Permafrost pore structure and its influence on microbial diversity: Insights from X-ray computed tomography by Nathan D. Blais, Joy M. O’Brien, Hannah Holland-Moritz, Lauren Farnsworth, Robyn A. Barbato, Thomas A. Douglas, Alexandra Contosta, Julie Bobyock, Erin C. Rooney, Taylor Sullivan, Jessica Gilman Ernakovich

“…The Euler characteristic number was used to measure pore connectivity and serve as a proxy for potential hydrological connectivity, as direct measurement of hydrological connectivity was not possible. DNA and RNA were extracted from the scanned permafrost and analyzed via amplicon sequencing of the 16S region to quantify the total and active microbial community diversity. …”
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Single-cell profiling reveals a reduced epithelial defense system, decreased immune responses and the immune regulatory roles of different fibroblast subpopulations in chronic atro... by Lin Lin, Tingxuan Huang, Lizhi Li, Yang Lin, Feng Chen, Ziyi Zheng, Jie Zhou, Yizhe Wang, Weihao You, Yujie Duan, Yawen An, Shiwei He, Weimin Ye

“…The mucous neck cells in CAG lesions showed upregulation of genes related to cell cycle transition, which may lead to aberrant DNA replication. Additionally, cells with the T exhaustion phenotype infiltrated under CAG condition. …”
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The anti-PD-L1/CTLA-4 bispecific antibody KN046 plus lenvatinib in advanced unresectable or metastatic hepatocellular carcinoma: a phase II trial by Da Xu, Hongwei Wang, Quan Bao, Kemin Jin, Ming Liu, Wei Liu, Xiaoluan Yan, Lijun Wang, Yanqiao Zhang, Guangyu Wang, Yue Ma, Zhigang Ma, Chunhui Zhang, Jiebing Tang, Sha Wang, Jiaohui Pang, Ting Xu, Kun Wang, Baocai Xing

“…The median overall survival (OS) was 16.4 (95% CI, 11.20-not estimable) months, and 12-month OS rate was 60.0% (95% CI, 45.87-71.55). Circulating tumor DNA status before the third cycle of treatment was associated with prognosis. …”
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Multi-omics elucidates the kidney damage caused by aquatic Cu via the gut-kidney axis in ducks by Mingcai Duan, Jinyu Liu, Zhaoxia Cai, Li Chen, Yong Tian, Wenwu Xu, Tao Zeng, Tiantian Gu, Lizhi Lu

“…Results indicate that Cu exposure causes pathological damage to the kidney, with a significant increase in the levels of TNFα, IL-6, and IL-1β in both serum and renal tissue. 16S rDNA analysis revealed that the relative abundances of Candidatus_Saccharimonas and Bacteroides were significantly reduced in the Cu-induced group. …”
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Anthocyanins from purple corn affect gut microbiota and metabolome in inflammatory bowel disease patients under infliximab infusion: the SiCURA pilot study by Mirco Vacca, Eduardo Maria Sommella, Marina Liso, Giulio Verna, Aurelia Scarano, Annamaria Sila, Margherita Curlo, Mauro Mastronardi, Katia Petroni, Chiara Tonelli, Bruno Di Jeso, Angelo Santino, Vincenzo Vestuto, Fabrizio Merciai, Gianluigi Giannelli, Pietro Campiglia, Maria De Angelis, Marcello Chieppa, Francesco Maria Calabrese

“…Patients in the intervention-arm followed a dietary supplementation with purple corn water-soluble extract, whereas control patients had a daily consumption of red fruit tea. 16S rDNA gene-sequencing and high-resolution mass-spectrometry metabo-lipidomics analyses were conducted on stool and sera samples, respectively. …”
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Radiomics prediction of MGMT promoter methylation in adult diffuse gliomas: a combination of structural MRI, DCE, and DTI by Yuying Liu, Yuying Liu, Zhengyang Zhu, Jianan Zhou, Han Wang, Huiquan Yang, Jinfeng Yin, Yitong Wang, Xin Li, Futao Chen, Qian Li, Zhuoru Jiang, Xi Wu, Danni Ge, Yi Zhang, Xin Zhang, Bing Zhang, Bing Zhang, Bing Zhang, Bing Zhang, Bing Zhang

“…PurposeTo assess the predictive value of radiomics features extracted from structural MRI, dynamic contrast enhanced (DCE), and diffusion tensor imaging (DTI) in detecting O6-methylguanine-DNA methyltransferase (MGMT) promoter methylation in patients with diffuse gliomas.MethodsRetrospective MRI data of 110 patients were enrolled in this study. …”
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Integrating BRCA testing into routine prostate cancer care: a multidisciplinary approach by SIUrO and other Italian Scientific Societies by Giulia Mammone, Simona Borghesi, Nicolò Borsellino, Anna Caliò, Roberta Ceccarelli, Alessia Cimadamore, Giario Natale Conti, Laura Cortesi, Rolando Maria D’Angelillo, Gaetano Facchini, Lorena Incorvaia, Alberto Lapini, Luigi Mearini, Giovanni Pappagallo, Paolo Prontera, Daniela Turchetti, Grazia Sirgiovanni, Sergio Bracarda, on behalf of the Italian Society of Uro-Oncology (SIUrO)

“…Abstract Prostate cancer (PCa) ranks among the most prevalent malignancies in men, with notable associations to Hereditary Breast and Ovarian Cancer Syndrome (HBOC) and Lynch Syndrome, both linked to germline likely pathogenetic variant/pathogenetic variant (LPV/PV) in genes involved in DNA repair. Among these genes, BRCA2 in PCa patients is the most frequently altered. …”
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Molecular characterization of ST15 carbapenem-resistant Klebsiella pneumoniae isolated in a single patient by Yongjin Hu, Rong Tang, Shanshan Jin, Jiahao Guan, Xiaoxiao Meng, Zengpeijie Dan, Ruilan Wang, Hong-Yu Ou, Jian Lu

“…Results: Our results revealed that 9 KP strains, isolated from the same patient, exhibited ‘resistance-sensitivity-resistance-sensitivity’ alternately to carbapenems. The differences in DNA fingerprint bands among the nine KP isolates were ≤3, which can be classified as the same PFGE type. …”
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Impact of donor CYP3A5 genotype on pharmacokinetics of tacrolimus in South African paediatric liver transplant patients by C Wheeler, C Masimirembwa, B Mthembu, J Botha, J Scholefield, J Fabian

“…Blood samples from 46 living liver donors were collected, their genomic DNA was extracted, and their CYP3A5 genotype was established (polymerase chain reaction and restriction fragment length polymorphism analysis, validated by Sanger sequencing). …”
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Divergent paths of mammary gland involution: unveiling the cellular dynamics in abruptly and gradually involuted mouse models by Sarmila Majumder, Sanjay Mishra, Neelam Shinde, Maria C. Cuitino, Morgan Bauer, Dinesh Ahirwar, Mustafa M. Basree, Vijaya Bharti, Kate Ormiston, Resham Mawalkar, Sara Alsammerai, Gautam Sarathy, Anna E. Vilgelm, Xiaoli Zhang, Ramesh K. Ganju, Bhuvaneswari Ramaswamy

“…Results AI results in rapid cell death, DNA repair response, and immunosuppressive myeloid cells infiltration, leading to a chronically inflamed microenvironment. …”
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Microwave-Assisted Synthesized ZnO@APTES Quantum Dots Exhibits Potent Antibacterial Efficacy Against Methicillin-Resistant Staphylococcus aureus Without Inducing Resistance by Du F, Niu J, Hong Y, Fang X, Geng Z, Liu J, Xu F, Liu T, Chen Q, Zhai J, Miao B, Liu S, Zhang Y, Chen Z

“…Once inside the bacteria, the ZnO@APTES QDs generate reactive oxygen species (ROS), causing DNA damage and bacterial cell death. Moreover, the ZnO@APTES QDs possessed good biocompatibility and demonstrated significant therapeutic efficacy against drug-resistant bacterial infections in both macrophage and mouse wound infection models.Conclusion: In summary, we have synthesized a highly effective water-soluble ZnO@APTES QDs that shows strong antibacterial and therapeutic efficacy against MRSA and other bacteria. …”
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Sox6 and ALDH1A1 Truncation by Asparagine Endopeptidase Defines Selective Neuronal Vulnerability in Parkinson's Disease by Shuke Nie, Bowei Li, Mengmeng Wang, Zijun Chen, Jiayan Ren, Zixuan Li, Xinli Xu, Zhengjiang Qian, Zhongyun Xie, Jianxin Han, Zhentao Zhang, Zhaohui Zhang, Yingjie Zhu, Zuxin Chen, Xifei Yang, Keqiang Ye

“…Here, it is shown that Legumain, also known as asparagine endopeptidase (AEP), is activated in a subgroup of SRY‐box transcription factor 6 /Aldehyde dehydrogenase 1 family member A1, (Sox6+/ALDH1A1+) neurons in the ventral tier of the SNpc and cleaves Sox6 and ALDH1A1, leading to repression of Special AT‐rich sequence binding protein 1 (Satb1) that is a dimeric/tetrameric transcription factor specifically binding to AT‐rich DNA sequences, and toxic dopamine metabolite accumulation. …”
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A c-type lectin HcLec1 with dual function of immunology and mineralization from the freshwater oyster (Hyriopsis cumingii Lea) by Xiaofeng Chen, Xiaoya Shen, Shijun Liu, Wenjuan Li, Wenjuan Li, He Wang, Jiale Li, Jiale Li, Zhiyi Bai, Zhiyi Bai

“…In vitro calcium carbonate crystallization assays were conducted to explore the direct role of HcLec1 in calcium carbonate crystal formation.ResultsThe HcLec1 gene sequence is a full-length cDNA of 1552 bp, encoding 240 amino acids. HcLec1 comprises an N-terminal signal peptide and a carbohydrate-recognition domain (CRD), with QPD (Gln-Pro-Asp) and MND (Met-Asn-Asp) motifs for polysaccharide binding. …”
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Structure and Expression of Two Sex-Related dmrt Genes in Oxyeleotris lineolata by Jiajia FAN, Dongmei MA, Huaping ZHU, Minghui LIN, Zaixuan ZHONG, Yuanyuan TIAN

“…In this study, the Oxldmrt1 and Oxldmrt3 cDNA sequence was obtained from gonad transcriptome of O. lineolata, identified using PCR. …”
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基于<italic>COI</italic>基因的浙江省内黄缘闭壳龟遗传多样性研究 by 陈敏, 秦媛, 林业宏, 任思齐, 叶嘉政, 郑善坚

“…为了解浙江省内不同黄缘闭壳龟（<italic>Cuora flavomarginata</italic>）群体的种质资源现状，基于线粒体DNA <italic>COI</italic>序列对来自浙江省内野外和养殖场的8个黄缘闭壳龟群体的遗传多样性和遗传结构进行比较分析。…”
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Shaoyao Gancao decoction alleviates paclitaxel-induced cognitive impairment by activating PTEN/PI3K/AKT pathway to inhibit NETs formation by Xu Ying, Su Yue, Hu Yuwen, Li Xiang, Zhou Ziyan, Yuan Ningning, Ji Xiaowei, Jiang Ruoyu, Wang Wenzhu, Zhang Yafeng, Zhai Guojie, Cheng Xiaolan

“…In addition, SGD could inhibit NETs formation, evidenced by decreasing the level of plasma MPO-DNA complex, as well as MPO and PAD4 expression. …”
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Vic9 mycobacteriophage: the first subcluster B2 phage isolated in Russia by Marina Zaychikova, Maja Malakhova, Dmitry Bespiatykh, Maria Kornienko, Ksenia Klimina, Aleksandra Strokach, Roman Gorodnichev, Arina German, Mikhail Fursov, Dmitry Bagrov, Dmitry Bagrov, Anna Vnukova, Alexandra Gracheva, Anastasia Kazyulina, Margarita Shleeva, Egor Shitikov

“…The genome of Vic9 comprises 67,543 bp of double-stranded DNA and encodes 89 open reading frames. Our analysis revealed unique features in Vic9, despite its close relationship to other B2 subcluster phages, highlighting its distinct characteristics even among closely related phages. …”
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3-methyl-4-nitrophenol disturbs the maternal-to-zygotic transition of early embryos by damaging mitochondrial function and histone modification by Fan Chen, An-Feng Luo, Kai-Xin Pan, Hao Gu, Chang-Fan Zhou, Wei Zeng, Song Liu, Adrian Molenaar, Hong-Yan Ren, Li-Jun Huo, Yan-Zhen Bi

“…Moreover, the study demonstrated that these gene expression changes were closely associated with mitochondrial dysfunction, evidenced by diminished mitochondrial membrane potential, reduced ATP production, aberrant expression of mitochondria-related genes, increased ROS accumulation, and heightened DNA damage in PNMC-treated embryos. Additionally, PNMC exposure induced defects in histone modification, as shown by altered levels of H3K9me3 and H3K27me3, H3K9ac and H3K27ac. …”
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Synthesis, Characterization, and Cytotoxic Activity of New Lanthanum(III) Complexes of Bis-Coumarins

“…Following a 24- hour treatment of BV-173 cells with lanthanum complex of <mml:math alttext="$chem{H_{2}}$"> <mml:msub> <mml:mtext>H</mml:mtext> <mml:mn>2</mml:mn> </mml:msub> </mml:math>L1 at 100 or 200 <mml:math alttext="$mu$"> <mml:mi>&#x03BC;</mml:mi> </mml:math>M led to a DNA-laddering. The findings suggest that the observed cytotoxicity of the lanthanum complex of <mml:math alttext="$chem{H_{2}}$"> <mml:msub> <mml:mtext>H</mml:mtext> <mml:mn>2</mml:mn> </mml:msub> </mml:math>L1 on BV-173 is at least partly mediated through induction of programmed cell death.…”
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NPRL2 gene therapy induces effective antitumor immunity in KRAS/STK11 mutant anti-PD1 resistant metastatic non-small cell lung cancer (NSCLC) in a humanized mouse model by Ismail M Meraz, Mourad Majidi, Renduo Song, Feng Meng, Lihui Gao, Qi Wang, Jing Wang, Elizabeth J Shpall, Jack A Roth

“…Restoration of NPRL2 induces DNA damage, apoptosis, and cell-cycle arrest. We investigated NPRL2 antitumor immune responses in aPD1R/KRAS/STK11mt NSCLC in humanized-mice. …”
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