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4901
Decoding the chicken gastrointestinal microbiome
Published 2025-01-01“…Specifically, bacterial 16S ribosomal DNA sequences from GIT samples associated with various breeds, differing in age, GIT sites (caecum, faeces, ileum and jejunum) and geographical location were obtained from the Sequence Read Archive and analysed using the MGnify pipeline. …”
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4902
Genetic diversity of potato varieties bred in Russia and near-abroad countries based on polymorphism of SSR-loci and markers associated with resistance R-genes
Published 2016-12-01“…Additionally, we have studied these varieties for the distribution of 8 DNA markers associated with three R-genes involved in the control of resistance to two quarantine objects: the potato wart Synchytrium endobioticum and golden potato cyst nematode Globodera rostochiensis, which occur locally in some regions of the Russian Federation. …”
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4903
Analysis of Nucleotide Variations in Genes of Iron Management in Patients of Parkinson's Disease and Other Movement Disorders
Published 2011-01-01“…In the brain, an accumulation of iron or an increase in its availability has been associated with the development and/or progression of different degenerative processes, including Parkinson's disease, while iron paucity seems to be associated with cognitive deficits, motor dysfunction, and restless legs syndrome. In the search of DNA sequence variations affecting the individual predisposition to develop movement disorders, we scanned by DHPLC the exons and intronic boundary regions of ceruloplasmin, iron regulatory protein 2, hemopexin, hepcidin and hemojuvelin genes in cohorts of subjects affected by Parkinson's disease and idiopathic neurodegeneration with brain iron accumulation (NBIA). …”
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4904
Knowledge and attitude among Egyptian medical students regarding the role of human papillomavirus vaccine in prevention of oropharyngeal cancer: a questionnaire-based observational...
Published 2025-01-01“…Abstract Squamous cell carcinomas in several anatomical sites are caused by human papillomaviruses (HPV), and oncogenic double-stranded DNA viruses. There are about 200 genotypes; HPV16 is the most often occurring variant. …”
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4905
Hypoxia-induced CTCF mediates alternative splicing via coupling chromatin looping and RNA Pol II pause to promote EMT in breast cancer
Published 2025-02-01“…Notably, CTCF mediates COL5A1 promoter-alternatively spliced exon upstream looping that regulates DNA demethylation at distal exon64A. This further regulates the CTCF-mediated RNA polymerase II pause at COL5A1exon64A, leading to its inclusion in promoting the EMT under hypoxia. …”
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4906
Case Study of Somaclonal Variation in Resistance Genes Mlo and Pme3 in Flaxseed (Linum usitatissimum L.) Induced by Nanoparticles
Published 2017-01-01“…In this paper, it was essential to obtain DNA material from all tissue culture development stages from one donor plant to record changes in each nucleotide sequence. …”
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4907
中国南方三个Hunter综合征家系的病因学研究
Published 2023-05-01“…方法在临床初诊和系谱分析的基础上,首先进行尿糖胺聚糖定性检测,然后抽取患儿及其亲属的抗凝血,提取DNA并通过PCR、Sanger测序对<italic>IDS</italic>基因进行序列分析。…”
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4908
Low-Dose Mitomycin C Decreases the Postoperative Recurrence Rate of Pterygium by Perturbing NLRP3 Inflammatory Signalling Pathway and Suppressing the Expression of Inflammatory Fac...
Published 2019-01-01“…Mitomycin C (MMC) is an antineoplastic antibiotic that inhibits DNA, RNA, and protein synthesis. In recent years, although MMC has proven useful for the treatment of pterygium, its application has been controversial because of its clear toxicity and the possibility of ocular complications. …”
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4909
Butyric Acid Modulates Gut Microbiota to Alleviate Inflammation and Secondary Bone Loss in Ankylosing Spondylitis
Published 2024-12-01“…Various techniques, including an enzyme-linked immunosorbent assay (ELISA), magnetic resonance imaging (MRI), micro-CT, histology, quantitative PCR (qPCR) for intestinal tight junction protein expression, and 16S rDNA sequencing to analyze gut microbiota abundance, were employed to assess the inflammation and bone health in the target tissues. …”
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4910
TẠO DÒNG VÀ BIỂU HIỆN GEN nahC MÃ HÓA 1,2-dihydroxynaphthalene dioxygenase CỦA VI KHUẨN Paenibacillus naphthalenovorans 4B1
Published 2024-09-01“…Sau khi xác nhận trình tự DNA chính xác, gen nahC được ghép nối vào vector biểu hiện pColdTM II và biến nạp vào vi khuẩn Escherichia coli BL21. …”
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4911
First Report on Microbial-Derived Polydeoxyribonucleotide: A Sustainable and Enhanced Alternative to Salmon-Based Polydeoxyribonucleotide
Published 2025-01-01“…Electrophoretic analysis revealed that L-PDRN consists of smaller DNA fragments (under 100 bp) compared to salmon-derived PDRN (200–800 bp), suggesting greater bioavailability and skin absorption. …”
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4912
TET2-mediated 5-hydroxymethylcytosine of TXNIP promotes cell cycle arrest in systemic anaplastic large cell lymphoma
Published 2025-01-01“…Abstract Background 5-Hydroxymethylcytosine (5hmC) modification represents a significant epigenetic modification within DNA, playing a pivotal role in a range of biological processes associated with various types of cancer. …”
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4913
Implications of cytokine genes polymorphisms in Jordanian patients with obsessive compulsive disorder
Published 2025-01-01“…The tumor necrosis factor-α (TNF-α) SNP (rs1800629) DNA was sequenced by the Sanger method. The obtained data were analyzed using the GraphPad Prism method. …”
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4914
姜酚肟对谷氨酸诱导PC12细胞损伤的保护作用
Published 2008-01-01“…【方法】体外培养PC12细胞,建立谷氨酸诱导PC12细胞损伤的模型;采用MTT法检测细胞活力,流式细胞术和Hoechst 33258 DNA 染色法检测细胞凋亡。【结果】经5 mmol/L的谷氨酸处理24 h后,PC12细胞活力比对照组降低,仅为对照组的58.3﹪。…”
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4915
Novel Antibacterial 4-Piperazinylquinoline Hybrid Derivatives Against <i>Staphylococcus aureus</i>: Design, Synthesis, and In Vitro and In Silico Insights
Published 2024-12-01“…Further in silico docking and molecular dynamics studies highlighted strong interactions of <b>5k</b> with bacterial enzymes, such as tyrosyl-tRNA synthetase, pyruvate kinase, and DNA gyrase B, suggesting potential modes of action. …”
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4916
Genomic insights into a multidrug-resistant pandoraea apista clinical isolate carrying blaOXA-153 from China
Published 2025-03-01“…In this study, we describe a strain of P. apista harboring the blaOXA-153, isolated from the bronchoalveolar lavage (BAL) fluid of an inpatient in China. Methods: The genomic DNA of P. apista strain PA167 was sequenced using the Illumina NovaSeq 6000 system and assembled with SPAdes v.3.13.0. …”
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4917
The Construction of Heterothallic Strains of <i>Komagataella kurtzmanii</i> Using the I-SceI Meganuclease
Published 2025-01-01“…The methylotrophic yeast <i>Komagataella kurtzmanii</i> belongs to the group of homothallic fungi that are able to spontaneously change their mating type by inversion of chromosomal DNA in the MAT locus region. As a result, natural and genetically engineered cultures of these yeasts typically contain a mixture of sexually dimorphic cells that are prone to self-diploidisation and spore formation accompanied by genetic rearrangements. …”
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4918
Antimicrobial resistance, virulence gene profiling, and genetic diversity of multidrug-resistant Pseudomonas aeruginosa isolates in Mazandaran, Iran
Published 2024-12-01“…The genetic diversity among the strains was evaluated using the random amplification of polymorphic DNA (RAPD) technique. Clustering was based on a Dice similarity coefficient of ≥ 85%. …”
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4919
Synthesis and Characterization of Paclitaxel-Loaded Silver Nanoparticles: Evaluation of Cytotoxic Effects and Antimicrobial Activity
Published 2024-01-01“…AgNPs-PTX was determined to increase apoptosis cells by inducing DNA fragmentation. To determine the antimicrobial activity, the MIC (minimum inhibitory concentration) test was performed using 8 different bacteria and 2 different fungi. …”
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4920
The first two mitochondrial genomes from Apiotrichum reveal mitochondrial evolution and different taxonomic assignment of Trichosporonales
Published 2023-03-01“…The mitogenomes of Apiotrichum gracile and A. gamsii comprised circular DNA molecules, with sizes of 34,648 bp and 38,096 bp, respectively. …”
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