Reanalysis of sequences of alleged Javan tiger highlights the difficulties in studying big cats and the need for high-throughput sequencing by Anubhab Khan, Yulianto Yulianto, Sabhrina Gita Aninta, Wirdateti Wirdateti

“…However, there are several difficulties in using genetic tools for big cat conservation that could be resolved by modern methods of DNA sequencing. Recent reports of the sighting of a putative Javan tiger Panthera tigris sondaica in West Java, Indonesia, highlight some of the difficulties of studying the genetics of big cats. …”
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Russian Science Foundation grant No. 20-14-00140 supported this study. The authors are thankful to the multi-access Center “Bioinformatics” for the use of computational resources a... by O. V. Vishnevsky, I. V. Chadaeva, E. B. Sharypova, B. M. Khandaev, K. A. Zolotareva, A. V. Kazachek, P. M. Ponomarenko, N. L. Podkolodny, D. A. Rasskazov, E. V. Zemlyanskaya, A. G. Bogomolov, O. A. Podkolodnaya, L. K. Savinkova, M. P. Ponomarenko

“…Our previous studies presented our publicly available Web service Plant_SNP_TATA_Z­tester, which makes it possible to estimate the equilibrium dissociation constant (KD) of TBP complexes with plant proximal promoters (as output data) using 90 bp of their DNA sequences (as input data). In this work, by means of this bioinformatics tool, 363 gene promoter DNA sequences representing 43 plant species were analyzed. …”
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Genotype-Phenotype Correlation of G6PD Mutations among Central Thai Children with G6PD Deficiency by Boonchai Boonyawat, Tim Phetthong, Nithipun Suksumek, Chanchai Traivaree

“…Our study demonstrated the efficiency of direct DNA sequencing which can identify 12 missense mutations in Thai children.…”
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Identification, distribution, and hosts of Meloidogyne spp. infecting horticultural crops in Florida, USA with focus on Meloidogyne enterolobii by Riva Gabrieli, Brito Janete A., de Oliveira Clemen, Marin Marcus, Gu Mengyi, Bui Hung Xuan, Desaeger Johan

“…Phylogenetic analysis showed low genetic variability among DNA sequences of M. enterolobii populations from Florida, other states in the USA, and other countries. …”
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Brigatinib treatment in a patient with advanced NSCLC with XPO1-ALK fusion: a case report by Yang Zhang, Ke-jie Li, Can Wang, Chang-lin Zou, Meng Su

“…In this case report, we provide evidence of a novel ALK fusion, XPO1-ALK (intergenic), identified by next-generation DNA sequencing in a patient with advanced lung cancer. …”
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Keiferia azapaensis sp. nov., the first representative of the New World micromoth genus Keiferia Busck (Lepidoptera, Gelechiidae) associated with a member of Asteraceae by Héctor Vargas

“…Despite this unusual host plant, a Maximum Likelihood analysis, based on mitochondrial DNA sequences, placed the new species within a well-supported Keiferia clade. …”
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The evolution of personalized medicine: literature review by I. S. Dolgopolov, M. Yu. Rykov

“…This belief was to somehow confirmed by the use of the latest technologies, such as DNA sequencing, proteomics, imaging protocols and the use of wireless devices for health monitoring, which revealed inter-individual differences in gene expression and penetrance levels. …”
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Morphological and Molecular studies of Acanthobra marmid Heckel, 1843 (Piscies, Cypriniformes, Leuciscidae) from the Middle of Iraq by Hind Dyia Hadi, Abed Hassan Baraaj, Atheer Hussein Ali

“…The barcoding result (DNA sequencing) in fishes found in the same family  (Leuciscidae) showed that it fit with A. marmid. …”
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Practical Tips for Construction of Custom Peptide Libraries and Affinity Selection by Using Commercially Available Phage Display Cloning Systems by Keisuke Fukunaga, Masumi Taki

“…In both systems, peptides containing odd number(s) of Cys should be designed with caution. Also, DNA sequencing of a constructed library before biopanning is highly recommended for finding unexpected bias.…”
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Progression from Sustained BK Viruria to Sustained BK Viremia with Immunosuppression Reduction Is Not Associated with Changes in the Noncoding Control Region of the BK Virus Genome by Imran A. Memon, Bijal A. Parikh, Monique Gaudreault-Keener, Rebecca Skelton, Gregory A. Storch, Daniel C. Brennan

“…The antimetabolite was withdrawn for BK viremia and reduction of the calcineurin inhibitor for sustained BK viremia. DNA sequencing from the 11 patients with sustained viremia revealed 8 insertions, 16 transversions, 3 deletions, and 17 transitions. …”
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Investigation and Identification of Fungal Diseases of <i>Aloe barbadensis</i> in China by Guohui Zhang, Qingjia Wan, Xiangyang Li, Jie Deng

“…The pathogenic fungi of wild and cultivated species of <i>A. barbadensis</i> were isolated by a tissue separation method, and DNA sequencing was carried out by using the sequence analysis of the ribosomal rDNA-ITS region, and the pathogenic fungi were classified and identified by finally combining morphological observations. …”
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Is the Goat a New Host for the G3 Indian Buffalo Strain of Echinococcus granulosus? by Pietro Calderini, Simona Gabrielli, Gabriella Cancrini

“…Specific PCR followed by DNA sequencing of the mitochondrial cox1 gene revealed for all the isolates 99.5% identity to the reference strain G3 genotype and 99.3% and 99.1% to G2 and G1, respectively. …”
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Detection of Quadruplex DNA by Gold Nanoparticles by Heather F. Crouse, Alex Doudt, Cassie Zerbe, Swarna Basu

“…Enhancement of PRLS signals greater than 50% was observed at nanomolar DNA concentration, and a lower limit of detection of 2.1 nM was established for three different quadruplex DNA sequences, including the thrombin-inhibiting single-stranded 15 mer aptamer DNA, d(GGTTGGTGTGGTTGG), and the double-stranded 12 mer DNA, d(G4T4G4). …”
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Assessing the Impact of Aldose Reductase (ALR2) Regulatory Gene Polymorphism in Diabetic Retinopathy Patients by Hadiza Rabe Musa, Amina Rabe Musa, Yahuza Gimba Muhammed, Usman Salisu Batagarawa, Aminu Ibrahim

“…This followed the successful amplification of the specified AR2 gene DNA segment through a Polymerase Chain Reaction (PCR) methodology. Subsequently, DNA sequencing was performed using the ABI Prism BigDyeTM Terminator Cycle Sequencing Ready Reaction Kit on the ABI PrismTM 3730/3730XL DNA Sequencer. …”
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Cloning, Structural Characterization, and Phylogenetic Analysis of Flower MADS-Box Genes from Crocus (Crocus sativus L.) by Athanasios S. Tsaftaris, Alexios N. Polidoros, Konstantinos Pasentsis, Apostolos Kalivas

“…In order to uncover and understand the molecular mechanisms controlling flower development in cultivated crocus and its relative wild progenitor species, and characterize a number of crocus flower mutants, we have cloned and characterized different, full-length, cDNA sequences encoding MADS-box transcription factor proteins involved in flower formation.…”
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Eurotium Cristatum Postfermentation of Fireweed and Apple Tree Leaf Herbal Teas by Tatiana A. Efimenko, Elena F. Shanenko, Tatiana G. Mukhamedzhanova, Olga V. Efremenkova, Yuriy A. Nikolayev, Elena N. Bilanenko, Marina V. Gernet, Artem G. Grishin, Ivan N. Serykh, Sergey V. Shevelev, Byazilya F. Vasilyeva, Svetlana N. Filippova, Galina I. El-Registan

“…The species identification was carried out based on morphocultural characteristics and DNA sequencing. This study is aimed at determining the feasibility of making postfermented herbal teas using E. cristatum and to evaluate their quality. …”
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Engineering CRISPR for emerging therapeutic applications by Xiaoqi Sun, Zhuang Liu

“…The discovery of the ability of CRISPR–Cas9 to cut targeted DNA sequences at predetermined sites has introduced a new era of precision and flexibility in genome editing. …”
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Extracting and analyzing micronuclei from mouse two-cell embryos fertilized with freeze-dried spermatozoa by Ikue Shibasaki, Hinata Sugiyama, Yuko Kamada, Hiroaki Nagatomo, Daiyu Ito, Sayaka Wakayama, Masatoshi Ooga, Tsuyoshi Kasai, Takashi Kohda, Teruhiko Wakayama

“…By depolymerizing the cytoskeleton of blastomeres with cytochalasin B and colcemid, some micronuclei could be extracted from blastomeres of ACS embryos using a glass needle of a micromanipulator. DNA-sequencing results of each extracted micronucleus revealed that chromosomes in micronuclei were randomly selected, usually only one, and often contained a portion rather than the full length of the chromosome. …”
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Bioprospecting Saccharomyces cerevisiae in fruits from Amazonian region for beer brewing by Luan Reis Honorato da Silva, Flávia da Silva Fernandes, Jocélia Pinheiro Santos, Érica Simplício de Souza, Lívia Melo Carneiro, João Paulo Alves Silva, Jacqueline da Silva Batista, João Vicente Braga de Souza

“…Identification of the isolated strains was achieved through biochemical assays and ITS rDNA (Internal Transcribed Spacer ribosomal DNA), sequencing, with emphasis on determining their affiliation to S. cerevisiae and assessing phylogenetic ties. …”
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Microbial Metagenomes Across a Complete Phytoplankton Bloom Cycle: High-Resolution Sampling Every 4 Hours Over 22 Days by Brook L. Nunn, Emma Timmins-Schiffman, Miranda C. Mudge, Deanna L. Plubell, Gabriella Chebli, Julia Kubanek, Michael Riffle, William S. Noble, Elizabeth Harvey, Tasman A. Nunn, Tatiana Rynearson, Marcel Huntemann, Kurt LaButti, Brian Foster, Bryce Foster, Simon Roux, Krishnaveni Palaniappan, Supratim Mukherjee, T. B. K. Reddy, Chris Daum, Alex Copeland, I-Min A. Chen, Natalia N. Ivanova, Nikos C. Kyrpides, Tijana Glavina del Rio, Emiley A. Eloe-Fadrosh

“…Abstract In May and June of 2021, marine microbial samples were collected for DNA sequencing in East Sound, WA, USA every 4 hours for 22 days. …”
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