Molecular, Biochemical, and Clinical Characterization of Thirteen Patients with Glycogen Storage Disease 1a in Malaysia by Siti Aishah Abdul Wahab, Yusnita Yakob, Mohd Khairul Nizam Mohd Khalid, Noraishah Ali, Huey Yin Leong, Lock Hock Ngu

“…The G6PC mutation analysis was performed by PCR-DNA sequencing. Results. Patients were presented with hepatomegaly (92%), hypoglycaemia (38%), poor weight gain (23%), and short stature (15%). …”
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<i>TFL1</i>-like genes in <i>Vigna unguiculata</i> (L.) Walp. with different growth habit types by E. A. Krylova, E. K. Khlestkina

“…Using the Sanger DNA sequencing method, the primary structure of TFL1-like genes was studied in six cowpea accessions with different growth habit types and architectonics.Results. …”
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Isolation and characterization of cadmium-resistant Bacillus cereus strains from Cd-contaminated mining areas for potential bioremediation applications by Bixia Liang, Yimeng Feng, Xiyue Ji, Chune Li, Qian Li, Zhenshun Zeng, Yuqi Wang

“…Morphological analysis and 16S rDNA sequencing identified both strains as Bacillus cereus. …”
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Diversity and composition changes of intestinal fungi in patients with chronic kidney disease by XIE Huichao, XIAO Weidong, HAN Ben

“…Fresh fecal samples were collected from the CKD patients and healthy controls. ITS DNA sequencing was employed to determine the composition of intestinal fungi， and then bioinformatics analysis was applied to compare the differences in fungal community diversity， structure， and function between the 2 groups. …”
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Purification, Characterization, and Biocatalytic and Antibiofilm Activity of a Novel Dextranase from Talaromyces sp. by Mahasen Mohamed Ahmed Ebaya, Mohammed El-Mowafy, Mohamed Mohamed Adel El-Sokkary, Ramadan Hassan

“…By using the standard ITS rDNA sequencing analysis, the isolated strain was identified as Talaromyces sp. …”
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Tropical fruit-derived Lactiplantibacillus as potential probiotic and antifungal agents against Fusarium oxysporum by R. Vasundaradevi, M. Sarvajith, S. Divyashree, N. Deepa, Premila N. Achar, M. Y. Sreenivasa

“…Two strains isolated from Ficus racemosa demonstrated significant antifungal activity against Fusarium oxysporum. 16S rDNA sequencing identified these strains as Lactiplantibacillus plantarum MYSVCF3 and Lpb. argentoratensis MYSVCF5. …”
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Clinical Report of a 17q12 Microdeletion with Additionally Unreported Clinical Features by Jennifer L. Roberts, Stephanie K. Gandomi, Melissa Parra, Ira Lu, Chia-Ling Gau, Majed Dasouki, Merlin G. Butler

“…Since syndrome-specific DNA sequencing studies failed to provide an explanation for this patient’s unusual habitus, we postulate that this case represents an expansion of the 17q12 microdeletion phenotype. …”
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Problems with studying directional natural selection in humans by S. V. Mikhailova

“…These include bioinformatic analysis of DNA sequences via detection of linkage disequilibrium and of deviations from the random distribution of frequencies of genetic variants, demographic and anthropometric studies based on a search for a correlation between fertility and phenotypic traits, genome-wide association studies on fertility along with genetic loci and polygenic risk scores, and a comparison of allele frequencies between generations (in modern samples and in those obtained from burials). …”
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Three Novel Mutations in the NPHS1 Gene in Vietnamese Patients with Congenital Nephrotic Syndrome by Thi Kim Lien Nguyen, Van Dem Pham, Thu Huong Nguyen, Trung Kien Pham, Thi Quynh Huong Nguyen, Huy Hoang Nguyen

“…All 29 exons and exon-intron boundaries of NPHS1 were analyzed by PCR and DNA sequencing. Genetic analysis of the NPHS1 gene revealed one compound heterozygous variant p.Glu117Lys, one heterozygous missense mutation p.Asp310Asn, and one heterozygous frame-shifting mutation (c.3250_3251insG causing p.Val1084Glyfs⁎12) in patient 1. …”
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Phylogeography and hybridization of corvid birds in the Palearctic Region by A. P. Kryukov

“…Several genetic markers were unable to discriminate between pure carrion and hooded crows. Mitochondrial DNA sequences revealed no difference between carrion and hooded crows, but instead two diverged haplogroups within the eastern part of the distribution range of the carrion crow. …”
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Effects of Salmonella Typhimurium infection on intestinal flora and intestinal tissue arachidonic acid metabolism in Wenchang chickens by Shenghong Chen, Yaochen Xie, Dingqian Guo, Tiansen Li, Zhen Tan, Xuhua Ran, Xiaobo Wen

“…Typhimurium using 16s rDNA sequencing and targeted metabolomics. The results showed that the levels of ARA metabolites were increased in the cecum tissue of Wenchang chickens after infection with S. …”
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Unveiling early-life microbial colonization profile through characterizing low-biomass maternal-infant microbiomes by 2bRAD-M by Shuwen Hou, Yuesong Jiang, Feng Zhang, Tianfan Cheng, Dan Zhao, Jilong Yao, Ping Wen, Ping Wen, Lijian Jin, Shi Huang

“…However, analyzing these samples often faces technical challenges and limitations of low-resolution using conventional approaches due to their low microbial biomass.MethodsHere, we employed the type IIB restriction enzymes site-associated DNA sequencing for microbiome (2bRAD-M) as a reduced metagenomics method to address these issues and profile species-level microbial composition. …”
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Cecropin AD ameliorates pneumonia and intestinal injury in mice with mycoplasma pneumoniae by mediating gut microbiota by Bowen Li, Mingming Liu, Wenjing Du, Shuaidong Wang, Zekang Xu, Xiaoqian Zhang, Yang Zhang, Song Hua

“…Additionally, there was a notable upsurge in inflammatory factors within the affected tissues. 16 S rDNA sequencing revealed alterations in the colonic microbiota, including a decrease in the abundance of beneficial bacteria such as Corynebacterium_glutamicum and Candidatus_Saccharimonas, and an increase in the abundance of potential pathogens like Lachnospiraceae_NK4A136_group and Escherichia-Shigella. …”
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‘Two in One’ Cloning Vector Applied for Blunt-End and T-A Cloning with One-Step Digestion–Ligation and Screening of Positive Recombinants by Unaided Eyes by Xingli Zhang, Chong Teng, Kaidi Lyu, Shanhua Lyu, Yinglun Fan

“…To clone DNA sequences quickly and precisely into plasmids is essential for molecular biology studies. …”
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Molecular Sex Identification in the Hardy Rubber Tree (Eucommia ulmoides Oliver) via ddRAD Markers by Wencai Wang, Guoqian Yang, Xin Deng, Fengqing Shao, Yongquan Li, Wei Guo, Hong Liang, Xianzhi Zhang

“…In this study, double-digest restriction site-associated DNA sequencing (ddRAD-seq) was applied to screen sex-linked molecular markers for sex identification and investigation of the sex determination system in 20 male and female E. ulmoides individual plants, respectively. …”
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Temporal transcriptional profiling of host cells infected by a veterinary alphaherpesvirus using nanopore sequencing by Dóra Tombácz, Zoltán Maróti, Péter Oláh, Ákos Dörmő, Gábor Gulyás, Tibor Kalmár, Zsolt Csabai, Zsolt Boldogkői

“…Abstract In our research, we performed temporal transcriptomic profiling of host cells infected with Equid alphaherpesvirus 1 (EHV-1) by utilizing direct cDNA sequencing based on nanopore MinION technology. The sequencing reads were harnessed for transcript quantification at various time points. …”
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Expression of Sclerostin in Osteoporotic Fracture Patients Is Associated with DNA Methylation in the CpG Island of the SOST Gene by Yanming Cao, Bin Wang, Ding Wang, Dongxiang Zhan, Caiyuan Mai, Peng Wang, Qiushi Wei, Yamei Liu, Haibin Wang, Wei He, Liangliang Xu

“…The genomic DNA of non-OPF (non-osteoporotic fracture bone tissues) and OPF (osteoporotic fracture bone tissues) were treated by bisulfite modification, and methylation status of CpG sites in the CpG island of SOST gene promoter was determined by DNA sequencing. Results. SOST gene expression in the non-OPF group was lower than that in OPF group. …”
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Screening of Indigenous Oxalate Degrading Lactic Acid Bacteria from Human Faeces and South Indian Fermented Foods: Assessment of Probiotic Potential by Sivasamy Gomathi, Ponnusamy Sasikumar, Kolandaswamy Anbazhagan, Sundaresan Sasikumar, Murugan Kavitha, M. S. Selvi, Govindan Sadasivam Selvam

“…These were identified as Lactobacillus fermentum and Lactobacillus salivarius using 16S rDNA sequencing. Three strains, Lactobacillus fermentum TY5, Lactobacillus fermentum AB1, and Lactobacillus salivarius AB11, exhibited good adhesion to HT-29 cells and strong antimicrobial activity. …”
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Genetic Diversity and Structure of a Critically Endangered Ornamental Species, <i>Rhododendron farinosum</i>, with Extremely Small Populations by Fengjuan Li, Linyuan Fan, Jingli Zhang, Wen Liu, Wei Li, Yongpeng Ma, Hong Ma

“…To unravel the reasons behind the endangerment of this species and provide guidance for the rational conservation of this species, this study obtained a large number of SNP loci by using double-digest restriction-site-associated DNA sequencing (ddRAD-seq) to evaluate the genetic diversity and genetic structure of <i>R. farinosum</i>, as well as to infer the population history of this species. …”
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Genetic coupling of enhancer activity and connectivity in gene expression control by Helen Ray-Jones, Chak Kei Sung, Lai Ting Chan, Alexander Haglund, Pavel Artemov, Monica Della Rosa, Luminita Ruje, Frances Burden, Roman Kreuzhuber, Anna Litovskikh, Eline Weyenbergh, Zoï Brusselaers, Vanessa Xue Hui Tan, Mattia Frontini, Chris Wallace, Valeriya Malysheva, Leonardo Bottolo, Elena Vigorito, Mikhail Spivakov

“…Abstract Gene enhancers often form long-range contacts with promoters, but it remains unclear if the activity of enhancers and their chromosomal contacts are mediated by the same DNA sequences and recruited factors. Here, we study the effects of expression quantitative trait loci (eQTLs) on enhancer activity and promoter contacts in primary monocytes isolated from 34 male individuals. …”
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