Hydrogen‐Generating Magnesium Alloy Seed Strand Sensitizes Solid Tumors to Iodine‐125 Brachytherapy by Pan Hu, Letao Lin, Guanyu Chen, Dengyao Liu, Huanqing Guo, Meigui Xiao, Zhihui Zhong, Guang Yang, Bingchen Xu, Dongcun Huang, Sheng Peng, Yong Li, Yanling Zhang, Tao Huang, Fujun Zhang

“…In vitro experiments showed that hydrogen from AMASS degradation significantly inhibited tumor proliferation, increased apoptosis, disrupted redox homeostasis and mitochondrial membrane potential, reduced adenosine triphosphate levels, and induced DNA damage due to 125I radiation. In mouse xenograft and rabbit liver tumor models, hydrogen from AMASS showed superior therapeutic effects compared with 125I seeds alone, with no noticeable side effects. …”
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颈项透明层增厚胎儿的产前诊断及预后分析

“…【方法】 对１３５例妊娠早期（１１～１３＋６周）ＮＴ≥２．５ ｍｍ且行产前诊断的单胎病例进行回顾性分析，统计胎儿染色体核型、ＴＯＲＣＨ－ＤＮＡ／ＲＮＡ、地中海贫血基因检测结果，随访胎儿超声筛查结构异常情况及妊娠结局。按ＮＴ厚度将其依次分为５组，２．５～２．９、３．０～３．４、３．５～４．４、≥４．５ ｍｍ为第１～４组，颈部水囊瘤为第５组。…”
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Longitudinal profiles of oral microbiome in patients with nasopharyngeal carcinoma and their prognostic implications by Ting Zhou, Yan-Xia Wu, Xiao-Hui Zheng, Xi-Zhao Li, Wen-Qiong Xue, Tong-Min Wang, Yong-Qiao He, Shi-Hao Zhou, Yan Du, Jin-Ru Xie, Yi-Wei Chen, Li-Xia Lu, Ying Liao, Wei-Hua Jia

“…This study aims to address the dynamic changes in oral microbiome following cancer treatment and their prognostic implications in NPC patients.Patients and Methods Unstimulated whole saliva samples were collected from 23 NPC patients before and after treatment, with an average of 2.8 samples per patient, and post-treatment saliva samples were collected from additional 13 NPC patients that enrolled after treatment. Following DNA extraction and purification, the salivary microbiome was assessed by 16S rRNA gene amplicon sequencing targeting the V4 hypervariable region.Results Alpha-diversity of oral microbiome decreased progressively after treatment and during follow-up, and the beta-diversity of post-treatment samples differed significantly from the pre-treatment ones (R2 = 0.032, p < 0.001). …”
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Spotted fever group rickettsiae in black rats, pets, and humans in Zungarococha community, A rural area in the surroundings of Iquitos, Peru by Cusi Ferradas, Guillermo Salvatierra, David Payahuanca, Winnie Contreras, Andrés M. López-Pérez, Therangika A. Hangawatte, Diana León, Bruno M. Ghersi, Ricardo Gamboa, Katia Manzanares Villanueva, Viviana Pinedo-Cancino, Risa Pesapane, Gabriela Salmón-Mulanovich, Andrés G. Lescano, Janet Foley

“…Positive ectoparasites were further assessed by PCR and DNA amplicon sequencing. Non-parametric tests were used to evaluate factors associated with being seropositive among human adults. …”
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Whole genome sequencing of a novel carrageenan-degrading bacterium Photobacterium rosenbergii and oligosaccharide preparation by Jing Chen, Jing Chen, Runmin Chen, Kit-Leong Cheong, Zhuo Wang, Rui Li, Xuejing Jia, Qiaoli Zhao, Xiaofei Liu, Bingbing Song, Saiyi Zhong, Saiyi Zhong

“…In addition, there are no current reports of bacteria of the genus Photobacterium capable of secreting κ-carrageenanase or degrading carrageenan.MethodsIn the current study, strain GDSX-4 was obtained from Gracilaria coronopifolia after enrichment culture, primary screening and rescreening and was initially characterized by morphology and 16SrDNA. The pure culture of strain GDSX-4 was further subjected to bacterial genome sequencing assembly and bioinformatic analysis. …”
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STR profiling in a cohort of Saudi patients with acute leukemia by Husein A. Alhatim, PhD Student, Muhammad NH Abdullah, PhD, Suhaili A. Jamaludin, PhD, Armania B. Nurdin, PhD, Sayed A. Amer, PhD

“…Methods: Blood and saliva samples were collected from 27 patients diagnosed with acute leukemia in Riyadh City, KSA. DNA was extracted, and 15 STR loci were amplified. …”
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Interaction of human gut microbiota and local immune system in progression of colorectal adenoma (MIMICA-1): a protocol for a prospective, observational cohort study by Jurate Valciukiene, Egle Lastauskiene, Aida Laurinaviciene, Aida Laurinaviciene, Matas Jakubauskas, Marius Kryzauskas, Ruta Barbora Valkiuniene, Ruta Barbora Valkiuniene, Renaldas Augulis, Renaldas Augulis, Ausra Garnelyte, Justinas Kavoliunas, Ugne Silinskaite, Tomas Poskus

“…Biopsy specimens will be taken from the terminal ileum, right colon, left colon, and a pathological lesion in the colon (if present) to assess mucosa-associated microbiota composition and intestinal immunity response. DNA will be extracted from all samples and sequenced using the Illumina MiSeq platform. …”
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Effective gene therapy for metachromatic leukodystrophy achieved with minimal lentiviral genomic integrations by Lucas Tricoli, Sunetra Sase, Julia L. Hacker, Vi Pham, Maxwell Chappell, Laura Breda, Stephanie N. Hurwitz, Naoto Tanaka, Carlo Castruccio Castracani, Amaliris Guerra, Zhongqi Hou, Lars Schlotawa, Karthikeyan Radhakrishnan, Matthew Hogenauer, Aoife Roche, John Everett, Frederic Bushman, Peter Kurre, Rebecca Ahrens-Nicklas, Laura A. Adang, Adeline L. Vanderver, Stefano Rivella

“…To address this need, we increased the expression of ARSA cDNA at single integration by generating novel LVs, optimizing ARSA expression and enhancing safety. …”
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Production of all-triploid derived from gynogenetic homologous diploid crucian carp (♀) × diploid red crucian carp (♂) by Chongqing Wang, Yan Tang, Xiang Luo, Yuxin Zhang, Yue Zhou, Qingwen Xiao, Zhengkun Liu, Xinyi Deng, Xu Huang, Xidan Xu, Xiaowei Xu, Kun Zhang, Qinbo Qin, Shaojun Liu

“…The genetic characteristics of 5S rDNA in 3nRR individuals and their parental counterparts, as well as the measurable and countable traits, were explored. …”
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کلونینگ و توالی یابی ژن p- انسولین جدا شده از گیاه دارویی Momordica charantia و تحلیل بیوانفورماتیکی ساختار سه بعدی پروتئین... by مریم رحیمی, مجید عزیزی, احد یامچی, مجید شهبازی

“…نتایج RT-PCR با پرایمرهای اختصاصی ژن p-انسولین باند اختصاصی bp750 موید تکثیر صحیح cDNA بود. همچنین، بیان ژن p-انسولین در بافت آریل بذر رسیده بیشتر از سایر بافت های میوه بود. …”
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IFNβ drives ferroptosis through elevating TRIM22 and promotes the cytotoxicity of RSL3 by Huiyue Dong, Huiyue Dong, Ling Zhu, Ling Zhu, Jingjing Sun, Jingjing Sun, Qiuyan Chen, Qiuyan Chen, Pengyang Liu, Pengyang Liu, Wei Zhang, Huajing Zeng, Rong Lin, Rong Lin, Zongyang Yu, Zongyang Yu, Jun Lu, Jun Lu, Jun Lu, Jun Lu

“…BackgroundCyclic GMP-AMP synthase (cGAS)-stimulator-of-interferon genes (STING) pathway is a cytosolic DNA sensor system. The production of this pathway, interferon-β (IFNβ), could suppress the growth of tumor cells, yet it is unclear whether ferroptosis is involved in IFNβ-induced cell death.MethodsThe effects of IFNβ on ferroptosis were analyzed in HT1080, 4T1, HCT116 and 786-O cells. …”
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Assessing microbial biodiversity in oil palm seedlings using oil palm waste compost mixed media by Siti Suliza Salamat, Mohd Zulkhairi Mohd Yusoff, Mohd Ali Hassan Hassan, Mohd Huzairi Mohd Zainuddin, Yoshihito Shirai, Ahmad Husni Mohd Hanif, Mohd Shahkhirat Norizan, Fatini Mat Arisah, Mohammed Abdillah Ahmad Farid, Toshinari Maeda, Mohd Noor Mat Isa, Mohd Faizal Abu Bakar

“…Method: The study compared microbial biodiversity in soil media with compost amendments by employing DNA sequencing to assess microbial communities. …”
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Evaluating metagenomics and targeted approaches for diagnosis and surveillance of viruses by Sarah Buddle, Leysa Forrest, Naomi Akinsuyi, Luz Marina Martin Bernal, Tony Brooks, Cristina Venturini, Charles Miller, Julianne R. Brown, Nathaniel Storey, Laura Atkinson, Timothy Best, Sunando Roy, Sian Goldsworthy, Sergi Castellano, Peter Simmonds, Heli Harvala, Tanya Golubchik, Rachel Williams, Judith Breuer, Sofia Morfopoulou, Oscar Enrique Torres Montaguth

“…We tested samples consisting of a dilution series of a six-virus mock community in a human DNA/RNA background, designed to resemble clinical specimens with low microbial abundance and high host content. …”
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Sexual dimorphism in lung transcriptomic adaptations in fetal alcohol spectrum disorders by Vishal D. Naik, Dylan J. Millikin, Daniel Moussa, Hong Jiang, Alexander L. Carabulea, Joseph D. Janeski, Jiahui Ding, Kang Chen, Marta Rodriguez-Garcia, Sunil Jaiman, Stephen A. Krawetz, Gil Mor, Jayanth Ramadoss

“…Final libraries were checked for quality and quantity by Qubit hsDNA and LabChip. The samples were sequenced on the Illumina NovaSeq S4 Paired-end 150 bp. …”
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Microbial community dynamics in shallow-water grass-type lakes: Habitat succession of microbial ecological assembly and coexistence mechanisms by Chen Feng, Junping Lu, Tingxi Liu, Xiaohong Shi, Shengnan Zhao, Chunjian Lv, Yujiao Shi, Zixuan Zhang, Yuqi Jin, Jiaqi Pang, Aojie Sun

“…To explore the mechanisms of microbial ecological assembly and community succession in this unique habitat, 16 samples were collected from eight sites in Wuliangsuhai Lake. Second-generation DNA sequencing was applied to reveal the spatial dynamics of the bacterial community structure and distribution across two environmental media in this nutrient-rich shallow grassland lake and to elucidate the characteristics of the co-occurrence network. …”
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Pien Tze Huang Attenuates Neuro-inflammatory Injury in BV2 Microglial Cells Induced by Lipopolysaccharide via Inhibition of TLR4/MAPK Signaling Pathway by Zhenwei HUANG, Qing ZHANG, Lili HUANG, Xiaoqin ZHANG, Mingqing HUANG

“…Total RNA was extracted using TRIzol reagent and it was reverse-transcribed to synthesize the complementary DNA. And then the mRNA expression of IL-1β, IL-6 and TNF-α were determined using real time-qPCR. …”
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کاربرد نشانگرهای مورفولوژی و ISSR جهت شناسایی برخی ژنوتیپ‌های چای by شاهین جهانگیرزاده خیاوی, کوروش فلک‌رو, صنم صفایی چایی‌کار, سمر رمزی, احسان کهنه

“…پس از نمونه‌برداری از برگ‌های جوان و کاملا توسعه یافته،DNA ژنومی آنها استخراج شد و از 10 عدد نشانگرISSR برای بررسی روابط ژنتیکی 42 ژنوتیپ چای استفاده شد. …”
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Safety and efficacy of mesenchymal stromal cells mitochondria transplantation as a cell-free therapy for osteoarthritis by Ana Maria Vega-Letter, Cynthia García-Guerrero, Liliana Yantén-Fuentes, Carolina Pradenas, Yeimi Herrera-Luna, Eliana Lara-Barba, Felipe A. Bustamante-Barrientos, Masyelly Rojas, María Jesús Araya, Nicole Jeraldo, Constanza Aros, Francisca Troncoso, Daniela Poblete, Angela Court, Alexander Ortloff, Jose Barraza, Francesca Velarde, Carlos Farkas, Claudio Carril, Noymar Luque-Campos, Gonzalo Almarza, Maximiliano Barahona, Jose Matas, Lucas Cereceda, Rocío Lorca, Jorge Toledo, Karina Oyarce, Rolando Vernal, Andrés Caicedo, Andrea del Campo, Yessia Hidalgo, Roberto Elizondo-Vega, Farida Djouad, Maroun Khoury, Fernando E. Figueroa, Patricia Luz-Crawford

“…Moreover, the transcriptomic analysis showed the upregulation of genes involved in stress such as DNA reparative machinery and inflammatory antiviral responses. …”
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Multiomics analysis reveals the involvement of NET1 in tumour immune regulation and malignant progression by Jian Pang, Xiaoyan Huang, Ya Gao, Xinyu Guan, Lejia Xiong, Lun Li, Nana Yin, Mei Dai, Tong Han, Wenjun Yi

“…We also explored the relationships between NET1 and genes associated with epigenetic modifications, oncogenes, and tumour characteristics, such as RNA stemness scores (RNAss), DNA stemness scores (DNAss), the tumour mutation burden (TMB), and microsatellite instability (MSI). …”
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Methicillin-Resistant <i>Staphylococcus aureus</i>: The Shifting Landscape in the United Arab Emirates by Syrine Boucherabine, Rania Nassar, Lobna Mohamed, Maya Habous, Anju Nabi, Riyaz Amirali Husain, Mubarak Alfaresi, Seema Oommen, Hamda Hassan Khansaheb, Mouza Al Sharhan, Handan Celiloglu, Mubarak Hussain Raja, Eman Abdelkarim, Nishi Ali, Salman Tausif, Victory Olowoyeye, Nelson Cruz Soares, Mahmood Hachim, Danesh Moradigaravand, Dean Everett, Elke Mueller, Stefan Monecke, Ralf Ehricht, Abiola Senok

“…Methods: To investigate this influence, genomic profiling of 310 MRSA clinical isolates collected between February and November 2022 was performed using a DNA microarray-based assay. Results: Isolates were assigned to 22 clonal complexes and 72 distinct strain assignments. …”
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