Exploration of alcohol dehydrogenase EutG from Bacillus tropicus as an eco-friendly approach for the degradation of polycyclic aromatic compounds by Muhammad Naveed, Fatima Iqbal, Tariq Aziz, Ayesha Saleem, Tayyab Javed, Mahrukh Afzal, Muhammad Waseem, Metab Alharbi, Thamer H. Albekairi

“…In this study bacterial sample was collected from women’s vaginal discharge through streaking and spreading techniques. The DNA was extracted from bacterial culture and then the bacterium was identified through 16S rRNA which appeared to be B.tropicus. …”
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Selective Serotonin Reuptake Inhibitors: Antimicrobial Activity Against ESKAPEE Bacteria and Mechanisms of Action by Thiago Hideo Endo, Mariana Homem de Mello Santos, Sara Scandorieiro, Bruna Carolina Gonçalves, Eliana Carolina Vespero, Márcia Regina Eches Perugini, Wander Rogério Pavanelli, Gerson Nakazato, Renata Katsuko Takayama Kobayashi

“…Regarding their mechanisms of action as antibacterials, for the first time, increased membrane permeability was detected, as evidenced by heightened dye absorption, along with the increased presence of total proteins and dsDNA in the extracellular medium of <i>Escherichia coli</i> ATCC2 25922 and <i>Staphylococcus aureus</i> ATCC 25923, and oxidative stress was also detected in bacteria treated with sertraline and paroxetine, with reduced efficiency observed in the presence of antioxidants and higher levels of oxygen-reactive species evidenced by their reaction with 6-carboxy-2′,7′-dichlorodihydrofluorescein diacetate. …”
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Unraveling the pathogenesis of Barrett’s esophagus and esophageal adenocarcinoma: the “omics” era by Alberto Barchi, Giuseppe Dell’Anna, Giuseppe Dell’Anna, Luca Massimino, Luca Massimino, Francesco Vito Mandarino, Edoardo Vespa, Edi Viale, Sandro Passaretti, Vito Annese, Vito Annese, Alberto Malesci, Alberto Malesci, Silvio Danese, Silvio Danese, Federica Ungaro

“…Increasing interest has been gathered around small circulating genetic traces (circulating-free DNA and micro-RNAs) with a potential role as blood biomarkers. …”
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Pseudodominant Nanophthalmos in a Roma Family Caused by a Novel PRSS56 Variant by Lubica Dudakova, Pavlina Skalicka, Olga Ulmanová, Martin Hlozanek, Viktor Stranecky, Frantisek Malinka, Andrea L. Vincent, Petra Liskova

“…We have performed ocular examination and review of medical records in two individuals diagnosed with nanophthalmos (proband and her father) and one individual followed for bilateral congenital cataract and microcornea (uncle of the proband). DNA of subjects with nanophthalmos was analysed by exome sequencing. …”
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Identification and characterization of Serratia nematophila and Acinetobacter guillouiae from putrid-skin disease lesions in farmed Chinese spiny frog (Quasipaa spinosa) by Ling Guo, Xiyu Jin, Dan Yang, Li Wei, Jie Chen, Zhihua Lin, Li Ma

“…Two pathogenic strains, GL-XJ7 and GL-XJ11, were isolated from disease lesions and identified as Serratia nematophila and Acinetobacter guillouiae, respectively, through morphological, physiological, biochemical, and molecular analyses including 16S rDNA sequencing and phylogenetic tree construction. …”
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An integrated method for taxonomic identif ication of microorganisms by Yu. E. Uvarova, A. V. Bryanskaya, A. S. Rozanov, V. N. Shlyakhtun, E. A. Demidov, K. V. Starostin, T. N. Goryachkovskaya, S. V. Shekhovtsov, N. M. Slynko, S. E. Peltek

“…For accurate species-level identification of microorganisms, researchers today increasingly use a combination of standard microbiological cultivation and visual observation methods with molecular biological and genetic techniques that help distinguish between species and strains of microorganisms at the level of DNA or RNA molecules. The aim of this work was to identify microorganisms from the ICG SB RAS Collection using an integrated approach that involves a combination of various phenotypic and genotypic characteristics. …”
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A Case Study Identified a New Mutation in the TTN Gene for Inherited Hypertrophic Cardiomyopathy by Dong J, Liu M, Chen Q, Zha L

“…In the present study, WES and systematic genetic screening were performed to determine the genetic causes of HCM in Chinese HCM family.Materials and Methods: Peripheral blood genomic DNA was collected from 9 family members of a Chinese Han HCM pedigree, including an HCM proband. …”
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ارزیابی تنوع ژنتیکی گندم‌های دیپلوئید T. urartu بر اساس داده‌های ریخت شناختی و نشانگرهای RAPD by امیر عزیزیان, جواد مظفری, بهمن یزدی صمدی, علی اکبر شاه نجات بوشهری, محمد رضا نقوی

“…در ارزیابی‌های مولکولی DNA نیز 12 آغازگر RAPD چند‌شکلی بین مورفوتیپ‌ها نشان دادند، و 112 باند تولید شد که 75 باند (67%) در بین مورفوتیپ‌ها چندشکل بودند. …”
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Integration of ATAC-seq and RNA-seq reveals signal regulation during post-molt and inter-molt stages in muscle of Eriocheir sinensis by Bin Wang, Bin Wang, Jingjing Li, Mingzhi Zhang, Mingzhi Zhang, Anqi Li, Zhentao Song, Zhentao Song, Zhen He, Zhen He, Ruiqi Zhang, Ruiqi Zhang, Tong Hao, Tong Hao, Jinsheng Sun, Jinsheng Sun

“…GO enrichment analysis revealed that the up-regulated genes are largely associated with protein phosphorylation and phosphorus metabolism, while the down-regulated genes are mainly involved in DNA metabolism, transcription, cell adhesion, and G protein-coupled receptor (GPCR) signaling pathway. …”
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Long-term effects of combining anaerobic digestate with other organic waste products on soil microbial communities by Daniela Mora-Salguero, Denis Montenach, Manon Gilles, Vincent Jean-Baptiste, Sophie Sadet-Bourgeteau, Sophie Sadet-Bourgeteau

“…Soil microbial communities were characterized using high-throughput sequencing targeting 16S and 18S ribosomal RNA genes, following DNA extraction from soil samples collected in 2022, six years after the initial digestate application.ResultsThe results indicated that combining OWPs rich in stable and recalcitrant organic matter, such as BIO and FYM, with raw digestate, offers an improved fertilization practice. …”
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استفاده از روش توالی‌یابی اگزوم در شناسایی جهش‌های ژنتیکی کاردیومیوپاتی هایپرتروفیک و کاردیومیوپاتی اتساعی... by دینازهرا مختاری, فاطمه اکبریان, پریسا علی دوست سلیمی, سمیرا کلائی نیا

“…علاوه براین، داده‌‌های حاصل از توالی‌یابی اگزوم می‌تواند بینش گسترده‌تری را در مورد مکانیسم‌های مولکولی مؤثر در پاتوژنز کاردیومیوپاتی هایپرتروفیک و اتساعی ارایه دهد.روش‌ها: نمونه‌ی خون از دو فرد بیمار از دو خانواده ایرانی دارای کاردیومیوپاتی هایپرتروفیک و کاردیومیوپاتی اتساعی و اعضای خانواده‌های آن‌ها جمع‌آوری گردید، سپس با استفاده از روش Salting out، نمونه‌ی DNA آن‌ها استخراج شد. نمونه‌ها جهت انجام توالی‌یابی اگزوم به شرکت ماکروژن کره جنوبی ارسال شدند و سپس داده خام در مرکز قلب و عروق شهید رجائی مورد تجزیه و تحلیل قرار گرفت. …”
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Pharmacogenomics predictors of aromatic antiepileptic drugs-induced SCARs in the Iraqi patients by Ali Fadhel Ahmed, Dzul Azri Mohamed Noor, Majeed Arsheed Sabbah, Nur Fadhlina Musa, Nur Aizati Athirah Daud

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Thymus ad astra, or spaceflight-induced thymic involution by Wataru Muramatsu, Wataru Muramatsu, Maria Maryanovich, Maria Maryanovich, Maria Maryanovich, Taishin Akiyama, Taishin Akiyama, George S. Karagiannis, George S. Karagiannis, George S. Karagiannis, George S. Karagiannis, George S. Karagiannis, George S. Karagiannis

“…Cosmic radiation, an omnipresent hazard beyond Earth’s magnetosphere, accelerates DNA damage and cellular senescence in thymic epithelial cells, impairing thymopoiesis and increasing the risk of immune dysregulation. …”
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Insight into the quality deterioration induced by microorganisms in Litopenaeus vannamei during superchilling storage by Chuang Pan, Kaiying Yang, Xianqing Yang, Haiyun Wu, Chunsheng Li, Shuxian Hao, Yongqiang Zhao, Shengjun Chen, Xiaoshan Long

“…In this study, 6 microorganisms were identified as the biomarkers by 16S rDNA sequencing and 71 differential metabolites were identified by untargeted metabolomics. …”
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Tumor aggression-defense index–a novel indicator to predicts recurrence and survival in stage II-III colorectal cancer by Tong Wu, Lin Fang, Yuli Ruan, Mengde Shi, Dan Su, Yue Ma, Ming Ma, Bojun Wang, Yuanyu Liao, Shuling Han, Xiaolin Lu, Chunhui Zhang, Chao Liu, Yanqiao Zhang

“…Abstract Background Although the TNM staging system plays a critical role in guiding adjuvant chemotherapy for colorectal cancer (CRC), its precision for risk stratification in stage II and III CRC patients with proficient DNA mismatch repair (pMMR) remains limited. Therefore, precise predictive models and research on postoperative treatments are crucial for enhancing patient survival and improving quality of life. …”
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Differential Effects of Angelicin Analogues on NF-κB Activity and IL-8 Gene Expression in Cystic Fibrosis IB3-1 Cells by Ilaria Lampronti, Maria Giulia Manzione, Gianni Sacchetti, Davide Ferrari, Susanna Spisani, Valentino Bezzerri, Alessia Finotti, Monica Borgatti, Maria Cristina Dechecchi, Giorgia Miolo, Giovanni Marzaro, Giulio Cabrini, Roberto Gambari, Adriana Chilin

“…Analogues able to inhibit NF-κB/DNA interaction at lower concentration than TMA were found and selected to investigate their biological activity on IB3-1 cells induced with TNF-α. …”
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三个石骨症致病基因内的新突变及其基因型－表型

“…【方法】 收集３名石骨症患者及其父母的外周血，提取ＤＮＡ，ＰＣＲ扩增ＴＣＩＲＧ１及ＣＬＣＮ７基因的所有外显子及剪切位点序列，对ＰＣＲ产物进行Ｓａｎｇｅｒ测序并进行生物信息学分析，同时对新发现的突变在５０名正常对照中进行测序，以排除良性多态性。…”
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Precise Detection of Gene Mutations in Fine-Needle Aspiration Specimens of the Papillary Thyroid Microcarcinoma Using Next-Generation Sequencing by Feng-xia Yu, Min-xia Hu, Han-xue Zhao, Li-juan Niu, Xue-yu Rong, Wei-hua Li, Qiang Zhu, Jian-ming Ying, Ning Lyu

“…NGS was successfully performed in 114 specimens, while the remaining 21 samples were excluded due to insufficient amount/poor quality of DNA and sequencing failure. Of those 114 samples, 72 who were confirmed as having PTMC by postoperative histopathology were enrolled in our study, and the other 42 who had a follow-up with ultrasound were excluded. …”
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Increased cardiac macrophages in Sorbs2-deficient hearts: revealing a potential role for macrophage in responding to embryonic myocardial abnormalities by Beibei Hu, Xiangyang Liu, Xiangyang Liu, Xiangyang Liu, Shanshan Xiong, Qin Gong, Junjie Yang, Hongjun Shi, Hongjun Shi, Hongjun Shi, Min Zhang, Fei Liang, Zhen Zhang, Zhen Zhang

“…Partial depletion of macrophages led to downregulation of genes involved in lipid metabolism, muscle development and organ regeneration, alongside upregulation of genes associated with DNA damage-induced senescence and cardiomyopathy. …”
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Prevalence of β-lactam antibiotic resistance of Escherichia coli isolated from a neonatal intensive care unit by Jian Zhou, Jingqian Zhou, Min Chen, Pan Lü, Chunming Jiang

“…Conjugation experiments were conducted to analyze the horizontal transferability of resistance genes on plasmids. Genomic DNA was extracted for whole genome sequencing, construction of plasmid physical maps, locating resistance genes, and analyzing flanking regions and the resistance gene-related sequences. …”
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