Mitochondrial Mayhem: How cigarette smoke induces placental dysfunction through MMS19 degradation by Pei Zhou, Feng Jin, Shenshen Yao, Ben Sun, Ni Sun, Hongbo Guan, Xiaomei Liu

“…MMS19 KD led to the autophagic degradation of several ISC proteins involved in DNA damage repair and mitochondrial respiratory function, thereby inhibiting cell proliferation. …”
Get full text

Association between mild cognitive impairment and APOE Ɛ4, low education level, and lack of spousal relationship in community-dwelling older persons in Nigeria by Isyaku Gwarzo Mukhtar, Mohamed Mabrouk Elkhashab, Muhammad Ali Salim, Salisu Ahmed Ibrahim, Isyaku Umar Yarube

“…APOE was genotyped from peripherally extracted genomic DNA using the sequence-specific primer polymerase chain reaction with Hhal digestion. …”
Get full text

Genetic Variation in the IL-6 and HLA-DQB1 Genes Is Associated with Spontaneous Clearance of Hepatitis C Virus Infection by Paul Ravi Waldron, Ilana Belitskaya-Lévy, Aarthi Chary, Johann Won, Mark Winters, Alexander Monto, James Ryan, Laura C. Lazzeroni, Mark Holodniy

“…Blood was collected for DNA analysis from patients with chronic HCV infection or evidence of spontaneous clearance. …”
Get full text

Molecular and serological investigation of hepatitis B virus in patients with acute undifferentiated febrile illness at Tha Song Yang hospital, Tak Province, Thailand by Narin Thippornchai, Anon Sae-Oueng, Akanitt Jittmittraphap, Wang Nguitragool, Pornsawan Leaungwutiwong

“…Methods A cross-sectional study was conducted using residual serum samples collected between 2016 and 2017 from patients exhibiting symptoms of AUFI at Tha Song Yang Hospital. DNA was extracted and identified for HBV using real-time polymerase chain reaction (PCR). …”
Get full text

Morphophysiological and molecular characterization of millet (Panicum miliaceum L.) varieties for crop improvement in Western Europe by Massimo Vischi, Nicola Zorzin, Maria Bernhart, Johanna Winkler, Dipak Santra, Carla Pappalardo, Stefano Marchetti

“…In parallel, 85 SSR markers were tested on DNA samples extracted from randomly chosen plants of each variety and the 12 responsive markers used to genotype the whole variety set. …”
Get full text

Zebularine Promotes Hepatic Differentiation of Rabbit Bone Marrow Mesenchymal Stem Cells by Interfering with p38 MAPK Signaling by Yong-Heng Luo, Juan Chen, En-Hua Xiao, Qiu-Yun Li, Yong-Mei Luo

“…Demethylating agent zebularine is reported to be capable of inducing differentiation of stem cells by activation of methylated genes, though its function in hepatocyte differentiation is unclear. p38 signal pathway is involved in differentiation of hepatocytes and regulating of DNA methyltransferases 1 (DNMT1) expression. However, little is known about the impact of zebularine on bone marrow mesenchymal stem cells (BMMSCs) and p38 signaling during hepatic differentiation. …”
Get full text

miR-18a过表达及抑制表达人鼻咽癌细胞株的构建 by 常利红, 黄子真, 黄健聪, 陈晓红, 赖晓萍, 张革化

“…【方法】将miR-18a前体的编码基因片段及miR-18a反义寡核苷酸克隆到慢病毒载体中，DNA 测序鉴定；将构建好的载体转染293T细胞，获得重组miR-18a过表达及抑制表达的慢病毒载体，感染人鼻咽癌细 胞CNE1和CNE2，嘌呤霉素筛选获得稳定干扰miR-18a表达及过表达miR-18a的细胞系，PCR及western blot鉴 定。…”
Get full text

Transcriptome profiling revealed multiple circadian rhythm-related genes associated with common gynecological cancers by Lan Peng, Meiping Jiang, Kangming Li, Shuhui Yu, Chunfang Zhao, Lan Zhang, Lan Li

“…BackgroundStudies have shown that more than half of the human genome expression is affected by circadian rhythms, which includes genes involved in cell cycle control, DNA repair and apoptosis that are critical in cancer biology. …”
Get full text

Clan Crypts on the Lower Don of the Early Catacomb Time and Genesis Issues of the Catacomb Rite by Alexey V. Kiyashko

“…A combined method for radiocarbon dating using accelerator mass spectrometry (AMS), DNA analysis of the bones as well as the isotope study of the teeth were carried out in the laboratories of Belfast and Harvard. …”
Get full text

Liquid chromatography/tandem mass spectrometric characterization of Rhodotorula mucilaginosa GMM natural pigment and its synergistic antitumor effect with γ-radiation: in-vitro and... by Ghada S. Ibrahim, Amal I. Hassan, Manal G. Mahmoud, Mohsen M.S. Asker, Mohamed Marzouk

“…The isolate was defined as Rhodotorula mucilaginosa GMM with accession number OQ120277 by 18 S rDNA, and the pigment was characterized by liquid chromatography-mass spectrometric. …”
Get full text

EXO1 is a key gene for lung-resident memory T cells and has diagnostic and predictive values for lung adenocarcinoma by Zhuoqi Li, Xiaoyan Lin, Yuanhui Yang, Mei Tian, Lu Zhang, Fujing Huang, Xiao Wen, Zhigang Wei, Yuan Tian

“…In this study, the RNA expression, DNA methylation, CNV, somatic mutation data of EXO1, and the corresponding patients’ clinical information from publicly available databases were analyzed using bioinformatic methods. …”
Get full text

Flanking monomer repeats define lower context complexity of sites containing single nucleotide polymorphisms in the human genome by N. S. Safronova, M. P. Ponomarenko, I. I. Abnizova, G. V. Orlova, I. V. Chadaeva, Y. L. Orlov

“…The presence of poly-A tracks might be associated with an increased probability of double helix DNA breaks around mutable loci and following fixation of nucleotide changes. …”
Get full text

Point Mutations in the folP Gene Partly Explain Sulfonamide Resistance of Streptococcus mutans by W. Buwembo, S. Aery, C. M. Rwenyonyi, G. Swedberg, F. Kironde

“…The isolates were tested for resistance to cotrimoxazole and their folP DNA (which encodes sulfonamide-targeted enzyme dhps) cloned in pUC19. …”
Get full text

Lake salinization on the Qinghai–Tibetan Plateau alters viral community composition and lifestyles by Yuan-Guo Xie, Yan-Ling Qi, Zhen-Hao Luo, Yan-Ni Qu, Jian Yang, Shi-Qiang Liu, Hai-long Yang, Dan-Wei Xie, Zimeng Wang, Hong-Chen Jiang, Zheng-Shuang Hua

“…Viral genomes encode genes associated with osmotic stress adaptation, DNA recombination, and nutrient transport, which may facilitate host adaptation to saline stress. …”
Get full text

Forensic Support of Pre-Trial Investigative and Inquiry Agencies’ Activities on Combating Crime by O. O. Yukhno

“…The issues of further dissemination and application in law enforcement and expert activities of molecular genetical examinations promising for pre-trial investigation, in particular methods of DNA analysis, have been separately and widely studied. …”
Get full text

Candidate genes for domestication and resistance to cold climate according to whole genome sequencing data of Russian cattle and sheep breeds by N. S. Yudin, D. M. Larkin

“…We used the HapFLK, DCMS, FST and PBS methods to identify DNA regions with signatures of selection. The number of candidate genes in potentially selective regions was 946 in cattle and 151 in sheep. …”
Get full text

New Resinogalea species from Araucaria araucana resin in Chile and reclassification of the genus in the Cryptocaliciomycetidae by Felipe Balocchi, Irene Barnes, Michael J. Wingfield, Rodrigo Ahumada, Cobus M. Visagie

“…A phylogenetic analysis based on the small subunit (SSU), internal transcribed spacer (ITS) and large subunit (LSU) rDNA regions resolved our strains into two clades with Cr. blascoi as its closest relative. …”
Get full text

The SEPALLATA-like gene HrSEP1 in Hippophae rhamnoides regulates flower development by interacting with other MADS-box subfamily genes by Di Cong, Xue Zhao, Xue Zhao, Chang Ni, Mengru Li, Luwen Han, Jianlin Cheng, Hongzhang Liu, Huijing Liu, Dan Yao, Shuying Liu, Guoshuang Chen

“…MADS-box genes are classified into five categories: ABCDE, including SEP1, SEP2, SEP3, SEP4, and other homologous genes, which play important roles in floral organ development. In this study, the cDNA sequence of the HrSEP1 gene was cloned by RT-PCR and confirmed that this gene belongs to the MADS-box gene family. …”
Get full text

Transcriptomic analysis of Medicago truncatula calli with MtWOX9-1 overexpression by V. E. Tvorogova, E. Y. Krasnoperova, A. A. Kudriashov, K. A. Kuznetsova, E. A. Potsenkovskaya, Y. A. Fedorova, L. A. Lutova

“…Enriched GO pathways included  several groups  related to histone  methyltransferase activity as well as DNA methylation and chromatin binding,  suggesting major epigenetic changes that  occur in call overexpressing MtWOX9-1. …”
Get full text

ρ-Coumaric acid-zinc oxide nanoparticles improve post-thaw quality of goat spermatozoa and developmental competence of fertilized oocytes in vitro by Bahareh Akhtarshenas, Rasoul Kowsar, Mehdi Hajian, Nima Tanhaei Vash, Leila Soltani, Amir Hossein Mahdavi, Mohammad Hossein Nasr Esfahani

“…Moreover, using ρ-CA-ZnONPs significantly decreased lipid peroxidation and DNA damage compared with the other treatments. In conclusion, spermatozoa are cryotolerant, resistant to post-thaw conditions, and have fertilizing ability that can be increased by adding ρ-CA-ZnONPs as an antioxidant to goat semen extenders.…”
Get full text