Chloroplast Genome Sequencing and Comparative Analysis of Six Medicinal Plants of Polygonatum by Jinchen Yao, Zhaohuan Zheng, Tao Xu, Duomei Wang, Jingzhe Pu, Yazhong Zhang, Liangping Zha

“…This study provides a novel basis for intragenus taxonomy and DNA barcoding molecular identification within the genus Polygonatum medicinal plants.…”
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Emergence of Fusarium incarnatum and Fusarium avenaceum in wilt affected solanaceous crops of the Northern Himalayas by Tasmeen J. Parihar, Madeeha Naik, Shafqat Mehraj, Syed Inam ul Haq, Maqsooda Perveen, Ishfaq Ahmed Malla, Taniya Abid, Nadia Gul, Khalid Z. Masoodi

“…Through both morphological and molecular analyses, including DNA barcoding of the ITS, TEF, RPB1, and RPB2 genomic regions, Fusarium incarnatum and Fusarium avenaceum were identified as the primary causal agents of wilt in tomato and brinjal, and chili, respectively. …”
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Identity and Distribution of <i>Triglops metopias</i> (Teleostei, Cottidae) in the Northwestern Pacific by Artem M. Prokofiev, Ilyas N. Mukhametov, Olga R. Emelianova, Svetlana Yu. Orlova, Alexei M. Orlov

“…Forty-one specimens were included in morphological and molecular analyses, including principal component analysis and DNA barcoding. The detailed morphological description of the Kuril Islands specimens is given. …”
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High-Throughput Oxford Nanopore Sequencing Unveils Complex Viral Population in Kansas Wheat: Implications for Sustainable Virus Management by Nar B. Ranabhat, John P. Fellers, Myron A. Bruce, Jessica L. Shoup Rupp

“…Wheat leaves exhibiting virus-like symptoms were collected, total RNA was extracted, and cDNA libraries were prepared using a PCR-cDNA barcoding kit, then loaded onto ONT MinION flow cells. …”
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Historical biogeography and systematics of yellow-bellied toads (Bombina variegata), with the description of a new subspecies from the Balkans by Christophe Dufresnes, Simeon Lukanov, Sven Gippner, Johanna Ambu, Ilias Strachinis, Dragan Arsovski, Benjamin Monod-Broca, Hugo Cayuela, Petros Lymberakis, Daniele Canestrelli, Dan Cogălniceanu, Nikolay A. Poyarkov, Spartak N. Litvinchuk, Tomasz Suchan, Mathieu Denoël, Daniel Jablonski

“…In this study, we combine cytochrome b DNA barcoding data (1238 individuals from 355 localities), mitogenome phylogenetics (17.2 kb), gene-based nuclear phylogenetics (3.7 kb from four gene fragments) and multilocus phylogenomics (4759 loci / ~554 kb obtained by double digest Restriction Associated DNA sequencing; ddRAD-seq) to re-assess the diversification of B. variegata, and revisit its nomenclatural history to assign scientific names to phylogeographic lineages. …”
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Chemical Fingerprint Analysis for Discovering Markers and Identifying Saussurea involucrata by HPLC Coupled with OPLS-DA by Qingdong Ma, Xiaoxiang Chen, Ke Zhang, Dahong Yao, Lu Yang, Hangyu Wang, Santai Bulemasi, Jian Huang, Jinhui Wang

“…In this work, a comprehensive chromatographic fingerprint method was developed and tested on 43 samples from four haplotypes of S. involucrata according to DNA barcoding. Three common patterns consisting of 20, 14, and 7 common peaks were generated by frequency filters of median, upper quartile, and 100%, respectively. …”
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Identification of Kappaphycus alvarezii seaweed based on phylogenetic and carrageenan conten by Gloria Ika Satriani, Dinar Tri Soelistyowati, Alimuddin, Harton Arfah, Irzal Effendi

“…Penelitian ini bertujuan untuk memperoleh informasi filogenetik berdasarkan identifikasi sumber genetiknya menggunakan penanda molekuler DNA barcoding rbcL serta analisis kandungan karaginannya menggunakan spektrum Fourier Transform Infra-Red (FTIR). …”
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A multiplex method for rapidly identifying viral protease inhibitors by Seo Jung Hong, Samuel J Resnick, Sho Iketani, Ji Won Cha, Benjamin Alexander Albert, Christopher T Fazekas, Ching-Wen Chang, Hengrui Liu, Shlomi Dagan, Michael R Abagyan, Pavla Fajtová, Bruce Culbertson, Brooklyn Brace, Eswar R Reddem, Farhad Forouhar, J Fraser Glickman, James M Balkovec, Brent R Stockwell, Lawrence Shapiro, Anthony J O’Donoghue, Yosef Sabo, Joel S Freundlich, David D Ho, Alejandro Chavez

“…This multiplex approach is enabled by using cellular biosensors of viral protease activity combined with DNA-barcoding technology, as well as several design innovations that increase assay sensitivity and correct for plate-to-plate variation. …”
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Identification of Southeast Asian Anopheles mosquito species with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry using a cross-correlation approach by Victor Chaumeau, Sunisa Sawasdichai, Thu Zar Ma Ma Moe Min, Thithiwarada Kularbkeeree, Naw Jaruwan, Naw Gloria, Naw Yu Lee, Muesuwa Trackoolchengkaew, Monticha Phanaphadungtham, Patcharamai Rongthong, Aritsara Inta, Wanitda Watthanaworawit, François Nosten

“…Three hundred fifty-nine specimens could be identified with DNA barcodes and were assigned to 21 sensu stricto species and five sibling species pairs or complexes. …”
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Characterization of CO1 and 18S rDNA genes from Indonesian native ornamental shrimp Caridina boehmei cultured in Jakarta by Ekky Ilham Romadhona, Novi Megawati, Handang Widantara, Sutanti Sutanti, Arif Rahman Ardiansyah, Kiki Mariya Dewi, Annisa Fitri Larassagita, Aditia Firman, Moh Risky, Rahmat Rahmat, Ayub Ayub

“…Kata kunci: 18S rDNA, Caridina boehmei, CO1, DNA barcode, udang hias …”
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First assessment of biofouling assemblages in the northern Red Sea, an important region for marine non-indigenous species transfer by Juan Sempere-Valverde, Juan Sempere-Valverde, Eva Aylagas, Vitaly Syomin, Vitaly Syomin, Marcos A. L. Teixeira, Glafira D. Kolbasova, Glafira D. Kolbasova, Sofía Ruiz-Velasco, Sahar Chebaane, Arthur Anker, João Curdia, Ronald Cadiz, Dylan M. Cottrell, Andrea Desiderato, Luiz F. Andrade, Chakkiath Paul Antony, Carolina Bocanegra-Castano, Matilde Marzucchi, Angelo Poliseno, Doaa Baker, Basmah Alabdulaziz, Flor Torres, Ameer A. Eweida, Ameer A. Eweida, Susana Carvalho, Susana Carvalho

“…Panels were analysed with photo quadrat analysis (PhQd), examined to manually collect sessile macroinvertebrate specimens for taxonomic identification using morphological and DNA barcoding analysis, and scraped for bulk DNA analyses using DNA metabarcoding. …”
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Three new species of Australian miracine parasitoid wasps collected by regional schools as part of the Insect Investigators citizen science project (Hymenoptera, Braconidae, Miraci... by Mollie-Rosae Slater-Baker, Michelle Guzik, Juanita Rodriguez, Andy Howe, Alice Woodward, Nathan Ducker, Erinn Fagan-Jeffries

“…The barcoding region of the cytochrome c oxidase subunit I (COI) gene was obtained for each new species and analysed alongside all publicly available COI DNA barcodes for Miracinae on the Barcode of Life Database, to produce a molecular framework to guide species delimitation and efficient morphological examination. …”
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On new spider species of the genus Episinus (Araneae, Theridiidae) from China and proposal of five species groups by Yun Liang, Jinnan Liu, Haiqiang Yin, Xiang Xu

“…Detailed descriptions, photographs, hand drawings, DNA barcodes and a distribution map of the three new species are provided.…”
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Barcoded screening identifies nanocarriers for protein delivery to kidney by Luyao Wang, Wen Zhou, Hang Chen, Xiangqian Jia, Peiyuan Zheng, Haolin Jiang, Mengling Wu, Yaning Zhang, Yanchao Ding, Yexi Peng, Rui Zhu, Tiantian Li, Boxue Tian, Bujie Du, Juanjuan Du

“…In this study, we address this challenge by leveraging DNA-barcoded high-throughput screening. We construct a nanocapsule library via in-situ polymerization, incorporating various monomers to create nanocapsules with unique surface properties. …”
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Study of endophytic fungi of Ipomoea pes-caprae reveals the superiority of in situ plant conservation over ex situ conservation from a mycological view by Yu-Hung Yeh, Roland Kirschner

“…In a culture-dependent approach, endophytic fungi were isolated under axenic conditions and identified to species, genus, or higher taxonomic ranks with DNA barcodes and morphology. In addition to yielding ca. 800 strains and over 100 morphospecies, a principal component analysis (PCA) of the distribution of the dominant fungal species showed clear differences in the composition of endophytic fungal species depending on the sampling sites. …”
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Species delimitation of newly collected spiders of the genus Pseudopoda (Araneae, Sparassidae) from Honghe Hani and Yi Autonomous Prefecture, Yunnan, China: An integrated morpholog... by Jianshuang Zhang, Tianqin Pan, He Zhang, Yuanqian Xing, Hao Yu, Yang Zhong

“…Detailed descriptions, diagnoses, photographs of the six species, and a distribution map for all 13 Pseudopoda species in Honghe Prefecture are provided. The DNA barcodes of the six species were obtained for species delimitation, sex matching, and future use.…”
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New banded Leporinus (Characiformes: Anostomidae) from the Madeira River basin, Brazil, and redescription of L. bleheri, based on integrative taxonomy by Mariana Pascoal Boaretto, Willian Massaharu Ohara, Lenice Souza-Shibatta, José Luís Olivan Birindelli

“…Both species specimens were cleared and double-stained for osteological description. DNA barcodes were used to investigate the genetic distance between samples and for species delimitation analyses. …”
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Deterministic genetic barcoding for multiplexed behavioral and single-cell transcriptomic studies by Jorge Blanco Mendana, Margaret Donovan, Lindsey Gengelbach O'Brien, Benjamin Auch, John Garbe, Daryl M Gohl

“…This method, called Targeted Genetically-Encoded Multiplexing (TaG-EM), involves inserting a DNA barcode just upstream of the polyadenylation site in a Gal4-inducible UAS-GFP construct so that the barcode sequence can be read out during single-cell sequencing, labeling a cell population of interest. …”
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Metatranscriptomics as a tool to identify fungal species and subspecies in mixed communities – a proof of concept under laboratory conditions by Vanesa R. Marcelino, Laszlo Irinyi, John-Sebastian Eden, Wieland Meyer, Edward C. Holmes, Tania C. Sorrell

“…Organisms in mixed microbial communities can now be sequenced and identified in a culture-independent way, usually using amplicon sequencing of a DNA barcode. Bulk RNA-seq (metatranscriptomics) has several advantages over DNA-based amplicon sequencing: it is less susceptible to amplification biases, it captures only living organisms, and it enables a larger set of genes to be used for taxonomic identification. …”
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Low-density SNP marker sets for genetic variation analysis and variety identification in cultivated citrus by Phuong Linh Nguyen, Jin-Kee Jung, Jee-Soo Park, Sung-Chur Sim

“…The resulting SNP sets are a resource to enhance the phenotype-based DUS testing by developing a DNA barcode system and thus facilitate new variety breeding and protection in citrus.…”
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