Fatal Hepatic Decompensation in a Patient with Hepatitis B Cirrhosis Following Famciclovir Withdrawal by Robert P Myers, Rabindra Chaudhary, Kevin Fonseca, Samuel S Lee

“…Although several mutations in the HBV DNA polymerase gene have been documented, none has been associated with famciclovir resistance or adverse clinical outcomes. …”
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Rare but Not so Rare? The Evolving Spectrum of Whipple's Disease by John M Conly, B Lynn Johnston

“…Once considered the ideal case report, recent characterization of Tropheryma whippelii by means of broad range bacterial ribosomal DNA polymerase chain reaction (PCR) analysis (2,3) and its subsequent cultivation (4) has led to a veritable explosion of individual case reports, case series and hitherto unrecognized manifestations of the disease, such that it is now considered an underdiagnosed infectious disease (5). …”
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Characterization of Esocid Herpesvirus 1 (EsHV1) from Europe by Mikael Leijon, Charlotte Axén, Fereshteh Banihashem, Tobias Lilja, Petter Tibblin, Björn David Persson

“…In addition, for the first time, full sequences of both the DNA polymerase and terminase of the virus is available, thus allowing for an improved phylogenetic placement of EsHV1 within the <i>Alloherpesviridae</i> family. …”
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Application of ectoine in diseases of the skin and mucous membranes by Piotr Kuna, Dominika Ochab-Krupnik, Anna Mościcka, Piotr Łacwik

“…It has been shown to have a positive effect on the stability of DNA helix, increases the temperature resistance of DNA polymerase, and lowers the melting temperature of the double-stranded DNA. …”
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A novel isothermal whole genome sequencing approach for Monkeypox Virus by Matthias Licheri, Manon Flore Licheri, Lukas Probst, Cora Sägesser, Pascal Bittel, Franziska Suter-Riniker, Ronald Dijkman

“…Here, we present the establishment of a novel isothermal WGS method for MPXV based on Phi29 DNA polymerase-based multiple displacement amplification (MDA) properties making use of only 6 primers. …”
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Rev1, Rev3, or Rev7 siRNA Abolishes Ultraviolet Light-Induced Translesion Replication in HeLa Cells: A Comprehensive Study Using Alkaline Sucrose Density Gradient Sedimentation by Jun Takezawa, Yukio Ishimi, Naomi Aiba, Kouichi Yamada

“…When a replicative DNA polymerase stalls upon encountering a lesion on the template strand, it is relieved by other low-processivity polymerase(s), which insert nucleotide(s) opposite the lesion, extend by a few nucleotides, and dissociate from the 3′-OH. …”
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Genotypic Profiling of Bacillus cereus Recovered from Some Retail Foods in Ogun State, Nigeria, and Their Phylogenetic Relationship by Titilayo O. Adesetan, Moses O. Efuntoye, Olubukola O. Babalola

“…Genotypic profiling of B. cereus isolates from food was obtained by Random Amplified Polymorphic DNA-polymerase chain reaction (RAPD-PCR) using OPR13 primer. …”
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Conserved Nuclear Localization Signal in NS2 Protein of Bombyx Mori Bidensovirus: A Potential Invertebrate ssDNA Virus Trait by Qian Yu, Jiaxin Yan, Ying Chen, Jinfeng Zhang, Qi Tang, Feifei Zhu, Lindan Sun, Shangshang Ma, Xiaoyong Liu, Keping Chen, Qin Yao

“…Bombyx mori bidensovirus (BmBDV), a significant pathogen in the sericulture industry, holds a unique taxonomic position due to its distinct segmented single-stranded DNA (ssDNA) genome and the presence of a self-encoding DNA polymerase. However, the functions of viral non-structural proteins, such as NS2, remain unknown. …”
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Evaluation of Procalcitonin, C-Reactive Protein, and Interleukin-6 as Early Markers for Diagnosis of Neonatal Sepsis by Emad A. Morad, Rehab A. Rabie, Mohamed A. Almalky, Manar G. Gebriel

“…Also, bacterial DNA was revealed from the blood by broad-range 16S rDNA polymerase chain reaction (PCR). Measurements of CRP using the immunoturbidimetry method, PCT using fluorescence immunoassay quantitative method, and IL-6 using commercially available ELISA kit were done to all enrolled neonates. …”
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Peptide nucleic acid (PNA) clamps reduce amplification of host chloroplast and mitochondria rRNA gene sequences and increase detected diversity in 16S rRNA gene profiling analysis... by Usman Hussain, Jim Downie, Amy Ellison, Sandra Denman, James McDonald, Marine C. Cambon

“…Peptide Nucleic Acid (PNA) clamps prevent this by blocking PCR primer binding or binding within the amplified region of non-target DNA to stop the function of DNA polymerase. Here, we applied a universal chloroplast (p)PNA clamp and a newly designed mitochondria (m)PNA clamp to minimise host chloroplast and mitochondria amplification in 16S rRNA gene amplicon profiles of leaf, bark and root tissue of two oak species (Quercus robur and Q. petraea). …”
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Expanding the Diversity of <i>Actinobacterial Tectiviridae</i>: A Novel Genus from <i>Microbacterium</i> by Jacqueline M. Washington, Holly Basta, Angela Bryanne De Jesus, Madison G. Bendele, Steven G. Cresawn, Emily K. Ginser

“…Like other <i>Tectiviridae</i>, the phage genomes are arranged into two segments and include three highly conserved family genes that encode a DNA polymerase, double jelly-roll major capsid protein, and packaging ATPase. …”
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Whole-genome amplification as a tool to improve bacterial detection by PCR in microbiological samples after endodontic treatment by Gabriela Ceccon Chianca, Gabriela Ceccon Chianca, Caroline Corrêa Fendeler, Caroline Corrêa Fendeler, Sérgio Pinto Chaves Junior, Sérgio Pinto Chaves Junior, Gabriella Lorena Dias Pereira, Helvécio Cardoso Corrêa Póvoa, Helvécio Cardoso Corrêa Póvoa, Helvécio Cardoso Corrêa Póvoa, Lívia Azeredo Alves Antunes, Lívia Azeredo Alves Antunes, Lívia Azeredo Alves Antunes, Leonardo Santos Antunes, Leonardo Santos Antunes, Leonardo Santos Antunes, Natalia Lopes Pontes Póvoa Iorio, Natalia Lopes Pontes Póvoa Iorio, Natalia Lopes Pontes Póvoa Iorio

“…Microbiological samples were collected before and after CMP using paper points, which were separated into two groups: (i) culture assay samples were plated onto Brucella agar with 5% defibrinated sheep's blood, menadione and hemin and incubated anaerobically for 14 days at 36°C; (ii) DNA was extracted from molecular assay samples and subject to WGA by isothermal strand displacement with Phi29 DNA polymerase followed by PCR to determine the presence of bacteria.ResultsIn both assays, samples before CMP showed the presence of bacteria in all 10 teeth. …”
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Genomic analysis and replication kinetics of the closely related EHV-1 neuropathogenic 21P40 and abortigenic 97P70 strains by Eslam Mohamed, Ines Zarak, Nick Vereecke, Sebastiaan Theuns, Kathlyn Laval, Hans Nauwynck

“…Isoleucine and asparagine at loci 291 and 207 of ORF30 (DNA polymerase) and ORF65 (ICP22), respectively, were unique to isolates from the Valencia outbreak. …”
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Efficient method for identification of Escherichia coli strains isolated from various chicken (Gallus gallus domesticus) organs by V. P. Terletskiy, V. I. Tyshchenko, O. B. Novikova, E. D. Dzhavadov, I. Ya. Shakhtamirov, N. L. Adaev

“…Bacterial genomic DNA is simultaneously digested with two restriction enzymes and labeled with biotinylated deoxynucleoside triphosphates with the presence of DNA polymerase. The enzymes are chosen in silico for each bacterial species so that a limited number of DNA fragments be generated for subsequent separation in conventional agarose gel. …”
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The clinical utility of tissue polymerase chain reaction, tissue culture and tissue histology in blood culture-negative infective endocarditis in South Africa – insights from the G... by W Endres, P Mkoko, M Ntsekhe

“…Among patients undergoing surgery for IE, valve tissue may be evaluated for additional microbiological information by performing a broad-range 16S rDNA polymerase chain reaction (PCR) test, tissue culture and tissue histopathology. …”
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Disproportionate Distribution of HBV Genotypes A and D and the Recombinant Genotype D/E in the High and Low HBV Endemic Regions of Uganda: A Wake-Up Call for Regional Specific HBV... by Hussein Mukasa Kafeero, Dorothy Ndagire, Ponsiano Ocama, Charles Drago Kato, Eddie Wampande, Henry Kajumbula, David Kateete, Abdul Walusansa, Ali Kudamba, Kigozi Edgar, Fred Ashaba Katabazi, Maria Magdalene Namaganda, Jamilu E. Ssenku, Hakim Sendagire

“…Nested PCR amplification was performed using Platinum Taq DNA Polymerase (Invitrogen Corporation, USA) to amplify the 400 bp HBV polymerase gene. …”
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