Modifying non-coding regions of linear DNA vaccines to explore the interplay of expression and inflammation in immunogenicity by David C. Stirling, Maria de Miguel Ferrer, Sungwon Kim, Madina Wane, Daniel Kysh, Lisa J. Caproni, John S. Tregoning

“…The interplay between antigen expression and the innate immune response impacts the overall immune response to DNA vaccines. Here, we explore the effect of altering non-coding 5’ regions, on the immunogenicity of a closed linear DNA platform, Doggybone™ DNA (dbDNATM), produced by a rapid and scalable cell-free method. …”
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Immunocytochemistry and DNA-image Cytometry in Diagnostic Effusion Cytology. II. Diagnostic Accuracy in Equivocal Smears by Helma Motherby, Nicolaus Friedrichs, Mary Kube, Bahram Nadjari, Kristiane Knops, Andreas Donner, Betty Baschiera, Peter Dalquen, Alfred Böcking

“…Furthermore, DNA‐image cytometry was performed. Data were correlated with patient follow‐up. …”
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Identification of species in the genus Nitraria L. (Nitrariaceae) based on nucleotide variability of nuclear ribosomal DNA by T. A. Poliakova, E. V. Banaev, M. A. Tomoshevich

“…Intragenomic polymorphism of ITS1 and ITS2 of nuclear ribosomal DNA sequences was analysed in 33 samples belonging to the Nitraria species N. schoberi, N. sibirica, and N. komarovii. …”
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DNA Damage Sensor γ-H2AX Is Increased in Preneoplastic Lesions of Hepatocellular Carcinoma by Yasunobu Matsuda, Toshifumi Wakai, Masayuki Kubota, Mami Osawa, Masaaki Takamura, Satoshi Yamagiwa, Yutaka Aoyagi, Ayumi Sanpei, Shun Fujimaki

“…Phosphorylated histone H2AX (γ-H2AX) is a potential regulator of DNA repair and is a useful tool for detecting DNA damage. …”
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Walking Among Pioneers – Sperm DNA Fragmentation and a Growing Focus on Male Factor Infertility by Robert Matthew Coward

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Nuclear DNA Content and Chromatin Pattern of Rat Rhabdomyosarcoma Cell Sublines with Different Metastatic Potentials by Jean Dufer, Marie-France Poupon, Sonia Yatouji

“…Data obtained were compared to karyotype, growth characteristics, and oncogene expressions of cell lines. The nuclear DNA content, the chromosome numbers, the cell sublines doubling times, and the distribution of cells within the cell cycle appear unrelated with this score. …”
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MITOCHONDRIAL DNA DIVERSITY IN THE GENE POOL OF THE NEOLITHIC AND EARLY BRONZE AGE CISBAIKALIAN HUMAN POPULATION by R. O. Trapezov, A. S. Pilipenko, V. I. Molodin

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A DNA-Methylated Sight on Autoimmune Inflammation Network across RA, pSS, and SLE by Xingqiang Wang, Dongyun Lei, Jie Ding, Shuang Liu, Li Tao, Fan Zhang, Jiangyun Peng, Jian Xu

“…In this study, we integrated 5 Cytokine-Chips from genome-wide DNA methylation datasets of the 3 kind of AIDs, delta-beta value was calculated for intergroup difference, and comprehensive bioinformatics analyses of cytokine genes with aberrant methylations were performed. 125 shared differential methylation variabilities (DMVs) were identified. …”
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Characterization of a Y-Family DNA Polymerase eta from the Eukaryotic Thermophile Alvinella pompejana by Sayo Kashiwagi, Isao Kuraoka, Yoshie Fujiwara, Kenichi Hitomi, Quen J. Cheng, Jill O. Fuss, David S. Shin, Chikahide Masutani, John A. Tainer, Fumio Hanaoka, Shigenori Iwai

“…Human DNA polymerase η (HsPolη) plays an important role in translesion synthesis (TLS), which allows for replication past DNA damage such as UV-induced cis-syn cyclobutane pyrimidine dimers (CPDs). …”
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A SuperLearner-based pipeline for the development of DNA methylation-derived predictors of phenotypic traits. by Dennis Khodasevich, Nina Holland, Lars van der Laan, Andres Cardenas

“…<h4>Background</h4>DNA methylation (DNAm) provides a window to characterize the impacts of environmental exposures and the biological aging process. …”
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DNA promoter task-oriented dictionary mining and prediction model based on natural language technology by Ruolei Zeng, Zihan Li, Jialu Li, Qingchuan Zhang

“…Abstract Promoters are essential DNA sequences that initiate transcription and regulate gene expression. …”
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How, What, and Where You Sample Environmental DNA Affects Diversity Estimates and Species Detection by Anish Kirtane, Leif Howard, Caitlin E. Beaver, Margaret E. Hunter, Gordon Luikart, Kristy Deiner

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Protocol for the quantitative detection of mtDNA in the supernatants of activated human naive CD8+ T cells by Linlin Li, Jun Jin

“…We then detail procedures for supernatant collection and quantification using the absolute copy number of mitochondrial DNA (mtDNA) released into the supernatants from in-vitro-activated naive CD8+ T cells. …”
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DNA-marker based identification of the RPV3 gene determining downy mildew resistance in grapevines by E. T. Ilnitskaya, M. V. Makarkina, S. V. Tokmakov, L. G. Naumova

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Cas12e orthologs evolve variable structural elements to facilitate dsDNA cleavage by Danyuan Li, Shouyue Zhang, Shuo Lin, Wenjing Xing, Yun Yang, Fengxia Zhu, Dingding Su, Chunlai Chen, Jun-Jie Gogo Liu

“…However, many differences exist in their in vitro dsDNA cleavage activities, reflecting the diversity in Cas12e’s enzymatic properties. …”
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Inactivation of the TIM complex components leads to a decrease in the level of DNA import into Arabidopsis mitochondria by T. A. Tarasenko, K. D. Elizova, V. I. Tarasenko, M. V. Koulintchenko, Yu. M. Konstantinov

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RIF1 integrates DNA repair and transcriptional requirements during the establishment of humoral immune responses by Eleni Kabrani, Ali Rahjouei, Maria Berruezo-Llacuna, Svenja Ebeling, Tannishtha Saha, Robert Altwasser, Veronica Delgado-Benito, Rushad Pavri, Michela Di Virgilio

“…RIF1 is a multifunctional protein that promotes antibody isotype diversification via its DNA end protection activity during class switch recombination. …”
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Oxidative stress and DNA lesions: the role of 8-oxoguanine lesions in Trypanosoma cruzi cell viability. by Pedro H N Aguiar, Carolina Furtado, Bruno M Repolês, Grazielle A Ribeiro, Isabela C Mendes, Eduardo F Peloso, Fernanda R Gadelha, Andrea M Macedo, Glória R Franco, Sérgio D J Pena, Santuza M R Teixeira, Leda Q Vieira, Alessandra A Guarneri, Luciana O Andrade, Carlos R Machado

“…The main consequence of oxidative stress is the formation of DNA lesions, which can result in genomic instability and lead to cell death. …”
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Spectroscopic Study of Cytosine Methylation Effect on Thermodynamics of DNA Duplex Containing CpG Motif by Václav Římal, Ondřej Socha, Josef Štěpánek, Helena Štěpánková

“…Effect of cytosine methylation on DNA duplexes was studied by using a model system of three self-complementary DNA octamers containing central CpG motif surrounded by a couple of AT base pairs, CAACGTTG, CATCGATG, and CTTCGAAG, and their analogues with the central cytosine methylated at C5 position. …”
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‘Gel-Stacks’ gently confine or reversibly immobilize arrays of single DNA molecules for manipulation and study by Susana Calle-Casteñeda, Eamon Winden, Alejandro Vasquez-Echeverri, Matthew Schickling, Evelyn Browning, Juan Pablo Hernandez Ortiz, David C Schwartz

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