广谱人乳头瘤病毒DNA探针对尖锐湿疣和假性湿疣的原位杂交检测

“…对用肉眼形态、组织学特征和人乳头瘤病毒核壳抗原（ＨＰＶ－Ａｇ）来检测诊断为尖锐湿疣和假性湿疣的两组病例，用广谱人乳头瘤病毒ＤＮＡ探针，作核酸原位杂交检测，结果：尖锐湿疣组ＨＰＶ－ＤＮＡ检出率为３０／３３（９０．９％），而假性湿疣组检出率仅为１／３８（２．６％）（Ｐ＜０．０００１），提示假性湿疣并非为ＨＰＶ感染引起，或ＨＰＶ感染的可能性很小。…”
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Reduced Representation Libraries from DNA Pools Analysed with Next Generation Semiconductor Based-Sequencing to Identify SNPs in Extreme and Divergent Pigs for Back Fat Thickness by Samuele Bovo, Francesca Bertolini, Giuseppina Schiavo, Gianluca Mazzoni, Stefania Dall’Olio, Luca Fontanesi

“…Taking advantage from SNAPE for SNPs calling in sequenced DNA pools, 39,165 SNPs were identified; 1/4 of them were novel variants not reported in dbSNP. …”
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Non‐Invasive Tumor‐Naïve Minimal Residual Disease Detection of Liver Cancer by Incorporating Circulating Tumor DNA Features and Alpha‐Fetoprotein: A Prospective Study by Qingqi Ren, Shiyong Li, Guolin Zhong, Yunfei Li, Dao‐Ling Huang, Liangliang Zhang, Yumin Feng, Guanghui Long, Mao Mao

“…Identifying MRD through reliable prognostic biomarkers, such as circulating tumor DNA (ctDNA), could significantly benefit these patients by enabling timely intervention and improved outcomes. …”
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Genome-Wide Profiling of Human Papillomavirus DNA Integration into Human Genome and Its Influence on PD-L1 Expression in Chinese Uygur Cervical Cancer Women by Feng Yang-chun, Wang Sen-yu, Zhang Yuan, Huang Yan-chun

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O6-烷基鸟嘌呤-DNA 烷基转移酶基因多态性与肿瘤易感性的关系

“…【目的】通过比较表达在中国仓鼠卵巢(CHO)细胞中野生型和突变型O6-烷基鸟嘌呤-DNA烷基转移酶(AGT)对二氯乙基亚硝脲(BCNU)细胞毒性作用的反应差异,探测AGT错译突变体的功能意义。…”
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Melting curve analyses in the quantitative real-time polymerase chain reaction of methylated/non-methylated DNA toward the detection of oral cancer using gargle fluid by Yusaku Noma, Hayate Sawayama, Kouta Yamashiro, Keitaro Nishi, Haruka Yoshinaga, Ryota Takaoka, Tsuyoshi Sugiura, Tomonori Sasahira, Tomofumi Hamada, Yasuo Suda, Tatsuo Okui

“…The assay was performed by a qPCR of DNA from the gargle fluid of 20 patients with oral cancer and 20 healthy control subjects in the training set. …”
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Clinical Significance of Perioperative Minimal Residual Disease Detected by Circulating Tumor DNA in Patients With Lung Cancer With a Long Follow-up Data: An Exploratory Study by Shuta Ohara, MD, PhD, Kenichi Suda, MD, PhD, Sumedha Sudhaman, PhD, Akira Hamada, MD, PhD, Masato Chiba, MD, PhD, Masaki Shimoji, MD, PhD, Toshiki Takemoto, MD, PhD, Ekaterina Kalashnikova, PhD, Samantha K. Cheung, PhD, Michael Krainock, MD, PhD, Jordan Feeney, BS, Himanshu Sethi, MPH, Minetta C. Liu, MD, Junichi Soh, MD, PhD, Yasuhiro Tsutani, MD, PhD, Tetsuya Mitsudomi, MD, PhD

Subjects: “…Circulating tumor DNA (ctDNA)…”
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Utilização de DNA Tumoral Circulante na Análise de Prognóstico de Pacientes com Tumores Malignos Sólidos do Trato Gastrintestinal: Revisão Sistemática by Gabriel dos Santos Martins de Abreu, Mariana Albuquerque de Brito, Ana Paula de Souza Ramos

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Bottom Trawling and Multi‐Marker eDNA Metabarcoding Surveys Reveal Highly Diverse Vertebrate and Crustacean Communities: A Case Study in an Urbanized Subtropical Estuary by Jack Chi‐Ho Ip, Hai‐Xin Loke, Sam King Fung Yiu, Meihong Zhao, Yixuan Li, Yitao Lin, Chun‐Ming How, Jiezhang Mo, Meng Yan, Jinping Cheng, Vincent Chi‐Sing Lai, Leo Lai Chan, Kenneth Mei Yee Leung, Jian‐Wen Qiu

Subjects: “…eDNA biomonitoring…”
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Sequence and biochemical analysis of vaccinia virus A32 protein: Implications for in vitro stability and coiled-coil motif mediated regulation of the DNA-dependent ATPase activity by Uma Ramakrishnan, Tanvi Aggarwal, Kiran Kondabagil

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重组体pcDNA3.1(+)-tyr的构建及在小鼠NIH3T3细胞中的表达

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雷帕霉素和顺铂对Hep-2细胞DNA切除修复交叉互补基因1表达的协同作用

“…【目的】 探讨mTOR抑制剂雷帕霉素和顺铂对喉癌Hep-2细胞协同作用的机制【方法】 Hep-2细胞在雷帕霉素单药浓度为520 μmol/L;顺铂单药浓度为312 μmol/L;联合用药浓度为雷帕霉素5 μmol/L联合顺铂3 μmol/L中分别培养,检测Hep-2细胞AKT, mTOR, S6K和ERCC1(DNA切除修复交叉互补基因1)蛋白分别在3,6,12,24,48 h的表达情况【结果】 雷帕霉素单药干预Hep-2细胞12 h后,p-mTORS6K及ERCC-1表达表达下调,与对照组比较显著性,AKT蛋白表达在48 h增加,与对照组比较差异有统计学意义;顺铂单药干预Hep-2细胞时,ERCC-1表达12 h后增加,与对照组比较差异有统计学意义,p-mTORAKT和S6K蛋白表达差异无统计学意义;联合用药时,AKT蛋白表达在48 h增加,与对照组比较差异有统计学意义,p-mTORS6K在12 h后表达下降,与对照组比较差异有统计学意义,ERCC-1的表达与对照组比较没有差异有统计学意义【结论】 雷帕霉素和顺铂联合应用时,呈协同作用,这可能与雷帕霉素能诱导肿瘤细胞凋亡及影响ERCC-1的表达有关…”
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CHD6 has poly(ADP-ribose)- and DNA-binding domains and regulates PARP1/2-trapping inhibitor sensitivity via abasic site repair by Luc Provencher, Wilson Nartey, Peter M. Brownlee, Austin W. Atkins, Jean-Philippe Gagné, Lou Baudrier, Nicholas S. Y. Ting, Cortt G. Piett, Shujuan Fang, Dustin D. Pearson, Shaun Moore, Pierre Billon, Zachary D. Nagel, Guy G. Poirier, Gareth J. Williams, Aaron A. Goodarzi

“…CHD6 displays direct PAR-binding, interacts with PARP-1 and other PAR-associated proteins, and combined DNA- and PAR-binding loss eliminates CHD6 relocalization to DNA damage. …”
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Natural compound chaetocin induced DNA damage and apoptosis through reactive oxygen species‐dependent pathways in A549 lung cancer cells and in vitro evaluations by Qi Zhang, Feng Ruan, Maonan Yang, Qinghui Wen

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New tribe-level classification of Hypostominae (Loricariidae) based on optimization of morphological states on DNA-based relationships, with descriptions of three new tribes and two new genera by Jonathan W. Armbruster, Nathan K. Lujan

“…We re-optimized morphological character-state change by mapping states previously used to infer evolutionary history onto a composite phylogenetic tree inferred from DNA-sequence data. This revealed the strong influence on morphology-based phylogenies of a correlated suite of opercular character states related to the mechanism for cheek odontode eversion. …”
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Rationale and design of a multicentre randomised controlled trial on circulating tumour DNA-guided neoadjuvant treatment strategy for locally advanced rectal cancer (CINTS-R) by Bin Wu, Yi Xiao, Xiao Zhang, Tao Xu, Qian Liu, Wei Fu, Yang An, Yongheng Li, Guole Lin, Huadan Xue, Guoju Wu, Jiaolin Zhou, Hongwei Yao, Zhenjun Wang, Weijie Chen, Junyang Lu, Guannan Zhang, Xiaoyuan Qiu

“…Our previous study has shown that circulating tumour DNA (ctDNA) effectively reflects tumour burden and genetic characteristics and has significant predictive value for tumour recurrence, demonstrating great potential in guiding the choice of neoadjuvant strategies.Methods and analysis The CINTS-R trial is a multicentre, open-label, randomised controlled trial designed to evaluate the efficacy and safety of a ctDNA-guided neoadjuvant treatment strategy compared with conventional neoadjuvant therapy regime in patients with LARC. …”
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A Comprehensive Genetic Analysis of Mycotoxin-Producing Penicillium expansum Isolated from River Water Using Molecular Profiling, DNA Barcoding, and Secondary Structure Prediction by R. Ravikiran, G. Raghu and B. Praveen

Subjects: “…environmental contamination, dna barcoding, genetic characterization, molecular profiling, mycotoxin, pathogenic fungi, penicillium expansum, phylogenetic analysis, water quality monitoring…”
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Embryonic heat conditioning induces paternal heredity of immunological cross- tolerance: coordinative role of CpG DNA methylation and miR-200a regulation by Padma Malini Ravi, Tatiana Kisliouk, Shelly Druyan, Amit Haron, Mark A. Cline, Elizabeth R. Gilbert, Noam Meiri

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Plasma circulating cell–free DNA integrity and relative telomere length as diagnostic biomarkers for Parkinson’s disease and multiple system atrophy: a cross-sectional study by Chao Ying, Chao Han, Yuan Li, Mingkai Zhang, Shuying Xiao, Lifang Zhao, Hui Zhang, Qian Yu, Jing An, Wei Mao, Yanning Cai

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Rapid in vitro method to assemble and transfer DNA fragments into the JCVI-syn3B minimal synthetic bacterial genome through Cre/loxP system by Atsuko Uenoyama, Hana Kiyama, Mone Mimura, Makoto Miyata

“…Conventionally, Escherichia coli is used to construct DNA fragments for gene transfer into the syn3B genome through Cre/loxP system. …”
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