Impact of Improved Sanitation Standard Operating Procedures on Microbial Populations at Three Tree Fruit Packing Facilities by M. Laura Rolon, Marysabel Mendez Acevedo, Priscilla Sinclair, Dumitru Macarisin, Luke F. LaBorde, Jasna Kovac

“…We observed inconsistent changes in the bacterial and fungal microbiota composition due to the application of cleaning and sanitizing SSOPs, which may be due to the presence of dead DNA after the treatment. Using Nanopore sequencing, we detected functional elements related to biofilm formation and stress resistance in the microbiomes of the studied environments. …”
Get full text

Microsatellite instability and somatic gene variant profile in solid organ tumors by Ibrahim Halil Erdogdu, Seda Orenay-Boyacioglu, Olcay Boyacioglu, Nesibe Kahraman-Cetin, Habibe Guler, Merve Turan, Ibrahim Meteoglu

“…Introduction Absence of mismatch repair (MMR) genes in tumor cells or errors in the replication repair process may lead to DNA-MMR deficiency and microsatellite instability (MSI) formation. …”
Get full text

Dynamic transitions of initiator binding coordinate the replication of the two chromosomes in Vibrio cholerae by Théophile Niault, Ariel Talavera, Eric Le Cam, Sonia Baconnais, Ole Skovgaard, Florian Fournes, Léa Wagner, Hedvig Tamman, Andrew Thompson, Dannele Echemendia-Blanco, Noa Guzzi, Abel Garcia-Pino, Didier Mazel, Marie-Eve Val

“…Structural analyzes indicate the formation of diverse oligomeric states of RctB, coupled to the allosteric effect of DNA, which determine ori2 accessibility. We propose a synchronization model where, upon replication, crtS locally destabilizes the RctB inhibition complex, releasing the Chr2 replication origin.…”
Get full text

The β-Lactamase Gene Profile and a Plasmid-Carrying Multiple Heavy Metal Resistance Genes of Enterobacter cloacae by Chongyang Wu, Chaoqin Lin, Xinyi Zhu, Hongmao Liu, Wangxiao Zhou, Junwan Lu, Licheng Zhu, Qiyu Bao, Cong Cheng, Yunliang Hu

“…In this work, by high-throughput sequencing, antibiotic resistance genes, including class A (blaCTX-M, blaZ, blaTEM, blaVEB, blaKLUC, and blaSFO), class C (blaSHV, blaDHA, blaMIR, blaAZECL-29, and blaACT), and class D (blaOXA) β-lactamase genes, were identified among the pooled genomic DNA from 212 clinical Enterobacter cloacae isolates. …”
Get full text

Fusobacterium nucleatum upregulates the immune inhibitory receptor PD-L1 in colorectal cancer cells via the activation of ALPK1 by Coco Duizer, Moniek Salomons, Merel van Gogh, Sanne Gräve, Freke A. Schaafsma, Maaike J. Stok, Merel Sijbranda, Raghuvandhanan Kumarasamy Sivasamy, Rob J. L. Willems, Marcel R. de Zoete

“…By performing transcriptional analysis of ADP-heptose stimulated HT-29 cells, we found several inflammatory and cancer-related pathways to be differentially regulated, including DNA mismatch repair genes and the immune inhibitory receptor PD-L1. …”
Get full text

干扰素调节因子5下调PARP-1抑制鼻咽癌细胞的侵袭力 by 李民英, 何秀芳, 郑斯明, 周嘉雄

“…【目的】探讨干扰素调节因子5（IFR5）是否通过下调DNA修复酶PARP-1，进而抑制鼻咽癌细胞的侵 袭能力。【方法】本研究使用的临床标本是经病理确诊的鼻咽癌患者组织标本46例和正常组织51例。…”
Get full text

The effect of <i>T. aestivum</i> chromosomes 1A and 1D on fertility of alloplasmic recombinant (<i>H. vulgare</i>)-<i>T. aestivum</i> lines depending on cytonuclear compatibility by L. A. Pershina, N. V. Trubacheeva, V. K. Shumny

“…Heteroplasmy (simultaneous presence of barley and wheat mtDNA copies) was found in FS, PS, PF and some F plants, which was associated with a violation of cytonuclear compatibility. …”
Get full text

Protocol for faecal microbiota transplantation in irritable bowel syndrome: the MISCEAT study – a randomised, double-blind cross-over study using mixed microbiota from healthy dono... by Lenka Kramna, Ondrej Cinek, Jakub Hurych, Jiri Vejmelka, Lucie Hlinakova, Vladyslav Larionov, Michal Kulich, Pavel Kohout

“…Faecal bacteriome will be profiled before and regularly after interventions using 16S rDNA next-generation sequencing. Food records, dietary questionnaires, anthropometry, bioimpedance, biochemistry and haematology workup will be obtained at study visits during the follow-up period. …”
Get full text

The Mla pathway promotes Vibrio cholerae re-expansion from stationary phase by Deborah R. Leitner, Franz G. Zingl, Alexander A. Morano, Hailong Zhang, Matthew K. Waldor

“…Here, using the cholera pathogen, we uncovered a role for the Mla pathway, a system that enables phospholipid re-cycling, in promoting Vibrio cholerae re-expansion from stationary phase cultures. Cells labeled with DNA barcodes or fluorophores were useful to demonstrate that though the abundances of wild-type and Mla mutant cells were similar in stationary phase cultures, they had marked differences in their capacities to regrow on plates. …”
Get full text

NAD⁺激活SIRT3/IDH2信号通路改善高血压血管内皮功能障碍 by 丘雨旻, 陈曦, 张建宁, 刘张弛, 朱秋霞, 张美信, 陶军, 吴杏

“…采用迁移实验和划痕实验评估体外内皮细胞功能；采用免疫荧光染色法检测mtROS水平；采用RT-PCR检测mtDNA、SIRT3、异柠檬酸脱氢酶2（IDH2）的mRNA表达；采用酶联免疫吸附法检测8-羟基-2'-脱氧鸟苷的水平；采用Western blot法检测p-eNOS、eNOS、SIRT3、IDH2蛋白表达水平。…”
Get full text

Interactions of miR-34b/c and TP53 Polymorphisms on the Risk of Intracranial Aneurysm by Lijuan Li, Xiutian Sima, Peng Bai, Lushun Zhang, Hong Sun, Weibo Liang, Jianxing Liu, Lin Zhang, Linbo Gao

“…., miR-34b/c rs4938723 and TP53 Arg72-Pro) were genotyped by polymerase chain reaction-restriction fragment length polymorphism assay and DNA sequencing. We found that the CC genotype of miR-34b/c rs4938723 was significantly associated with a decreased risk of IA compared with the TT genotype. …”
Get full text

Malignant phyllodes tumors with sarcomatous components: A histopathologic and molecular study by Ting Lei, Yunjie Song, Zhiyi Shen, Yongqiang Shi, Cunyan Xia, Xu Deng, Wenyue Da, Yan Peng, Qing Li

“…Methods: A total of ten cases were collected with eight cases undergoing DNA next-generation sequencing (NGS). The clinicopathological characteristics, prognostic information, and genomic profiles were compared to those of MPTs without sarcomatous components. …”
Get full text

Exploiting somatic oncogenic driver alterations in a patient with Li-Fraumeni syndrome– paving the path towards precision medicine: a case report by Carolin Seeling, Sonja Dahlum, Ralf Marienfeld, Vera Jan, Brigitte Rack, Uwe Gerstenmaier, Ambros J. Beer, Regine Mayer-Steinacker, Wolfgang Thaiss, Thomas F. E. Barth, Thomas Seufferlein, Nadine T. Gaisa, Stephan Stilgenbauer, Wolfgang Janni, Reiner Siebert, Hartmut Döhner, Verena I. Gaidzik

“…It results from pathogenic/likely pathogenic heterozygous constitutional variants of the TP53 gene. Due to impaired DNA damage repair, conventional cytotoxic therapies or radiotherapy should be avoided whenever feasible to mitigate the high incidence of treatment-related secondary malignancies in these patients. …”
Get full text

Constructing the constitutively active ribosomal protein S6 kinase 2 from <i>Arabidopsis thaliana</i> (AtRPS6K2) and testing its activity <i>in vitro</i> by A. V. Zhigailov, G. E. Stanbekova, D. K. Beisenov, A. S. Nizkorodova, N. S. Polimbetova, B. K. Iskakov

“…We have cloned AtRPS6K2 cDNA gene and carried out in vitro mutagenesis replacing codons encoding S296, S437 and T455 by triplets of phosphomimetic glutamic acid (E). …”
Get full text

Development and progression association of various ophthalmic pathologies with the gut microbiome state (literature review) by G.N. Rezbaeva, A.E. Babushkin, O.I. Orenburkina, I.A. Gimranova

“…A huge study breakthrough of the gut microbiota species composition in a healthy person, as well as in persons with various pathologies, was obtained after a technique development for DNA sequencing. Modern researches focus on studying the effect of changes in the gut microbiome on human health, including his eyes. …”
Get full text

Immune-endocrine crossroads: the impact of nuclear receptors in Tuberculosis and Chagas disease by Ana R. Pérez, Ana R. Pérez, Oscar A. Bottasso, Oscar A. Bottasso, Oscar A. Bottasso, Natalia E. Santucci, Natalia E. Santucci

“…They act as transcription factors and are subclassified into families based on their ligands, DNA-binding sequences, tissue specificity, and functions. …”
Get full text

Valorization of vermicompost with bacterial fermented chicken feather hydrolysate for the yield improvement of tomato plant: A novel organic combination by Ishita Biswas, Debasis Mitra, Ansuman Senapati, Debanjan Mitra, Sourav Chattaraj, Murshed Ali, Goutam Basak, Periyasamy Panneerselvam, Pradeep K. Das Mohapatra

“…Method Feather degrading bacteria were isolated and characterized using 16s-rDNA sequencing, and assessed for biochemical reactions, growth-promoting attributes and keratinase activity. …”
Get full text

Ru(II) Complexes Bearing O, O-Chelated Ligands Induced Apoptosis in A549 Cells through the Mitochondrial Apoptotic Pathway by Jincan Chen, Jie Wang, Yuanyuan Deng, Tao Wang, Tifang Miao, Chengpeng Li, Xianhong Cai, Ying Liu, Justin Henri, Lanmei Chen

“…Further studies revealed that these complexes could induce apoptosis in A549 cells, including activating caspase family proteins and poly (ADP-ribose) polymerase (PARP), reducing Bcl-2/Bax and Bcl-xl/Bad ratio, enhancing cellular reactive oxygen species (ROS) accumulation, triggering DNA damage, decreasing mitochondrial membrane potential (MMP), and leading cytochrome c release from mitochondria. …”
Get full text

中国南方一罕见迟发性脊椎骨骺发育不良大家系的表型和基因型的相关性研究 by 黄颖, 谢杰, 郑诗瑶, 唐佳, 郭奕斌

“…方法在临床初诊、系谱分析的基础上，首先采用高通量测序技术对先证者及其家庭核心成员的DNA样品进行全外显子组测序。通过生信分析找出候选基因，在确定候选基因及其新变异后，采用PCR、Sanger测序、RT-PCR、q-PCR等方法对该新变异的致病性进行系列鉴定。…”
Get full text

Methionine-driven methylation modification overcomes plasmid-mediated high-level tigecycline resistance by Dan Fang, Tianqi Xu, Fulei Li, Yue Sun, Jingyi Sun, Yanqing Yin, Haijie Zhang, Zhiqiang Wang, Yuan Liu

“…Consistently, the potentiation of Met to tigecycline is abolished in tet(X4)-carrying E. coli Δdcm but restored in dcm-complementary bacteria, which encodes DNA-cytosine methyltransferase. In multiple animal models of infection, Met markedly potentiates the effectiveness of tigecycline against pathogenic E. coli and K. pneumoniae. …”
Get full text