Practical Tips for Construction of Custom Peptide Libraries and Affinity Selection by Using Commercially Available Phage Display Cloning Systems by Keisuke Fukunaga, Masumi Taki

“…In both systems, peptides containing odd number(s) of Cys should be designed with caution. Also, DNA sequencing of a constructed library before biopanning is highly recommended for finding unexpected bias.…”
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Progression from Sustained BK Viruria to Sustained BK Viremia with Immunosuppression Reduction Is Not Associated with Changes in the Noncoding Control Region of the BK Virus Genome by Imran A. Memon, Bijal A. Parikh, Monique Gaudreault-Keener, Rebecca Skelton, Gregory A. Storch, Daniel C. Brennan

“…The antimetabolite was withdrawn for BK viremia and reduction of the calcineurin inhibitor for sustained BK viremia. DNA sequencing from the 11 patients with sustained viremia revealed 8 insertions, 16 transversions, 3 deletions, and 17 transitions. …”
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Investigation and Identification of Fungal Diseases of <i>Aloe barbadensis</i> in China by Guohui Zhang, Qingjia Wan, Xiangyang Li, Jie Deng

“…The pathogenic fungi of wild and cultivated species of <i>A. barbadensis</i> were isolated by a tissue separation method, and DNA sequencing was carried out by using the sequence analysis of the ribosomal rDNA-ITS region, and the pathogenic fungi were classified and identified by finally combining morphological observations. …”
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Is the Goat a New Host for the G3 Indian Buffalo Strain of Echinococcus granulosus? by Pietro Calderini, Simona Gabrielli, Gabriella Cancrini

“…Specific PCR followed by DNA sequencing of the mitochondrial cox1 gene revealed for all the isolates 99.5% identity to the reference strain G3 genotype and 99.3% and 99.1% to G2 and G1, respectively. …”
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Detection of Quadruplex DNA by Gold Nanoparticles by Heather F. Crouse, Alex Doudt, Cassie Zerbe, Swarna Basu

“…Enhancement of PRLS signals greater than 50% was observed at nanomolar DNA concentration, and a lower limit of detection of 2.1 nM was established for three different quadruplex DNA sequences, including the thrombin-inhibiting single-stranded 15 mer aptamer DNA, d(GGTTGGTGTGGTTGG), and the double-stranded 12 mer DNA, d(G4T4G4). …”
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Shivasia gen. nov. for the Australasian smut Ustilago solida that historically shifted through five different genera by Matthias Lutz, Kálmán Vánky, Marcin Piątek

“…The generic position of the enigmatic smut fungus Ustilago solida is evaluated applying molecular phylogenetic analyses using ITS and LSU rDNA sequences as well as light and scanning electron microscopical investigations of several collections of this species. …”
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Eye development under the control of SRp55/B52-mediated alternative splicing of eyeless. by Weronika Fic, François Juge, Johann Soret, Jamal Tazi

“…The long isoform has impaired ability to trigger formation of ectopic eyes and to bind efficiently Eyeless target DNA sequences in vitro. When over-produced in the eye imaginal disc, this isoform induces a small eye phenotype, whereas the isoform lacking the alternative exon triggers eye over-growth and strong disorganization. …”
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A Novel Method for Functional Annotation Prediction Based on Combination of Classification Methods by Jaehee Jung, Heung Ki Lee, Gangman Yi

“…NGS is a popular research method since high-throughput technologies such as DNA sequencing and microarrays have created large sets of genes. …”
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Some Effective Tight-Binding Models for Electrons in DNA Conduction: A Review by Hiroaki Yamada, Kazumoto Iguchi

“…In addition, we investigated the localization properties of electronic states in several actual DNA sequences such as bacteriophages of Escherichia coli, human-chromosome 22, compared with those of the artificial disordered sequences with correlation. …”
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Cloning, Structural Characterization, and Phylogenetic Analysis of Flower MADS-Box Genes from Crocus (Crocus sativus L.) by Athanasios S. Tsaftaris, Alexios N. Polidoros, Konstantinos Pasentsis, Apostolos Kalivas

“…In order to uncover and understand the molecular mechanisms controlling flower development in cultivated crocus and its relative wild progenitor species, and characterize a number of crocus flower mutants, we have cloned and characterized different, full-length, cDNA sequences encoding MADS-box transcription factor proteins involved in flower formation.…”
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Eurotium Cristatum Postfermentation of Fireweed and Apple Tree Leaf Herbal Teas by Tatiana A. Efimenko, Elena F. Shanenko, Tatiana G. Mukhamedzhanova, Olga V. Efremenkova, Yuriy A. Nikolayev, Elena N. Bilanenko, Marina V. Gernet, Artem G. Grishin, Ivan N. Serykh, Sergey V. Shevelev, Byazilya F. Vasilyeva, Svetlana N. Filippova, Galina I. El-Registan

“…The species identification was carried out based on morphocultural characteristics and DNA sequencing. This study is aimed at determining the feasibility of making postfermented herbal teas using E. cristatum and to evaluate their quality. …”
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Bioprospecting Saccharomyces cerevisiae in fruits from Amazonian region for beer brewing by Luan Reis Honorato da Silva, Flávia da Silva Fernandes, Jocélia Pinheiro Santos, Érica Simplício de Souza, Lívia Melo Carneiro, João Paulo Alves Silva, Jacqueline da Silva Batista, João Vicente Braga de Souza

“…Identification of the isolated strains was achieved through biochemical assays and ITS rDNA (Internal Transcribed Spacer ribosomal DNA), sequencing, with emphasis on determining their affiliation to S. cerevisiae and assessing phylogenetic ties. …”
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Microbial Metagenomes Across a Complete Phytoplankton Bloom Cycle: High-Resolution Sampling Every 4 Hours Over 22 Days by Brook L. Nunn, Emma Timmins-Schiffman, Miranda C. Mudge, Deanna L. Plubell, Gabriella Chebli, Julia Kubanek, Michael Riffle, William S. Noble, Elizabeth Harvey, Tasman A. Nunn, Tatiana Rynearson, Marcel Huntemann, Kurt LaButti, Brian Foster, Bryce Foster, Simon Roux, Krishnaveni Palaniappan, Supratim Mukherjee, T. B. K. Reddy, Chris Daum, Alex Copeland, I-Min A. Chen, Natalia N. Ivanova, Nikos C. Kyrpides, Tijana Glavina del Rio, Emiley A. Eloe-Fadrosh

“…Abstract In May and June of 2021, marine microbial samples were collected for DNA sequencing in East Sound, WA, USA every 4 hours for 22 days. …”
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Prevalence of Drug Resistance and Associated Mutations in a Population of HIV-1+ Puerto Ricans: 2006–2010 by Lycely del C. Sepúlveda-Torres, Alexandra De La Rosa, Luz Cumba, Nawal Boukli, Eddy Ríos-Olivares, Luis A. Cubano

“…Samples from 2,500 patients from 2006–2010 were analyzed using the TruGene HIV-1 genotyping kit and the OpenGene DNA sequencing system. Results show that 58.8% of males and 65.3% of females had HIV-1 with resistance to at least one medication. …”
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Design and structure of overlapping regions in PCA via deep learning by Yan Zheng, Xi-Chen Cui, Fei Guo, Ming-Liang Dou, Ze-Xiong Xie, Ying-Jin Yuan

“…However, there still exists DNA sequences that are challenging to design and construct in the genome synthesis. …”
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Transcriptomic analysis of Andrias davidianus meat and experimental validation for exploring its bioactive components as functional foods by Changge Guan, Zhenglin Tan, Shucheng Li, Yi Wang, Naoyuki Yamamoto, Chong Zhang, Songjun Wang, Junjie Chen, Xin-Hui Xing

“…In this study, we illustrated full-length transcriptome sequencing to interpret the proteome of CGS meat and obtain 10703 coding DNA sequences. By functional annotation and amino acid composition analysis, we have discovered various genes related to signal transduction, and 16 genes related to longevity. …”
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ANALYSIS OF POLYMORPHISM OF ORGANELLE DNA TO ELUCIDATE THE PHYLOGEOGRAPHY OF NORWAY SPRUCE IN THE EAST EUROPEAN PLAIN by E. K. Potokina, A. A. Kiseleva, M. A. Nikolaeva, S. A. Ivanov, P. S. Ulianich, A. F. Potokin

“…Among the trees analyzed, some had chloroplast DNA sequences (trnT-trnF) assigned to P. abies, others carried cpDNA haplotypes fixed for P. obovata. …”
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Changes in microbiome composition during ontogeny and dispersal of the coral boring sponge Thoosa mismalolli by Eric Bautista-Guerrero, José Luis Carballo, Fabián Alejandro Rodríguez Zaragoza, Bruno Goméz-Gil, Alejandra García-Gasca, Alma P. Rodríguez-Troncoso, Adrián González-Castillo

“…We employed a combined approach of DNA sequencing and transmission electron microscopy to examine the changes in the microbiome during the ontogeny and dispersal of the coral-excavating sponge Thoosa mismalolli. …”
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New Hosts of Simplicimonas similis and Trichomitus batrachorum Identified by 18S Ribosomal RNA Gene Sequences by Kris Genelyn B. Dimasuay, Orlie John Y. Lavilla, Windell L. Rivera

“…Genomic DNA was extracted using Chelex method and the 18S rRNA gene was successfully amplified through novel sets of primers and undergone DNA sequencing. Aligned isolate sequences together with retrieved 18S rRNA gene sequences of known trichomonads were utilized to generate phylogenetic trees using maximum likelihood and neighbor-joining analyses. …”
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Heavy metal contamination and eutrophication effects on bacterial communities in the Han River basin by Fei Xiong, Cejia Yao, Dongdong Zhai, Hongyan Liu, Wang Dong, Yuanyuan Chen

“…This study analyzed microbial communities in sediment samples from 15 sites using 16S rDNA sequencing. Our findings revealed a significant decrease in α-diversity with increasing copper (Cu) concentration, with the downstream region (group L) exhibiting higher diversity compared to the upstream region (group U). …”
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