Impact of meropenem exposure on fluoroquinolone and carbapenem resistance in Pseudomonas aeruginosa infection in inpatients in a Japanese university hospital: Insights into oprD mu... by Tadanori Yamochi, Kazuhisa Ugajin, Rintaro On, Sho Inoue, Hiromi Ikeda, Toshiko Yamochi, Masafumi Takimoto, Issei Tokimatsu

“…Quantitative PCR was performed to analyze oprD, mexB, mexC, mexE, and mexY expression. DNA sequencing was performed to identify mutations. …”
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Transcriptomic and proteomic profiling identifies feline fibrosarcoma as clinically amenable model for aggressive sarcoma subtypes by Mikiyo Weber, Daniel Fuchs, Amiskwia Pöschel, Erin Beebe, Zuzana Garajova, Armin Jarosch, Laura Kunz, Witold Wolski, Lennart Opitz, Franco Guscetti, Mirja C. Nolff, Enni Markkanen

“…Significant enrichment in DNA repair pathways in feline FSA correlate with aggressive clinical behavior in human soft-tissue sarcoma. …”
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组蛋白去乙酰化酶抑制剂通过下调MCM2-7表达抑制神经胶质瘤细胞增殖 by 李慧锋, 袁忠民, 吴森斌, 马莹, 赵凡一, 何伟文, 梁建峰, 伍健伟

“…用组蛋白去乙酰化酶抑制剂：包括帕比司他和曲古霉素（TSA）处理U251细胞后，BrdU掺入实验检测细胞增殖和DNA复制能力。用MCM抑制剂环丙沙星（CPX）处理U251和H4细胞，MTT法检测细胞存活率，流式细胞术检测细胞周期进程改变。…”
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Evaluation of X-Inactivation Status and Cytogenetic Stability of Human Dermal Fibroblasts after Long-Term Culture by Zhi-Gang Xue, Zhan-Ping Shi, Juan Dong, Ting-Ting Liao, Yan-Peng Wang, Xue-Ping Sun, Zheng-Jie Yan, Xiao-Qiao Qian, Yu-Gui Cui, Juan Chen, Jia-Yin Liu, Guoping Fan

“…Our data suggest that the copy number of inactive X chromosomes in a subset of female HDF is increased by a two-fold. Consistently, DNA fluorescent in situ hybridization (FISH) identified 3-4 copies of X chromosomes in one nucleus in this subset of cells with two inactive Xs. …”
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Epigenetic alterations of 11beta-hydroxysteroid dehydrogenase 1 gene in the adipose tissue of patients with primary aldosteronism by Yoshimichi Takeda, Masashi Demura, Mitsuhiro Kometani, Shigehiro Karashima, Daisuke Aono, Seigo Konishi, Shin-ichi Horike, Makiko Meguro-Horike, Takashi Yoneda, Yoshiyu Takeda

“…The mRNA level of HSD11B1 was quantified using real-time PCR. Isolated DNA was treated with bisulfite and amplified using primers specific to the human HSD11B1 promoter region. …”
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Harmful impacts of microplastic pollution on poultry and biodegradation techniques using microorganisms for consumer health protection: A review by Mohamed E. Abd El-Hack, Elwy A. Ashour, Fatemah AlMalki, Asmaa F. Khafaga, Mahmoud Moustafa, Mohammed O. Alshaharni, Islam M. Youssef, Ahmed A. Elolimy, Sylwester Świątkiewicz

“…In humans, exposure to microplastics can lead to inflammation, immune responses, metabolic disturbances, DNA damage, neurological damage, and even cancer upon contact with mucosal membranes or absorption into the body. …”
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SUMO modified ETV1 promotes M2-polarized tumor-associated macrophage infiltration and cancer progression by facilitating CCL2 transcription in esophageal squamous cell carcinoma ce... by Tianci Han, Xiaoqi Guo, Junwei Xie, Wei Tong, Liang Zhang

“…Transcriptional activity was measured using dual-luciferase assay, chromatin immunoprecipitation (ChIP) assay and DNA pull-down assay. Results In this study, through analysis of ESCC samples from TCGA database and the clinic, we noticed that ETV1 was highly expressed in ESCC tumor tissues and was associated with TAM infiltration. …”
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Human blood cell transcriptomics unveils dynamic systemic immune modulation along colorectal cancer progression by Pedro J Romero, Mauro Delorenzi, Amaia Martínez-Usatorre, Laura Ciarloni, Noushin Hadadi, Eric Durandau, Sylvain Monnier-Benoit, Paolo Angelino, Victoria Wosika, Alessandra Jordano Conforte, Sara S Fonseca Costa, Hector Fabio Satizabal, Jérémie Despraz, Andres Perez-Uribe, Stephan Morgenthaler, Brian Hashemi, Sahar Hosseinian-Ehrensberger

“…CRC deaths can be reduced with prevention and early diagnosis. Circulating tumor DNA-based liquid biopsies, are emerging tools for cancer detection. …”
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Argonaute2 and Argonaute4 Involved in the Pathogenesis of Kawasaki Disease via mRNA Expression Profiles by Zon-Min Lee, Hui-Chuan Chang, Shih-Feng Liu, Ying-Hsien Huang, Ho-Chang Kuo

“…These proteins consist of small, single-stranded RNA or DNA and may provide a route for detecting and silencing complementary mobile genetic elements. …”
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Anatomical, subset, and HIV-dependent expression of viral sensors and restriction factors by Ashley F. George, Jason Neidleman, Xiaoyu Luo, Julie Frouard, Natalie Elphick, Kailin Yin, Kyrlia C. Young, Tongcui Ma, Alicer K. Andrew, Ifeanyi J. Ezeonwumelu, Jesper G. Pedersen, Antoine Chaillon, Magali Porrachia, Brendon Woodworth, Martin R. Jakobsen, Reuben Thomas, Davey M. Smith, Sara Gianella, Nadia R. Roan

“…Mucosal myeloid cells are well equipped with SAMHD1 and sensors of viral capsid and DNA while CD4+ T cells are not. In lymph node CD4+ Tfh, VISOR expression patterns reflect those favoring integration but blocking HIV gene expression, thus favoring viral latency. …”
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Analysis of the structure and function of the tomato <i>Solanum lycopersicum</i> L. MADS-box gene <i>SlMADS5</i> by A. V. Nezhdanova, M. A. Slugina, E. A. Dyachenko, A. M. Kamionskaya, E. Z. Kochieva, A. V. Shchennikova

“…Transgenic plants Nicotiana tabacum L. with constitutive overexpression of SlMADS5 cDNA flowered 2.2 times later than the control; plants formed thickened leaves, 2.5–3.0 times thicker stems, 1.5–2.7 times shortened internodes, and 1.9 times fewer flowers and capsules than non-transgenic plants. …”
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Unravelling genomic drivers of speciation in Musa through genome assemblies of wild banana ancestors by Guillaume Martin, Benjamin Istace, Franc-Christophe Baurens, Caroline Belser, Catherine Hervouet, Karine Labadie, Corinne Cruaud, Benjamin Noel, Chantal Guiougou, Frederic Salmon, Joël Mahadeo, Fajarudin Ahmad, Hugo A. Volkaert, Gaëtan Droc, Mathieu Rouard, Julie Sardos, Patrick Wincker, Nabila Yahiaoui, Jean-Marc Aury, Angélique D’Hont

“…Comparative and phylogenetic analyses reveal an ongoing speciation process within Musa, characterised by large chromosome rearrangements and centromere differentiation through the integration of different types of repeated sequences, including rDNA tandem repeats. This speciation process may have been favoured by reproductive isolation related to the particular context of climate and land connectivity fluctuations in the Southeast Asian region.…”
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Development of novel nucleic acid therapy aimed at directly controlling liver fibrosis by Tomohiro Umezu, Masakatsu Takanashi, Koji Fujita, Akio Ishikawa, Yuichirou Harada, Yoshinari Matsumoto, Masahiko Kuroda, Yoshiki Murakami

“…The original microRNA was changed to an RNA-DNA hybrid structure: the 2′ position of the RNA was modified with a fluorine base, and locked nucleic acid and phosphorothioate were crosslinked (hereafter called modified nucleic acid). …”
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Genetic Diversity, Repeat Motifs, and Natural Selection at the C-Terminal Knob-Associated Histidine Rich Protein (KAHRP) of Plasmodium falciparum Clinical Samples from Saudi Arabia by Saad M. Bin Dajem, Md Atique Ahmed, Marie Fe F Bohol, Syeda Wasfeea Wazid, Mohammed I. Shafeai, Fuad H. Rudiny, Ali M. Motaen, Kareem Morsy, Hani Alothaid, Ahmed A. Al-Qahtani

“…The PfKHARP C-terminal interaction domain was successfully PCR-amplified and sequence data from 441 clinical isolates from Saudi Arabia were obtained. The DnaSP v5.10 software was used to determine the genetic diversity, polymorphism, haplotype, and natural selection. …”
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Bacterial community profiling and identification of bacteria with lignin-degrading potential in different gut segments of African palm weevil larvae (Rhynchophorus phoenicis) by Jessica Lenka, Jessica Lenka, Enrique González-Tortuero, Shweta Kuba, Natalie Ferry

“…In this study, we aimed to explore the bacterial community within the gut of the APW larvae, specifically with respect to the potential for lignin degradation in various gut segments as a first step to determining the viability of mining bacterial lignin-degrading enzymes for the bioconversion of lignocellulosic biomass to biofuels and biomaterials. Bacterial metagenomic DNA was extracted from the foregut, midgut, and hindgut of larvae of the APW, and the V3–V4 hypervariable region of the 16S rRNA gene was sequenced using the Illumina MiSeq platform. …”
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Expression of angiotensin converting enzyme 2 in patients with oral squamous cell carcinoma by Shivani Sivasakthivel, Pratibha Ramani, Selvaraj Jayaraman

“…RNA isolation was performed using the TRIR kit from Invitrogen. cDNA was synthesized from total RNA isolated from tissues using a first-strand synthesis kit. …”
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Determinants of survival of the bovine blastocyst to cryopreservation stress: treatment with colony stimulating factor 2 during the morula-to-blastocyst transition and embryo sex by Froylan Sosa, Jeremy Block, Yao Xiao, Peter J. Hansen

“…In the first experiment, blastocysts were vitrified, warmed, cultured for 24 h, and DNA embryo sexing performed by PCR. There was no effect of CSF2, sex, or the interaction on the percent of blastocysts that re-expanded or that were hatching or hatched. …”
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Polymorphisms and dental age in non-syndromic cleft lip and palate: a cross-sectional study by Gabriela Fonseca-Souza, Lhorrany Alves-Souza, Maria Angélica Hueb de Menezes-Oliveira, Nikolaos Daratsianos, Svenja Beisel-Memmert, Christian Kirschneck, Rafaela Scariot, Juliana Feltrin-Souza, Erika Calvano Küchler

“…DA was evaluated by calibrated examiners (kappa > 0.80) using the method proposed by Demirjian et al. (1973). Genomic DNA was extracted from buccal cells, and SNPs in Epidermal Growth Factor (EGF) – rs4444903 and rs2237051, Epidermal Growth Factor Receptor (EGFR) – rs2227983 –, Transforming Growth Factor Beta 1 (TGFB1) – rs1800470 and rs4803455 –, and Transforming Growth Factor Beta Receptor 2 (TGFBR2) – rs3087465 – were genotyped by real-time polymerase chain reactions using the TaqMan assay. …”
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Exposure of Apis mellifera (Hymenoptera: Apidae) colonies to imidacloprid impairs larval development, promotes oxidative stress in pupae, and induces changes in the midgut of adult... by Daiani Rodrigues Moreira, Tuan Henrique Smielevski de Souza, Douglas Galhardo, Cinthia Leão Figueira, Samara Calvi Baulli, Breno Gabriel da Silva, Francieli das Chagas, José Washington Santos Oliveira, Jean Samel Rocha, Angélica de Souza Khatlab, Eliane Gasparino, Vagner de Alencar Arnaut de Toledo, Adriana Aparecida Sinópolis Gigliolli, Maria Claudia Colla Ruvolo-Takasusuki

“…Oxidative stress, which was detected at different stages of bee development, may induce lipid, protein, and DNA oxidation, leading to cell death. Graphical Abstract…”
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The ILE462VAL polymorphism of the cytochrome P450 CYP1A1 gene among Tundra Nenets in Yamalo-Nenets Autonomous Okrug, Nganasans in the Taimyr Peninsula and Russians in Siberia by R. P. Tiis, L. P. Osipova, T. V. Churkina, L. E. Tabikhanova, D. V. Lichman, E. N. Voronina, M. L. Filipenko

“…This substitution results in a two- fold increase in enzyme activity, which leads to accumulation of active intermediates and increases the risk of DNA mutations and chemically induced carcinogenesis. …”
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