Investigation of malaria vectors Anopheles in non-endemic areas of Thailand: in proximity to workplaces housing foreign migrant workers by Nurhadi Eko Firmansyah, Thaksaporn Thongseesuksai, Thidarut Boonmars, Porntip Laummaunwai

“…Subsequently, the collected female mosquitoes underwent morphological identification using Standard Thailand keys and polymerase chain reaction analysis with rDNA ITS2 primers. Results Morphological identification revealed that 201 (29.6%) belonged to the Barbirostris complex. …”
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First Detection and Genomic Characterization of Linezolid-Resistant <i>Enterococcus faecalis</i> Clinical Isolates in Bulgaria by Tanya V. Strateva, Preslava Hristova, Temenuga J. Stoeva, Hristina Hitkova, Slavil Peykov

“…Efs2503-bg was high-level resistant to linezolid (MIC > 256 mg/L) and displayed the G2576T mutation affecting three of the four 23S rDNA loci. Efs966-bg (MIC = 8 mg/L) carried a plasmid-located <i>optrA</i> determinant surrounded by <i>fexA</i> and <i>ermA</i>. …”
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Proximity‐dependent biotinylation reveals an interaction between ubiquitin‐specific peptidase 46 and centrosome‐related proteins by Kazuma Yoshioka, Reiko Nakagawa, Chi Lieu Kim Nguyen, Hayate Suzuki, Kiyohiro Ishigaki, Seiya Mizuno, Tsukasa Okiyoneda, Shizufumi Ebihara, Kazuya Murata

“…Protein ubiquitination extensively modulates protein functions and controls various biological processes, such as protein degradation, signal transduction, transcription, and DNA repair. Ubiquitination is a reversible post‐translational modification, and deubiquitinating enzymes cleave ubiquitin from proteins. …”
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First Molecular Identification of <i>Entamoeba</i> spp. in Sheep, Beef Cattle, and Dairy Cattle in Shanxi Province, North China by Ze-Xuan Wu, Han-Dan Xiao, Yuan-Hui He, Shi-Bo Huang, Jing Li, Yu Kang, Wen-Bin Zheng, Xing-Quan Zhu

“…To reveal the prevalence of <i>Entamoeba</i> in sheep and cattle in Shanxi Province, North China, fecal samples were collected from 311 sheep, 392 dairy cattle, and 393 beef cattle from three representative counties in the northern, central, and southern regions of Shanxi Province. DNA was extracted from the fecal samples and amplified by PCR with primers targeting the nuclear small subunit ribosomal RNA (SSU rRNA) gene of <i>Entamoeba</i> spp., followed by the sequencing of the positive products. …”
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Toxicity of antimony in housefly after whole-life-cycle exposure: Changes in growth, development, redox homeostasis, mitochondrial function, and fecundity by Yuming Zhang, Jinghua Lv, Xiaoli Sun, Yiwen Zhang, Menghua Shao, Bingbing Shi, Yajie Zhang, Jihai Gu, Chenchen Zhao, Fengsong Liu

“…Significantly, Sb demonstrated reproductive toxicity in the houseflies, as evidenced by reduced reproductive capacity, DNA damage, and ovarian abnormalities. The disturbance of hormonal synthesis and the MAPK pathway induced by Sb treatment may contribute to reproductive toxicity. …”
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p18 encoded by FgGMTV1 is responsible for asymptomatic infection in Fusarium graminearum by Lihang Zhang, Pengfei Li, Yanfei Wang, Shuangchao Wang, Lihua Guo

“…Our study introduces p18, a novel protein encoded by the DNA-C segment of the genomovirus FgGMTV1, which facilitates the transition from virus-induced hypovirulence to asymptomatic infection within Fusarium graminearum upon its expression. …”
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深圳地区育龄人群地中海贫血基因型分布调查

“…βCD71/72(+A ),共占94.71%?其中通过β珠蛋白基因全长DNA序列分析,首次在广西籍患者中发现1种在中国人种中罕见报道的突变:CD37突变(G→A),此前仅发现于中国广东人和阿富汗人中?…”
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Pivotal roles of Plasmodium falciparum lysophospholipid acyltransferase 1 in cell cycle progression and cytostome internalization by Junpei Fukumoto, Minako Yoshida, Suzumi M. Tokuoka, Eri Saki H. Hayakawa, Shinya Miyazaki, Takaya Sakura, Daniel Ken Inaoka, Kiyoshi Kita, Jiro Usukura, Hideo Shindou, Fuyuki Tokumasu

“…Transcriptomic analyses suggest that this deficiency impacts DNA replication and cell cycle regulation. Mass spectrometry-based enzyme assay and lipidomic analysis demonstrate that recombinant PfLPLAT1 exhibits lysophospholipid acyltransferase activity with a preference for unsaturated fatty acids as its acyl donors and lysophosphatidic acids as an acceptor, with its conditional knockout leading to abnormal lipid composition and marked morphological and developmental changes including stage arrest. …”
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融合蛋白TAT-RabGEF1原核表达载体的构建及蛋白表达 by 方虹仪, 邢纪斌, 罗晨芳

“…： 【目的】构建PET28a-TAT-RabGEF1重组质粒载体，在E.coli Rosetta（DE3）菌株中高效表达并纯化融合 蛋白，研究TAT-RabGEF1融合蛋白对肥大细胞的跨膜转导活性。【方法】以小鼠组织cDNA为模板，设计上游和下游 含有限制性酶切位点和TAT序列的引物，PCR扩增目的片段TAT-RabGEF1，并通过双酶切构建PET28a-TAT-Rab? …”
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Investigation of Insulin-Like Growth Factor 2 mRNA Binding Protein 2 Gene Polymorphisms in Type 2 Diabetes Patients by Duygu Yolal Ertural, Ümit Çınkır, Nurcan Aras

“…The aim of this study was to investigate in a Turkish population identified associations of IGF2BP2 variants rs4402960 and rs1470579 with T2D.Methods: We genotyped two SNPs of IGF2BP2 gene, rs1470579 and rs4402960 in 100 healthy individuals and 100 patients. DNA isolation was performed on peripheral blood samples from patients and healthy groups. …”
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Snapshots of Pseudomonas aeruginosa SOS response reveal structural requisites for LexA autoproteolysis by Filippo Vascon, Sofia De Felice, Matteo Gasparotto, Stefan T. Huber, Claudio Catalano, Monica Chinellato, Riccardo Mezzetti, Alessandro Grinzato, Francesco Filippini, Lorenzo Maso, Arjen J. Jakobi, Laura Cendron

“…Summary: Antimicrobial resistance poses a severe threat to human health and Pseudomonas aeruginosa stands out among the pathogens responsible for this emergency. The SOS response to DNA damage is crucial in bacterial evolution, influencing resistance development and adaptability in challenging environments, especially under antibiotic exposure. …”
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Betacoronaviruses Differentially Activate the Integrated Stress Response to Optimize Viral Replication in Lung-Derived Cell Lines by David M. Renner, Nicholas A. Parenti, Nicole Bracci, Susan R. Weiss

“…Interestingly, genetic ablation of growth arrest and DNA damage-inducible protein (GADD34) expression, an inducible protein phosphatase 1 (PP1)-interacting partner targeting eIF2α for dephosphorylation, did not significantly alter HCoV-OC43 or SARS-CoV-2 replication, while siRNA knockdown of the constitutive PP1 partner, constitutive repressor of eIF2α phosphorylation (CReP), dramatically reduced HCoV-OC43 replication. …”
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The Characteristic of Biofilm Formation in ESBL-Producing K. pneumoniae Isolates by Xiaofang Gao, Haili Wang, Zhijuan Wu, Pan Sun, Wei Yu, Donghua Chen, Yuhua Mao, Lili Fang, Jia Qian, Li Li, Qian Peng, Yanping Han

“…Based on the chromosomal DNA patterns we obtained, we identified 21 PFGE profiles from the 30 isolates, eight of which had high homology indicating that they may have genetic relationships and/or potential clonal advantages within the hospital. …”
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<i>Toxoplasma gondii</i> and Rabies—The Parasite, the Virus, or Both? by Ragan Wilson, Shannon Caseltine, Edith Will, Jeremiah Saliki, Ruth C. Scimeca

“…Due to the overlapping clinical signs of these two pathogens, the objective of this study was to evaluate the prevalence of <i>T. gondii</i> DNA in cerebellum tissue collected for rabies testing; cerebellum tissue from diverse animals is often submitted for this purpose. …”
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Screening of Indigenous Oxalate Degrading Lactic Acid Bacteria from Human Faeces and South Indian Fermented Foods: Assessment of Probiotic Potential by Sivasamy Gomathi, Ponnusamy Sasikumar, Kolandaswamy Anbazhagan, Sundaresan Sasikumar, Murugan Kavitha, M. S. Selvi, Govindan Sadasivam Selvam

“…These were identified as Lactobacillus fermentum and Lactobacillus salivarius using 16S rDNA sequencing. Three strains, Lactobacillus fermentum TY5, Lactobacillus fermentum AB1, and Lactobacillus salivarius AB11, exhibited good adhesion to HT-29 cells and strong antimicrobial activity. …”
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Hypoxia Enhances HIF1α Transcription Activity by Upregulating KDM4A and Mediating H3K9me3, Thus Inducing Ferroptosis Resistance in Cervical Cancer Cells by Jing Xiong, Meifang Nie, Chun Fu, Xiaoshan Chai, Yongjing Zhang, Ling He, Shujuan Sun

“…CC cells were co-transfected with shKDM4A or/and pcDNA3.1-HIF1α. Iron uptake and release were assessed using the isotopic tracer method. …”
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Pulmonary Bacterial Communities in Surgically Resected Noncystic Fibrosis Bronchiectasis Lungs Are Similar to Those in Cystic Fibrosis by Heather Maughan, Kristopher S. Cunningham, Pauline W. Wang, Yu Zhang, Marcelo Cypel, Cecilia Chaparro, D. Elizabeth Tullis, Thomas K. Waddell, Shaf Keshavjee, Mingyao Liu, David S. Guttman, David M. Hwang

“…Bacterial 16S rRNA gene libraries were constructed from lung tissue of 10 non-CF bronchiectasis and 21 CF patients, followed by DNA sequencing of isolates from each library. Community characteristics were analyzed and compared between the two groups. …”
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Molecular Mechanisms of Breast Cancer Drug Resistance and CRISPR/Cas9 Strategies to Overcome by Bashdar Mahmud Hussen, Bnar Saleh Ismael, Saman S. Abdulla, Noor Haval Jamal, Suhad Asad Mustafa, Zana Baqi Najmalddin, Mohammed Fatih Rasul

“…Recent research has effectively investigated and targeted particular genes linked to BC treatment resistance using CRISPR/Cas9 gene editing, including those linked to hormone receptor signaling, drug efflux transporters, and DNA repair pathways. The CRISPR/Cas9 technology's selective disruption or mutation of these genes provides valuable information about their role in resistance and paves the path for cutting-edge treatment options. …”
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Association Between GSK3β Gene Polymorphisms (rs334558 and rs3755557) with Schizophrenia Risk by Maryam Montazeri Dehbarez, Dormohammad Kordi Tamandani, Nasim Naeimi, Shahram Vaziri, Mohammad Shekari

“…We performed a case-control analysis to investigate this relationship within a population from southern Iran. DNA samples were isolated from 100 cases with schizophrenia and 100 normal controls. …”
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Molecular characterization and genome sequencing of selected highly resistant clinical isolates of Pseudomonas aeruginosa and its association with the clustered regularly interspac... by Hekmat A. Owaid, Mushtak T.S. Al-Ouqaili

“…Further, WGS was conducted using a DNA nanoball sequencing platform via BGI-Tech for the isolates of interest. …”
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