Thioredoxin‐interacting protein (TXNIP) is a substrate of the NEDD4‐like E3 ubiquitin‐protein ligase WWP1 in cellular redox state regulation of acute myeloid leukemia cells by Sara Giovannini, Yanan Li, Rosalba Pecorari, Claudia Fierro, Claudia Fiorilli, Federica Corigliano, Valeria Moriconi, Ji Zhou, Anna De Antoni, Artem Smirnov, Sara Rinalducci, Anna Maria Timperio, Massimiliano Agostini, Jinping Zhang, Yufang Shi, Eleonora Candi, Gerry Melino, Francesca Bernassola

“…Increased ROS generation in WWP1‐depleted cells culminates in DNA strand breaks and subsequent apoptosis. Coherently with TXNIP stabilization following WWP1 inactivation, we also observed an impairment of both glucose up‐take and consumption. …”
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The multi-omics signatures of telomere length in childhood by Congrong Wang, Dries S. Martens, Mariona Bustamante, Rossella Alfano, Michelle Plusquin, Lea Maitre, John Wright, Rosemary R. C. McEachan, Johanna Lepeule, Remy Slama, Marina Vafeiadi, Leda Chatzi, Regina Grazuleviciene, Kristine B. Gutzkow, Hector Keun, Eva Borràs, Eduard Sabidó, Angel Carracedo, Georgia Escarami, Augusto Anguita-Ruiz, Dolors Pelegrí-Sisó, Juan R. Gonzalez, Martine Vrijheid, Tim S. Nawrot

“…Telomere length was quantified via qPCR in peripheral blood of the children. Blood DNA methylation, gene expression, miRNA expression, plasma proteins and serum and urinary metabolites were measured through microarrays or (semi-) targeted assays. …”
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Evidences of the Low Implication of Mosquitoes in the Transmission of Mycobacterium ulcerans, the Causative Agent of Buruli Ulcer by Rousseau Djouaka, Francis Zeukeng, Jude Daiga Bigoga, David N’golo Coulibaly, Genevieve Tchigossou, Romaric Akoton, Sylla Aboubacar, Sodjinin Jean-Eudes Tchebe, Clavella Nantcho Nguepdjo, Razack Adeoti, Innocent Djegbe, Manuele Tamo, Wilfred Fon Mbacham, Solange E. Kakou-Ngazoa, Anthony Ablordey

“…Methods. 7,218 adults and larvae mosquitoes were sampled from endemic and nonendemic villages and screened for MU DNA targets (IS2404, IS2606, and KR-B) using qPCR. …”
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Integrating microbial profiling and machine learning for inference of drowning sites: a forensic investigation in the Northwest River by Qin Su, Xiaofeng Zhang, Xiaohui Chen, Zhonghao Yu, Weibin Wu, Qingqing Xiang, Chengliang Yang, Jian Zhao, Ling Chen, Quyi Xu, Chao Liu

“…Our study investigates the application of microbiome analysis in inferring drowning sites, focusing on microbial diversity in water samples and lung tissues of drowned animals from different sites in the Northwest River. We utilized 16S rDNA sequencing to analyze microbial diversity in water samples and lung tissues, revealing distinct microbial signatures associated with drowning sites. …”
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Utilizing TP53 hotspot mutations as effective predictors of gemcitabine treatment outcome in non-small-cell lung cancer by Yen-Han Tseng, Trieu Thi My Tran, Jinghua Tsai Chang, Yu-Tang Huang, Anh Thuc Nguyen, Ian Yi‑Feng Chang, Yi-Tung Chen, Hao-Wen Hsieh, Yue-Li Juang, Peter Mu-Hsin Chang, Tzu-Yi Huang, Ying-Chih Chang, Yuh-Min Chen, Hsuan Liu, Chi-Ying F. Huang

“…This potential effectiveness might arise from its ability to disrupt DNA damage repair processes, leading to G2/M phase cell cycle arrest or an augmentation of mitotic abnormalities, eventually cause cell death. …”
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SRC enhanced cisplatin resistance in bladder cancer by reprogramming glycolysis and pentose phosphate pathway by Yuwen Gong, Dongyang Gao, Yibo Shi, Guangrui Fan, Xiaoquan Yu, Enguang Yang, Hui Cheng, Junqiang Tian, Hui Ding, Shanhui Liu, Shengjun Fu, Yan Tao, Yuan Shui, Liang Cheng, Lanlan Li, Zhiping Wang

“…SRC activates hexokinase2 which up-regulates glycolysis and especially the pentose phosphate pathway that leading to increased nucleotide synthesis and NADPH production which can neutralize reactive oxygen species (ROS) induced by cisplatin, thereby protecting bladder cancer cells from cisplatin-induced DNA damage. This phenomenon was effectively reversed by knockout of SRC and inhibition of SRC activity by the SRC inhibitor, eCF506. …”
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Comprehensive genomic and transcriptomic profiling of pulmonary nodules in synchronous multiple primary lung cancer by Xingsheng Liu, Kun Qian, Lei Su, Xiaoru Tian, Xin Zhao, Tengteng Wang, Li Han, Zhenzhen Li, Peilong Zhang, Ruotian Wang, Baodong Liu, Yuanbo Li, Xiaogang Tan, Yi Zhang

“…Methods We performed targeted DNA sequencing on tumor specimens and conducted bulk RNA transcriptome analysis on 53 multiple nodules originating from 26 lung cancer patients. …”
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Deciphering the role of epigenetics in plant pathogen resistance: Strategies for sustainable sugarcane management by Faisal Mehdi, Yuanli Wu, Shuzhen Zhang, Yimei Gan, Zhengying Cao, Shuting Jiang, Limei Zan, Benpeng Yang

“…Epigenetic mechanisms, such as histone modifications and DNA methylation, regulate these gene expressions related to biotic stress responses. …”
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Identification of ALEKSIN as a novel multi-IRF inhibitor of IRF- and STAT-mediated transcription in vascular inflammation and atherosclerosis by Aleksandra Antonczyk, Katarzyna Kluzek, Natalia Herbich, Mahdi Eskandarian Boroujeni, Bart Krist, Dorota Wronka, Anna Karlik, Lukasz Przybyl, Adam Plewinski, Joanna Wesoly, Hans A. R. Bluyssen

“…Using comparative in silico docking of multiple IRF–DNA-binding domain (DBD) models on a multi-million natural compound library, we identified the novel multi-IRF inhibitor, ALEKSIN. …”
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From structure prediction to function: defining the domain on the African swine fever virus CD2v protein required for binding to erythrocytes by Ana Luisa Reis, Anusyah Rathakrishnan, Vlad Petrovan, Muneeb Islam, Lynnette Goatley, Katy Moffat, Mai Tuyet Vuong, Yuan Lui, Simon J. Davis, Shinji Ikemizu, Linda K. Dixon

“…ABSTRACT African swine fever virus (ASFV) is a high-consequence pathogen posing a substantial threat to global food security. This large DNA virus encodes more than 150 open reading frames, many of which are uncharacterized. …”
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Lack of activity of HIV-1 integrase strand-transfer inhibitors on recombinase activating gene (RAG) activity at clinically relevant concentrations by Sally Demirdjian, Vincent N. Duong, Jennifer N. Byrum, Arabinda Nayak, Cooper B. McKinney, Jason K. Perry, Christian Callebaut, Karla K. Rodgers, Brie Falkard, Joy Y. Feng

“…We purified the first catalytically active recombinant human core RAG1–RAG2 complex and tested it in the presence of the co-factor human HMGB1 protein for the gel-based biochemical RAG DNA cleavage assay. Additionally, we optimized an extrachromosomal V(D)J recombination cellular assay using murine mCherry-core RAG1, full-length murine mCherry-RAG2, and a plasmid substrate green fluorescent protein (GFP) reporter system, transfecting them into cells in the absence or presence of inhibitors. …”
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Cloning and Expression Characteristics of BhARF4 Gene in Wax Gourd (Benincasa hispida) by Xiaoxin CHENG, Xuling ZHAI, Jinqiang YAN, Baochen WANG, Renliang MO, Wenrui LIU, Zhiming WU, Biao JIANG

“…【Objective】The ARF4 gene of wax gourd (Benincasa hispida) was cloned and its expression patterns in different tissues were studied by bioinformatics analysis, thereby providing a reference for further exploration of the function of BhARF4 in the development of wax gourd fruits.【Method】With the cDNA of the fruits of B227 on the day of flowering as template, specific cloning primers were designed according to the CDS sequence of BhARF4 in the reference gene. …”
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KMO-driven metabolic reconfiguration and its impact on immune cell infiltration in nasopharyngeal carcinoma: a new avenue for immunotherapy by Nijun Chen, Yuan Zong, Chen Yang, Lei Li, Yang Yi, Jiawen Zhao, Xiaoyu Zhao, Xianfei Xie, Xingmei Sun, Ning Li, Liting Jiang

“…Furthermore, KMO expression was negatively correlated with DNA repair mechanisms and hypoxia. In addition, KMO levels were inversely associated with the upregulation of both oxidative phosphorylation (OXPHOS) and glycolysis pathways within the NPC tumor microenvironment (TME). …”
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Association of HIVEP3 Gene and Lnc RNA with Femoral Neck Bone Mineral Content and Hip Geometry by Genome-Wide Association Analysis in Chinese People by Weiwei Hu, Jinwei He, Luyue Qi, Chun Wang, Hua Yue, Jiemei Gu, Hao Zhang, Yi Wang, Zhenlin Zhang

“…BMD and hip geometric parameters were measured by dual-energy X-ray absorptiometry. The genomic DNA of peripheral blood was extracted and analyzed by using Illumina Human Asian Screening Array-24 + v1.0 (ASA) gene chip. …”
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Exome sequencing reveals a rare damaging variant in GRIN2C in familial late-onset Alzheimer's disease by Elisa Rubino, Maria Italia, Elisa Giorgio, Silvia Boschi, Paola Dimartino, Tommaso Pippucci, Fausto Roveta, Clara Maria Cambria, Gabriella Elia, Andrea Marcinnò, Salvatore Gallone, Ekaterina Rogaeva, Flavia Antonucci, Alfredo Brusco, Fabrizio Gardoni, Innocenzo Rainero

“…Methods Affected and unaffected members from a Northern Italian family were included. Genomic DNA from family members was extracted and initially screened for pathogenic mutations in APP, PSEN1, and PSEN2, and screened for 77 genes associated with neurodegenerative conditions using NeuroX array assay. …”
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The CHK1 inhibitor prexasertib in BRCA wild-type platinum-resistant recurrent high-grade serous ovarian carcinoma: a phase 2 trial by Elena Giudice, Tzu-Ting Huang, Jayakumar R. Nair, Grant Zurcher, Ann McCoy, Darryl Nousome, Marc R. Radke, Elizabeth M. Swisher, Stanley Lipkowitz, Kristen Ibanez, Duncan Donohue, Tyler Malys, Min-Jung Lee, Bernadette Redd, Elliot Levy, Shraddha Rastogi, Nahoko Sato, Jane B. Trepel, Jung-Min Lee

“…Transcriptomic analysis revealed high levels of DNA replication-related genes (POLA1, POLE, GINS3) associated with lack of clinical benefit [defined post-hoc as PFS < 6 months]. …”
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Versatile nitrate-respiring heterotrophs are previously concealed contributors to sulfur cycle by Bo Shao, Yuan-Guo Xie, Long Zhang, Yang Ruan, Bin Liang, Ruochen Zhang, Xijun Xu, Wei Wang, Zhengda Lin, Xuanyuan Pei, Xueting Wang, Lei Zhao, Xu Zhou, Xiaohui Wu, Defeng Xing, Aijie Wang, Duu-Jong Lee, Nanqi Ren, Donald E. Canfield, Brian P. Hedlund, Zheng-Shuang Hua, Chuan Chen

“…Here, we applied microcosm incubations, community-isotope-corrected DNA stable-isotope probing, and metagenomics to characterize a cohort of heterotrophic denitrifiers in estuarine sediments that thrive by coupling sulfur oxidation with denitrification through chemolithoheterotrophic metabolism. …”
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Polymorphic variants of the genes for enzymes of the antioxidant system, apoptosis and inflammation as potential predictors of myocardial infarction by T. R. Nasibullin, V. V. Erdman, Y. R. Timasheva, I. A. Tuktarova, A. A. Petinseva, G. F. Korytina

“…The purpose of the study was to search for informative predictors of MI risk based on polygenic analysis of polymorphic variants of (1) the antioxidant defense enzyme genes PON1 (rs662), PON2 (rs7493), CAT (rs1001179), MSRA (rs10098474) and GSTP1 (rs1695); (2) the apoptosis genes CASP8 (rs3834129), TP53 (rs1042522) and BCL2 (rs12454712); and (3) the inflammation genes CRP (rs1205), CX3CR1 (rs3732378), IL6 (rs1800795) and CCL2 (rs1024611). 591 DNA samples were used in the study (280 patients with the onset at 30 to 60 years, with an average age of 46.02 ± 6.17, and 311 control subjects aged 30 to 62, with an average age of 44.65 ± 7.07). …”
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Conjunctival microbiota variations in a subset of middle-aged and elderly individuals from Beijing, China by Tian-Yao Zhao, Yu-Chen Wang, Ying-Juan Sun, Jun-Yue Wang, Xiao-Dan Jiang, Xue-Min Li, Ai-Hua Deng

“…Bacterial cultures were then conducted, followed by species identification through 16S rDNA gene sequencing. Clinical factors associated with positive or negative bacterial isolation rates were analyzed, including age, gender, meibomian gland dysfunction (MGD), history of hypertension, history of diabetes, history of cancer, history of infectious diseases and the habit of wearing masks. …”
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Blood-based epigenome-wide association study and prediction of alcohol consumption by Elena Bernabeu, Aleksandra D. Chybowska, Jacob K. Kresovich, Matthew Suderman, Daniel L. McCartney, Robert F. Hillary, Janie Corley, Maria Del C. Valdés-Hernández, Susana Muñoz Maniega, Mark E. Bastin, Joanna M. Wardlaw, Zongli Xu, Dale P. Sandler, Archie Campbell, Sarah E. Harris, Andrew M. McIntosh, Jack A. Taylor, Paul Yousefi, Simon R. Cox, Kathryn L. Evans, Matthew R. Robinson, Catalina A. Vallejos, Riccardo E. Marioni

“…Instead, molecular data such as blood-based DNA methylation (DNAm) could be used to derive a more objective measure of alcohol consumption by incorporating information from cytosine-phosphate-guanine (CpG) sites known to be linked to the trait. …”
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