Plasmodium falciparum transcription factor AP2-06B is mutated at high frequency in Southeast Asia but does not associate with drug resistance by Qiyang Shi, Changhong Wang, Wenluan Yang, Xiaoqin Ma, Jianxia Tang, Jiayao Zhang, Guoding Zhu, Yinlong Wang, Yaobao Liu, Xiaoqin He

“…One mutation, K3124R, was located in a PfAP2-06B AP2 domain.MethodsTo investigate transcriptional regulation by PfAP2-06B, ChIP-seq assays were performed on 3D7/PfAP2-06B-GFP schizonts using antibodies against GFP. The DNA sequences of the artemisinin-resistant CWX and the quinoline-resistant strains PfDd2 and Pf7G8 were analyzed for the genetic diversity of AP2-06B, compared with the Pf3D7 strain as a reference sequence. …”
Get full text

مکان‌یابی ارتباطی صفات پومولوژیک انگور (Vitis vinifera L.) با استفاده از نشانگرهای ISSR by میترا رازی, محمد اسماعیل امیری, رضا درویش زاده, حامد دولتی بانه, پدرو مارتینز گومز

“…صفات کیفی میوه در طی سه سال زراعی و در 10 تکرار اندازه‌گیری شدند. استخراج DNAی ژنومی بر اساس روش دویل و دویل (1987) انجام شد و از 17 نشانگر ISSR در واکنش PCR استفاده گردید. …”
Get full text

Analysis of stomatal characteristics of maize hybrids and their parental inbred lines during critical reproductive periods by Changyu Zhang, Changyu Zhang, Yu Jin, Yu Jin, Jinglu Wang, Jinglu Wang, Ying Zhang, Ying Zhang, Yanxin Zhao, Xianju Lu, Xianju Lu, Wei Song, Xinyu Guo, Xinyu Guo

Get full text

Screening and identification of two novel phosphate-solubilizing Pyrenochaetopsis tabarestanensis strains and their role in enhancing phosphorus uptake in rice by Xiaozhe Bao, Haifei Lu, Jinyao Zhao, Taotao Yang, Longmei Wu, Jixiang Zou, Qingchun Chen, Bin Zhang

“…An initial comparative sequence analysis of the hypervariable regions of the ITS rDNA and morphological analysis identified two strains belonging to the genus Pyrenochaetopsis, designated Pyrenochaetopsis tabarestanensis WFY-1 (PtWFY-1) and WFY-2 (PtWFY-2). …”
Get full text

Lentivirus‐mediated gene therapy for Fabry disease: 5‐year End‐of‐Study results from the Canadian FACTs trial by Aneal Khan, Dwayne L. Barber, William M. McKillop, C. Anthony Rupar, Christiane Auray‐Blais, Graeme Fraser, Daniel H. Fowler, Alexandra Berger, Ronan Foley, Armand Keating, Michael L. West, Jeffrey A. Medin

“…Our goal was to correct the enzyme deficiency in Fabry patients by transferring the cDNA for α‐gal A into their CD34+ hematopoietic stem/progenitor cells (HSPCs). …”
Get full text

Klebsiella pneumoniae induces dose-dependent shock, organ dysfunction, and coagulopathy in a nonhuman primate critical care model by Jeffrey R. Strich, Marcos J. Ramos-Benitez, Seth Warner, Heather Kendall, Sydney Stein, Andrew P. Platt, Sabrina C. Ramelli, Shelly J. Curran, Izabella Lach, Kiana Allen, Ashley Babyak, Luis J. Perez-Valencia, Mahnaz Minai, Junfeng Sun, Kevin M. Vannella, Derron Alves, Richard Herbert, Daniel S. Chertow

“…We observed dose-dependent bacteremia and circulating levels of Klebsiella pneumoniae DNA and endotoxin. Tachycardia and hypotension occurred in all animals and the study endpoint occurred in 8 of 12 animals that were euthanized. …”
Get full text

The First Large Identification of 3ANX and NX Producing Isolates of <i>Fusarium graminearum</i> in Manitoba, Western Canada by Maria Antonia Henriquez, Srinivas Sura, Sean Walkowiak, David Kaminski, Anne Kirk, Mark W. Sumarah, Parthasarathy Santhanam, Nina Kepeshchuk, Jules Carlson, E. RoTimi Ojo, Pam de Rocquigny, Holly Derksen

“…Using the same rice culture, genomic DNA was isolated, and the <i>Tri1</i> coding sequence along with its flanking regions (upstream and downstream of the <i>Tri1</i> gene) was amplified and sequenced. …”
Get full text

A non-randomised open-label exploratory ‘window of opportunity’ study of TG02 treatment in patients with locally advanced primary and recurrent RAS mutant colorectal cancer by Sara Roth, Kasmira Claire Wilson, Robert George Ramsay, Catherine Mitchell, Shienny Sampurno, Toan Duc Pham, Joseph Cherng Huei Kong, Stephen Q. Wong, Alexander Graham Heriot, Sanjeev Deva, Matthew Burge, Cecilie Sverdrup, Anne-Sophie Moller, Lukasz Kuryk, Jon Amund Eriksen, Magnus Jaderberg, John Raymond Zalcberg, Michael Michael

“…Tumour biopsies were analysed for tumour-infiltrating lymphocytes (TILs) and blood for CEA and ctDNA. TILs and tumouroids were cultured, characterised and tested for their killing efficacy. …”
Get full text

Clinicopathological and molecular characterization of astrocytoma by Xiaoyan Wu, Wenfeng Peng, Xu Zhang, Tao Tang, Ling Deng, Yuxia Xu, Xiaoyun Liu, Fang Wang, Wujian Peng, Jianrong Huang, Xiaoni Zhong

“…Fluorescence in situ hybridization (FISH) using break-apart probes showed EWRS1 breaks in three patients and MN1 breaks in one. Further DNA or RNA-targeted biallelic sequencing identified an EWSR1(Exon1-7)-BEND2(Exon2-14) fusion in case 1, and an EWSR1(Exon1-7)-BEND2(Intergenic) fusion in case 2. …”
Get full text

Polymorphisms in immunosuppression-related genes are associated with AML by Mingying Li, Mingying Li, Jingjing Ye, Jingjing Ye, Mengyuan Chang, Mengyuan Chang, Lei Feng, Lei Feng, Tingting Liu, Tingting Liu, Di Zhang, Di Zhang, Yuyan Wu, Yuyan Wu, Yuechan Ma, Yuechan Ma, Guangqiang Meng, Guangqiang Meng, Chunyan Ji, Chunyan Ji, Tao Sun, Tao Sun

“…Multi-omics analyses, such as single-cell transcriptomics, have revealed many potential new targets to improve AML prognosis from an immunological perspective.MethodsDNA from 307 AML patients and 316 healthy individuals were extracted. …”
Get full text

Long-term disease control in dedifferentiated liposarcoma: a case report on trabectedin priming followed by PD-1 inhibition by Johannes M. Waldschmidt, Johannes M. Waldschmidt, Lukas Haug, Lukas Haug, Christine Riedhammer, Christine Riedhammer, Christoph K. W. Deinzer, Marcus Zimmermann, Marcus Zimmermann, Anke Heidemeier, Anke Heidemeier, Peter Raab, Peter Raab, Maximilian Rudert, Maximilian Rudert, Anne Hendricks, Anne Hendricks, Johan F. Lock, Johan F. Lock, Viktoria Buck, Viktoria Buck, Andreas Rosenwald, Andreas Rosenwald, Hermann Einsele, Hermann Einsele, Peter Reichardt, Volker Kunzmann, Volker Kunzmann, Armin Wiegering, Armin Wiegering, Daniel Pink, Daniel Pink, K. Martin Kortüm, K. Martin Kortüm

“…Correlative molecular analyses revealed a sustained DNA damage repair machinery and downregulation of PD-1 protein expression in post-treatment tumor samples.ConclusionThis report provides exemplary insight on the feasibility and efficacy of sequential pre-treatment with trabectedin as a priming strategy for PD-1 inhibition in advanced DDLPS. …”
Get full text

Brucea javanica oil inhibited the proliferation, migration, and invasion of oral squamous carcinoma by regulated the MTFR2 pathway by Yihan Lai, Yihan Lai, Mingkang Li, Mingkang Li, Juan Zhan, Juan Zhan, Lin Jiang, Lin Jiang, Yuan Wu, Zhiyi Fang, Jianhan Zhou, Jianhan Zhou, Yujie Ma, Yisen Shao, Yisen Shao, Wei Wang, Wei Wang

“…MTFR2 knockdown (shRNA) and overexpression (cDNA) OSCC cells were constructed to evaluate the effect of MTFR2 on the proliferation and invasion of OSCC cells. …”
Get full text

LRRK2 Mutations and Asian Disease-Associated Variants in the First Parkinson’s Disease Cohort from Kazakhstan by Rauan Kaiyrzhanov, Akbota Aitkulova, Chingiz Shashkin, Nazira Zharkinbekova, Mie Rizig, Elena Zholdybayeva, Zharkyn Jarmukhanov, Vadim Akhmetzhanov, Gulnaz Kaishibayeva, Talgat Khaibullin, Altynay Karimova, Serik Akshulakov, Askhat Bralov, Nurlan Kissamedenov, Zhanar Seidinova, Anjela Taskinbayeva, Aliya Muratbaikyzy, Henry Houlden

“…Methods. 246 PD patients were consecutively recruited by movement disorder specialists from four medical centers in Kazakhstan, and clinicodemographic data and genomic DNA from blood were systematically obtained and shipped to the Institute of Neurology University College London together with DNAs from 200 healthy controls. …”
Get full text

Injectable Autocatalytic Hydrogel Triggers Pyroptosis to Stimulate Anticancer Immune Response for Preventing Postoperative Tumor Recurrence by Zhiping Rao, Yutong Zhu, Zhuang Chen, Yi Luo, Zuo Yang, Weijing Liu, Chaoqiang Qiao, Yuqiong Xia, Peng Yang, Dong‐Man Ye, Zhongliang Wang

“…Herein, an injectable hydrogel‐encapsulated autocatalytic copper peroxide (CP@Gel) therapeutic platform is designed and combine it with the clinical‐grade DNA methyltransferase inhibitor decitabine (DAC) to effectively inhibit TNBC growth and postoperative recurrence via pyroptosis, killing residual cancer cells that bypass apoptosis resistance while also improving immunogenicity and modulating immunosuppression to achieve an intense anti‐tumor immune response. …”
Get full text

Cloning, Characterization and Expression Regulation in Response to High-Temperature Stress of TRAF7 Gene in the Sea Cucumber Apostichopus japonicus by Yanfeng ZHAO, Meijie LIAO, Jianlong GE, Jinjin WANG, Yingeng WANG, Xiaojun RONG, Bin LI, Yanting TAN

“…In this study, based on the results of sea cucumber genome sequencing, the full-length sequence of sea cucumber TRAF7 (named AjTRAF7) cDNA was obtained using RACE technology. Its structural features and distribution in the genome were analyzed, and gene expression was examined in different tissues and under high-temperature stress. …”
Get full text

Unraveling the degradation mechanism of multiple pyrethroid insecticides by Pseudomonas aeruginosa and its environmental bioremediation potential by Hui Liu, Wen-Juan Chen, Zeling Xu, Shao-Fang Chen, Haoran Song, Yaohua Huang, Kalpana Bhatt, Sandhya Mishra, Mohamed A. Ghorab, Lian-Hui Zhang, Shaohua Chen

“…Strain PAO1 demonstrated effective soil bioremediation by removing etofenprox across different concentrations (25–100 mg·kg−1), with a degradation efficiency over 77 % within 15 days. Additionally, 16S rDNA high-throughput sequencing analysis revealed that introduction of strain PAO1 and etofenprox had a notable impact on the soil microbial community. …”
Get full text

Diversity and Complexity of CTXΦ and Pre-CTXΦ Families in <em>Vibrio cholerae</em> from Seventh Pandemic by Xiaorui Li, Yu Han, Wenxuan Zhao, Yue Xiao, Siyu Huang, Zhenpeng Li, Fenxia Fan, Weili Liang, Biao Kan

“…We identified two CTXΦ members and six pre-CTXΦ members with distinct <i>rstR</i> alleles, revealing complex chromosomal DNA integration patterns and arrangements of different prophages in these strains. …”
Get full text

بررسی تنوع ژنتیکی برخی ژنوتیپ‌های لیمو اسیدی (Citrus aurantifolia Swingle) ایران بر پایه نشانگر AFLP by شاهین جهانگیرزاده خیاوی, یوسف حمیداوغلی, بهروز گلعین, عاطفه صبوری

“…مواد و روش‌ها: پس از نمونه‌برداری از برگ‌های جوان و کاملا توسعه یافته، DNA ژنومی آنها استخراج شد. از نشانگر AFLP با کاربرد چهار ترکیب پرایمری حاصل از آغازگرهای EcoRI و MseI، شامل ECGC/MAGA، ECCA/MAGA، ECCA/MAGT و ECGC/MAAG برای بررسی روابط ژنتیکی 30 ژنوتیپ بومی از سه منطقه داراب (فارس)، منوجان (کرمان) و میناب (هرمزگان) و شش رقم وارداتی استفاده شد. …”
Get full text

ارزیابی تنوع ژنتیکی برخی ژنوتیپ‌های چای منسوب به دارجلینگ در ایران با استفاده از نشانگر‌های ISSRS by شاهین جهانگیرزاده خیاوی, رضا آزادی

“…پس از نمونه‌برداری از برگ‌های جوان و کاملا توسعه یافته،DNA ژنومی آنها استخراج شد و از 16 عدد نشانگرISSR برای بررسی روابط ژنتیکی 44 نمونه چای استفاده شد. …”
Get full text

Diel changes in the expression of a marker gene and candidate genes for intracellular amorphous CaCO3 biomineralization in Microcystis by Bruley, Apolline, Gaëtan, Juliette, Gugger, Muriel, Pancrace, Claire, Millet, Maxime, Gaschignard, Geoffroy, Dezi, Manuela, Humbert, Jean-François, Leloup, Julie, Skouri-Panet, Fériel, Callebaut, Isabelle, Benzerara, Karim, Duprat, Elodie

“…Moreover, several genes directly co-localized upstream and downstream of ccyA, on the same DNA strand show a similar expression pattern, including a cax gene encoding a calcium/proton exchanger and a gene encoding a protein with a domain also present in the N-terminal region of calcyanins in many iACC-forming cyanobacteria. …”
Get full text