Inhibition of Endothelial Cell Tube Formation by Anti-Vascular Endothelial Growth Factor/Anti-Angiopoietin-2 RNA Nanoparticles by Cheng Zhong, Zhanquan Shi, Chia-Yang Liu, Daniel W. Binzel, Kai Jin, Xin Li, Peixuan Guo, S. Kevin Li

“…RNA nanoparticles, derived from the packaging RNA three-way junction motif (pRNA-3WJ) of the bacteriophage phi29 DNA packaging motor, have been demonstrated to be thermodynamically and chemically stable, with promise as a nanodelivery system. …”
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Cell-free fat extract improves embryo development and clinical outcomes in older women with previous in-vitro fertilization failure by Xiaowei Zhou, Hanfei Zhu, Jingru Duan, Lan Xia, Shen Zhao, Huihui Xu, Xian Wu, Xiaoling Wang, Bufang Xu, Yi Cao, Aijun Zhang

“…In addition, we performed immunofluorescence staining, multiplex immunoassay, whole-genome amplification and DNA sequencing, time-lapse embryo monitoring, and in vitro experiments. …”
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Biofabrication of copper oxide nanoparticles using Dalbergia sisso leaf extract for antibacterial, antibiofilm and antioxidant activities by Muhammad Hussnain Siddique, Maimona Sadia, Saima Muzammil, Muhammad Saqalein, Asma Ashraf, Sumreen Hayat, Saba Saba, Arif Muhammad Khan, Abeer Hashem, Graciela Dolores Avila-Qezada, Elsayed Fathi Abd-Allah

“…The effect of different concentrations of nanoparticles on cell membrane disruption was also investigated and a significant increase (p < 0.05) in the leakage of cellular content such as DNA, proteins and reducing sugar was measured. These nanoparticles also showed antibiofilm potential and a significant increase (p < 0.05) in biofilm inhibition was observed by increasing the concentration of nanoparticles. …”
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Exploring the Pharmacokinetics and Gut Microbiota Modulation of Hesperidin and Nobiletin from Mandarin Orange Peel in Experimental Dogs: A Pilot Study by Jun Nakahigashi, Makoto Kurikami, Satomi Iwai, Sadahiko Iwamoto, Shou Kobayashi, Eiji Kobayashi

“…Analysis included microbiota profiling via 16S rDNA sequencing and observational assessment of cognitive indicators in a pilot study involving senior dogs with Alzheimer’s disease. …”
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Application of cold atmospheric plasma for decontamination of toxigenic fungi and mycotoxins: a systematic review by Amanda Cristina Dias de Oliveira, Sher Ali, Carlos Humberto Corassin, Sana Ullah, Karina Nascimento Pereira, James Leon Walsh, Nataša Hojnik, Carlos Augusto Fernandes de Oliveira

“…The effectiveness of cold plasma depends on operational parameters and the specific characteristics of the treated food, with notable efficacy in degrading aflatoxin B1 and deoxynivalenol by converting them into less toxic substances and inhibiting their spores and DNA responsible for their biosynthesis. While the data demonstrates that cold atmospheric plasma has minimal impact on food composition, further research is needed to fully assess the nature of the degradation products of mycotoxins, its influence on food quality attributes and to optimize application strategies for different products.…”
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Chemokine Receptor 2 (CXCR2) Gene Variants and Their Association with Periodontal Bacteria in Patients with Chronic Periodontitis by Denisa Kavrikova, Petra Borilova Linhartova, Svetlana Lucanova, Hana Poskerova, Antonin Fassmann, Lydie Izakovicova Holla

“…A total of 500 unrelated subjects participated in this case-control study. 329 CP patients and 171 healthy nonperiodontitis controls were analyzed using polymerase chain reaction techniques for three single-nucleotide polymorphisms (SNPs): +785C/T (rs2230054), +1208T/C (rs1126579), and +1440A/G (rs1126580). A DNA microarray detection kit was used for the investigation of the subgingival bacterial colonization, in a subgroup of CP subjects (N=162). …”
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Gene expression in gastric biopsies from patients with Helicobacter pylori infection by D. Davoudi Oskouei, H. Akpınar, S. Sarıoğlu, P. Keskinoğlu, M. Soytürk, İ. Şimşek, Ö. Yılmaz

“…Total RNA was extracted from antral and corpus biopsies and cDNA was synthesized. Results: Forty-eight patients were positive for H. pylori infection based on both RUT and histopathology or culture positivity. …”
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重组pDC316-MCMV／hNIS真核表达质粒的构建及在肿瘤细胞的功能 by 周泉波, 陈汝福, 李志花, 周嘉嘉, 唐启彬, 陈积圣

“…【结果】序列分析结果证实克隆片段与文献报道的hNIS基因eDNA序列完全一致。实验组转染后48h，在CAPAN-Ⅱ细胞、PANC-1细胞及SK-OV-3细胞内均能检测到hNISmRNA高水平表达，对照组基本无hNISmRNA表达。…”
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PWWP domain-containing protein Crf4-3 specifically modulates fungal azole susceptibility by regulating sterol C-14 demethylase ERG11 by Pengju Yu, Shuting Ye, Mi Zhou, Long Zhang, Zhongchi Zhang, Xianyun Sun, Shaojie Li, Chengcheng Hu

“…In this study, leveraging the identified key promoter region of erg11 that controls its response to azoles in Neurospora crassa, we pinpointed a protein, Crf4-3, which harbors a PWWP domain and exerts a positive regulatory influence on azole resistance, as determined by DNA pulldown assays. The removal of Crf4-3 results in heightened sensitivity to azoles while remaining unaffected by other stressors tested. …”
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Low dose ATG-Fresenius for GVHD prophylaxis: a comparative study with ATG-Thymoglobulin by Itai Falicovich, Boaz Nachmias, Shlomo Elias, Eran Zimran, Adir Shaulov, Polina Stepensky, Batia Avni, Sigal Grisariu

“…Bacteremia and CMV reactivation rates were similar, but EBV DNA viremia was higher in the ATG-T group (22% vs. 8%, p=0.047), with one case of Post-Transplant Lymphoproliferative Disorder (PTLD) in the ATG-T group. …”
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A genogeographic study of the Kyrgyz mountain merino via microsatellite markers by A. В. Bekturov, Zh. T. Isakova, V. N. Kipen, T. Dzh. Chortonbaev, S. B. Mukeeva, S. K. Osmonaliev, К. A. Aitbaev

“…The aim was to ascertain the genetic and geographical structure of the Kyrgyz mountain merino (KMM). We analyzed DNA samples of 109 Kyrgyz mountain merino specimens, bred in three state breeding factories (STB), including“Orgochor” in the Issykul Province,“Katta-Taldyk” in the Osh Province and STb named after Luschikhin in the Talas Province. …”
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Expression Profile of Genes Potentially Associated with Adequate Glycemic Control in Patients with Type 2 Diabetes Mellitus by Sâmia Cruz Tfaile Corbi, Alliny Souza Bastos, Rafael Nepomuceno, Thamiris Cirelli, Raquel Alves dos Santos, Catarina Satie Takahashi, Cristiane S. Rocha, Silvana Regina Perez Orrico, Claudia V. Maurer-Morelli, Raquel Mantuaneli Scarel-Caminaga

“…Moreover, genes related to lipid metabolisms and DNA replication/repair were influenced by the glycemic control. …”
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Detection of I491F and V170F rpoB mutations associated with misdiagnosis of rifampicin resistance among patients with drug-susceptible tuberculosis treatment failure, Myanmar, 2022 by Phyu Win Ei, Mi Mi Htwe, Myat Htut Nyunt, Aye Su Mon, Zaw Myint, Wint Wint Nyunt, Su Mon Win, Sandar Aung, Wai Myat Thwe, Wah Wah Aung

“…Results: A total of 32 culture-positive isolates with DNA qualified for genome sequencing were included in the study. …”
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Dual role of Cathepsin S in cutaneous melanoma: insights from mendelian randomization and bioinformatics analysis by Linsa Zhou, Qiang Zhou, Qian Guo, Peng Lai, Chen Rui, Wanqing Li, Xuemei Chen, Yue Zhuo, Xiaoping Zhong, Sen Lin

“…Abstract Background Cutaneous melanoma (CM) is strongly associated with ultraviolet (UV) radiation, which contributes to the transformation of melanocytes into melanoma by inducing specific DNA damage. Here, we investigated the causal relationship between CM and genes related to sun-damaged skin, exploring specific target genes through various bioinformatics analyses. …”
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Isolation and identification of the causal agent of gummy stem blight disease in Cucumis sativus caused by a bacterial pathogen in China by Yidan Wang, Mengjun Jin, Chengde Yang, Fengfeng Cai, Richard Osei, Ting Ma, Cuiwen Zhang, Na Qi

“…Meanwhile, its internal transcribed spacer sequence (ITS) and DNA gyrase A subunit (gyrA) were both more than 99% homologous and clustered on the same branch with B. subtilis. …”
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Impact of age-related changes in buccal epithelial cells on pediatric epigenetic biomarker research by Sarah M. Merrill, Chaini Konwar, Fizza Fatima, Kristy Dever, Julia L. MacIsaac, Nicole Letourneau, Gerald F. Giesbrecht, Deborah Dewey, Gillian England-Mason, Candace R. Lewis, Dennis Wang, Ai Ling Teh, Michael J. Meaney, Andrea Gonzalez, Jennie G. Noll, Carolina De Weerth, Nicole R. Bush, Kieran J. O’Donnell, S. Evelyn Stewart, Michael S. Kobor

“…Abstract Cheek swabs, heterogeneous samples consisting primarily of buccal epithelial cells, are widely used in pediatric DNA methylation studies and biomarker creation. …”
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miR-1233-3p Inhibits Angiopoietin-1-Induced Endothelial Cell Survival, Migration, and Differentiation by Veronica Sanchez, Sharon Harel, Anas Khalid Sa’ub, Dominique Mayaki, Sabah N. A. Hussain

“…Biotinylated miRNA pull-down assays showed that p53 and DNA damage-regulated 1 (PDRG1) is a direct target of miR-1233-3p in HUVECs. …”
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Massive subcutaneous filariosis by Filaria martis in beech marten (Martes foina) in Italy by Giovanni Sgroi, Ranju Ravindran Santhakumar Manoj, Ettore Napoli, Nicola D'Alessio, Maria Gabriella Lucibelli, Claudio de Martinis, Esterina De Carlo, Peyman Khademi, Alireza Sazmand, Vincenzo Veneziano

“…Haemolymph, gut, and salivary glands of the Ixodes ricinus ticks collected from the infected marten scored negative for F. martis larvae and DNA by dissection and PCR, respectively. This study reported for the first time F. martis subcutaneous filariosis in southwestern Italy, previously outlined only in northern and southeastern areas, indicating the circulation of this poorly investigated filarioid. …”
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Whole exome sequencing-based homologous recombination deficiency test for epithelial ovarian cancer by Ying-Cheng Chiang, Hsien-Neng Huang, Kuan-Ting Kuo, Wuh-Liang Hwu, Po-Han Lin

“…Nine patients had mutations in the genes involved in HR DNA repair, and all of them were positive for HRD. …”
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Codon usage patterns and genomic variation analysis of chloroplast genomes provides new insights into the evolution of Aroideae by Xinbi Jia, Jiaqi Wei, Yuewen Chen, Chenghong Zeng, Chan Deng, Pengchen Zeng, Yufei Tang, Qinghong Zhou, Yingjin Huang, Qianglong Zhu

“…Eight highly divergent regions (Pi > 0.064) were identified (ndhF, rpl32, ccsA, ndhE, ndhG, ndhF-rpl32, ccsA-ndhD, and ndhE-ndhG) , in which ndhE have the potential to serve as a reliable DNA marker to discriminate chloroplasts in Aroideae subfamily. …”
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