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4641
Coexistence of blaMIR-1 and blaNDM-1 resistance genes with a novel ST type in Enterobacter roggenkampii from a stool sample: A genome sequencing study
Published 2025-01-01“…Individual colonies were isolated, and bacterial species were identified using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry and 16S rRNA gene sequencing. Genomic DNA was extracted and sequenced via Illumina and Oxford Nanopore sequencing. …”
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4642
Genetic Diversity and Structure of a Critically Endangered Ornamental Species, <i>Rhododendron farinosum</i>, with Extremely Small Populations
Published 2025-01-01“…To unravel the reasons behind the endangerment of this species and provide guidance for the rational conservation of this species, this study obtained a large number of SNP loci by using double-digest restriction-site-associated DNA sequencing (ddRAD-seq) to evaluate the genetic diversity and genetic structure of <i>R. farinosum</i>, as well as to infer the population history of this species. …”
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4643
Gastric Precancerous Lesions: From Progenitor Cell and Microsatellite Instability to Clinical Interpretation of Gastric Cancer Risk
Published 2024-10-01“…MSI was studied with multiplex PCR evaluation of DNA microsatellites (NR-21, NR-24, NR-27, BAT-25, BAT-26) from paraffin sections, their analysis with capillary electrophoresis. …”
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4644
Selective Binding of Distamycin A Derivative to G-Quadruplex Structure [d(TGGGGT)]4
Published 2010-01-01“…Distamycin A is known to bind to AT-rich sequences of duplex DNA, but it has recently been shown to interact also with G-quadruplexes. …”
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4645
Quantitative expression of estrogen, progesterone and human epidermal growth factor receptor-2 and their correlation with immunohistochemistry in breast cancer at Uganda Cancer Ins...
Published 2025-01-01“…The extracted RNA was quantified using Nanodrop technology, resulting into complementary DNA. ER and PR using IHC were expressed in 60% (n = 12) of the study samples and were negative in 40% (n = 8) of samples. …”
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4646
A Review on Bioactive Phytochemical Constituents and Pharmacological Activities of Aegiceras corniculatum: A Pharmaceutically Important Mangrove Plant
Published 2024-01-01“…The plant-isolated coumaric acid and fatty acids interact with bacterial DNA/RNA and limit protein formation, making them antibacterial. …”
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4647
Dryland fungi are spatially heterogeneous and resistant to global change drivers
Published 2024-12-01“…We assessed fungal communities (using fungal‐specific DNA metabarcoding analyses) of surface soil samples in an in situ global change experiment that included disturbance and drought in a full factorial design in the northern extent of the Chihuahuan Desert. …”
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4648
Insight into the avidity–affinity relationship of the bivalent, pH-dependent interaction between IgG and FcRn
Published 2024-12-01“…As such, we present here a more comprehensive analysis of these interactions with an emphasis on both affinity and avidity by taking advantage of switchSENSE technology, a surface-based biosensor where recombinant FcRn was immobilized via short DNA nanolevers, mimicking the membranous orientation of the receptor. …”
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4649
Holarctic phylogeographic structure of Eurasian wigeon (Mareca penelope)
Published 2019-05-01“…We examined genetic diversity and differentiation of wigeon populations identified with banding data, phylogenetic relationships of mtDNA haplotypes and demographic history of populations and species as a whole by sequencing a 661 base-pair 5’-fragment of the mitochondrial control region from 195 individual ducks collected throughout the Palearctic and Nearctic. …”
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4650
Known and novel parvoviruses identified in domestic pigeons
Published 2025-01-01“…Abstract Background Parvoviridae is a family of single-stranded linear DNA viruses whose members infect both vertebrate and invertebrate species of animals, causing diseases of various systems and often associated with pathology of the gastrointestinal tract. …”
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4651
Comparison of mucosal microbiota populations across the gastrointestinal tract of healthy dogs
Published 2025-01-01“…At that time, mucosal samples from the stomach, duodenum, jejunum, ileum, and mid-colon were collected. DNA was extracted and the hypervariable region 4 (V4) of the 16S rRNA gene was amplified to assess microbiota using Illumina MiSeq sequencing. …”
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4652
Genetic variability and population structure analysis of Protostrongylus oryctolagi (Nematoda: Protostrongylidae) in Lepus europaeus from Central and Northern Italy.
Published 2025-01-01“…Finally, the presence of unconventional shortened amplicons revealed the presence of NUMTs (nuclear copy of mitochondrial genes) in the P. oryctolagi nuclear genome, suggesting caution when using DNA barcode as unique marker for the identification of species belonging to this genus. …”
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4653
Cyclosporine A causes gingival overgrowth via reduced G1 cell cycle arrest in gingival fibroblasts.
Published 2024-01-01“…When cells detect and respond to abnormalities (e.g. DNA damage), cell cycle progression is arrested in the G1 phase until the completion of damage restoration. …”
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4654
Association of the Genetic Polymorphisms in Transcription Factor 7-Like 2 and Peroxisome Proliferator-Activated Receptors-γ2 with Type 2 Diabetes Mellitus and Its Interaction with...
Published 2015-01-01“…We also investigated the interaction of these associations with obesity status. Methods. DNA was extracted from the saliva samples of 272 T2DM patients and 216 nondiabetic Emiratis. …”
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4655
Association between ТР53, MDM2 and NQO1 gene polymorphisms and viral load among women with human papillomavirus
Published 2022-03-01“…Quantitative analysis of HPV DNA was performed by AmpliSens® HPV HCR screen-titer-FRT test system. …”
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4656
Comprehensive guide for epigenetics and transcriptomics data quality control
Published 2025-03-01“…Improved signature discovery, including the identification of the agent and timing of exposure, has been enabled by advancements in assaying techniques to detect RNA expression, DNA base modifications, histone modifications, and chromatin accessibility. …”
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4657
Genome Mining and Characterization of Two Novel <i>Lacticaseibacillus rhamnosus</i> Probiotic Candidates with Bile Salt Hydrolase Activity
Published 2025-01-01“…Gene annotation revealed numerous strain-specific genes involved in metabolism and transport, as well as in DNA recombination. Each strain harbors a single <i>bsh</i> gene, encoding a C-N amide hydrolase, which conserved the essential residues required in the BSH core site. …”
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4658
The use of whole genome amplification for genomic evaluation of bovine embryos
Published 2019-07-01“…The quality and quantity of DNA for all the 78 samples after the whole genome amplification were satisfactory for further genotyping. …”
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4659
MicroRNA-32-5p promotes the proliferation and metastasis of gastric cancer cells
Published 2025-01-01“…Clonal formation, MTT, caspase-3 activity, sperm DNA fragmentation, flow cytometry assay, cell adhesion, transwell assays were performed to detect the functions of miR-32-5p or anti-miR-32-5p on the growth and metastasis of GC cells. …”
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4660
Analysis of a Family with Brugada Syndrome and Sudden Cardiac Death Caused by a Novel Mutation of SCN5A
Published 2022-01-01“…Three generations of a Han Chinese family with Brugada syndrome were recruited in the study; their clinical phenotype data were collected and DNA samples extracted from the peripheral blood. …”
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