Building a reliable 16S mini-barcode library of wild bees from Occitania, south-west of France by Anaïs Marquisseau, Kamila Canale-Tabet, Emmanuelle Labarthe, Géraldine Pascal, Christophe Klopp, André Pornon, Nathalie Escaravage, Rémi Rudelle, Alain Vignal, Annie Ouin, Mélodie Ollivier, Magalie Pichon

“…DNA barcoding and metabarcoding are now powerful tools for studying biodiversity and especially the accurate identification of large sample collections belonging to diverse taxonomic groups. …”
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Historical biogeography and systematics of yellow-bellied toads (Bombina variegata), with the description of a new subspecies from the Balkans by Christophe Dufresnes, Simeon Lukanov, Sven Gippner, Johanna Ambu, Ilias Strachinis, Dragan Arsovski, Benjamin Monod-Broca, Hugo Cayuela, Petros Lymberakis, Daniele Canestrelli, Dan Cogălniceanu, Nikolay A. Poyarkov, Spartak N. Litvinchuk, Tomasz Suchan, Mathieu Denoël, Daniel Jablonski

“…In this study, we combine cytochrome b DNA barcoding data (1238 individuals from 355 localities), mitogenome phylogenetics (17.2 kb), gene-based nuclear phylogenetics (3.7 kb from four gene fragments) and multilocus phylogenomics (4759 loci / ~554 kb obtained by double digest Restriction Associated DNA sequencing; ddRAD-seq) to re-assess the diversification of B. variegata, and revisit its nomenclatural history to assign scientific names to phylogeographic lineages. …”
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Chemical Fingerprint Analysis for Discovering Markers and Identifying Saussurea involucrata by HPLC Coupled with OPLS-DA by Qingdong Ma, Xiaoxiang Chen, Ke Zhang, Dahong Yao, Lu Yang, Hangyu Wang, Santai Bulemasi, Jian Huang, Jinhui Wang

“…In this work, a comprehensive chromatographic fingerprint method was developed and tested on 43 samples from four haplotypes of S. involucrata according to DNA barcoding. Three common patterns consisting of 20, 14, and 7 common peaks were generated by frequency filters of median, upper quartile, and 100%, respectively. …”
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Identification of Kappaphycus alvarezii seaweed based on phylogenetic and carrageenan conten by Gloria Ika Satriani, Dinar Tri Soelistyowati, Alimuddin, Harton Arfah, Irzal Effendi

“…Penelitian ini bertujuan untuk memperoleh informasi filogenetik berdasarkan identifikasi sumber genetiknya menggunakan penanda molekuler DNA barcoding rbcL serta analisis kandungan karaginannya menggunakan spektrum Fourier Transform Infra-Red (FTIR). …”
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A multiplex method for rapidly identifying viral protease inhibitors by Seo Jung Hong, Samuel J Resnick, Sho Iketani, Ji Won Cha, Benjamin Alexander Albert, Christopher T Fazekas, Ching-Wen Chang, Hengrui Liu, Shlomi Dagan, Michael R Abagyan, Pavla Fajtová, Bruce Culbertson, Brooklyn Brace, Eswar R Reddem, Farhad Forouhar, J Fraser Glickman, James M Balkovec, Brent R Stockwell, Lawrence Shapiro, Anthony J O’Donoghue, Yosef Sabo, Joel S Freundlich, David D Ho, Alejandro Chavez

“…This multiplex approach is enabled by using cellular biosensors of viral protease activity combined with DNA-barcoding technology, as well as several design innovations that increase assay sensitivity and correct for plate-to-plate variation. …”
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Identification of Southeast Asian Anopheles mosquito species with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry using a cross-correlation approach by Victor Chaumeau, Sunisa Sawasdichai, Thu Zar Ma Ma Moe Min, Thithiwarada Kularbkeeree, Naw Jaruwan, Naw Gloria, Naw Yu Lee, Muesuwa Trackoolchengkaew, Monticha Phanaphadungtham, Patcharamai Rongthong, Aritsara Inta, Wanitda Watthanaworawit, François Nosten

“…Three hundred fifty-nine specimens could be identified with DNA barcodes and were assigned to 21 sensu stricto species and five sibling species pairs or complexes. …”
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Speciation and historical invasions of the Asian black-spined toad (Duttaphrynus melanostictus) by Christophe Dufresnes, Daniel Jablonski, Johanna Ambu, Vishal Kumar Prasad, Kumudani Bala Gautam, Rachunliu G. Kamei, Stephen Mahony, Sylvia Hofmann, Rafaqat Masroor, Bérénice Alard, Angelica Crottini, Devin Edmonds, Annemarie Ohler, Jianping Jiang, Janak R. Khatiwada, Sandeep Kumar Gupta, Amaël Borzée, Leo J. Borkin, Dmitriy V. Skorinov, Daniel A. Melnikov, Konstantin D. Milto, Evgeny L. Konstantinov, Sven Künzel, Tomasz Suchan, Dmitriy V. Arkhipov, Alexei V. Trofimets, Tan Van Nguyen, Chatmongkon Suwannapoom, Spartak N. Litvinchuk, Nikolay A. Poyarkov

“…Combining continental-wide genomic and DNA-barcoding analyses, we reconstructed the historical biogeography of the Asian black-spined toad (Duttaphrynus melanostictus), a toxic commensal amphibian that currently threatens two biodiversity hotspots through biological invasions (Wallacea and Madagascar). …”
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Characterization of CO1 and 18S rDNA genes from Indonesian native ornamental shrimp Caridina boehmei cultured in Jakarta by Ekky Ilham Romadhona, Novi Megawati, Handang Widantara, Sutanti Sutanti, Arif Rahman Ardiansyah, Kiki Mariya Dewi, Annisa Fitri Larassagita, Aditia Firman, Moh Risky, Rahmat Rahmat, Ayub Ayub

“…Kata kunci: 18S rDNA, Caridina boehmei, CO1, DNA barcode, udang hias …”
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First assessment of biofouling assemblages in the northern Red Sea, an important region for marine non-indigenous species transfer by Juan Sempere-Valverde, Juan Sempere-Valverde, Eva Aylagas, Vitaly Syomin, Vitaly Syomin, Marcos A. L. Teixeira, Glafira D. Kolbasova, Glafira D. Kolbasova, Sofía Ruiz-Velasco, Sahar Chebaane, Arthur Anker, João Curdia, Ronald Cadiz, Dylan M. Cottrell, Andrea Desiderato, Luiz F. Andrade, Chakkiath Paul Antony, Carolina Bocanegra-Castano, Matilde Marzucchi, Angelo Poliseno, Doaa Baker, Basmah Alabdulaziz, Flor Torres, Ameer A. Eweida, Ameer A. Eweida, Susana Carvalho, Susana Carvalho

“…Panels were analysed with photo quadrat analysis (PhQd), examined to manually collect sessile macroinvertebrate specimens for taxonomic identification using morphological and DNA barcoding analysis, and scraped for bulk DNA analyses using DNA metabarcoding. …”
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A new species of the genus Oiketicoides Heylaerts, 1885 (Lepidoptera, Psychidae) from Korea with its natural parasitoid enemy by Dong-June Lee, Jongwon Kim, Seung-Su Euo, Jae-Seok Lee, Hyeon Lee, Seung Jin Roh

“…The morphology of male adult, including genitalia, is described, and DNA barcodes for precise identification of the species are provided. …”
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Description of five new species from southern China, with note on the type species of Latouchia Pocock, 1901 (Araneae, Halonoproctidae) by Long Hao, Kun Yu, Feng Zhang

“…. (♂♀) from south part of Shaanxi. DNA barcodes of the new species described herein are provided. …”
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Three new species of Australian miracine parasitoid wasps collected by regional schools as part of the Insect Investigators citizen science project (Hymenoptera, Braconidae, Miraci... by Mollie-Rosae Slater-Baker, Michelle Guzik, Juanita Rodriguez, Andy Howe, Alice Woodward, Nathan Ducker, Erinn Fagan-Jeffries

“…The barcoding region of the cytochrome c oxidase subunit I (COI) gene was obtained for each new species and analysed alongside all publicly available COI DNA barcodes for Miracinae on the Barcode of Life Database, to produce a molecular framework to guide species delimitation and efficient morphological examination. …”
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On new spider species of the genus Episinus (Araneae, Theridiidae) from China and proposal of five species groups by Yun Liang, Jinnan Liu, Haiqiang Yin, Xiang Xu

“…Detailed descriptions, photographs, hand drawings, DNA barcodes and a distribution map of the three new species are provided.…”
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Barcoded screening identifies nanocarriers for protein delivery to kidney by Luyao Wang, Wen Zhou, Hang Chen, Xiangqian Jia, Peiyuan Zheng, Haolin Jiang, Mengling Wu, Yaning Zhang, Yanchao Ding, Yexi Peng, Rui Zhu, Tiantian Li, Boxue Tian, Bujie Du, Juanjuan Du

“…In this study, we address this challenge by leveraging DNA-barcoded high-throughput screening. We construct a nanocapsule library via in-situ polymerization, incorporating various monomers to create nanocapsules with unique surface properties. …”
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Study of endophytic fungi of Ipomoea pes-caprae reveals the superiority of in situ plant conservation over ex situ conservation from a mycological view by Yu-Hung Yeh, Roland Kirschner

“…In a culture-dependent approach, endophytic fungi were isolated under axenic conditions and identified to species, genus, or higher taxonomic ranks with DNA barcodes and morphology. In addition to yielding ca. 800 strains and over 100 morphospecies, a principal component analysis (PCA) of the distribution of the dominant fungal species showed clear differences in the composition of endophytic fungal species depending on the sampling sites. …”
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Species delimitation of newly collected spiders of the genus Pseudopoda (Araneae, Sparassidae) from Honghe Hani and Yi Autonomous Prefecture, Yunnan, China: An integrated morpholog... by Jianshuang Zhang, Tianqin Pan, He Zhang, Yuanqian Xing, Hao Yu, Yang Zhong

“…Detailed descriptions, diagnoses, photographs of the six species, and a distribution map for all 13 Pseudopoda species in Honghe Prefecture are provided. The DNA barcodes of the six species were obtained for species delimitation, sex matching, and future use.…”
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New banded Leporinus (Characiformes: Anostomidae) from the Madeira River basin, Brazil, and redescription of L. bleheri, based on integrative taxonomy by Mariana Pascoal Boaretto, Willian Massaharu Ohara, Lenice Souza-Shibatta, José Luís Olivan Birindelli

“…Both species specimens were cleared and double-stained for osteological description. DNA barcodes were used to investigate the genetic distance between samples and for species delimitation analyses. …”
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Deterministic genetic barcoding for multiplexed behavioral and single-cell transcriptomic studies by Jorge Blanco Mendana, Margaret Donovan, Lindsey Gengelbach O'Brien, Benjamin Auch, John Garbe, Daryl M Gohl

“…This method, called Targeted Genetically-Encoded Multiplexing (TaG-EM), involves inserting a DNA barcode just upstream of the polyadenylation site in a Gal4-inducible UAS-GFP construct so that the barcode sequence can be read out during single-cell sequencing, labeling a cell population of interest. …”
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Metatranscriptomics as a tool to identify fungal species and subspecies in mixed communities – a proof of concept under laboratory conditions by Vanesa R. Marcelino, Laszlo Irinyi, John-Sebastian Eden, Wieland Meyer, Edward C. Holmes, Tania C. Sorrell

“…Organisms in mixed microbial communities can now be sequenced and identified in a culture-independent way, usually using amplicon sequencing of a DNA barcode. Bulk RNA-seq (metatranscriptomics) has several advantages over DNA-based amplicon sequencing: it is less susceptible to amplification biases, it captures only living organisms, and it enables a larger set of genes to be used for taxonomic identification. …”
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Analysis of the transcriptional activity of model piggyBac transgenes stably integrated into different loci of the genome of CHO cells in the absence of selection pressure by L. A. Yarinich, A. A. Ogienko, A. V. Pindyurin, E. S. Omelina

“…Each individual transgene contained a unique tag, a DNA barcode, and the resulting polyclonal cell population was cultured for almost a month without any selection. …”
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