Hairy transcriptional repression targets and cofactor recruitment in Drosophila. by Daniella Bianchi-Frias, Amir Orian, Jeffrey J Delrow, Julio Vazquez, Alicia E Rosales-Nieves, Susan M Parkhurst

“…Each of the proteins was tethered to Escherichia coli DNA adenine methyltransferase, permitting methylation proximal to in vivo binding sites in both Drosophila Kc cells and early embryos. …”
Get full text

Telomere Length and Oxidative Damage in Children and Adolescents with Autism Spectrum Disorder: A Systematic Review and Meta-Analysis by Leping Ma, Cui Liu, Ran Song, Yeping Qian, Feng Zhang

“…Pooled results showed that compared with controls, children and adolescents with ASD were associated with significantly shorter TL (SMD = –0.48; 95% CI = –0.66– –0.29; p < 0.001; particularly in males), lower total antioxidant capacity (TAC: SMD = –1.15; 95% CI = –2.01– –0.30; p = 0.008), and higher oxidative DNA (8-hydroxy-2′-deoxyguanosine, 8-OHdG: SMD = 0.63; 95% CI = 0.03–1.23; p = 0.039), lipid (hexanolyl-lysine, HEL: SMD = 0.37; 95% CI = 0.13–0.62; p = 0.003), and protein (3-nitrotyrosine, 3-NT: SMD = 0.86; 95% CI = 0.21–1.51; p = 0.01; dityrosine, DT: SMD = 0.66; 95% CI = 0.521–0.80; p < 0.01) damage. …”
Get full text

Capecitabine enhances sensitivity to oxaliplatin in advanced gastric cancer and the effects on patients’ FOXP1 and GGT levels by Xinyu Guo, Yi Liu

“…Further analysis showed that capecitabine combined with oxaliplatin down-regulated the expression of DNA repair related-proteins and up-regulated the expression of key molecules of the apoptosis pathway, thus enhancing the killing effect of oxaliplatin on gastric cancer cells (P < 0.05). …”
Get full text

Molecular genetic and bacteriological methods of bovine mycoplasmosis diagnosis by E. V. Remizova, A. V. Gorbatov, L. K. Semina, Z. A. Skulyabina, N. V. Shmidt, G. A. Baldicheva, N. N. Avduevskaya

“…For that, 177 samples were collected from animals with mycoplasmosis clinical signs, 45 of them were tested using molecular genetic method, 132 samples were subjected to bacteriological testing. Mycoplasma DNA was detected in 71.1% of samples, and specific colonies were detected in 3.8% of samples. …”
Get full text

Intestinal flora composition and fecal metabolic phenotype in elderly patients with sleep disorders combined with type 2 diabetes by Zhuohao Yin, Huaze Xie, Fuyuan Liu, Xue Kong, Wei Chen, Yangfan Gong, Wei Ge

“…The composition feature of intestinal flora and metabolomics in the ESdD group was described through high‐throughput 16S rDNA sequencing and nontargeted analysis based on liquid chromatography–mass spectrometry. …”
Get full text

Unexpected species diversity in the understanding of selenium-containing soil invertebrates by Bin Mao, XiangLiang Fang, HongLing Lei, YunLi Xiao, Yue Fu

“…This study utilized environmental DNA metabarcoding to investigate the interrelations among environmental factors, soil invertebrate diversity, and community characteristics concerning soil Se content, pH, and moisture content in the region. …”
Get full text

Lymphocyte Oxidative Stress/Genotoxic Effects Are Related to Serum IgG and IgA Levels in Coke Oven Workers by Meili Gao, Yongfei Li, Aqun Zheng, Xiaochang Xue, Lan Chen, Yu Kong

“…These data suggest that oxidative stress/DNA damage induced by PAHs may play a role in toxic responses for PAHs in immunological functions.…”
Get full text

Anti-Apoptosis Effects of Green Tea in Diagnostic and Therapeutic Doses of Ionizing Radiation by Najmeh Anbiaee, Mohammad Taghi Bahreyni Toossi, Hosein Azimian, Zoha Sahebnasagh, Sepideh Abdollahi Dehkordi, Majid Kianmehr, samaneh soudmand salarabadi, Maryam Najafi Amiri

“…Radioprotective agents have the potential to decrease DNA damage and free radicals produced by ionizing radiation. …”
Get full text

Triatoma venosa and Panstrongylus geniculatus challenge the certification of interruption of vectorial Trypanosoma cruzi transmission by Rhodnius prolixus in eastern Colombia. by Omar Cantillo-Barraza, Lídia Gual-González, Natalia Velásquez-Ortiz, Manuel Alfonso Medina Camargo, Paola González, Lissa Cruz-Saavedra, Adriana Castillo, Sara Zuluaga, Giovanny Herrera, Hanson Cowan, Andrés Velez-Mira, Luz Helena Patiño, Juan David Ramírez, Omar Triana, Melissa S Nolan

“…No evidence of T. cruzi DNA was found using qPCR in human blood samples, but we found high parasitemia levels in the infected dogs. …”
Get full text

Comparative Analysis of Embryonic Development and Antioxidant Stress Response in Hybrid Grouper (<i>Epinephelus fuscoguttatus</i> ♀ × <i>E. lanceolatus</i> ♂) with Different Ploidy... by Haizhan Tang, Yang Li, Meng Zhang, Xin Fan, Jian Luo, Xin Wen

“…Flow cytometry showed triploids that had 1.55 times more DNA and larger red blood cells. Triploids had lower midgut trypsin and lipase levels than diploids (<i>p</i> < 0.05) but similar glucose, liver malondialdehyde, and total cholesterol levels (<i>p</i> > 0.05). …”
Get full text

A comparative analysis of sequence composition in different lots of a phage display peptide library during amplification by Anders Wilgaard Sinkjaer, Ane Beth Sloth, Amanda Oester Andersen, Malte Jensen, Babak Bakhshinejad, Andreas Kjaer

“….™-12 phage display library. DNA from the samples was subjected to Next-Generation Sequencing (NGS) using an Illumina platform. …”
Get full text

Lipopolysaccharide-Binding Protein Downregulates Fractalkine through Activation of p38 MAPK and NF-κB by Xia Huang, Yi Zeng, Yujie Jiang, Yueqiu Qin, Weigui Luo, Shulin Xiang, Suren R. Sooranna, Liao Pinhu

“…A549 cells were transfected with LBP or LBP shRNA plasmid DNA or pretreated with SB203580 or SC-514 following LPS treatment. …”
Get full text

Prevalence of nasopharyngeal bacteria during naturally occurring bovine respiratory disease in commercial stocker cattle by Afroza Akter, Hannah Teddleton, Marc Caldwell, Gina Pighetti, Phillip R. Myer, Madison T. Henniger, Liesel Schneider, Elizabeth Shepherd

“…On days 0, 7, 14, and 21, NP samples were collected, and subsequent DNA were isolated and sequenced. After sequencing, 16S rRNA V4 gene was amplified and utilized for NP bacterial determination. …”
Get full text

Molecular Mechanisms of Neurodegeneration Related to C9orf72 Hexanucleotide Repeat Expansion by Mirjana Babić Leko, Vera Župunski, Jason Kirincich, Dinko Smilović, Tibor Hortobágyi, Patrick R. Hof, Goran Šimić

“…However, many more cellular processes are affected by pathological processes in C9FTD/ALS, including nucleocytoplasmic transport, RNA processing, normal function of nucleolus, formation of membraneless organelles, translation, ubiquitin proteasome system, Notch signalling pathway, granule transport, and normal function of TAR DNA-binding protein 43 (TDP-43). Although the exact molecular mechanisms through which C9orf72 repeat expansions account for neurodegeneration have not been elucidated, some potential therapeutics, such as antisense oligonucleotides targeting hexanucleotide GGGGCC repeats in mRNA, were successful in preclinical trials and are awaiting phase 1 clinical trials. …”
Get full text

Comparative proteomics analysis reveals the domesticated Lepista sordida primordium differentiation regulation mechanism and the subsequent different development patterns in the pi... by Xiuqing Yang, Meijie Liu, Yanjiao Zhang, Lizhong Guo, Junqi Wang, Sizhu Li, Yan Zhang, Xiaobo Li, Lili Xu, Hao Yu

“…The highly expressed proteins related to transcription, RNA splicing, the production of various organelles, DNA conformational change, nucleosome organization, protein processing, maturation and transport, and cell detoxification regulated the pileus development and maturity. …”
Get full text

Gene Expression Profile in Delay Graft Function: Inflammatory Markers Are Associated with Recipient and Donor Risk Factors by Diego Guerrieri, Luis Re, Jorgelina Petroni, Nella Ambrosi, Roxana E. Pilotti, H. Eduardo Chuluyan, Domingo Casadei, Claudio Incardona

“…Seven days after kidney transplant, a cDNA microarray was performed on biopsies of graft from patients with and without DGF. …”
Get full text

Effects of orally administered clioquinol on the fecal microbiome of horses by Mikaila Z. Smith, Mary York, Kile S. Townsend, Lynn M. Martin, Tamara Gull, Lyndon M. Coghill, Aaron C. Ericsson, Philip J. Johnson

“…Libraries of 16S rRNA V4 region amplicons generated from fecal DNA were sequenced using the Illumina sequencing platform. …”
Get full text

Magnolol induces cell death through PI3K/Akt‐mediated epigenetic modifications boosting treatment of BRAF‐ and NRAS‐mutant melanoma by Abdullah Al Emran, Brinda Reddy Chinna Chowdary, Farzana Ahmed, Heinz Hammerlindl, Antje Huefner, Nikolas K. Haass, Wolfgang Schuehly, Helmut Schaider

“…Alongside, the repressive histone mark H3K9me3 was increased as a response to DNA damage. Magnolol‐induced alterations of histone modifications are reversible upon activation of the Akt pathway. …”
Get full text

A High Resolution Melting Analysis (HRM) PCR assay for the detection and identification of Old World Leishmania species. by Yusr Saadi-Ben Aoun, Hejer Souguir, Hamed Chouaieb, Mongia Kraiem, Insaf Bel Hadj Ali, Ahmed S Chakroun, Florian Noguier, Akila Fathallah-Mili, David Piquemal, Ikram Guizani

“…Accurate species identification is crucial for effective diagnosis, treatment, and control of these diseases, but traditionally relies on DNA-based methods. High Resolution Melting analysis PCR (HRM PCR) provides rapid results and precise differentiation based on nucleotide variations. …”
Get full text

Effect of Selenate on Viability and Selenomethionine Accumulation of Chlorella sorokiniana Grown in Batch Culture by Živan Gojkovic, Carlos Vílchez, Rafael Torronteras, Javier Vigara, Veronica Gómez-Jacinto, Nora Janzer, José-Luis Gómez-Ariza, Ivana Márová, Ines Garbayo

“…Unlike control cultures, SDS PAGE electrophoresis of crude extracts from selenate-exposed cell cultures revealed appearance of a protein band identified as 53 kDa Rubisco large subunit of Chlorella sorokiniana, suggesting that selenate affects expression of the corresponding chloroplast gene as this subunit is encoded in the chloroplast DNA. Results revealed that the microalga was able to accumulate up to 140 mg·kg−1 of SeMet in 120 h of cultivation. …”
Get full text