Isolation and characterization of a novel lytic phage K14-2 infecting diverse species of the genus Klebsiella and Raoultella by Seomin Kang, Jeong-Eun Han, Young-Sik Choi, In-Chul Jeong, Jin-Woo Bae, Jin-Woo Bae, Jin-Woo Bae

“…The genome of K14-2 was found to be double-stranded DNA, comprising 175,759 base pairs with a GC content of 41.8%. …”
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Continuous Quadrupole Magnetic Separation of Islets during Digestion Improves Purified Porcine Islet Viability by Bradley P. Weegman, Venkata Sunil Kumar Sajja, Thomas M. Suszynski, Michael D. Rizzari, William E. Scott III, Jennifer P. Kitzmann, Kate R. Mueller, Thomas R. Hanley, David J. Kennedy, Paul W. Todd, Appakalai N. Balamurugan, Bernhard J. Hering, Klearchos K. Papas

“…Islets purified by QMS exhibited significantly improved viability (measured by oxygen consumption rate per DNA, p<0.03) and better morphology relative to control islets. …”
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Thapsigargin对人晶状体上皮细胞系SRA01／04增殖抑制的作用机制 by 姬红培, 吴明星, 彭瑞萍

“…【方法】不同浓度TG处理SRA01／04细胞72h，噻唑蓝比色法（MTT）检测细胞增殖；流式细胞仪（PI法）检测细胞周期改变；透射电镜观察细胞超微结构变化；TUNEL法检测细胞核中DNA的断裂情况；激光共焦显微镜和流式细胞仪检测Caspase3活性亚单位（17／19ku）的表达及阳性细胞表达率。…”
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The Th17/Treg ratio, IL-1RA and sCD14 levels in primary HIV infection predict the T-cell activation set point in the absence of systemic microbial translocation. by Mathieu F Chevalier, Gaël Petitjean, Catherine Dunyach-Rémy, Céline Didier, Pierre-Marie Girard, Maria Elena Manea, Pauline Campa, Laurence Meyer, Christine Rouzioux, Jean-Philippe Lavigne, Françoise Barré-Sinoussi, Daniel Scott-Algara, Laurence Weiss

“…No decrease in anti-endotoxin core antibody (EndoCAb) and no peptidoglycan were detected during PHI. In addition, 16S rDNA was only detected at low levels in 2 out 27 patients at baseline and in one additional patient at M6. …”
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Clinicopathological characteristics and gene mutations in 11 patients with lipoprotein glomerulopathy by Yan Qin, Xiao-Jing Sun, Yi-Fang Hu, Meng Jing, Xiao-Juan Yu, Ming-Hui Zhao, Ying Tan

“…This study aimed to investigate APOE gene mutations and the clinicopathological features in eleven LPG patients.Methods Clinicopathological and follow-up data were obtained by extracting DNA, followed by APOE coding region sequencing analysis. …”
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Probing the potential of InDel polymorphisms on the molecular identification of cetaceans by Dafne Adriana Abreu dos Anjos, Rodrigo Goldenberg Barbosa, Anna Luiza dos Santos Donato, Elizeu Fagundes de Carvalho, Cesar Rogerio Leal do Amaral

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Analysis of nucleotide sequences of the <i>GT47</i> glycosyltransferase gene in rye cultivars differing in the content of water-soluble pentosans in grain by E. A. Zaikina, R. R. Kayumova, A. R. Kuluev, R. R. Ismagilov, B. R. Kuluev

“…The aim of this study was amplification, sequencing, and search for single nucleotide substitutions or other mutations in the GT47 gene in various rye cultivars differing in the content of water-soluble pentosans in their grain and the viscosity of their aqueous extract. DNA from rye leaves was isolated by the standard CTAB method. …”
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Poly ADP-Ribosylation in a Plant Pathogenic Oomycete <i>Phytophthora infestans</i>: A Key Controller of Growth and Host Plant Colonisation by Viktoriya O. Samarskaya, Sofya Koblova, Tatiana Suprunova, Eugene A. Rogozhin, Nadezhda Spechenkova, Sofiya Yakunina, Andrew J. Love, Natalia O. Kalinina, Michael Taliansky

“…ADP-ribosylation is a reversible modification of proteins and nucleic acids, which controls major cellular processes, including DNA damage repair, cell proliferation and differentiation, metabolism, stress, and immunity in plants and animals. …”
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Fecal microbiota transplantation alleviates radiation enteritis by modulating gut microbiota and metabolite profiles by Qin Ding, Jing Xue, Nan Li, Zhihui Hu, Jianbo Song

“…Gut microbiota alterations and fecal metabolism were analyzed via 16S rDNA sequencing and targeted metabolomics. The results demonstrated that FMT, particularly in the M-FMT group, effectively alleviated RE by reducing DAI scores, histological damage, and inflammatory markers while enhancing enzyme activity, superoxide dismutase (SOD) levels, and intestinal absorption. …”
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Diploid aposporous sunflower forms triploid BIII progeny displaying increased apospory levels and non-random genetic mutations by Silvina Pessino, Graciela Nestares, Marta B. Bianchi, Iara Katzaroff, Lucía Amato, Marika Bocchini, Gianpiero Marconi, Emidio Albertini, Ana C. Ochogavía

“…Furthermore, specific non-random genetic and DNA methylation changes were detected in the F2 triploids compared to F2 diploids and parental plants, highlighting recurrent (epi)genetic alterations occurring during triploidization. …”
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The effect of thioredoxin and prochymosin coexpression on the refolding of recombinant alpaca chymosin by S. V. Belenkaya, D. N. Shcherbakov, A. I. Chapoval, T. I. Esina, V. V. Elchaninov

“…Another source of chymosin, which does not require the killing of animals, is based on recombinant DNA technology. Recombinant alpaca chymosin has a number of valuable technological properties that make it attractive for use in cheese-making as an alternative to recombinant bovine chymosin. …”
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Analysis of significantly associated regions and candidate genes for dwarf stem trait in Brassica napus based on BSA-sequencing by ZHAO Weiguo, TA Na, WANG Hao, YANG Zelin

“…[Methods] Rapeseed dwarf stem DW1 was used to cross with tall stem lines WH14 and WH20, respectively, and the F1 and F2 generations were used to analyze the genetic mechanism behind the dwarf trait. Meanwhile, two mixed DNA pools, one from extremely tall and the other from dwarf stem lines, constructed from F2 segregated population of dwarf stem DW1 and tall stem WH20 hybrid combination, were sequenced and analyzed base on BSA-seq technology, and the significant association regions and candidate genes associated with dwarf trait were identified. …”
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Guanosine enhances the bactericidal effect of ceftiofur sodium on Streptococcus suis by activating bacterial metabolism by Yue Dong, Xiaona Liu, Shanshan Xiong, Mingyu Cao, Haojie Wu, Long Chen, Mengmeng Zhao, Yadan Zheng, Zhiyun Zhang, Yanyan Liu, Yanhua Li, Qianwei Qu, Chunliu Dong

“…Bacterial-oxidative stress and DNA damage induced by guanosine is also one of the mechanisms by which the antibacterial effect is enhanced. …”
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Modulation of biological activities in adipose derived stem cells by histone deacetylation by Sallam Abdallah, Mouna Tabebi, Sawsan Qanadilo, Neserin Ali, Jing Wang, Pádraig D’Arcy, Wen Zhong, Folke Sjoberg, Moustafa Elmasry, Ahmed El-Serafi

“…Pathway enrichment analysis showed cell cycle, ATP-dependent chromatin remodeling and DNA processes were among the affected pathways. …”
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基于微卫星标记的雪豹种群结构及遗传多样性 by 周颖娜, 李金霖, 马跃, 王震

“…掌握雪豹（<italic>Panthera uncia</italic>）的遗传多样性信息对其保护和管理有重要意义。对104只雪豹个体的组织样品提取DNA，结合文献中提供的70个雪豹基因分型数据，利用9个微卫星多态性位点进行遗传多样性研究。结果显示：当样本量从70个增加到174个时，雪豹种群有分化为2个种群的趋势，即西北部群和中部群；共检测到45个等位基因（<italic>N</italic>_a），中部、西北部2个种群平均每个位点<italic>N</italic>_a分别为4.556和4.667，平均期望杂合度（<italic>H</italic>_e）分别为0.574和0.591，平均观测杂合度（<italic>H</italic>_o）分别为0.517和0.498。…”
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Ellagic Acid Alleviates Rheumatoid Arthritis in Rats through Inhibiting MTA1/HDAC1-Mediated Nur77 Deacetylation by Huanjin Song, Hao Wu, Jun Dong, Sihua Huang, Jintao Ye, Ruoxi Liu

“…Then, TNF-α-treated MH7A cells were incubated with EA alone or together with MTA1 overexpression plasmid (pcDNA-MTA1), and we found that EA inhibited proliferation, inflammation cytokine levels, and oxidative stress marker protein levels and promoted apoptosis in MH7A cells, while MTA1 overexpression abolished these effects. …”
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AMPK Activation in TET2 Downregulated Leukemia Cells Upon Glutamine Limitation by Ahsen Merve BAYRAK, Burcu YUCEL

“…Although glutamine can be produced in cells via a series of enzymatic reactions, a group of cancer cells are dependent on extracellular glutamine for survival. TET2 plays a role in DNA demethylation and is a tumor suppressor gene. …”
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Genome-wide development of simple sequence repeat (SSR) markers at 2-Mb intervals in lotus (Nelumbo Adans.) by Fengluan Liu, Lin Xi, Naifeng Fu

“…., Chr.1-2.5, 4.5, 6.5 Mb) through four steps: online primer design, primer pair evaluation, agarose gel electrophoresis testing using six Asian lotus, one American lotus, and two their hybrids, and DNA sequence alignment. Finally, the polymerase chain reaction (PCR) efficiency of several SSR markers was validated in 20 Asian temperate lotus, eight Asian tropical lotus, and one American lotus. …”
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Clinical relevance and druggability of sole reciprocal kinase fusions: A large‐scale study by Jiao Feng, Tonghui Ma, Chunyang Wang, Baoming Wang, Qian Liu, Zhengchuang Liu, Houquan Tao, Zaiyuan Ye

“…These rare genomic arrangements, despite lacking kinase domains at the DNA level, demonstrated potential RNA‐level druggability in sporadic cases from our prior research. …”
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Unraveling the Beneficial Role of Resveratrol in Fructose-Induced Non-Alcoholic Steatohepatitis with a Focus on the AMPK/Nrf2 Signaling Axis by Soha S. Zakaria, Safaa M. Hanafy

“…ELISA was used to measure Beclin 1, AMPK, IL-6, and the DNA-binding activity of Nrf2. Oxidative stress indicators, including GSH, SOD, and MDA, were evaluated spectrophotometrically. …”
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