Assembly and comparative analysis of the complete mitochondrial genome of red raspberry (Rubus idaeus L.) revealing repeat-mediated recombination and gene transfer by Huajie Zhang, Minghui Yan, Lijuan Li, Zhuo Jiang, Ye Xiong, Yusheng Wang, Caleb Onoja Akogwu, Olutayo Mary Tolulope, Hao Zhou, Yanxia Sun, Hengchang Wang

“…Key characteristics of the mitogenome were examined, including its gene composition, repetitive elements, and homologous DNA fragments. Additionally, we identified multiple recombination events in the mitogenome mediated by repetitive sequences The high-quality and well-annotated mitogenome for the known fruit red raspberry will provide essential genetic information for species classification, evolution analysis, and even genetic improvement in Rubus in the future.…”
Get full text

Hypercholesterolemia triggers innate immune imbalance and transforms brain infarcts after ischemic stroke by Ali Ata Tuz, Nils Hoerenbaum, Özgür Ulusoy, Adel Ahmadi, Alana Gerlach, Alexander Beer, Andreas Kraus, Anja Hasenberg, Nina Hagemann, Dirk M. Hermann, Matthias Gunzer, Matthias Gunzer, Vikramjeet Singh

“…The amount of plasma neutrophil extracellular traps (NETs) was measured using citH3-DNA complex ELISA. We found that HFD-induced cholesterolemia increased the number of circulating and splenic neutrophils in stroke mice but reduced bone marrow neutrophils compared to sham controls. …”
Get full text

Platelet-derived HMGB1 induces NETosis, exacerbating brain damage in the photothrombotic stroke model by Sang-A. Oh, Song-I. Seol, Dashdulam Davaanyam, Seung-Woo Kim, Ja-Kyeong Lee

“…Furthermore, serum levels of free DNA gradually and significantly increased, further supporting the induction of NETosis following PTS. …”
Get full text

RNA m6A involves in regulation of oxidative stress and apoptosis may via NF-kB pathway in cadmium-induced lung cells by Nan Zhang, Jie Yang, Yuan Zhao, Wenhong Li, Bo Zhao, Rongxian Li, Zuoshun He, Shiyan Gu

“…The subsequent experimental results indicated that levels of m6A and Fat mass and obesity associated protein (FTO) elevated, while Alkylated DNA repair protein alkB homolog 5 (ALKBH5) and YTH Domain Containing Protein 2 (YTHDC2) reduced with the increasing of cadmium treatment generations. …”
Get full text

Comprehensive Cervical Cancer Prevention in Tanzania (CONCEPT) study: Cohort profile by Ditte Søndergaard Linde, Vibeke Rasch, Julius D Mwaiselage, Rachel Manongi, Patricia Swai, Chunsen Wu, Bariki Mchome, Johnson Katanga, Crispin Kahesa, Susanne Kjaer

“…Biological samples included two cervical swabs for careHPV DNA testing, cytology, Hybrid Capture 2, genotyping and blood samples for HIV. …”
Get full text

Half-Elemental Diet Shifts the Human Intestinal Bacterial Compositions and Metabolites: A Pilot Study with Healthy Individuals by Jun Miyoshi, Daisuke Saito, Mio Nakamura, Miki Miura, Tatsuya Mitsui, Toru Kudo, Shinnosuke Murakami, Minoru Matsuura, Tadakazu Hisamatsu

“…Fecal samples were collected from all subjects at all time points, T1-3. Fecal DNA and metabolites were extracted from the samples. …”
Get full text

Microbial Metagenomes Across a Complete Phytoplankton Bloom Cycle: High-Resolution Sampling Every 4 Hours Over 22 Days by Brook L. Nunn, Emma Timmins-Schiffman, Miranda C. Mudge, Deanna L. Plubell, Gabriella Chebli, Julia Kubanek, Michael Riffle, William S. Noble, Elizabeth Harvey, Tasman A. Nunn, Tatiana Rynearson, Marcel Huntemann, Kurt LaButti, Brian Foster, Bryce Foster, Simon Roux, Krishnaveni Palaniappan, Supratim Mukherjee, T. B. K. Reddy, Chris Daum, Alex Copeland, I-Min A. Chen, Natalia N. Ivanova, Nikos C. Kyrpides, Tijana Glavina del Rio, Emiley A. Eloe-Fadrosh

“…Abstract In May and June of 2021, marine microbial samples were collected for DNA sequencing in East Sound, WA, USA every 4 hours for 22 days. …”
Get full text

Viral diversity in wild rodents in the regions of Canaã de Carajás and Curionopólis, State of Pará, Brazil by Adriana Freitas Moraes Monteiro, Adriana Freitas Moraes Monteiro, Fábio Silva da Silva, Ana Cecília Ribeiro Cruz, Ana Cecília Ribeiro Cruz, Sandro Patroca da Silva, Alice Louize Nunes Queiroz, Livia Medeiros Neves Casseb, Livia Medeiros Neves Casseb, Livia Carício Martins, Livia Carício Martins, Daniele Barbosa de Almeida Medeiros, Daniele Barbosa de Almeida Medeiros

“…These reads were categorized into 53 viral families, including both DNA and RNA viruses, with Retroviridae, Baculoviridae, and Microviridae being the most abundant. …”
Get full text

Evolutionary dynamics of mitochondrial genomes and intracellular transfers among diploid and allopolyploid cotton species by Jiali Kong, Jie Wang, Liyun Nie, Luke R. Tembrock, Changsong Zou, Shenglong Kan, Xiongfeng Ma, Jonathan F. Wendel, Zhiqiang Wu

“…Genome-wide mapping of nuclear organellar DNA transfers (NUOTs) in Gossypium revealed a nonrandom distribution of transfers in the nuclear genome. …”
Get full text

Dietary supplementation with Epimedium contributes to the improvement of hormone levels, gut microbiota, and serum metabolite composition in the Chinese forest musk deer (Moschus b... by Shan Xie, Shan Xie, Qinlin Yang, Zaixiang Ying, Zaixiang Ying, Mingcheng Cai, Wenqiao Fan, Hanyu Gao, Hanyu Gao, Xiaolan Feng, Yongjiang Wu

“…The results indicated that the inclusion of Epimedium in the diet increased dry matter intake (DMI) and improved the ratio of feed to gain (F/G), with an increase in fecal testosterone levels (p < 0.05). 16S rDNA sequencing analysis revealed that Epimedium enhanced the richness and diversity of the gut microbiota in the Chinese forest musk deer, increasing the relative abundance of beneficial bacteria such as Firmicutes, while reducing the relative abundance of the potentially pathogenic Proteobacteria (p < 0.05). …”
Get full text

Fatty Acid Content and Profile in <i>Ulva lactuca</i> in Response to Exposure to Variable Growth Conditions in Indoor Photobioreactors by Nabeel Gnayem, Razan Unis, Rima Gnaim, Alexander Chemodanov, Álvaro Israel, Jallal Gnaim, Alexander Golberg

“…The taxonomic identity of <i>U. lactuca</i> was confirmed through DNA sequencing using 3 markers (rbcL, ITS, and tufa). …”
Get full text

Influence of Rapid Urbanization on Thyroid Autoimmune Disease in China by Yingchao Chen, Bing Han, Jie Yu, Yi Chen, Jing Cheng, Chunfang Zhu, Fangzhen Xia, Ningjian Wang, Yingli Lu

“…Serum TPOAb, TGAb, and thyrotropin (TSH) were measured, and ultrasonography of the thyroid was performed in all subjects. DNA was extracted from all subjects, and four SNPs were selected for genotyping. …”
Get full text

Association between Polymorphisms in Interleukin-17A and -17F Genes and Chronic Periodontal Disease by Jôice Dias Corrêa, Mila Fernandes Moreira Madeira, Renata Gonçalves Resende, Jeane de Fátima Correia-Silva, Ricardo Santiago Gomez, Danielle da Glória de Souza, Mauro Martins Teixeira, Celso Martins Queiroz-Junior, Tarcília Aparecida da Silva

“…Material and Methods. Genomic DNA was obtained from 30 CP patients and 30 healthy controls (HCs). …”
Get full text

Gut microbiota and kidney function in autosomal dominant polycystic kidney disease participants in Cameroon: a cross-sectional study by Inès Obolo Nwaga, Victorine Bandolo Nzana, Rhoda Nsen Bughe, Isaac Dah, Cheboh Cho-Fon, Maimouna Mahamat, Emmanuelle Ndjong, Aristide Nono, Jean Claude Mballa, Fon Abongwa Acho, Vicky Ama Moor, Wilfred Fon Mbacham, François Folefack Kaze

“…The estimated glomerular filtration rate (eGFR) was calculated from the serum creatinine concentration using the 2009 Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI) equation. Microbial DNA extracted from stool specimens and amplified by qPCR was used to quantify Enterobacteriaceae, Bifidobacterium and Lactobacillus abundance. …”
Get full text

广东省2009-2012年呼吸道合胞病毒的流行病学特征

“…【方法】 2009年7月至2012年6月期间在广东省25家哨点医院采集急性呼吸道感染病例咽拭子标本,提取标本RNA,反转录成cDNA,利用巢式PCR进行RSV检测;对RSV阳性标本进行其他6种呼吸道病毒的检测,了解混合感染情况?…”
Get full text

Incidence and estimated risk of residual transmission of hepatitis a virus and parvovirus B19 by blood transfusion in the state of Rio De Janeiro – Brazil: a retrospective study by Arthur Daniel Rocha Alves, Mariana Magaldi, Ana Carolina Sathler de Menezes, Josiane Iole França Lopes, Carlos Alexandre da Costa Silva, Jaqueline Mendes de Oliveira, Camila Mattos, Leonardo Bastos, Luiz de Melo Amorim Filho, Marcelo Alves Pinto, Luciane Almeida Amado

“…Results HAV-RNA and B19V-DNA were detected in 1.66 (95% CI 0.26–5.48) and 3.32 (95% CI 1.00-7.81) per 1000 donors, respectively. …”
Get full text

Efficacy and safety of interferon-alpha 1b injection into the intervertebral foramen with ultrasonic guidance in patients with postherpetic neuralgia: study protocol for a randomiz... by Hui-Min Hu, Hui-Min Hu, Wen-Hui Liu, Wen-Hui Liu, Chen Li, Chen Li, Qing Shi, Qing Shi, Chun-Hua Liu, Chun-Hua Liu, An-Xiang Liu, An-Xiang Liu, Yi-Fan Li, Yi Zhang, Peng Mao, Bi-Fa Fan

“…The secondary outcomes will include the 36-item short-form, dosage and duration of painkillers taken, viral load of varicella-zoster virus DNA, humoral cytokine level, and dosage and frequency of rescue medication. …”
Get full text

48-Week Outcome after Cessation of Nucleos(t)ide Analogue Treatment in Chronic Hepatitis B Patient and the Associated Factors with Relapse by Wen-xiong Xu, Qian Zhang, Xiang Zhu, Chao-shuang Lin, You-ming Chen, Hong Deng, Yong-yu Mei, Zhi-xin Zhao, Dong-ying Xie, Zhi-liang Gao, Chan Xie, Liang Peng

“…Age (OR = 1.06, 95% CI = 1.02–1.10; p=0.003), the HBsAg level (OR = 2.21, 95% CI = 1.47–3.32; p<0.001), and positive origin HBeAg status (OR = 0.32, 95% CI = 0.14–0.74; p=0.008) were predictive factors to virologic relapse. HBV DNA level (OR = 1.34, 95% CI = 1.13–1.58; p<0.001) was predictive factor to retreatment. …”
Get full text

Structural engineering of stabilized, expanded epitope nanoparticle vaccines for HPV by Michaela Helble, Michaela Helble, Xizhou Zhu, Pratik S. Bhojnagarwala, Kevin Liaw, Yangcheng Gao, Amber Kim, Kelly Bayruns, Madison E. McCanna, Joyce Park, Kylie M. Konrath, Kylie M. Konrath, Sam Garfinkle, Sam Garfinkle, Taylor Brysgel, David B. Weiner, David B. Weiner, Daniel W. Kulp, Daniel W. Kulp

“…One of the primary goals of cancer vaccination is to increase the potency and diversity of anti-tumor T-cell responses; one strategy to do so involves the delivery of full-length cancer antigens scaffolded onto DNA-launched nanoparticles to improve T-cell priming. …”
Get full text

Nas tramas do neoliberalismo: o impacto do Airbnb no setor hoteleiro e no mercado imobiliário em São Paulo e no Rio de Janeiro, Brasil by Rita de Cássia Ariza da Cruz, Cristina Pereira de Araujo, Luciano Muniz Abreu

“…Os dados sobre o Airbnb foram obtidos da plataforma AirDna, incluindo os anos completos de 2021, 2022, 2023 e janeiro de 2024, e em relação ao mercado imobiliário nas duas cidades, a principal fonte de dados foi a SECOVI, uma entidade privada que representa a categoria econômica de empresas de compra, venda, aluguel e administração de imóveis. …”
Get full text