Duplex qPCR for detecting and differentiating porcine epidemic diarrhea virus GI and GII subtypes by Bin Wang, Wang Han, Di Wu, Yue Jing, Li Ma, Feiyang Jiang, Shusen Ji, Lianmei Bai, Xiuju Yu, Wei Hou, Shouyu Wang, Haidong Wang

“…Leveraging this information, we developed a TaqMan probe-based quantitative real-time PCR (qPCR) assay that is uniquely tailored to detect both PEDV GI and GII subtypes.ResultsAdditionally, this qPCR can identify PEDV GI and GII subtypes with high sensitivities of 90 copies/μL and 40 copies/μL, respectively (refers to the number of copies of the DNA target per microliter of template in the reaction system), much higher than the previously reported works and especially suitable for early diagnosis and prevention. …”
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干扰素-α/β体外增敏替莫唑胺对MGMT阳性胶质瘤干细胞作用

“…【目的】 探讨干扰素是否能增加替莫唑胺(TMZ)对O6甲基鸟嘌呤DNA甲基转移酶(MGMT)阳性胶质瘤干细胞的抗肿瘤作用及其可能机制。【方法】采用“悬浮克隆球形成法”对常规培养条件下MGMT阴性表达的胶质瘤细胞株U251、SKMG-4进行诱导,获得MGMT阳性的胶质瘤干细胞U251G、SKMG-4G;应用CCK-8法检测干扰素-α和干扰素-β联合替莫唑胺对MGMT阳性胶质瘤干细胞的杀伤效应;分别应用逆转录PCR(RT-PCR)、Western-blot检测干扰素-α/β作用后,MGMT阳性胶质瘤干细胞MGMT?…”
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Preferences of pregnant women and obstetric healthcare professionals on using noninvasive prenatal testing to predict adverse pregnancy outcomes: a discrete choice experiment by Mireille N Bekker, Ewoud Schuit, Ian Smith, Ellis Becking, Elisabeth Akkerman, Peter Scheffer, Daphne Stemkens, Caroline J Bax, Neeltje Crombag, Lidewij Henneman

“…Objectives Noninvasive prenatal testing (NIPT) to screen for fetal aneuploidies by analysing cell-free DNA in maternal plasma is available to pregnant women worldwide. …”
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Environmental maternal exposures and the risk of premature birth and intrauterine growth restriction: The Generation Gemelli study protocol of newborn exposome. by Leonardo Villani, Angelo Maria Pezzullo, Roberta Pastorino, Alessandra Maio, Francesca Stollagli, Chiara Tirone, Marta Barba, Angela Maria Cozzolino, Denise Pires Marafon, Martina Porcelli, Annamaria Sbordone, Maria Letizia Patti, Anthea Bottoni, Angela Paladini, Simona Fattore, Domenico Marco Romeo, Ornella Parolini, Wanda Lattanzi, Guido Rindi, Luca Tamagnone, Marco Marazza, Maurizio Genuardi, Elisabetta Tabolacci, Eugenio Maria Mercuri, Antonio Chiaretti, Tina Pasciuto, Maurizio Sanguinetti, Vincenzo Valentini, Giovanni Scambia, Walter Ricciardi, Giovanni Vento, Antonio Lanzone, Stefania Boccia

“…We will perform laboratory examinations including dosage of heavy metals and essential elements, investigation of placental distress and fetal brain damage of biomarkers, analysis of microbiota and of DNA methylation profile. We will conduct clinical follow-up assessments in both cases and controls at months 12 and 24 and we will collect anthropometric data, feeding types with particular reference to breastfeeding and its duration, pediatric emergency room visits, hospitalizations, medication usage, known allergies, and neuropsychological development.…”
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Disease-associated genetic variants in the regulatory regions of human genes: mechanisms of action on transcription and genomic resources for dissecting these mechanisms by E. V. Ignatieva, E. A. Matrosova

“…A triggering event mediating the effect of a pathogenic genetic variant on the level of gene expression can be, for example, a change in the functional activity of transcription factor binding sites (TFBSs) or DNA methylation change, which, in turn, affects the functional activity of promoters or enhancers. …”
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Altered expression profile of plasma exosomal microRNAs in exclusive electronic cigarette adult users by Dongmei Li, Zidian Xie, Sadiya Bi Shaikh, Irfan Rahman

“…Overexpression of hsa-miR-125b-5p promoted DNA damage in bronchial epithelial cells. These findings suggest an elevation of carcinogenic cellular signaling pathways in exclusive e-cigarette users.…”
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Dysbiosis of Gastric Mucosal Fungal Microbiota in the Gastric Cancer Microenvironment by Zhenzhan Zhang, Hao Feng, Yaopeng Qiu, Zhou Xu, Qingfeng Xie, Wenfu Ding, Hao Liu, Guoxin Li

“…Gastric fungal microbiome profiling was performed with internal transcribed spacer (ITS) rDNA sequencing in primary tumor and corresponding paired normal mucosal tissues from 61 GC patients. …”
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Kojic Acid Derivative as an Antimitotic Agent That Selectively Kills Tumour Cells by Giuseppina Pichiri, Marco Piludu, Terenzio Congiu, Nicole Grandi, Pierpaolo Coni, Monica Piras, Mariusz Jaremko, Joanna Izabela Lachowicz

“…There are a few approaches that may be used to meet this objective, mainly through inhibiting DNA replication or mitosis. Despite intensive studies on new chemotherapeutics, the biggest problem remains the side effects associated with the inhibition of cell division in non-tumoural host cells. …”
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Analysis of Biomolecular Changes in HeLa Cervical Cancer Cell Line Induced by Interaction with [Pd(dach)Cl₂] by Vanja Ralić, Maja D. Nešić, Tanja Dučić, Milutin Stepić, Lela Korićanac, Katarina Davalieva, Marijana Petković

“…A spectral analysis, complemented by statistics, revealed that the Pd(II) complex considerably affected all major types of macromolecules in HeLa cells and induced structural changes in proteins through an increased formation of cross-β-sheets and causes structural rearrangement in deoxyribonucleic acid (DNA) through potential chromosome fragmentation. …”
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Genomic characterization of chronic lymphocytic leukemia in patients of African ancestry by Cecilia Bonolo de Campos, Chantal E. McCabe, Laura A. Bruins, Daniel R. O’Brien, Sochilt Brown, Renee C. Tschumper, Cristine Allmer, Yuan Xiao Zhu, Kari G. Rabe, Sameer A. Parikh, Neil E. Kay, Huihuang Yan, James R. Cerhan, John N. Allan, Richard R. Furman, J. Brice Weinberg, Danielle M. Brander, Diane F. Jelinek, Marta Chesi, Susan L. Slager, Esteban Braggio

“…We sequenced 59 putative driver genes and found an increased frequency of high-impact mutations in AA CLL, including genes of the DNA damage repair (DDR) pathway. Telomere erosion was also increased in AA CLL, amplifying the notion of increased genomic instability in AA CLL. …”
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Identification of a Ferroptosis-Related Signature Associated with Prognosis and Immune Infiltration in Adrenocortical Carcinoma by Xi Chen, Lijun Yan, Feng Jiang, Yu Lu, Ni Zeng, Shufang Yang, Xianghua Ma

“…GSEA revealed that gene sets correlated with biological processes including cell cycle, DNA replication, base excision repair, and P53 signaling pathway were highly enriched in high-risk group. …”
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Role of Sex Hormones in the Development and Progression of Hepatitis B Virus-Associated Hepatocellular Carcinoma by Maurizio Montella, Giovanni D’Arena, Anna Crispo, Mario Capunzo, Flavia Nocerino, Maria Grimaldi, Antonio Barbieri, Anna Maria D’Ursi, Mario Felice Tecce, Alfonso Amore, Massimiliano Galdiero, Gennaro Ciliberto, Aldo Giudice

“…Additionally, sex hormones can also affect HBV-related hepatocarcinogenesis by inducing epigenetic changes such as the regulation of mRNA levels by microRNAs (miRNAs), DNA methylation, and histone modification in liver tissue. …”
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Tumor Necrosis Factor Receptor Superfamily, Member 1B Haplotypes Increase or Decrease the Risk of Inflammatory Bowel Diseases in a New Zealand Caucasian Population by Lynnette R. Ferguson, Dug Yeo Han, Claudia Huebner, Ivonne Petermann, Murray L. Barclay, Richard B. Gearry, Alan McCulloch, Pieter S. Demmers

“…This study investigates the role that selected single nucleotide polymorphisms (SNPs) and haplotypes in the TNF-alpha receptor (TNSFRSF1B) gene play in the risk of IBD in a New Zealand Caucasian population. DNA samples from 388 CD, 405 UC, 27 indeterminate colitis patients, and 293 randomly selected controls, from Canterbury, New Zealand were screened for 3 common SNPs in TNSFRSF1B: rs1061622 (c.676T>C), rs1061624 (c.∗1663A>G), and rs3397 (c.∗1690T>C), using TaqMan technologies. …”
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Bile acids in the lower airways is associated with airway microbiota changes in chronic obstructive pulmonary disease: an observational study by Peter A B Wark, Siobhain Mulrennan, Stephen Stick, Yuben P Moodley, Kristy S Nichol, Jose A Caparros-Martin, Montserrat Saladié, S Patricia Agudelo-Romero, F Jerry Reen, Fergal O’Gara

“…BAs were profiled and quantified in BAL supernatants using mass spectrometry. Microbial DNA was extracted from BAL pellets and quantified using quantitative PCR. …”
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Nisin Z Production by Wild Strains of Lactococcus lactis Isolated from Brazilian (Italian Type) Fermented Sausage by Margarete Alice Fontes Saraiva, Dagim Jirata Birri, Dag Anders Brede, Maria Cristina Baracat-Pereira, Marisa Vieira de Queiroz, Ingolf F Nes, Célia Alencar de Moraes

“…The five isolates produced different amounts of the bacteriocin, L. lactis ID1.5 and ID8.5 isolates being the best nisin Z producers. DNA sequence analysis did not reveal any sequence differences in the nisZ and nisF promoter regions that could explain the differences in nisin Z production, suggesting that there should be other factors responsible for differential nisin Z production by the isolates.…”
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Epigenetics of suicidal behavior by R. N. Mustafin, A. V. Kazantseva, R. F. Enikeeva, Yu. D. Davydova, S. B. Malykh, V. V. Viktorov, E. K. Khusnutdinova

“…The analysis of a brain obtained from suicide victims, representing a unique tool for the analysis of modified genomic processes, revealed a wide range of reprogramming patterns of DNA methylation in promoters of the genes of polyamine (OAZ1, OAZ2, AMD1, ARG2, SKA2), serotonergic (SLC6A4) and GABAergic (GABRA1) systems, HPA-axis (GR, NR3C1), tyrosine kinase (TrkB) receptors, brain-derived neurotrophic factor (BDNF). …”
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Genetic modification of the shikimate pathway to reduce lignin content in switchgrass (Panicum virgatum L.) significantly impacts plant microbiomes by Shuang Liu, Ming-Yi Chou, Gian Maria Niccolò Benucci, Aymerick Eudes, Gregory Bonito

“…We investigated how QsuB expression influenced switchgrass-associated fungal and bacterial communities using high-throughput Illumina MiSeq amplicon sequencing of ITS and 16S rDNA. Compared to wild-type, QsuB-engineered switchgrass hosted different microbial communities in roots, rhizosphere, and leaves. …”
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Graphene nanomaterial-based electrochemical biosensors for salivary biomarker detection: A translational approach to oral cancer diagnostics by D. Mahalakshmi, J. Nandhini, G. Meenaloshini, E. Karthikeyan, KK Karthik, J. Sujaritha, Vandhana, V, C. Ragavendran

“…We discuss the unique advantages of saliva as a diagnostic medium and highlight the key salivary biomarkers associated with oral cancer, including proteins, DNA, and RNA. Various electrochemical detection techniques, such as cyclic voltammetry, differential pulse voltammetry, electrochemical impedance spectroscopy, field-effect transistors, amperometry, chronoamperometry, and photoelectrochemical methods, are explored in the context of graphene-based biosensors. …”
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<i>CCND1</i> and <i>PTEN</i> genes as markers of progression in vulvar cancer by A. V. Busarova, N. A. Petrusenko, P. A. Kruze, N. V. Porhanova

“…The study included 50 patients aged 29–80 years with the diagnosis of squamous cell carcinoma of the vulva without metastases – group 1 (n = 25) and with metastases to the lymph nodes – group 2 (n = 25). Total DNA from paraffin blocks of tumor and conditionally healthy vulvar tissue served as the material for the study. …”
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RNA sequence analysis landscape: A comprehensive review of task types, databases, datasets, word embedding methods, and language models by Muhammad Nabeel Asim, Muhammad Ali Ibrahim, Tayyaba Asif, Andreas Dengel

“…To enable large-scale RNA sequence analysis, empowerment of wet-lab experimental methods with Artificial Intelligence (AI) applications necessitates scientists to have a comprehensive knowledge of both DNA and AI fields. While molecular biologists encounter challenges in understanding AI methods, computer scientists often lack basic foundations of RNA sequence analysis tasks. …”
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