The clinical utility of tissue polymerase chain reaction, tissue culture and tissue histology in blood culture-negative infective endocarditis in South Africa – insights from the G... by W Endres, P Mkoko, M Ntsekhe

“…Among patients undergoing surgery for IE, valve tissue may be evaluated for additional microbiological information by performing a broad-range 16S rDNA polymerase chain reaction (PCR) test, tissue culture and tissue histopathology. …”
Get full text

Rat Strain Differences in Susceptibility to Alcohol-Induced Chronic Liver Injury and Hepatic Insulin Resistance by Sarah M. DeNucci, Ming Tong, Lisa Longato, Margot Lawton, Mashiko Setshedi, Rolf I. Carlson, Jack R. Wands, Suzanne M. de la Monte

“…Among ethanol-fed rats, despite similar blood alcohol levels, LE rats had more pronounced steatohepatitis and fibrosis, higher levels of ALT, DNA damage, pro-inflammatory cytokines, ADH, ALDH, catalase, GFAP, desmin, and collagen expression, and reduced insulin receptor binding relative to FS rats. …”
Get full text

Morpho-cultural, pathogenic, and genetic characterization of Indian isolates of Macrophomina phaseolina causing charcoal rot in soybean by Laxman Singh Rajput, Sanjeev Kumar, Kriti Pathak, Palak Acharya, Divyanshu Goswami, Vennampally Nataraj, Maranna Shivakumar, Hemant Singh Maheshwari, Saloni Mandloi, Sapna Jaiswal, Asha Yadav, Raksha Vishwakarma

“…Phylogenetic analysis of the Internal Transcribed Spacer (ITS), a conserved rDNA region, revealed limited diversity among the 55 isolates. …”
Get full text

Transcriptional regulation of miR528-PPO module by miR156 targeted SPLs orchestrates chilling response in banana by Xiangjin Kong, Kuan Peng, Youxia Shan, Ze Yun, Tamas Dalmay, Xuewu Duan, Yueming Jiang, Hongxia Qu, Hong Zhu

“…Conversely, the browning was alleviated in STTM-miR156c silencing and OE-MaSPL4 samples. Furthermore, DNA affinity purification sequencing and MaSPL4-overexpressing transcriptome jointly revealed that MaSPL4 may mediate the transcription of genes related to lipid metabolism and antioxidation, in addition to the miR528-MaPPO module, demonstrating MaSPL4 as a master regulator in the fruit cold response network. …”
Get full text

Nucleic Acid Aptamers for Human Norovirus GII.4 and GII.17 Virus-Like Particles (VLPs) Exhibit Specific Binding and Inhibit VLPs from Entering Cells by Cheng C, Sun M, Li J, Xue Y, Cai X, Liu J, Wang X, Xu S, Xie Y, Zhang J

“…Here, we screened nucleic acid aptamers against the widespread HuNoV GII.4 and emerging HuNoV GII.17.Methods: After ten rounds of sieving for HuNoV GII.4 and GII.17 virus-like particles (VLPs), eight ssDNA aptamers were generated and characterized for each genotype.Results: Four of the eight aptamers generated for GII.4 VLP had dissociation constants (Kd) less than 100 nM, and all aptamers for GII.17 VLP had Kd less than 10 nM. …”
Get full text

Assessment of upper respiratory and gut bacterial microbiomes during COVID-19 infection in adults: potential aerodigestive transmission by Hafez Al-Momani, Andrew Nelson, Hadeel Al Balawi, Dua’a Al Balawi, Iman Aolymat, Ashraf I. Khasawneh, Hala Tabl, Ayman Alsheikh, AbdelRahman M. Zueter, Jeffrey Pearson, Christopher Ward

“…ELISA Pepsin tests and Reflux Symptom Index scores (RSI) were conducted for EOR assessment. DNA sequencing of the V4 region of the 16 S rRNA gene was performed for microbiome analysis. …”
Get full text

Mutations in 23S rRNA gene associated with clarithromycin resistance in Helicobacter pylori clinical isolates from Saint-Petersburg by Starkova D.A., Gladyshev N.S., Polev D.E., Egorova S.A., Svarval A.V.

“…The PCR products (1402 bp) of the 23S rRNA gene were sequenced by Sanger approach. The DNA sequences were compared to the H. pylori 26695 reference using Unipro UGENE v.38.1. …”
Get full text

Reproductive strategies in loggerhead sea turtle Caretta caretta: polyandry and polygyny in a Southwest Atlantic rookery by Laís Amorim, Lara Chieza, Jake A. Lasala, Sarah de Souza Alves Teodoro, Wesley D. Colombo, Ana Carolina Barcelos, Paula Rodrigues Lopes Guimarães, João Luiz Guedes da Fonseca, Ana Claudia Jorge Marcondes, Alexsandro Santos, Sarah Vargas

“…We analyzed 534 hatchlings from 43 nests associated with 42 females during the 2017/18 to 2019/20 nesting seasons, using four highly polymorphic, species-specific microsatellite markers (nDNA). Parentage tests were conducted to reconstruct paternal genotypes and determine the rates of multiple paternity within clutches. …”
Get full text

基于高通量测序分析骨肉瘤发生和转移的分子特征 by 许明贤, 李瑞希, 郭伟堂, 程鸣哲, 邹昌业

“…方法12例转移患者的原发灶与转移病灶肿瘤组织样本，使用第二代高通量测序（NGS）进行检测，其中9对panel检测（678个基因）以及3对全外显子检测（WES），分析比较骨肉瘤原发灶与转移灶的基因图谱差异：基因拷贝数变异通过EXCAVATOR检测；DNA层面的融合突变通过Lumpy检测；RNA层面的融合突变通过Defuse + STAR-Fusion，并通过Circos图显示突变的分布；Metascape 用于分析差异基因的 GO 和 KEGG 信号通路富集；使用Pyclone+Citup / LICHEE进行克隆进化分析。…”
Get full text

The application of Vavilov’s approaches to the phylogeny and evolution of cultivated species of the genus <i>Avena</i> L. by I. G. Loskutov, A. A. Gnutikov, E. V. Blinova, A. V. Rodionov

“…The methods and approaches that use DNA markers and genomic technologies, and are promising for the study of oat polymorphism and phylogeny have been actively researched recently. …”
Get full text

Characterization of the Occult Hepatitis B Virus Variants Circulating among the Blood Donors from Eastern India by Avik Biswas, Rajesh Panigrahi, Partha Kumar Chandra, Arup Banerjee, Sibnarayan Datta, Manisha Pal, Subhashish Chakraborty, Prasun Bhattacharya, Sekhar Chakrabarti, Runu Chakravarty

“…Among the study samples, 268 were anti-HBc positive/HBsAg negative, among which 65 (24.25%) were HBV DNA positive. Phylogenetic analysis revealed the presence of HBV/D (87.23%), HBV/A (8.51%), and HBV/C (4.26%) (P<0.0001). …”
Get full text

Late blight resistant potato hybrid clones in the VIR collection of plant genetic resources by E. V. Rogozina, N. A. Chalaya, M. A. Kuznetsova, V. N. Demidova, A. N. Rogozin, T. I. Smetanina, M. P. Beketova, O. A. Fadina, E. Е. Khavkin

“…Thirty-six genotypes were evaluated in the lab for resistance to highly aggressive P. infestans isolates from the collection of VNIIF (All-Russian Research Institute of Phytopathology). In parallel, DNA markers were used to reveal three genes that confer broad-spectrum resistance to P. infestance (Rpi).Results and conclusions. …”
Get full text

The adaptation of rainbow trout to warmer water: Oxidative damage in the germinal line by Vianel Sevastei, Sonia A. Crichigno, M. Victoria Santos, Andrea Trochine, Julio C. Painefilú, Noemí Zaritzky, Víctor E. Cussac

“…Our results suggest that the acquired thermal tolerance traits may be part of a wide spectrum of novel characteristics produced as a consequence of an enhanced mutation rate and/or a different DNA methylation pattern, induced by higher temperatures and mediated by ROS.…”
Get full text

FNDC5/irisin mitigates the cardiotoxic impacts of cancer chemotherapeutics by modulating ROS-dependent and -independent mechanisms by Manish Kumar, Abhishek Singh Sengar, Anushree Lye, Pranesh Kumar, Sukhes Mukherjee, Dinesh Kumar, Priyadip Das, Suvro Chatterjee, Adele Stewart, Biswanath Maity

“…Though the etiology of doxorubicin-dependent heart damage has yet to be fully elucidated, the ability of doxorubicin to damage DNA and trigger oxidative stress have been heavily implicated in the pathogenesis of chemotherapy-associated cardiomyopathy. …”
Get full text

Annotation and characterization of immunoglobulin loci and CDR3 polymorphism in water buffalo (Bubalus bubalis) by Yunlan Deng, Fengli Wu, Fengli Wu, Qianqian Li, Lidie Yao, Chengzhi Yang, Long Ma, Xinsheng Yao, Jun Li

“…The CDR3 repertoires were constructed using genomic DNA extracted from spleen samples of five healthy buffaloes. …”
Get full text

A multiplex method for rapidly identifying viral protease inhibitors by Seo Jung Hong, Samuel J Resnick, Sho Iketani, Ji Won Cha, Benjamin Alexander Albert, Christopher T Fazekas, Ching-Wen Chang, Hengrui Liu, Shlomi Dagan, Michael R Abagyan, Pavla Fajtová, Bruce Culbertson, Brooklyn Brace, Eswar R Reddem, Farhad Forouhar, J Fraser Glickman, James M Balkovec, Brent R Stockwell, Lawrence Shapiro, Anthony J O’Donoghue, Yosef Sabo, Joel S Freundlich, David D Ho, Alejandro Chavez

“…This multiplex approach is enabled by using cellular biosensors of viral protease activity combined with DNA-barcoding technology, as well as several design innovations that increase assay sensitivity and correct for plate-to-plate variation. …”
Get full text

Effect of aspirin dosage on oxidative stress and platelet reactivity in patients undergoing coronary artery bypass grafting (APRICOT): randomized controlled trial by Aleksandra Gąsecka, Rafał Kaczorowski, Katarzyna Pomykała, Tomasz Kucharski, Magdalena Gajewska, Dominika Siwik, Katarzyna Karoń, Maciej Małyszko, Jaromir Hunia, Jakub Michal Zimodro, Paweł Kowalczyk, Oliwia Zagrocka-Stendel, Małgorzata Dutkiewicz, Katarzyna Koziak, Ceren Eyileten, Marek Postuła, Mateusz Wondołkowski, Marcin Grabowski, Mariusz Kuśmierczyk, Radosław Wilimski

“…Platelet oxidative stress was measured as platelet mitochondria extracellular oxygen consumption rate and oxidatively damaged whole-blood DNA cleavage. Serum CD39 and CD73 levels were determined using ELISA. …”
Get full text

Molecular and immune landscape of tumours in geriatric patients with non-small cell lung cancer, melanoma and renal cell carcinoma by Abdul Rafeh Naqash, April K Salama, Emil Lou, Andrew Elliott, Wafik S El-Deiry, Chul Kim, Stephen V Liu, Anwaar Saeed, Hirva Mamdani, Khalil Choucair, Dipesh Uprety, Phillip Walker, Himisha Beltran, Matthew James Oberley, Azhar Saeed, Lujia Chen

“…Then, using next-generation sequencing and programmed death-ligand 1 (PD-L1) assessment, we evaluated gene expression differences between age groups and across tumour types, with a focus on ageing-related processes such as DNA damage response (DDR), immune checkpoint (IC) and metabolism-related genes. …”
Get full text

Bleomycin pollution and lung health: The therapeutic potential of peimine in bleomycin-induced pulmonary fibrosis by inhibiting glycolysis by Kangchen Li, Xuefang Liu, Ruilong Lu, Peng Zhao, Yange Tian, Jiansheng Li

“…Bleomycin, known for its DNA-damaging properties, is extensively used in oncology. …”
Get full text

A SH3b domain-containing peptidoglycan recognition protein from Magallana gigas with broad recognition spectrum by Yinan Li, Hongbo Lu, Chuanyan Yang, Zhuyun Liu, Weilin Wang, Chang Liu, Lingling Wang

“…In the present study, nine MgPGRP genes were identified in the updated genome of Magallana gigas, and the cDNA of MgPGRP5 which was consisted of a SH3b and a PGRP domain was cloned. …”
Get full text