Femtomolar hydrogen sulfide detection via hybrid small-molecule nano-arrays by Xing Xing, Luyan Wu, Yuchen Zhang, Jiahao Pan, Yusuke Ishigaki, Huaqing Xie, Takanori Suzuki, Deju Ye, Jianhua Zhang, Weihua Zhang, Zhenda Lu

“…On-chip immunoassays, particularly nanoarray-based interfacial detection, offer promising avenues for ultra-sensitive analysis due to their confined reaction volumes and precise signal localization. Beyond the DNA or protein biomolecules array, this work presents a promising hybrid small molecule nano-array for H2S detection, using the power of dual molecules: a dye for fluorescence emission and a quencher with specific H2S reactivity. …”
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The impact of glucocorticoid receptor transactivation on context-dependent cell migration dynamics by Szonja Polett Pósa, Éva Saskői, Lili Bársony, Lőrinc Pongor, Fanni Fekete, János Papp, Anikó Bozsik, Attila Patócs, Henriett Butz

“…RNA sequencing revealed dex’s impact on cell adhesion and extracellular matrix signalling in TN cells and on DNA replication in ER + cells. Based on data from 1085 human breast cancer specimens, GR pathway expression correlated with migratory, extracellular matrix, and angiogenesis gene signatures. …”
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Occult hepatitis B virus infection: risk for a blood supply, but how about individuals’ health? by Michael X. Fu, Ahmed Elsharkawy, Brendan Healy, Celia Jackson, Daniel Bradshaw, Emma Watkins, Ines Ushiro-Lumb, Jaisi Griffiths, James Neuberger, Kathryn Maguire, Monica Desai, Neil McDougall, Nicole Priddee, Stephen T. Barclay, Stuart Blackmore, Peter Simmonds, William L. Irving, Heli Harvala

“…Summary: The implementation of effective blood donation screening for hepatitis B virus (HBV) anti-core antibodies with highly sensitive molecular HBV DNA detection in low-endemic countries like the United Kingdom has improved blood safety. …”
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Identification of microsatellite loci according to BAC sequencing data and their physical mapping to the bread wheat 5B chromosome by M. A. Nesterov, D. A. Afonnikov, E. M. Sergeeva, L. A. Miroshnichenko, M. K. Bragina, A. O. Bragin, G. V. Vasiliev, E. A. Salina

“…An algorithm utilizing the properties of complexity decompositions in  he sliding-window mode was used to detect DNA sequences with a repeat unit of 2–4 bp. Analysis of 17 770 contigs with the total length of 25 879 921 bp allowed for designing 113, 79, and 67 microsatellite (SSR) loci with a repeat unit of 2, 3, and 4 bp, respectively. …”
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Diagnostic performance of the DISQVER metagenomic sequencing tool for the identification of pathogens in febrile neutropenic patients: the ADNEMIA trial by Geneviève Héry-Arnaud, Christophe Burucoa, Marie Kempf, David Boutoille, Yohann Foucher, Philippe Lanotte, Maxime Pichon, Stéphane Corvec, Cécile Le Brun, Helene Pailhories

“…Sequencing technologies have led to the development of diagnostic approaches based on the detection of circulating DNA in blood. This study will aim to assess the clinical utility of metagenomic next-generation sequencing (mNGS)-DISQVER technology in detecting pathogenic microorganisms from blood samples of patients undergoing high-risk FN treatment.Methods and analysis This nationwide, prospective, multicentre, interventional, proof-of-concept clinical trial will enrol 200 patients. …”
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Antimicrobial resistance and epidemiological patterns of Streptococcus pyogenes in Türkiye by Nihan Ünübol, Nese Caglayan, Sinan Cebeci, Yeşim Beşli, Banu Sancak, Neval Yurttutan Uyar, Siavosh Salmanzadeh Ahrabi, Masoud Alebouyeh, Tanıl Kocagöz

“…Methods: We determined the minimum inhibitory concentration using the penicillin gradient test and performed emm typing and DNA fingerprinting via pulsed-field gel electrophoresis (PFGE) to analyze the clonal spread of 92 S. pyogenes strains isolated from two hospitals in Türkiye between 2020 and 2022. …”
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Optimization of a Loop-Mediated Isothermal Amplification Assay as a Point-of-Care Tool for the Detection of Wuchereria bancrofti in Human Blood in Tana River Delta, Kenya by Kinyatta Nancy, Wambua Lillian, Mutahi Wilkinson, Mugasa Claire, Kamau Luna, Wachira Dorcas, Githae Rosemary, Lusweti Japheth, Ichugu Christine, Waigi Emily, Kagai Jim

“…In this study, we evaluated loop-mediated isothermal amplification (LAMP) tests to amplify Wuchereria bancrofti DNA in patients’ blood. The amplicons were tested by both pH-sensitive dyes for enhanced visual detection and agarose gel electrophoresis. …”
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Combination of ataxia telangiectasia and Rad3-related inhibition with ablative radiotherapy remodels the tumor microenvironment and enhances immunotherapy response in lung cancer by Jenny Ling-Yu Chen, Chun-Kai Pan, Li-Cheng Lin, Yu-Sen Huang, Tsung-Hsuan Huang, Shu-Jyuan Yang, Sung-Hsin Kuo, Yu-Li Lin

“…ATR inhibitor pretreatment decreased cell survival after irradiation by inhibiting DNA double-strand break repair, inducing mitotic cell death, and altering cell cycle progression. …”
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Effective cancer immunotherapy combining mRNA-encoded bispecific antibodies that induce polyclonal T cell engagement and PD-L1-dependent 4-1BB costimulation by Oana Hangiu, Oana Hangiu, Oana Hangiu, Rocío Navarro, Susana Frago, Laura Rubio-Pérez, Laura Rubio-Pérez, Laura Rubio-Pérez, Antonio Tapia-Galisteo, Antonio Tapia-Galisteo, Antonio Tapia-Galisteo, Laura Díez-Alonso, Laura Díez-Alonso, Laura Díez-Alonso, Marina Gómez-Rosel, Marina Gómez-Rosel, Marina Gómez-Rosel, Noelia Silva-Pilipich, Noelia Silva-Pilipich, Lucía Vanrell, Cristian Smerdou, Cristian Smerdou, Kenneth A. Howard, Laura Sanz, Luis Álvarez-Vallina, Luis Álvarez-Vallina, Luis Álvarez-Vallina, Marta Compte

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Pollen metabarcoding reveals a broad diversity of plant sources available to farmland flower visitors near tropical montane forest by B. Karina Montero, B. Karina Montero, Nicole Gamboa-Barrantes, Nicole Gamboa-Barrantes, Geovanna Rojas-Malavasi, Geovanna Rojas-Malavasi, E. Jacob Cristóbal-Perez, E. Jacob Cristóbal-Perez, Gilbert Barrantes, Gilbert Barrantes, Gilbert Barrantes, Alfredo Cascante-Marín, Alfredo Cascante-Marín, Alfredo Cascante-Marín, Paul Hanson, Paul Hanson, Manuel A. Zumbado, Ruth Madrigal-Brenes, Ruth Madrigal-Brenes, Ruth Madrigal-Brenes, Silvana Martén-Rodríguez, Silvana Martén-Rodríguez, Silvana Martén-Rodríguez, Mauricio Quesada, Mauricio Quesada, Eric J. Fuchs, Eric J. Fuchs, Eric J. Fuchs, Eric J. Fuchs

“…Here, we conducted monthly surveys over a period of one year to study the diversity of insect visitors in dominant fruit crops—avocado, plum, apple, and blackberry—and used pollen DNA metabarcoding to characterize the community of plant sources in and around low-intensive farmland bordered by protected montane forest in Costa Rica. …”
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Clinical Characteristics of Aldosterone- and Cortisol-Coproducing Adrenal Adenoma in Primary Aldosteronism by Lu Tang, Xintao Li, Baojun Wang, Xin Ma, Hongzhao Li, Yu Gao, Liangyou Gu, Wenyuan Nie, Xu Zhang

“…Seventeen cases with KCNJ5 mutations were detected among the 22 A/CPA DNA samples, but no PRKACA or other causative mutations were observed. …”
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Meta-analysis of the association between overexpression of RAD51 family genes and prognosis and clinical features in breast cancer by Yong-Cheng Liu, Jun Shen

“…Abstract The RAD51 family of genes play a crucial role in the repair of DNA damage, and increasing evidence suggests a close link between the aberrant expression of RAD51 family genes and the development of breast cancer. …”
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The <i>Meq</i> Genes of Nigerian Marek’s Disease Virus (MDV) Field Isolates Contain Mutations Common to Both European and US High Virulence Strains by Joseph N. Patria, Luka Jwander, Ifeoma Mbachu, Levi Parcells, Brian Ladman, Jakob Trimpert, Benedikt B. Kaufer, Phaedra Tavlarides-Hontz, Mark S. Parcells

“…Materials and Methods: DNA was isolated from FTA cards obtained at 12 farms affected by increased MD in the Plateau State, Nigeria. …”
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Development of the genetic classification of Aegilops columnaris Zhuk. chromosomes based on the analysis of introgression lines Triticum aestivum×Ae. columnaris by A. A. Shishkina, A. Yu. Dragovich, A. S. Rouban, S. N. Sibikeev, A. E. Druzhin, E. D. Badaeva

“…Taking into consideration our previous results of FISH analyses, three other Ae. columnaris chromosomes can be assigned to homoeologous groups 1, 5 and 7 of the U-genome based on the location of 5S and 45S rDNA loci (1U and 5U) or pSc119.2 probe distribution (7U). …”
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Unveiling early-life microbial colonization profile through characterizing low-biomass maternal-infant microbiomes by 2bRAD-M by Shuwen Hou, Yuesong Jiang, Feng Zhang, Tianfan Cheng, Dan Zhao, Jilong Yao, Ping Wen, Ping Wen, Lijian Jin, Shi Huang

“…However, analyzing these samples often faces technical challenges and limitations of low-resolution using conventional approaches due to their low microbial biomass.MethodsHere, we employed the type IIB restriction enzymes site-associated DNA sequencing for microbiome (2bRAD-M) as a reduced metagenomics method to address these issues and profile species-level microbial composition. …”
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Characterization of Myf6 and association with growth traits in swimming crab (Portunus trituberculatus) by Baohua Duan, Weibiao Liu, Chen Zhang, Tongxu Kang, Haifu Wan, Shumei Mu, Yueqiang Guan, Zejian Li, Yang Tian, Yuqin Ren, Xianjiang Kang

“…Results In the current study, we characterized the Myf6 gene for Portunus trituberculatus to better understand its biological function. The full-length cDNA of Myf6 was 4,101 bp, with a 915 bp open reading frame encoding 304 amino acids. …”
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Discovering CRISPR-Cas system with self-processing pre-crRNA capability by foundation models by Wenhui Li, Xianyue Jiang, Wuke Wang, Liya Hou, Runze Cai, Yongqian Li, Qiuxi Gu, Qinchang Chen, Peixiang Ma, Jin Tang, Menghao Guo, Guohui Chuai, Xingxu Huang, Jun Zhang, Qi Liu

“…Notably, one homolog, EphcCasλ, is experimentally validated for self-processing pre-crRNA, DNA cleavage, and trans-cleavage, showing promise for CRISPR-based pathogen detection. …”
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Microbiome of <i>Hyalomma dromedarii</i> (Ixodida: Ixodidae) Ticks: Variation in Community Structure with Regard to Sex and Host Habitat by Nighat Perveen, Alejandro Cabezas-Cruz, Daniil Iliashevich, Lianet Abuin-Denis, Olivier Andre Sparagano, Arve Lee Willingham

“…A total of 40 ticks (male (15), female (15), and nymph (10)) were selected from tick samples collected from camels and processed for genomic DNA and next-generation sequencing using the Illumina MiSeq platform. …”
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c-FLIP and microRNA 708-5p gene expression in newly diagnosed and chemotherapy in acute myeloid leukemia of Iraqi patient by Jaafar Sadiq Jaafar, Hiba Muneer Abdual Hassan Al-Kafagi, Israa Hussein Hamzah

“…The methods employed in the analysis include total RNA extraction, complementary cDNA synthesis, and quantitative real-time polymerase chain reaction (PCR) were used to evaluate the gene expression of cFLIP and miRNA-708-5p. …”
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Two-Step Transfer Learning Improves Deep Learning–Based Drug Response Prediction in Small Datasets: A Case Study of Glioblastoma by Jie Ju, Ioannis Ntafoulis, Michelle Klein, Marcel JT Reinders, Martine Lamfers, Andrew P Stubbs, Yunlei Li

“…The GBM is aggressive, and most patients do not benefit from the only approved chemotherapeutic agent TMZ. O6-methylguanine-DNA methyltransferase (MGMT) promoter methylation status is the only biomarker for TMZ responsiveness but has shown limited predictive power. …”
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