No association of ABO blood groups and Rh factor with primary liver cancer in cirrhotic patients: a single-center cross-sectional study by Liyan Dong, Liyan Dong, Yuhang Yin, Yuhang Yin, Huiyuan Lu, Huiyuan Lu, Di Sun, Di Sun, Dongyang Wang, Deli Zou, Xingshun Qi

“…Adjusted odds ratios (aORs) with 95% confidence intervals (CIs) were calculated after adjusting for gender, age, family history of liver cirrhosis, HBV-DNA positivity, and etiology of cirrhosis. Subgroup analyses were performed according to the etiology of liver cirrhosis.ResultsOverall, 1,158 cirrhotic patients without PLC and 240 cirrhotic patients with PLC were included in the study. …”
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Whole Exome Sequencing Uncovers Germline Variants of Cancer-Related Genes in Sporadic Pheochromocytoma by Milena Urbini, Margherita Nannini, Annalisa Astolfi, Valentina Indio, Valentina Vicennati, Matilde De Luca, Giuseppe Tarantino, Federica Corso, Maristella Saponara, Lidia Gatto, Donatella Santini, Guido Di Dalmazi, Uberto Pagotto, Renato Pasquali, Andrea Pession, Guido Biasco, Maria A. Pantaleo

“…Whole exome sequencing of tumor DNA was performed on a set of twelve PCCs clinically defined as sporadic. …”
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Associations of Serum Cystatin C, DNAm Cystatin C, Renal Function, and Mortality in U.S. Adults by Yu-Wei Fang, Wei-Chung Huang, Chikang Wang, Chien-Yu Lin

“…Serum cystatin C is a well-established marker of renal function and a valuable predictor of health risks and mortality. DNA methylation-predicted cystatin C (DNAmCystatinC), an advanced epigenetic biomarker, serves as a proxy for serum cystatin C levels. …”
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Clinical significance and pro-oncogenic function of DBF4 in clear cell renal cell carcinoma by Liuyan Chen, Lvying Wu, Minying Tang, Yuanhang Cheng, Kuanyin Wang, Jianan Zhang, Wenyi Deng, Lingfeng Zhu, Jin Chen

“…DBF4 plays a critical role in DNA replication and participates in various biological functions, making it an attractive target for cancer treatment. …”
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The co-occurrence patterns and assembly mechanisms of microeukaryotic communities in geothermal ecosystems of the Qinghai-Tibet Plateau by Bingjie Yan, Bingjie Yan, Xiaodong Li, Xiaodong Li, Nanqian Qiao, Nanqian Qiao, Zhen Da, Zhen Da, Jiajie Xu, Jiajie Xu, Chuanqi Jiang, Sang Ba, Sang Ba

“…In this study, we used high-throughput 18S rDNA sequencing in combination with environmental factor analysis to investigate the co-occurrence patterns, assembly mechanisms, and responses to environmental changes of microeukaryotic communities in sediment and water samples from 36 geothermal springs across different temperature gradients in southern Tibet. …”
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Los olvidados: Non-BRCA variants associated with Hereditary breast cancer in Mexican population by Dione Aguilar, María Lourdes Garza-Rodríguez, Carolina Elizabeth Muñiz-Garza, Fernando Alcorta Nuñez, Cynthia Mayte Villarreal-Garza, Oscar Vidal-Gutiérrez, Diana Cristina Pérez-Ibave, Carlos Horacio Burciaga-Flores

“…Among the group of non-BRCA genes, six are involved in the homologous repair pathway (HR), and three are related to DNA damage repair (DDR) pathways. In analyzing GPVs in molecular pathways, both have similar frequencies with no statistical difference for BC. …”
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Biglycan-driven risk stratification in ZFTA-RELA fusion supratentorial ependymomas through transcriptome profiling by Konstantin Okonechnikov, David R. Ghasemi, Daniel Schrimpf, Svenja Tonn, Martin Mynarek, Jan Koster, Till Milde, Tuyu Zheng, Philipp Sievers, Felix Sahm, David T.W. Jones, Andreas von Deimling, Stefan M. Pfister, Marcel Kool, Kristian W. Pajtler, Andrey Korshunov

“…Here, we performed methylation-based DNA profiling and transcriptome RNA sequencing analysis of 80 ST-EPN ZFTA-RELA investigating the clinical significance of various molecular patterns. …”
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Long-term results of antiviral therapy in patients with chronic hepatitis C with sustained virologic response by T. M. Ignatova, T. N. Lopatkina, V. P. Chulanov, Ye. V. Gaydasheva, S. N. Batskikh, G. V. Mikhaylovskaya, D. T. Abdurakhmanov, S. Yu. Karpov, Ye. N. Nikulkina, O. A. Chernova

“…In 114 patients RNA HCV and DNA HBV in blood serum and peripheral mononuclear blood cells were studied by polymerase chain reaction (PCR) with fluorescent hybridization detection in «real time» mode (sensitivity of 10 IU/ml for HCV and 5 IU/ml for HBV).Results. …”
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Loss of Lkb1 cooperates with BrafV600E and ultraviolet radiation, increasing melanoma multiplicity and neural‐like dedifferentiation by Kimberley McGrail, Elena González‐Sánchez, Paula Granado‐Martínez, Roberto Orsenigo, Yuxin Ding, Berta Ferrer, Javier Hernández‐Losa, Iván Ortega, Juan Martín‐Caballero, Eva Muñoz‐Couselo, Vicente García‐Patos, Juan A. Recio

“…In concordance with these findings and previous reports, Lkb1‐null irradiated mice exhibited deficient DNA damage repair (DDR). Histologically, tumors lacking Lkb1 were enriched in neural‐like tumor morphology. …”
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miR-129-5p Promotes Osteogenic Differentiation of BMSCs and Bone Regeneration via Repressing Dkk3 by Changming Zhao, Yulin Gu, Yan Wang, Qiaozhen Qin, Ting Wang, Meng Huang, Heyang Zhang, Yannv Qu, Jingwen Zhang, Zhangzhen Du, Xiao-Xia Jiang, Lulu Xu

“…For the transfection experiments, lipofectamine 3000 was used to transfect pcDNA-Dkk3 into BMSCs to overexpress Dkk3. Coimmunoprecipitation and immunofluorescent localization assay were included for exploring the role of Dkk3 and β-catenin. …”
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NKAPL facilitates transcription pause-release and bridges elongation to initiation during meiosis exit by Zhenlong Kang, Chen Xu, Shuai Lu, Jie Gong, Ruoyu Yan, Gan Luo, Yuanyuan Wang, Qing He, Yifei Wu, Yitong Yan, Baomei Qian, Shenglin Han, Zhiwen Bu, Jinwen Zhang, Xian Xia, Liang Chen, Zhibin Hu, Mingyan Lin, Zheng Sun, Yayun Gu, Lan Ye

“…NKAPL binds to promoter-associated nascent transcripts and co-localizes with DNA-RNA hybrid R-loop structures at GAA-rich loci to enhance R-loop formation and facilitate Pol II pause-release. …”
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Establishment and characterization of a sigmoid colon cancer organoid with spinal metastasis by Jingyao Chen, Jingyao Chen, Shumin Cheng, Liang Gu, Liang Gu, Zhangsen Huang, Zhangsen Huang, Changhua Zhang, Changhua Zhang, Chunhui Sun, Chunhui Sun, Chunhui Sun, Songyao Chen, Songyao Chen

“…HE staining, immunohistochemistry, and DNA sequencing were utilized to compare the biological characteristics between the original tumor and the organoid. …”
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From bench to bedside: elucidating VEGF(R) inhibitor-related heart failure in cancer treatment by Shengkun Peng, MinHong Cai, Hongyu Kuang, Anqi Lin, Qinghua Ma, Xiaoqin Dai, Peng Luo, Yijun Liu, Guo Zhang, Yifeng Bai

“…Transcriptomic analysis identified significant dysregulation of multiple key signaling pathways, including DNA repair (R = 0.46), mitochondrial ATP synthesis (R = 0.39), glycogen metabolism regulation (R = 0.45), and proteasome-mediated protein degradation (R = 0.45). …”
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Nucleic Acid, Antibody, and Virus Culture Methods to Detect Xenotropic MLV-Related Virus in Human Blood Samples by M. F. Kearney, K. Lee, R. K. Bagni, A. Wiegand, J. Spindler, F. Maldarelli, P. A. Pinto, W. M. Linehan, C. D. Vocke, K. A. Delviks-Frankenberry, R. W. deVere White, G. Q. Del Prete, J. W. Mellors, J. D. Lifson, V. N. KewalRamani, V. K. Pathak, J. M. Coffin, S. F. J. Le Grice

“…Analysis of XMRV-spiked samples and/or specimens from two pigtail macaques experimentally inoculated with 22Rv1 cell-derived XMRV confirmed the ability of the assays used to detect XMRV RNA and DNA, and culture isolatable virus when present, along with XMRV reactive antibody responses. …”
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重组人高密度脂蛋白B族I型清道夫受体基因腺相关病毒血 清型2/1杂合载体的构建及其在HepG2肝细胞的表达

“…【目的】 构建含绿色荧光蛋白(EGFP)重组人高密度脂蛋白B族I型清道夫受体(SR-BI)基因腺相关病毒(rAAV)2/1杂合载体,观察其在HepG2肝细胞的表达65377;【方法】以pCMV.SPORT6-SR-BI质粒为模板PCR扩增目的基因cDNA,将其克隆至pSNAV2.0-IRES-EGFP-LacZa载体中,经酶切及测序鉴定后,脂质体介导转染BHK21细胞,经G418筛选得到含SR-BI基因的BHK/SR-BI-IRES-EGFP载体细胞株,用携带rep2-cap1基因的辅助病毒(rHsv/r2cl)感染该细胞株65377;通过细胞孵育65380;裂解和病毒纯化,得到杂合载体rAAV2/1-SR-BI-IRES-EGFP病毒65377;免疫荧光显微镜检测绿色荧光表达,计算转染效率,Western Blot检测转染后SR-BI蛋白的表达65377; 【结果】 酶切鉴定表明pSNAV2.0-SR-BI-IRES-EGFP重组成功,基因测序显示装入pSNAV2.0-IRES-EGFP-LacZa质粒中的SR-BI基因正确;成功构建了rAAV2/1-SR-BI-IRES-EGFP病毒载体65377;转染HepG2肝细胞后免疫荧光显微镜检测最佳MOI值为1 × 105时, 以此值转染HepG2肝细胞荧光高效表达; Western Blot检测未转染组及rAAV2/1-IRES-EGFP组HepG2肝细胞均有SR-BI蛋白表达,rAAV2/1-SR-BI-IRES-EGFP组SR-BI蛋白表达水平较rAAV2/1-IRES-EGFP组及未转染组显著增加65377;【结论】 成功构建了rAAV2/1-SR-BI-IRES-EGFP杂合载体系统,转染后SR-BI蛋白在HepG2肝细胞高效表达,为SR-BI生理学作用机制及临床应用的深入研究奠定基础65377;…”
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Toxicity assessment of DMSO extracts of environmental aged beached plastics using human cell lines by Florane Le Bihanic, Bettie Cormier, Emilie Dassié, Sophie Lecomte, Justine Receveur, Stéphane Le Floch, Jérôme Cachot, Bénédicte Morin

“…PVC was the most toxic plastic inducing cytotoxicity for both cell lines. DNA damage was observed for Caco-2 cells exposure to HDPE, PVC and nylon. …”
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SRT3025-loaded cell membrane hybrid liposomes (3025@ML) enhanced anti-tumor activity of Oxaliplatin via inhibiting pyruvate kinase M2 and fatty acid synthase by Xiaobin Wang, Shulin Li, Zichen Li, Zhuona Lin, Zhifeng Wang

“…This novel drug delivery system inhibits key metabolic pathways, which increase DNA damage and tumor cell apoptosis. The results indicate that 3025@ML is a promising therapeutic strategy for overcoming OXA’s compromised therapeutic effect and potentially improving cancer treatment outcomes.…”
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DDX18 coordinates nucleolus phase separation and nuclear organization to control the pluripotency of human embryonic stem cells by Xianle Shi, Yanjing Li, Hongwei Zhou, Xiukun Hou, Jihong Yang, Vikas Malik, Francesco Faiola, Junjun Ding, Xichen Bao, Miha Modic, Weiyu Zhang, Lingyi Chen, Syed Raza Mahmood, Effie Apostolou, Feng-Chun Yang, Mingjiang Xu, Wei Xie, Xin Huang, Yong Chen, Jianlong Wang

“…Using techniques such as Hi-C, DNA/RNA-FISH, and biomolecular condensate analysis, we demonstrate that DDX18 regulates nucleolus phase separation and nuclear organization by interacting with NPM1 in the granular nucleolar component, driven by specific nucleolar RNAs. …”
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RETRACTED ARTICLE: P16INK4a Deletion Ameliorated Renal Tubulointerstitial Injury in a Stress-induced Premature Senescence Model of Bmi-1 Deficiency by Jianliang Jin, Jianguo Tao, Xin Gu, Zhenzhen Yu, Rong Wang, Guoping Zuo, Qing Li, Xianhui Lv, Dengshun Miao

“…Our results demonstrated that p16INK4a deletion largely rescued renal aging phenotypes caused by Bmi-1 deficiency, including impaired renal structure and function, decreased proliferation, increased apoptosis, senescence and SASP, DNA damage, NF-κB and TGF-β1/Smad signal activation, inflammatory cell infiltration, and tubulointerstitial fibrosis and tubular atrophy. …”
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The impact of an integrated intervention program combining drug therapy with water, sanitation, and hygiene (WASH) education on reinfection with intestinal parasitic infections amo... by Woottichai Nachaiwieng, Sangob Sanit, Nattharinee Kongta, Jassada Saingamsook, Suwit Duangmano, Sakorn Pornprasert, Pradya Somboon, Jintana Yanola

“…Detection of benzimidazole resistance linked to the beta-tubulin gene mutation in soil-transmitted helminths (STH) was performed using polymerase chain reaction (PCR) amplification and DNA sequencing. Mass drug administration (MDA) with albendazole was applied to both groups. …”
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