Cellular Reprogramming, Genome Editing, and Alternative CRISPR Cas9 Technologies for Precise Gene Therapy of Duchenne Muscular Dystrophy by Peter Gee, Huaigeng Xu, Akitsu Hotta

“…In the past decade, the development of two innovative technologies, namely, induced pluripotent stem cells (iPSCs) and the CRISPR Cas9 system, has enabled researchers to model diseases derived from patient cells and precisely edit DNA sequences of interest, respectively. In particular, Duchenne muscular dystrophy (DMD) has been an exemplary monogenic disease model for combining these technologies to demonstrate that genome editing can correct genetic mutations in DMD patient-derived iPSCs. …”
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The southern house mosquito Culex quinquefasciatus in Abu Dhabi, UAE, is developing resistance to deltamethrin insecticide by Amgd sayed Ali, Asim Iqbal, Tamilarasan Kamalanathan, Antony Raj Gnanaprakasam, Sabu Shajahan, Mohammad Hamad Alsadeq, Mohamed Mahmood Al Marzooqi, Mohamad Hamdan, Mohammad Ali Al-Deeb

“…We screened Cx. quinquefasciatus adults (N = 174) for the presence of the mutation using allele-specific PCR (AS-PCR) and DNA sequencing. We detected the mutation and found the kdr allele in all the sampled locations. …”
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Unveiling pelagic-benthic coupling associated with the biological carbon pump in the Fram Strait (Arctic Ocean) by Simon Ramondenc, Damien Eveillard, Katja Metfies, Morten H. Iversen, Eva-Maria Nöthig, Dieter Piepenburg, Christiane Hasemann, Thomas Soltwedel

“…Looking at 15 years of eDNA sequences (18S-V4) from settling and sedimented organic matter in the Fram Strait, here we observe that most phylogenetic groups were transferred from pelagic to benthic ecosystems. …”
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THE COMPARATIVE MOLECULAR GENETICAL STUDY OF ASIAN POPULATIONS OF DROSOPHILA MERCATORUM (DIPTERA; DROSOPHILIDAE) ON THE COI GENE AND ITS1-ITS2 rRNA GENES FRAGMENTS by A. V. Ivannikov, O. V. Vaulin, Yu. A. Koromyslov, I. K. Zakharov

“…The variability of standard DNA sequences used for species identification (COI fragment and ITS1-ITS2 fragment of rRNA genes) was investigated in these specimens. …”
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XI International Mycological Congress: report of Congress action on nomenclature proposals relating to fungi by Tom W. May, Scott A. Redhead, Lorenzo Lombard, Amy Y. Rossman

“…The two Special-purpose Committees authorized were: “DNA Sequences as Types for Fungi” and “Names of Fungi with the Same Epithet”. …”
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Transplantation of fecal microbiota from low to high residual feed intake chickens: Impacts on RFI, microbial community and metabolites profiles by Chunlin Xie, Qiying Liang, Jiaheng Cheng, Yushan Yuan, Lu Xie, Jian Ji

“…Microbiota composition and functional profiles in LRFI and HFMT chickens formed a distinct cluster compared to HRFI chickens. Using 16S rDNA sequencing and RandomForest analysis, Slackia, Peptococcus, Blautia, and Dorea were identified as key microbial markers associated with feed efficiency. …”
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Species determination using AI machine-learning algorithms: Hebeloma as a case study by Peter Bartlett, Ursula Eberhardt, Nicole Schütz, Henry J. Beker

“…Over the last 20 years the authors have built a database of Hebeloma collections containing not only metadata but also parametrized morphological descriptions, where for about a third of the cases micromorphological characters have been analysed and are included, as well as DNA sequences for almost every collection. The database now has about 9000 collections including nearly every type collection worldwide and represents over 120 different taxa. …”
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Fitness landscapes of human microsatellites. by Ryan J Haasl, Bret A Payseur

“…Advances in DNA sequencing technology and computation now enable genome-wide scans for natural selection to be conducted on unprecedented scales. …”
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Recent Advances in DNA Systems for In Situ Telomerase Activity Detection and Imaging by Shiyi Zhang, Wenjing Xiong, Shuyue Xu, Ruocan Qian

“…They consist of repetitive DNA sequences that protect chromosome ends and regulate cell division. …”
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The mutational landscape of ARMC5 in Primary Bilateral Macronodular Adrenal Hyperplasia: an update by Lucas Bouys, Anna Vaczlavik, Isadora P. Cavalcante, Florian Violon, Anne Jouinot, Annabel Berthon, Patricia Vaduva, Stéphanie Espiard, Karine Perlemoine, Peter Kamenicky, Marie-Christine Vantyghem, Antoine Tabarin, Gérald Raverot, Cristina L. Ronchi, Ulrich Dischinger, Martin Reincke, Maria C. Fragoso, Constantine A. Stratakis, Albain Chansavang, Eric Pasmant, Bruno Ragazzon, Jérôme Bertherat, for the COMETE and ENSAT Networks

“…The pathogenicity of ARMC5 variants is established on the basis of their frequency in the general population, in silico predictions, familial segregation and tumor DNA sequencing. Conclusions This is the first extensive review of ARMC5 pathogenic variants. …”
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EFFECT OF FLANKING SEQUENCES ON THE ACCURACY OF THE RECOGNITION OF TRANSCRIPTION FACTOR BINDING SITES by T. M. Khlebodarova, D. Yu. Oshchepkov, V. G. Levitsky, O. A. Podkolodnaya, E. V. Ignatieva, E. A. Ananko, I. L. Stepanenko, N. A. Kolchanov

“…The optimum lengths of DNA sequences including natural binding sites, at which they are recognized most accurately, were estimated by the weight matrix method. …”
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Endometrial Cancer and Hypermethylation: Regulation of DNA and MicroRNA by Epigenetics by Kouji Banno, Iori Kisu, Megumi Yanokura, Kenta Masuda, Yusuke Kobayashi, Arisa Ueki, Kosuke Tsuji, Wataru Yamagami, Hiroyuki Nomura, Nobuyuki Susumu, Daisuke Aoki

“…Therefore, in recent years, epigenetic mechanisms that do not involve changes in DNA sequences have been examined. Studies aimed at detection of aberrant DNA hypermethylation in cancer cells present in microscopic amounts in vivo and application of the results to cancer diagnosis have also started. …”
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Apolipoprotein C-II Deposition Amyloidosis: A Potential Misdiagnosis as Light Chain Amyloidosis by Sadichhya Lohani, Emily Schuiteman, Lohit Garg, Dhiraj Yadav, Sami Zarouk

“…Further evaluation of biopsy specimens with laser microdissection and mass spectrometry and DNA sequencing of exons should be done routinely in patients with amyloidoses for definitive diagnosis. …”
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Interleukin-16 Polymorphism Is Associated with an Increased Risk of Ischemic Stroke by Xiao-li Liu, Jian-zong Du, Yu-miao Zhou, Qin-fen Shu, Ya-guo Li

“…A total of 198 patients with ischemic stroke and 236 controls were genotyped using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and DNA sequencing method. We found that the rs11556218TG genotype and G allele of IL-16 were associated with significantly increased risks of ischemic stroke (TG versus TT, adjusted OR = 1.88; 95% CI, 1.15–3.07; G versus T, adjusted OR = 1.54; 95% CI, 1.05–2.27, resp.). …”
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Isolation and identification of fungi found in contaminated fermented milk and antifungal activity of vanillin by Xu Pei, Mekonen Tekliye, Mingsheng Dong

“…Fungal isolates obtained were characterized by DNA sequencing of the internal transcribed spacer (ITS) region. …”
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Diaporthaceae associated with root and crown rot of maize by S.C. Lamprecht, P.W. Crous, J.Z. Groenewald, Y.T. Tewoldemedhin, W.F.O. Marasas

“…Furthermore, to clarify the higher-level phylogeny of these fungal genera, isolates were subjected to DNA sequencing of the nuclear ribosomal DNA (ITS & LSU). …”
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Phylogeny of Macrobrachium spp. (Decapoda, Pleocyemata) from Peru based on mitochondrial and nuclear data reveals a species complex comprising M. digueti (Bouvier, 1895) and M. tra... by Eliana Zelada-Mázmela, Lorenzo E. Reyes-Flores, Luis De Stefano-Beltrán

“…This study represents the first comprehensive phylogenetic analyses of Macrobrachium species from Peru, and contributes the first publicly available DNA sequences for M. inca and M. gallus, as well as the first sequences of M. americanum, M. panamense, M. digueti, and M. transandicum collected from Peruvian rivers.…”
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Vitamin D Receptor Gene Polymorphisms Influence T1D Susceptibility among Pakistanis by Maryam Mukhtar, Andleeb Batool, Abdul Wajid, Iram Qayyum

“…Genotypes were identified by DNA sequencing and PCR-RFLP. Results. The allelic and genotypic frequencies of FokΙ and ApaI were significantly associated with T1D (p<0.001) development. …”
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DNA alterations in ovarian adult granulosa cell tumours: A scoping review protocol. by Sven Karstensen, Karsten Kaiser, Caroline Moos, Tim Svenstrup Poulsen, Kirsten Jochumsen, Claus Høgdall, Finn Lauszus, Estrid Høgdall

“…<h4>Background</h4>Identifying and describing molecular alterations in tumors has become common with the development of high-throughput sequencing. However, DNA sequencing in rare tumors, such as ovarian adult granulosa cell tumor (aGCT), often lacks statistical power due to the limited number of cases in each study. …”
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Whole exome analysis of primary immunodeficiency by E. S. Rahmani, Н. Azarpara, M. Karimipoor, Н. Rahimi

“…However, thanks to advancing in the DNA sequencing method and availability of sophisticated sequencers molecular characterization of genetic disorders have been revolutionized. …”
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