Metabolic and epigenetic regulation of macrophage polarization in atherosclerosis: Molecular mechanisms and targeted therapies by Pinglian Yang, Xiaoling Rong, Zhechang Gao, Jiaojiao Wang, Zhiping Liu

“…With the advance of metabolomics and epigenetics, metabolites/metabolic and epigenetic modification such as DNA methylation, histone modification and noncoding RNA, play a crucial role in macrophage polarization and the progression of atherosclerosis. …”
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Two novel entomopathogenic fungal species of Lecanicillium isolated from soil in China by Tongyi Liu, Wei Chen, Yanhong Tang, Fen Liu, Liyan Yao, Qiongbo Hu, Ke Zhang, Qunfang Weng

“…They differ from known closely related species primarily in sporulation structure, conidia and colony morphological characteristics, and sequence variations in the LSU, SSU, ITS, and TEF gene regions of the ribosomal DNA. Multigene phylogenetic analysis has provided strong molecular evidence supporting the classification of the strains, AH13B2 and JX15A210 within the genus Lecanicillium. …”
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High throughput single cell metagenomic sequencing with semi-permeable capsules: unraveling microbial diversity at the single-cell level in sewage and fecal microbiomes by Meilee Ling, Judit Szarvas, Vaida Kurmauskaitė, Vaidotas Kiseliovas, Rapolas Žilionis, Rapolas Žilionis, Baptiste Avot, Baptiste Avot, Patrick Munk, Frank M. Aarestrup

“…Our method involves extracting and amplifying single bacterial DNA within individual SPCs, followed by combinatorial split-and-pool single-amplified genome (SAG) barcoding and short-read sequencing. …”
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Characteristics of gut microbiota profiles in Asian elephants (Elephas maximus) with gastrointestinal disorders by Sarisa Klinhom, Chanon Kunasol, Sirawit Sriwichaiin, Sasiwan Kerdphoo, Nipon Chattipakorn, Siriporn C. Chattipakorn, Chatchote Thitaram

“…The samples were subjected to DNA extraction and amplification targeting the V3-V4 region of 16S rRNA gene for next-generation sequencing analysis. …”
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Deployment of next-generation sequencing approach for variant detection in myocardial infarction: A concise investigation by Desaraju Suresh Bhargav, Nadeem Siddiqui, Bhadra Murthy V, Vijaya Tartte, Vanaja V

“…After taking written informed consent, DNA samples were collected and subjected to NGS sequencing studies; a 17-gene customized MI panel was designed for targeted sequencing. …”
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First two mitochondrial genomes for the order Filobasidiales reveal novel gene rearrangements and intron dynamics of Tremellomycetes by Qiang Li, Zhijie Bao, Ke Tang, Huiyu Feng, Wenying Tu, Lijiao Li, Yunlei Han, Mei Cao, Changsong Zhao

“…The mitogenomes of F. wieringae and F. globisporum both comprised circular DNA molecules, with sizes of 27,861 bp and 71,783 bp, respectively. …”
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Therapeutic Potential of Herbal Medicines in Combating Particulate Matter (PM)-Induced Health Effects: Insights from Recent Studies by Aekkhaluck Intharuksa, Warunya Arunotayanun, Mingkwan Na Takuathung, Yaowatat Boongla, Siripat Chaichit, Suthiwat Khamnuan, Anchalee Prasansuklab

“…Upon inhalation, ingestion, or dermal contact, PM can penetrate biological systems, inducing oxidative stress, inflammation, and DNA damage, which contribute to a range of health complications. …”
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Actinidia deliciosa as a complemental therapy against nephropathy and oxidative stress in diabetic rats by Ali Y. Naoom, Wenyi Kang, Nora F. Ghanem, Mohamed M. Abdel-Daim, Fatma M. El-Demerdash

“…Furthermore, histological, immunohistochemical alpha-smooth muscle actin immunoreactivity (α-SMA-ir) and histochemical (collagen, total protein, DNA, and RNA) alterations were observed in rat kidneys. …”
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Deregulation mechanisms and therapeutic opportunities of p53-responsive microRNAs in diffuse large B-cell lymphoma by Elena N. Voropaeva, Yuriy L. Orlov, Anastasia B. Loginova, Olga B. Seregina, Vladimir N. Maksimov, Tatiana I. Pospelova

“…We analyze various molecular mechanisms leading to this dysregulation, including mutations in the TP53 gene, DNA methylation, changes in host-genes expression or microRNA gene copy number, mutations in microRNA and microRNA biogenesis genes.…”
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黏着斑激酶酪氨酸磷酸化在牵拉依赖的细胞形态改变中的作用 by 王连荣, 王菊光, 吴伟康

“…利用PCR 法从人的脐静脉内皮细胞获得FAK cDNA 。通过直接点突变, 利用重叠PCR 制成突变的FAK(F397Y, F925Y)。将FAK 突变转染到细胞内, 使细胞遭受牵拉, 牵拉0 min 为对照组, 牵拉5 、10、20 、30、60 min 为牵拉组, 实验重复3 次, 实验数据利用方差分析和多重比较进行分析。…”
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Harnessing the synergy of Urochloa brizantha and Amazonian Dark Earth microbiomes for enhanced pasture recovery by Anderson Santos de Freitas, Luís Felipe Guandalin Zagatto, Gabriel Silvestre Rocha, Franciele Muchalak, Guilherme Lucio Martins, Solange dos Santos Silva-Zagatto, Rogério Eiji Hanada, Aleksander Westphal Muniz, Siu Mui Tsai

“…We conducted a comprehensive plant succession experiment in the greenhouse, utilizing vase soil samples for next-generation sequencing of 16 S rDNA, enzymatic activity assays, and soil chemical properties analysis. …”
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Molecular subtype of ovarian clear cell carcinoma: an analysis of 80 Chinese patients using the TCGA molecular classification of endometrial cancer by Wei Chen, Lu Yan, Qin Li, Shuling Zhou, Ting Hou, Huijuan Yang, Shuang Ye

“…Abstract Background To assess the utility of the TCGA molecular classification of endometrial cancer in a well-annotated, moderately sized, consecutive cohort of Chinese patients with ovarian clear cell carcinoma (OCCC). Methods We performed DNA sequencing on 80 OCCC patients via a panel that contains 520 cancer-related genes. …”
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Design and expression of a chimeric recombinant antigen (SsIR-Ss1a) for the serodiagnosis of human strongyloidiasis: Evaluation of performance, sensitivity, and specificity. by Mostafa Omidian, Zohreh Mostafavi-Pour, Marzieh Asadi, Meysam Sharifdini, Navid Nezafat, Ali Pouryousef, Amir Savardashtaki, Mortaza Taheri-Anganeh, Fattaneh Mikaeili, Bahador Sarkari

“…The construct was expressed into the pET-23a (+) expression vector and the optimized DNA sequences of SsIR-Ss1a (873 bp) were cloned into competent E. coli DH5α cells. …”
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Pathological findings in rainbow trout (oncorhynchus mykiss, walbaum 1792) in experimental vagococcus salmoninarum infection by S. Serap Birincioğlu, Hamdi Avcı, T. Tansel Tanrikulu, E.Tuğrul Epikmen, M. Lütfi Avsever

“…Tissue sections stained with haematoxylin-eosin and examined using light microscope. DNA extractions of the isolate were made for the molecular analysis of the bacteria and then, specific primers were used to identify V. salmoninarum. …”
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Genetic Diversity of <i>Diaphorina citri</i> and Its Endosymbiont Across Diffusion Frontier and Epidemic Areas of Citrus Huanglongbing in China by Jin Yang, Ai-Jun Huang, Jun Zhou, Ping You, Xiang Li, Han Luo, Long Yi

“…We utilized mitochondrial genes (<i>COI</i>, <i>ND5</i>, and <i>Cytb</i>) of <i>D. citri</i> and housekeeping genes (<i>dnaQ</i>, <i>rpoC</i>, and <i>argH</i>) of its endosymbiont <i>Candidatus</i> Carsonella ruddii (<i>Ca.…”
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Comparisons of Differentiation Potential in Human Mesenchymal Stem Cells from Wharton’s Jelly, Bone Marrow, and Pancreatic Tissues by Shih-Yi Kao, Jia-Fwu Shyu, Hwai-Shi Wang, Chi-Hung Lin, Cheng-Hsi Su, Tien-Hua Chen, Zen-Chung Weng, Pei-Jiun Tsai

“…The in vitro differential function of these IPCs was compared by insulin-to-DNA ratios and C-peptide levels after glucose challenge. …”
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梅花鹿<italic>DLX5</italic>基因克隆及表达分析 by 王鹏, 刘方政, 李萱博, 王春花, 于海浩, 夏彦玲

“…为研究梅花鹿（<italic>Cervus nippon</italic>）<italic>DLX5</italic>基因的结构和功能，进一步探究其与梅花鹿茸角骨化机制间的关系，采用RT-PCR技术对梅花鹿<italic>DLX5</italic>基因进行克隆，获得包含全部编码区的cDNA序列，对该基因的氨基酸序列进行生物信息学分析并构建系统进化树，通过KEGG富集分析其信号通路，运用实时荧光定量RT-PCR检测该基因在鹿茸生长不同时期的表达情况。…”
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CaGe: A Web-Based Cancer Gene Annotation System for Cancer Genomics by Young-Kyu Park, Tae-Wook Kang, Su-Jin Baek, Kwon-Il Kim, Seon-Young Kim, Doheon Lee, Yong Sung Kim

“…High-throughput genomic technologies (HGTs), including next-generation DNA sequencing (NGS), microarray, and serial analysis of gene expression (SAGE), have become effective experimental tools for cancer genomics to identify cancer-associated somatic genomic alterations and genes. …”
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LINE-1 methylation status and survival outcomes in colorectal cancer patients: A systematic review and meta-analysis by Akbar Bagheri, Tohid Jafari-Koshki, Leila Alizadeh, Mortaza Raeisi, Yaghoub Moaddab, Abbas Karimi

“…Colorectal cancer (CRC) is a leading cause of cancer-related mortality worldwide. DNA hypomethylation, particularly of Long Interspersed Nuclear Element-1 (LINE-1) repetitive sequences, has been implicated in CRC development and progression. …”
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Revolutionizing radiotherapy: gold nanoparticles with polyphenol coating as novel enhancers in breast cancer cells—an in vitro study by Simona Tarantino, Annalisa Bianco, Mariafrancesca Cascione, Alessandra Carlà, Lia Fiamà, Riccardo Di Corato, Livia Giotta, Paolo Pellegrino, Anna Paola Caricato, Rosaria Rinaldi, Valeria De Matteis

“…These cells were exposed to varying doses of X-ray irradiation, and the study assessed cell viability, morphological changes and DNA damage. The results showed that green-synthesized AuNPs significantly enhanced the therapeutic effects of radiotherapy at lower radiation doses, indicating their potential as a valuable addition to breast cancer treatment.…”
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