Complex immunohistochemical and molecular study on 5 cases of ovarian juvenile granulosa cell tumors reveals a consistent alteration in the PI3K/AKT/mTOR signaling pathway by Adam Šafanda, Nikola Hájková, Michaela Kendall Bártů, Marián Švajdler, Radoslav Matěj, Jitka Hausnerová, Tomáš Zima, Pavel Dundr, Kristýna Němejcová

“…Methods The immunohistochemical examination was performed with 32 markers, including markers that have not been previously investigated. Moreover, DNA next-generation sequencing (NGS) and PTEN methylation analysis was performed. …”
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Beyond Chemistry: Investigating the Physical, Pharmacological, and Computational Aspects of Polyoxometalate Integrated Solid Lipid Nanoparticles for Cancer Treatment by Khan R, Tulain UR, Shah HS, Usman F, Chohan TA, Iqbal J, Kazi M, Ijaz M, Erum A, Malik NS, Mahmood A

“…Further examinations of DNA damage were made through comet test and flow cytometry techniques. …”
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熊猫源唾液乳杆菌的分离鉴定及部分生物学特性分析 by 王黎明 1 张文平 2 王洁 2 谢军金 2 周杰珑 1

“…通过选取健康大熊猫新鲜粪便，利用MRS培养基进行厌氧培养和单克隆纯化，通过形态学、生理生化鉴定、16S rDNA基因序列测定及同源性分析筛选到10株唾液乳杆菌。采用牛津杯法、活菌计数法、药敏纸片法（K B法）和PCR技术对分离菌株作进一步的耐受性、抑菌性和耐药性分析。…”
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标记21和13号染色体双色FISH探针及其在快速产前诊断中的应用

“…【目的】 采用细菌人工染色体(BAC)克隆分别建立21和13号染色体的直标型荧光原位杂交(FISH)探针,并分析其在产前快速诊断21/13号染色体数目异常的可行性65377;【方法】 通过生物信息学网站挑选位于21q22.2(DSCR)和13q14(RB基因)的BAC克隆,培养并提取DNA后采用切口平移法进行红/绿色(21/13)荧光的直接标记65377;取上述探针与中期细胞进行杂交,鉴定探针的杂交位置65377;取10例羊水标本分别采用标记的FISH探针(实验组)与商品化探针(对照组)进行检测,分别计算探针的杂交率与准确率,采用随机化配对设计资料的t检验分析两种探针杂交效能的差异65377;取2008年10月至2010年4月期间,来我院行羊膜腔穿刺术的453例羊水标本作为实验组进行上述探针的FISH分析,同时对该453例标本亦进行细胞培养核型分析作为对照,并将两组检测结果进行比较,验证探针灵敏度65380;特异度及阴65380;阳性预测价值65377;【结果】 采用BAC克隆标记的FISH探针荧光信号清晰65380;明确,与核型分析对照位置吻合;杂交率及准确率与商品化探针相比不存在统计学差异;对453例羊水标本21/13三体进行检测的灵敏度为83.3%(5/6),特异度为100%(447/447),阳性预测价值为100%(5/5),阴性预测价值为99.8%(447/448)65377;【结论】 采用BAC克隆标记建立的21/13号染色体直标型FISH探针荧光信号清晰65380;明确,结果准确可靠65380;成本低廉,可以用于未培养羊水标本的产前快速诊断21/13三体综合征65377;…”
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Antimicrobial Susceptibility Testing of Metronidazole and Clindamycin against Gardnerella vaginalis in Planktonic and Biofilm Formation by Ting Li, Zhan Zhang, Fengjuan Wang, Yuanhui He, Xiaonan Zong, Huihui Bai, Zhaohui Liu

“…Sequence data of clinical G. vaginalis isolates were confirmed by comparing 16S rDNA sequences. Subsequently, clinical isolates were evaluated for antimicrobial susceptibilities in vitro to metronidazole and clindamycin at planktonic and biofilm levels. …”
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CRISPR/Cas12a-based method coupled with isothermal amplification to identify Alternaria spp. isolated from wheat grain samples by Aisha Shaizadinova, Aisha Shaizadinova, Meruyert Amanzholova, Meruyert Amanzholova, Irina Rukavitsina, Sailau Abeldenov, Anuar Rysbekovich Zhumakayev

“…Furthermore, the method exhibited high sensitivity, detecting Alternaria DNA down to 100 copies, validated by real-time fluorescence readout. …”
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Oleanolic Acid Induces Differentiation of Neural Stem Cells to Neurons: An Involvement of Transcription Factor Nkx-2.5 by You Ning, Jianhua Huang, Bill Kalionis, Qin Bian, Jingcheng Dong, Junzhen Wu, Xiantao Tai, Shijin Xia, Ziyin Shen

“…Immunofluorescence analysis showed OA significantly elevated the percentage of TuJ1-positive cells and reduced GFAP-positive cells. Using DNA microarray analysis, 183 genes were differentially regulated by OA. …”
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Infection and HLA-G Molecules in Nasal Polyposis by Roberta Rizzo, Nicola Malagutti, Daria Bortolotti, Valentina Gentili, Antonella Rotola, Enrico Fainardi, Teresa Pezzolo, Claudia Aimoni, Stefano Pelucchi, Dario Di Luca, Antonio Pastore

“…We analyzed the presence of high- and low-risk HPV DNA and the expression of membrane HLA-G (mHLA-G) and IL-10 receptor (IL-10R) and of soluble HLA-G (sHLA-G) and IL-10 by polyp epithelial cells. …”
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Distribution and species composition of potato viruses in the Novosibirsk region by V. S. Maslennikova, M. B. Pykhtina, K. A. Tabanyukhov, E. V. Shelikhova, K. I. Mosalev, A. V. Katokhin, A. A. Bondar, A. B. Beklemishev, M. I. Voevoda

“…The obtained results of the strain affiliation of NR samples lay the basis for the development of DNA and immunodiagnostic systems for identifying PVY circulating in NR, as well as for elucidating the source and routes of entry of specific virus strains.…”
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Impaired Iron–Sulfur Cluster Synthesis Induces Mitochondrial PARthanatos in Diabetic Cardiomyopathy by Mengyi Wang, Shiwu Zhang, Jinwei Tian, Fan Yang, He Chen, Shuzhi Bai, Jiaxin Kang, Kemiao Pang, Jiayi Huang, Mingjie Dong, Shiyun Dong, Zhen Tian, Shaohong Fang, Huitao Fan, Fanghao Lu, Bo Yu, Shuijie Li, Weihua Zhang

“…Abstract Diabetic cardiomyopathy (DCM), a severe complication of diabetes, is characterized by mitochondrial dysfunction, oxidative stress, and DNA damage. Despite its severity, the intrinsic factors governing cardiomyocyte damage in DCM remain unclear. …”
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Shrub encroachment alters the diversity of soil fungal communities in topsoil, but not in subsoil under alpine grassland of the Qinghai-Tibetan plateau by Wenming Ma, Tenzing Droma, Changting Wang, Xiangli Ma

“…We investigated soil fungal communities in top- and subsoils of shrub-encroached patches (Potentilla fruticosa, Spiraea alpina and Caragana microphylla) and the open grassland using nuclear ribosomal DNA internal transcribed spacer (ITS). A total of 48 samples were collected. …”
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The Fungi-specific histone Acetyltransferase Rtt109 mediates morphogenesis, Aflatoxin synthesis and pathogenicity in Aspergillus flavus by acetylating H3K9 by Ruilin Sun, Meifang Wen, Lianghuan Wu, Huahui Lan, Jun Yuan, Shihua Wang

“…The amount of aflatoxins synthesized by △rtt109 in the PDB liquid medium was significantly decreased We also found that the △rtt109 strain was extremely sensitive to DNA damage stress. Through the maize seed infection experiment, we found that the growth of △rtt109 on the surface of affected corn was largely reduced, and the amount of aerial mycelium decreased significantly, which was consistent with the results on the artificial medium. …”
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Biogenic synthesis of titanium nanoparticles by Streptomyces rubrolavendulae for sustainable management of Icerya aegyptiaca (Douglas) by Enayat M. Elqady, Eman El-said, Asmaa A. Tharwat, Lina A. Abou El-Khashab, Inas M. Y. Mostafa, Fatma Z. Hamed, Wesam M. Morsi, Mohamed M. Rezk, Inas M. Abou El-Enain

“…In all, seven actinobacterial isolates, the most potent of which has the potential to produce titanium hydroxide-based nanoparticles (TiO2-NP2), have DNA sequences that are 99.9% like those of Streptomyces rubrolavendulae (MCN2) according to nucleotide alignment and a phylogenetic tree. …”
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Multiple low-dose radiation ameliorates type-2 diabetes mellitus via gut microbiota modulation to activate TLR4/MyD88/NF-κB pathway by Lijing Qin, Rongrong Liu, Zhen Jia, Weiqiang Xu, Li Wang, Hongyuan Tian, Xinru Lian, Wen Li, Yali Qi, Huan He, Zhicheng Wang

“…The changes of blood glucose levels, body weight, and the damage of pancreas were measured. In addition, 16 S rDNA amplicon sequencing was used to detect gut microbiota alteration. …”
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The Gut Microbiota and Its Relevance to Peripheral Lymphocyte Subpopulations and Cytokines in Patients with Rheumatoid Arthritis by Yuan Li, Sheng-Xiao Zhang, Xu-Fang Yin, Ming-Xing Zhang, Jun Qiao, Xiao-Hong Xin, Min-Jing Chang, Chong Gao, Ya-Feng Li, Xiao-Feng Li

“…Fecal samples from 205 RA patients and 199 healthy controls (HCs) were collected for bacterial DNA extraction and 16S ribosomal RNA (rRNA) gene sequencing. …”
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REDD1 mediates HDM-induced nuclear-cytoplasmic translocation and release of IL-33 in airway epithelial cells by downregulating Nrf2 by Tian Luo, Wentao Ji, Yuxin Gong, Lichang Chen, Chao Liu, Dandan Zhang, Xi Li, Yanhua Lv

“…Abstract Objective This study aims to investigate whether REDD1 (Regulated in Development and DNA Damage Responses 1) mediates the nuclear-to-cytoplasmic translocation and release of IL-33 in airway epithelial cells induced by house dust mites (HDM). …”
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Targeted sequencing analysis pipeline for species identification of human pathogenic fungi using long-read nanopore sequencing by Nattapong Langsiri, Navaporn Worasilchai, Laszlo Irinyi, Piroon Jenjaroenpun, Thidathip Wongsurawat, Janet Jennifer Luangsa-ard, Wieland Meyer, Ariya Chindamporn

“…Abstract Among molecular-based techniques for fungal identification, Sanger sequencing of the primary universal fungal DNA barcode, the internal transcribed spacer (ITS) region (ITS1, 5.8S, ITS2), is commonly used in clinical routine laboratories due to its simplicity, universality, efficacy, and affordability for fungal species identification. …”
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Transcriptomic analysis of Andrias davidianus meat and experimental validation for exploring its bioactive components as functional foods by Changge Guan, Zhenglin Tan, Shucheng Li, Yi Wang, Naoyuki Yamamoto, Chong Zhang, Songjun Wang, Junjie Chen, Xin-Hui Xing

“…In this study, we illustrated full-length transcriptome sequencing to interpret the proteome of CGS meat and obtain 10703 coding DNA sequences. By functional annotation and amino acid composition analysis, we have discovered various genes related to signal transduction, and 16 genes related to longevity. …”
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Resveratrol enhances post-injury muscle regeneration by regulating antioxidant and mitochondrial biogenesis by Xiaoli Qin, Wenjing Niu, Kai Zhao, Yawen Luo, Wenfang Wang, Yang He, Fuyu Yang, Binghai Cao, Min Du, Huawei Su

“…Transcriptomic analysis identified enriched pathways related to muscle regeneration and mitochondrial biogenesis, with increased expression of mitochondrial transcription factors and higher mitochondrial DNA content in RES-treated mice. Furthermore, RES enhanced antioxidant capacity via the Kelch-like ECH-associated protein 1 (KEAP-1)/nuclear factor erythroid 2-related factor 2 (NRF2)/heme oxygenase-1 (HO-1) signaling pathway, as indicated by elevated activities of total antioxidant capacity, Glutathione peroxidase (GSH-PX), and superoxidase dismutase (SOD). …”
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Evaluating the Association Between Methylenetetrahydrofolate Reductase (Rs1801131 and Rs1801133) Gene Polymorphisms and Severity of Coronary Lesions in Patients With STEMI and NSTE... by Behnam Nazarzadeh, Saeedeh sadat Ghazanfari, Farzaneh Karimi, Seyed ali Moezibady, Fatemeh Salmani, Kazem Dastjerdi, Hamidreza Mohammadi

“…The angiographical characteristics of CAD were defined by the SYNTAX score. Genomic DNA was isolated from peripheral blood and genotyped for rs1801131 and rs1801133 using the TaqMan real‐time PCR method. …”
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