI-TOF MS MIS; bioMérieux) and/or DNA sequencing. Results. Six cases of A. neuii infection were identified. …”
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Identification of a new fish trypanosome from the large yellow croaker (Larimichthys crocea) and description of its impact on host pathology, blood biochemical parameters and immun... by Yang Xiaoao, Qi Pengzhi, Tao Zhen, Zhang Qingwei, Wang Yanjie, Zhu Denghui, Yan Xiaojun, Fu Peipei, Guo Baoying

“…The parasite had significant morphological differences from Trypanosoma epinepheli Su, Feng, Jiang, Guo, Liu & Xu, 2014 and Trypanosoma carassii (Mitrofanov) Doflein, 1901. The 18S rDNA sequences of the trypanosome from L. crocea had the highest homology (98.4%) with T. carassii. …”
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Sex disparities in the association between rare earth elements exposure and genetic mutation frequencies in lung cancer patients by Mengyuan Liu, Jiali Zhang, Xiaohong Duan, Qiming Zhou, Jing Chen, Siyao Liu, Junyan Su, Li Han, Fan Yang, Niansong Qian

“…A cohort of 53 LUAD patients underwent tumor DNA sequencing (1123 cancer-related genes) and plasma REE (lanthanum (La), cerium (Ce), praseodymium (Pr), neodymium (Nd), and yttrium (Y)) quantification. …”
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Pulmonary Bacterial Communities in Surgically Resected Noncystic Fibrosis Bronchiectasis Lungs Are Similar to Those in Cystic Fibrosis by Heather Maughan, Kristopher S. Cunningham, Pauline W. Wang, Yu Zhang, Marcelo Cypel, Cecilia Chaparro, D. Elizabeth Tullis, Thomas K. Waddell, Shaf Keshavjee, Mingyao Liu, David S. Guttman, David M. Hwang

“…Bacterial 16S rRNA gene libraries were constructed from lung tissue of 10 non-CF bronchiectasis and 21 CF patients, followed by DNA sequencing of isolates from each library. Community characteristics were analyzed and compared between the two groups. …”
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