IL-6–174 G/C transversion might decrease male infertility risk: A case-control study by Tayyebeh Zamani-Badi, Mohammad Karimian, Javad Amini Mahabadi, Atieh Rafatmanesh, Hossein Nikzad

“…Cases were categorized into 3 subgroups based on World Health Organization criteria: (i) nonobstructive azoospermia (n = 42), (ii) oligozoospermia (n = 61), and (iii) asthenozoospermia (n = 60). After DNA extraction, genotypes of the samples at the -174 G/C (rs1800795) locus were determined using the polymerase chain reaction-restriction fragment length polymorphism method. …”
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Inhibition of RAD51 by siRNA and Resveratrol Sensitizes Cancer Stem Cells Derived from HeLa Cell Cultures to Apoptosis by Graciela Ruíz, Heriberto A. Valencia-González, Ismael León-Galicia, Enrique García-Villa, Alejandro García-Carrancá, Patricio Gariglio

“…We hypothesized CSC repair more efficiently DNA breaks and that RAD51 plays an important role in this mechanism. …”
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Linfadenopatías generalizadas como manifestación inicial de lupus eritematoso sistémico: a propósito de un caso clínico by María Raad Sarabia, Gabriel Beltran Hoyos, Kewin Hernandez Pino, Jorge Acosta Buelva, Katty Chamorro Acevedo, Jorge Rico Fontalvo, Lourdes Carolina Vázquez Jiménez

“…La investigación de laboratorio finalmente reveló leucopenia, proteinuria significativa, ANA y anti-dsDNA positivos e hipocomplementemia, lo que confirma el diagnóstico de enfermedad autoinmune tipo LES. …”
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The complete mitochondrial genomes of five critical phytopathogenic Bipolaris species: features, evolution, and phylogeny by Xinzheng Song, Yuehua Geng, Chao Xu, Jiaxin Li, Yashuang Guo, Yan Shi, Qingzhou Ma, Qiang Li, Meng Zhang

“…The five mitogenomes were all circular DNA molecules, with lengths ranging from 106,403 bp to 135,790 bp. …”
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Characterizations of cytokines and viral genomes in serum of patients with Dabie bandavirus infection by Zefeng Dong, Man Yuan, Yueping Xing, Hongkai Zhang, Qiang Shen

“…The genomes of Dabie bandavirus were amplified using a designated primer pool. The DNA sequencing libraries were prepared using a ligation method. …”
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The Adducts Lipid Peroxidation Products with 2′-DeoxyNucleosides: A Theoretical Approach of Ionisation Potential by Boleslaw T. Karwowski

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Profil Ekspresi MikroRNA Kanker Payudara di Purwokerto by Ari Dwi Nurasih, Indah Sulistiyawati, Muhammad Falah

“…Metode: Analisis profil ekspresi mikroRNA pada kanker payudara dilakukan dengan isolasi plasma dari pasien kanker payudara dan individu sehat, sintesis cDNA, dan kuantifikasi mikroRNA dengan quantitative real time PCR (qPCR). …”
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Protein C deficiency with recurrent systemic thrombosis associated with compound heterozygous PROC missense variants by Mikio Shiba, Shuichiro Higo, Yu Morishita, Yasuhiro Ichibori, Yoshihiro Kin, Yasushi Sakata, Yoshiharu Higuchi

“…PCR amplification of genomic DNA spanning these exons followed by Sanger sequencing analysis revealed that the c.76G>A and the synonymous c.423G>T variants were in the same allele, whereas the c.1000G>A variant was on the opposite allele, indicating compound heterozygosity. …”
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Genetic Relationship Analysis of Genus Nomorhamphus from Lindu Lake, Central Sulawesi and Adaptive Responses to Exposure Different Light Wavelengths by Riva Hafidah, Dinar Tri Soelistyowati, Agus Oman Sudrajat, Alimuddin

“…Caudal fin of six fish from the three inlet rivers of Lake Lindu were preserved in 95% ethanol for DNA extraction purposes. This research used a completely randomized design with three treatment, namely rearing under white (400 nm), green (525 nm), and red (625 nm) light for 60 days. …”
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Intra-tumoral sphingobacterium multivorum promotes triple-negative breast cancer progression by suppressing tumor immunosurveillance by Zhikai Mai, Liwu Fu, Jiyan Su, Kenneth K.W. To, Chuansheng Yang, Chenglai Xia

“…However, the impact of specific intratumor-resident bacteria on tumor progression and their underlying mechanisms remain elusive. Methods 16S rDNA gene sequencing was used to analyze the cancerous and paracancerous tissues from breast cancer patients. …”
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PCR 和分离培养检测细胞培养支原体污染的比较 by 赖小敏　方国源　李彩霞　王传恩

“…理论上推测这对引物可扩增所有支原体 16S rDNA。但对大肠杆菌、变形杆菌及无支原体污染的组织培养 细胞等未见有扩增带出现。在对33份细胞培养标本的检测中, 除PCR 和分离培养同时检出 2份阳性外,前方法还另检出阳性 3 份。…”
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Nucleos(t)ide Analog Treatment Discontinuation in Chronic Hepatitis B Virus Infection: A Systematic Literature Review by Robert Gish, Kosh Agarwal, Anadi Mahajan, Supriya Desai, Saifuddin Kharawala, Rob Elston, Joyeta Das, Stuart Kendrick, Vera Gielen

“…Significant predictors with adequate evidence of clinical relapse included older age, male gender, and higher hepatitis B surface antigen (HBsAg) and hepatitis B virus DNA at baseline and end of treatment. HBsAg loss was reported in 25 studies, with overall median HBsAg loss rates ranging from 2% at 24 weeks (5 studies) to 11% at 192 weeks (2 studies) post NA cessation. …”
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Comparison of different developmental stages of jujube (Ziziphus jujuba) fruit and identification of Hub genes by Li Yalan, Ren Tian, Qu Siyu, Hao Qing, Fan Dingyu, Alimire Alimu

“…The number of DEGs in the four group comparisons WM vs FE, ER vs WM, FR vs ER and FR vs FE groups were 1576, 8561, 1073 and 7884 DEGs, respectively, and mainly involved in biological processes such as stimulation, defence, immunity, ADP binding, DNA-binding transcription factor activity, secondary active transmembrane transporter activity, etc. …”
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Phylogeny-Directed Search for Murine Leukemia Virus-Like Retroviruses in Vertebrate Genomes and in Patients Suffering from Myalgic Encephalomyelitis/Chronic Fatigue Syndrome and Pr... by Jonas Blomberg, Ali Sheikholvaezin, Amal Elfaitouri, Fredrik Blomberg, Anna Sjösten, Johan Mattson Ulfstedt, Rüdiger Pipkorn, Clas Källander, Christina Öhrmalm, Göran Sperber

“…One, MERV G3, contained MLVs and XMRV/HMRV. Its presence in mouse DNA, and the abundance of xenotropic MLVs in biologicals, is a source of false positivity. …”
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Plasmodium falciparum Merozoite Surface Proteins Polymorphisms and Treatment Outcomes among Patients with Uncomplicated Malaria in Mwanza, Tanzania by Karol J. Marwa, Eric Lyimo, Eveline T. Konje, Anthony Kapesa, Erasmus Kamugisha, Göte Swedberg

“…The influence of MOI on peripheral parasite clearance and adequate clinical and parasitological response (ACPR) was also assessed. Methods. Parasite DNA was extracted from dried blood spots according to the manufacture’s protocol. …”
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CONTEXTUAL LEARNING IN THE MODERN PRESCHOOL CURRICULUM by Danica Veselinov, Ljiljana Kelemen Milojević, Jasmina Mirković

“…Each kindergarten has its own identity, its DNA, just as children, preschool teachers and other participants in the educational process have their own individual equations. …”
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Defective homologous recombination and genomic instability predict increased responsiveness to carbon ion radiotherapy in pancreatic cancer by Brock J. Sishc, Janapriya Saha, Elizabeth M. Alves, Lianghao Ding, Huiming Lu, Shih-Ya Wang, Katy L. Swancutt, James H. Nicholson, Angelica Facoetti, Arnold Pompos, Mario Ciocca, Todd A. Aguilera, Michael D. Story, Anthony J. Davis

“…Increased radiosensitivity was linked to impaired DNA double-strand break (DSB) repair, particularly in cell lines with deficiencies in the homologous recombination (HR) repair pathway and/or elevated genomic instability from replication stress. …”
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DArTseq-based silicoDArT and SNP markers reveal the genetic diversity and population structure of Kenyan cashew (Anacardium occidentale L.) landraces. by Dennis Wamalabe Mukhebi, Pauline Wambui Gachanja, Diana Jepkoech Karan, Brenda Muthoni Kamau, Pauline Wangeci King'ori, Bicko Steve Juma, Wilton Mwema Mbinda

“…Cashew leaf samples were collected in Kwale, Kilifi and Lamu counties along coastal Kenya followed by DNA extraction. The reduced libraries were sequenced using Hiseq 2500 Illumina sequencer, and the SNPs called using DarTsoft14. …”
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Identification of a new fish trypanosome from the large yellow croaker (Larimichthys crocea) and description of its impact on host pathology, blood biochemical parameters and immun... by Yang Xiaoao, Qi Pengzhi, Tao Zhen, Zhang Qingwei, Wang Yanjie, Zhu Denghui, Yan Xiaojun, Fu Peipei, Guo Baoying

“…The parasite had significant morphological differences from Trypanosoma epinepheli Su, Feng, Jiang, Guo, Liu & Xu, 2014 and Trypanosoma carassii (Mitrofanov) Doflein, 1901. The 18S rDNA sequences of the trypanosome from L. crocea had the highest homology (98.4%) with T. carassii. …”
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Differential responses of Bradyrhizobium sp. SUTN9-2 to plant extracts and implications for endophytic interactions within different host plants by Teerana Greetatorn, Pakpoom Boonchuen, Pongdet Piromyou, Pongpan Songwattana, Jenjira Wongdee, Kamonluck Teamtisong, Nantakorn Boonkerd, Shusei Sato, Neung Teaumroong, Panlada Tittabutr

“…Abstract Bradyrhizobium sp. strain SUTN9-2 demonstrates cell enlargement, increased DNA content, and efficient nitrogen fixation in response to rice (Oryza sativa) extract. …”
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