ASSOCIATION BETWEEN POLYMORPHISMS IN GENES ENCODING 2′-5′-OLIGOADENYLATE SYNTHETASES AND THE HUMORAL IMMUNE RESPONSE UPON VACCINATION AGAINST TICK-BORNE ENCEPHALITIS by N. S. Yudin, A. V. Igoshin, S. L. Lutova, Ya. Gon, M. I. Voevoda, V. A. Belyavskaya

“…Levels of specific IgG antibodies against tick-borne encephalitis virus and interleukin 4 (IL-4) were analyzed. Genomic DNA samples were genotyped for the SNPs rs2285932, rs2072136, rs1293762, rs15895 and rs1732778 in genes encoding 2’-5’-oligoadenylate synthetases OAS2 and OAS3. …”
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Auditory Phenotype of a Novel Missense Variant in the CEACAM16 Gene in a Large Russian Family With Autosomal Dominant Nonsyndromic Hearing Loss by Tatiana G. Markova, Natalia N. Alekseeva, Oxana P. Ryzhkova, Olga L. Shatokhina, Anna A. Orlova, Viktoriia V. Zabnenkova, Olga S. Groznova, Olesya V. Sagaydak, Svetlana S. Chibisova, Alexander V. Polyakov, George A. Tavartkiladze

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DRAM1 enhances the proliferation and metastasis of gastric cancer through the PI3K/AKT/mTOR signaling pathway and energy metabolism by Xinrong Wu, Yifan Li, Weiwei Wang, Jiale Xu, Bei Zhao, Wenqi Sun, Dan Ge, Longying Xiong, Xiaotan Dou, Xiaoping Zou, Lei Wang, Min Chen

“…Metabolic reprogramming, a hallmark of tumors, plays a pivotal role in tumor progression, immune evasion, and immune surveillance. DNA damage-regulated autophagy modulator 1 (DRAM1) encodes a hexameric transmembrane protein that is predominantly located in lysosomes and induces autophagy; however, its mechanism of action in gastric cancer remains unclear. …”
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Imaging Flow Cytometric Identification of Chromosomal Defects in Paediatric Acute Lymphoblastic Leukaemia by Ana P. A. Simpson, Carly E. George, Henry Y. L. Hui, Ravi Doddi, Rishi S. Kotecha, Kathy A. Fuller, Wendy N. Erber

“…., expression of CD34, CD10, and CD19 antigens), we assessed for copy numbers of loci for the centromeres of chromosomes 4 and 21 and the <i>ETV6</i> and <i>RUNX1</i> regions using fluorophore-labelled DNA probes in more than 1000 cells per sample. Trisomy 4 and 21, tetrasomy 21, and translocations of <i>ETV6::RUNX1,</i> as well as gains and losses of <i>ETV6</i> and <i>RUNX1,</i> could all be identified based on FISH spot counts and digital imagery. …”
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Identification of core biomarkers for tuberculosis progression through bioinformatics analysis and in vitro research by Zhanpeng Chen, Qiong Wang, Quan Ma, Jinyun Chen, Xingxing Kong, Yuqin Zeng, Lanlan Liu, Shuihua Lu, Xiaomin Wang

“…Gene Ontology (GO) enrichment analysis revealed that the biological functions of these genes mainly involve DNA translation, RNA processing, and transcriptional regulation. …”
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Dissecting the role of single nucleotide polymorphism of lymphotoxin beta gene during pig domestication using bioinformatic and experimental approaches by R. B. Aitnazarov, E. V. Ignatieva, N. E. Bazarova, V. G. Levitsky, S. P. Knyazev, Y. Gon, N. S. Yudin

“…The rs340283541 SNP occurs in a DNA motif highly conservative among 11 mammalian species; thus, it may be of functional significance. …”
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Invasive mealybug Pseudococcus jackbeardsleyi Gimpel and Miller on Curcuma aeruginosa Roxb. on the seed rhizome in storage: a record of a new host by Rohimatun, Nida Nur Rahmah, Dewi Sartiami, Mahindra Dewi Nur Aisyah, Miftakhurohmah Miftakhurohmah, Melati, Devi Rusmin, Tri Lestari Madiningsih

“…Amplification of the COI gene yielded DNA bands measuring 490 base pairs (bp), while homology and phylogeny analysis confirmed the morphological identification. …”
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Prevalence and investigation of Cytomegalovirus (HCMV) in blood donors from the main blood establishment in Rio de Janeiro/Brazil by Agildo da Silva Oliveira, Jéssica Gonçalves Pereira, Gabrielle Tantos Nunes, Ivanildo Pedro de Sousa Junior, Dmitry José de Santana Sarmento, Josiane Iole França Lopes, Luiz Amorim Filho, Vanessa Salete de Paula

“…The prevalence of cytomegalovirus was 88.5 %, and HCMV DNA was detected in 1.2 % of the samples. Discussion: Given that there are approximately 100,000 blood donations per year, this prevalence rate is considerably high compared to that in developed countries. …”
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Liver Expression of Sulphotransferase 2A1 Enzyme Is Impaired in Patients with Primary Sclerosing Cholangitis: Lack of the Response to Enhanced Expression of PXR by Ewa Wunsch, Marta Klak, Urszula Wasik, Malgorzata Milkiewicz, Malgorzata Blatkiewicz, Elzbieta Urasinska, Olivier Barbier, Dariusz Bielicki, Dimitrios P. Bogdanos, Elwyn Elias, Piotr Milkiewicz

“…PXR and SULT2A1 mRNA was analyzed in intestinal tissues from 22 PSC patients. Genomic DNA was isolated from blood of PSC patients (n=120) and an equal number of healthy volunteers. …”
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Detection of Bartonella spp. in foxes' populations in Piedmont and Aosta Valley (NW Italy) coupling geospatially-based techniques by Annalisa Viani, Tommaso Orusa, Sara Divari, Stella Lovisolo, Stefania Zanet, Riccardo Orusa, Enrico Borgogno-Mondino, Enrico Bollo

“…A total of 114 spleen samples were collected from hunted red foxes and screened for Bartonella spp. DNA using a qPCR assay targeting the ssrA locus. …”
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Ficus deltoidea standardized methanolic extract improves kidney structure and function against streptozotocin-induced diabetic nephropathy in rats by Nurdiana Samsulrizal, Noor Syaffinaz Noor Mohamad Zin, Nur Syimal`ain Azmi, Mohd Daud Bakar, Hafandi Ahmad, Yong-Meng Goh

“…We noticed that biochemical and pathological changes corresponded to the infrared (IR) peaks at 1545 cm-1 and 1511 cm-1 (amide II), 1246 cm-1 (DNA/RNA phosphate backbones), and 1181 cm-1 (C–O vibrations). …”
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Association between Genetic Instability and Helicobacter pylori Infection in Gastric Epithelial Dysplasia by Jin Su Kim, Woo Chul Chung, Kang-Moon Lee, Chang Nyol Paik, Kyeong Soo Lee, Hye Ji Kim, Young Wook Kim, Ji Han Jung, Seung June Noh, Yun Kyung Lee

“…In gastric carcinogenesis, changes of DNA methylation appear to be an early molecular event, and the genome-wide methylation state is closely correlated with the level of long interspersed nucleotide element-1 (LINE-1) methylation. …”
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The Polymorphism of Voltage-Gated Sodium Channel Gene in Culex Mosquitoes in Banyuwangi District, Indonesia by Yasmin Azzahra Arifin, Hanifah Dzakiyah, Catherine Canina Charunita, Sakura Alya Ashari, Winna Putri Veliarta, Muhammad Akbar Panjinegara, Francesca Giovanetti, Carlo Montagna, Wojciech Jankowski, Muhammad Fathoni, Binarri Augustya, Lynda Rossyanti, Suhintam Pusarawati, Sukmawati Basuki

“…Culex mosquitoes were identified by morphological examination and Culex DNA was extracted by Chelex100 from each Culex mosquito. …”
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Single nucleotide polymorphism-based visual identification of Rhodiola crenulata using the loop-mediated isothermal amplification technique by Li Hao, Xin Shi, Shiyu Wen, Caiye Yang, Yaqi Chen, Samo Yue, Jiaqiang Chen, Kexin Luo, Bingliang Liu, Yanxia Sun, Yi Zhang

“…The final detection system exhibited a sensitivity of up to 1,000 copies of the target DNA, maintaining a constant reaction temperature of 62°C within 35 minutes. …”
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TGFβ1-Induced Differentiation of Human Bone Marrow-Derived MSCs Is Mediated by Changes to the Actin Cytoskeleton by Mona Elsafadi, Muthurangan Manikandan, Sami Almalki, Mohammad Mobarak, Muhammad Atteya, Zafar Iqbal, Jamil Amjad Hashmi, Sameerah Shaheen, Nehad Alajez, Musaad Alfayez, Moustapha Kassem, Raed Abu Dawud, Amer Mahmood

“…To identify the mechanisms underlying this TGFβ-mediated enhancement of lineage commitment, we compared the gene expression profiles of TGFβ1-treated hMSC cultures using DNA microarrays. In total, 1932 genes were upregulated, and 1298 genes were downregulated. …”
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Renal Failure Associated with APECED and Terminal 4q Deletion: Evidence of Autoimmune Nephropathy by Mohammed Al-Owain, Namik Kaya, Hamad Al-Zaidan, Ibrahim Bin Hussain, Hadeel Al-Manea, Hindi Al-Hindi, Shelley Kennedy, M. Anwar Iqbal, Hamad Al-Mojalli, Albandary Al-Bakheet, Anne Puel, Jean-Laurent Casanova, Saleh Al-Muhsen

“…FISH using a 4p/4q subtelomere DNA probe assay confirmed the deletion of qter subtelomere on chromosome 4. …”
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Identification of HOXA9 methylation as an epigenetic biomarker predicting prognosis and guiding treatment choice in acute myeloid leukemia by Fei Xie, Ting-juan Zhang, Xin-long Zhang, Zi-jun Xu, Liang Qiao, Yun Wang, Yang-jing Zhao, Jun Qian, Jing-dong Zhou

“…However, very few studies determined the DNA methylation-mediated epigenetic regulation of the HOXA cluster genes in AML. …”
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Long-term effect of repeated application of pig slurry digestate on microbial communities in arable soils by Daniela Mora-Salguero, Lionel Ranjard, Thierry Morvan, Samuel Dequiedt, Vincent Jean-Baptiste, Sophie Sadet-Bourgeteau

“…Changes in soil microbial communities were analyzed through molecular microbial biomass and diversity assessments using high-throughput sequencing targeting 16S and 18S ribosomal RNA genes. DNA extraction and molecular analyses were performed on soil samples collected at the start of the trial in 2012 and subsequently in 2017 and 2022. …”
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Barcoded screening identifies nanocarriers for protein delivery to kidney by Luyao Wang, Wen Zhou, Hang Chen, Xiangqian Jia, Peiyuan Zheng, Haolin Jiang, Mengling Wu, Yaning Zhang, Yanchao Ding, Yexi Peng, Rui Zhu, Tiantian Li, Boxue Tian, Bujie Du, Juanjuan Du

“…In this study, we address this challenge by leveraging DNA-barcoded high-throughput screening. We construct a nanocapsule library via in-situ polymerization, incorporating various monomers to create nanocapsules with unique surface properties. …”
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Chromosome-level reference genome assembly for the mountain hare (Lepus timidus) by Fekete, Zsófia, Absolon, Dominic E., Michell, Craig, Wood, Jonathan M. D., Goffart, Steffi, Pohjoismäki, Jaakko L. O.

“…We present here a high-quality genome assembly of a male mountain hare (Lepus timidus Linnaeus), from Ilomantsi, Eastern Finland, utilizing an isolated fibroblast cell line as the source for high quality DNA and RNA. Following the previously published brown hare reference genome assembly, the mountain hare is the second Finnish pilot species for the European Reference Genome Atlas (ERGA) initiative, a collaborative effort to generate reference genomes for European biodiversity. …”
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